BuviA.00169.c

DnaB domain protein helicase, C-terminal domain protein

CENTER ID: BuviA.00169.c
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00169.c.B1.GE35342 Full length( BuviA.00169.c ) 1 491
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00169.c.B1.PS01595 Full length( BuviA.00169.c ) 1 491

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.446
RefSeq: YP_001110081.1
UniProt: A4JV90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLQSETNRRR NEAVERRRED AEVAAVEAEE CAVAALFGEH GAAAFDTLSL RLRAEDFFVP AYRLVFESFG EIVDRGAKAD QATLVTHLKS KESFSDRARE LVEQAIVVSY SIENIESYAD AIVEKARARA IHAELQLVQK EIPRVGGDVR SADVLQRIDA ISLKFADRQT DDTLLKSASD GLSAVVARWE KIESGEVLGI KTGIDDLDDR LGGLHGGQLI ILAGRPSMGK TAKALNIAAH ASILSVDELS LDELVMNPDP SPDLSPMGAI FSLEMGQVQL TMRILSNIAN VEYRRLVRGE FDTSDWTNLN GAFVRYGRSN IHISQESYLT PASLRAKLRM LIQRTKRKLR YVIVDYLQLM DADGPSAGAN RTNDIGAISR GLKKIATEFD VPVIALSQLN RELEKRADKR PVMSDLRESG AIEADADVIL FIYRDEVYNP DSPHKGMAEL IIGKGRDAGL GMVPAHCQLE YQRFSSMPKG AYSYESAYGG S
NT Sequence
ATGCTACAAT CCGAAACAAA TCGTCGCAGG AATGAGGCCG TGGAACGTAG AAGGGAAGAC GCTGAGGTCG CCGCCGTTGA GGCGGAGGAG TGCGCAGTCG CCGCATTGTT TGGAGAGCAC GGCGCGGCTG CGTTCGACAC GCTCTCGCTG CGCTTGCGAG CGGAGGATTT CTTCGTTCCC GCCTACCGAC TTGTCTTTGA GTCGTTCGGC GAGATCGTTG ACCGTGGGGC CAAGGCGGAT CAGGCGACGC TTGTCACTCA TTTGAAGTCG AAAGAGTCTT TCTCCGACCG GGCGCGCGAA CTGGTCGAGC AAGCCATCGT GGTGTCGTAC TCGATCGAAA ACATCGAGAG CTACGCGGAT GCGATTGTGG AGAAAGCGCG CGCCCGCGCG ATTCATGCCG AGCTGCAACT CGTCCAGAAG GAAATCCCAC GCGTCGGGGG AGACGTCCGG TCCGCCGATG TGCTGCAGCG GATCGATGCG ATCTCGCTGA AGTTTGCAGA CCGACAGACG GACGACACAC TGCTGAAGTC GGCCAGCGAT GGCCTGAGTG CGGTTGTCGC GCGGTGGGAA AAGATCGAGA GCGGCGAAGT ACTCGGGATC AAAACCGGAA TCGATGACCT CGACGATCGG CTTGGTGGGC TACACGGCGG ACAGCTGATC ATCTTGGCGG GGCGGCCCAG CATGGGCAAG ACCGCGAAAG CACTCAATAT TGCGGCTCAC GCCTCGATTC TCTCGGTCGA CGAGCTCTCG CTCGATGAGC TGGTGATGAA TCCGGACCCG TCGCCGGATC TGAGCCCGAT GGGCGCGATA TTTTCGTTGG AAATGGGGCA GGTCCAACTG ACTATGCGGA TCCTCTCCAA CATCGCTAAC GTTGAGTATC GTCGTCTGGT TCGTGGCGAG TTCGATACGA GCGATTGGAC CAATCTCAAC GGCGCATTCG TGCGCTACGG CCGCTCCAAC ATCCACATCA GTCAAGAGTC CTACCTTACG CCGGCGTCAC TTCGGGCAAA GCTCCGGATG CTGATCCAGC GAACGAAGCG AAAGCTCCGA TATGTCATCG TCGATTACCT GCAGCTGATG GATGCCGACG GACCCTCCGC CGGTGCAAAC CGAACGAACG ACATCGGTGC GATCTCGCGT GGGCTCAAGA AGATTGCAAC CGAATTCGAC GTTCCCGTTA TCGCGCTGTC GCAGCTAAAT CGCGAGCTCG AGAAGCGAGC TGACAAGAGA CCCGTGATGT CGGACCTGCG CGAGTCCGGC GCCATTGAGG CGGACGCTGA CGTGATCCTG TTTATCTACC GCGACGAAGT TTACAACCCC GATTCACCGC ATAAGGGAAT GGCCGAACTC ATTATCGGCA AGGGGCGTGA CGCGGGACTC GGGATGGTAC CGGCACACTG TCAACTCGAA TACCAACGTT TCTCGTCGAT GCCGAAAGGC GCTTACTCGT ACGAATCCGC ATACGGAGGA TCT
Details for BuviA.00169.c.B1.GE35342
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 51
Validated AA Sequence
MGKTAKALNI AAHASILSVD ELSLDELVMN PDPSPDLSPM GAIFSLEMGQ VQLTMRILSN IANVEYRRLV RGEFDTSDWT NLNGAFVRYG RSNIHISQES YLTPASLRAK LRMLIQRTKR KLRYVIVDYL QLMDADGPSA GANRTNDIGA ISRGLKKIAT EFDVPVIALS QLNRELEKRA DKRPVMSDLR ESGAIEADAD VILFIYRDEV YNPDSPHKGM AELIIGKGRD AGLGMVPAHC QLEYQRFSSM PKGAY
Validated NT Sequence
gagtaagcgc ctttcggcat cgacgagaaa cgttggtatt cgagttgaca gtgtgccggt accatcccga gtcccgcgtc acgccccttg ccgataatga gttcggccat tcccttatgc ggtgaatcgg ggttgtaaac ttcgtcgcgg tagataaaca ggatcacgtc agcgtccgcc tcaatggcgc cggactcgcg caggtccgac atcacgggtc tcttgtcagc tcgcttctcg agctcgcgat ttagctgcga cagcgcgata acgggaacgt cgaattcggt tgcaatcttc ttgagcccac gcgagatcgc accgatgtcg ttcgttcggt ttgcaccggc ggagggtccg tcggcatcca tcagctgcag gtaatcgacg atgacatatc ggagctttcg cttcgttcgc tggatcagca tccggagctt tgcccgaagt gacgccggcg taaggtagga ctcttgactg atgtggatgt tggagcggcc gtagcgcacg aatgcgccgt tgagattggt ccaatcgctc gtatcgaact cgccacgaac cagacgacga tactcaacgt tagcgatgtt ggagaggatc cgcatagtca gttggacctg ccccatttcc aacgaaaata tcgcgcccat cgggctcaga tccggcgacg ggtccggatt catcaccagc tcatcgagcg agagctcgtc gaccgagaga atcgaggcgt gagccgcaat attgagtgct ttcgcggtct tgcccatgct gggccgcccc gccaagatga tcagctgtcc gccgtgtagc ccaccaagcc gatcgtcgag gtcatcgatt ccggttttga tcccgagtac ttcgccgctc tcgatctttt cccaccgcgc gacaaccgca ctcaggccat cgctggccga cttcagcagt gtgtcgtccg tctgtcggtc tgcaaacttc agcgagatcg catcgatccg ctgcagcaca tcggcggacc ggacgtctcc cccgacgcgt gggatttcct tctggacgag ttgcagctcg gcatgaatcg cgcgggcgcg cgctttctcc acaatcgcat ccgcgtagct ctcgatgttt tcgatcgagt acgacaccac gatggcttgc tcgaccagtt cgcgcgcccg gtcggagaaa gactctttcg acttcaaatg agtgacaagc gtcgcctgat ccgccttggc cccacggtca acgatctcgc cgaacgactc aaagacaagt cggtaggcgg gaacgaagaa atcctccgct cgcaagcgca gcgagagcgt gtcgaacgca gccgcgccgt gctctccaaa caatgcggcg actgcgcact cctccgcctc aacggcggcg acctcagcgt cttcccttct acgttccacg gcctcattcc tgcgacgatt tgtttcggat tg
Expected Protein Sequence
MAHHHHHHML QSETNRRRNE AVERRREDAE VAAVEAEECA VAALFGEHGA AAFDTLSLRL RAEDFFVPAY RLVFESFGEI VDRGAKADQA TLVTHLKSKE SFSDRARELV EQAIVVSYSI ENIESYADAI VEKARARAIH AELQLVQKEI PRVGGDVRSA DVLQRIDAIS LKFADRQTDD TLLKSASDGL SAVVARWEKI ESGEVLGIKT GIDDLDDRLG GLHGGQLIIL AGRPSMGKTA KALNIAAHAS ILSVDELSLD ELVMNPDPSP DLSPMGAIFS LEMGQVQLTM RILSNIANVE YRRLVRGEFD TSDWTNLNGA FVRYGRSNIH ISQESYLTPA SLRAKLRMLI QRTKRKLRYV IVDYLQLMDA DGPSAGANRT NDIGAISRGL KKIATEFDVP VIALSQLNRE LEKRADKRPV MSDLRESGAI EADADVILFI YRDEVYNPDS PHKGMAELII GKGRDAGLGM VPAHCQLEYQ RFSSMPKGAY SYESAYGGS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctac aatccgaaac aaatcgtcgc aggaatgagg ccgtggaacg tagaagggaa gacgctgagg tcgccgccgt tgaggcggag gagtgcgcag tcgccgcatt gtttggagag cacggcgcgg ctgcgttcga cacgctctcg ctgcgcttgc gagcggagga tttcttcgtt cccgcctacc gacttgtctt tgagtcgttc ggcgagatcg ttgaccgtgg ggccaaggcg gatcaggcga cgcttgtcac tcatttgaag tcgaaagagt ctttctccga ccgggcgcgc gaactggtcg agcaagccat cgtggtgtcg tactcgatcg aaaacatcga gagctacgcg gatgcgattg tggagaaagc gcgcgcccgc gcgattcatg ccgagctgca actcgtccag aaggaaatcc cacgcgtcgg gggagacgtc cggtccgccg atgtgctgca gcggatcgat gcgatctcgc tgaagtttgc agaccgacag acggacgaca cactgctgaa gtcggccagc gatggcctga gtgcggttgt cgcgcggtgg gaaaagatcg agagcggcga agtactcggg atcaaaaccg gaatcgatga cctcgacgat cggcttggtg ggctacacgg cggacagctg atcatcttgg cggggcggcc cagcatgggc aagaccgcga aagcactcaa tattgcggct cacgcctcga ttctctcggt cgacgagctc tcgctcgatg agctggtgat gaatccggac ccgtcgccgg atctgagccc gatgggcgcg atattttcgt tggaaatggg gcaggtccaa ctgactatgc ggatcctctc caacatcgct aacgttgagt atcgtcgtct ggttcgtggc gagttcgata cgagcgattg gaccaatctc aacggcgcat tcgtgcgcta cggccgctcc aacatccaca tcagtcaaga gtcctacctt acgccggcgt cacttcgggc aaagctccgg atgctgatcc agcgaacgaa gcgaaagctc cgatatgtca tcgtcgatta cctgcagctg atggatgccg acggaccctc cgccggtgca aaccgaacga acgacatcgg tgcgatctcg cgtgggctca agaagattgc aaccgaattc gacgttcccg ttatcgcgct gtcgcagcta aatcgcgagc tcgagaagcg agctgacaag agacccgtga tgtcggacct gcgcgagtcc ggcgccattg aggcggacgc tgacgtgatc ctgtttatct accgcgacga agtttacaac cccgattcac cgcataaggg aatggccgaa ctcattatcg gcaaggggcg tgacgcggga ctcgggatgg taccggcaca ctgtcaactc gaataccaac gtttctcgtc gatgccgaaa ggcgcttact cgtacgaatc cgcatacgga ggatcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuviA.00169.c.B1.PS01595
PURIFICATION DATe: 8/15/2012
CONCENTRATION: 6.86mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MGKTAKALNI AAHASILSVD ELSLDELVMN PDPSPDLSPM GAIFSLEMGQ VQLTMRILSN IANVEYRRLV RGEFDTSDWT NLNGAFVRYG RSNIHISQES YLTPASLRAK LRMLIQRTKR KLRYVIVDYL QLMDADGPSA GANRTNDIGA ISRGLKKIAT EFDVPVIALS QLNRELEKRA DKRPVMSDLR ESGAIEADAD VILFIYRDEV YNPDSPHKGM AELIIGKGRD AGLGMVPAHC QLEYQRFSSM PKGAY
Validated NT Sequence
gagtaagcgc ctttcggcat cgacgagaaa cgttggtatt cgagttgaca gtgtgccggt accatcccga gtcccgcgtc acgccccttg ccgataatga gttcggccat tcccttatgc ggtgaatcgg ggttgtaaac ttcgtcgcgg tagataaaca ggatcacgtc agcgtccgcc tcaatggcgc cggactcgcg caggtccgac atcacgggtc tcttgtcagc tcgcttctcg agctcgcgat ttagctgcga cagcgcgata acgggaacgt cgaattcggt tgcaatcttc ttgagcccac gcgagatcgc accgatgtcg ttcgttcggt ttgcaccggc ggagggtccg tcggcatcca tcagctgcag gtaatcgacg atgacatatc ggagctttcg cttcgttcgc tggatcagca tccggagctt tgcccgaagt gacgccggcg taaggtagga ctcttgactg atgtggatgt tggagcggcc gtagcgcacg aatgcgccgt tgagattggt ccaatcgctc gtatcgaact cgccacgaac cagacgacga tactcaacgt tagcgatgtt ggagaggatc cgcatagtca gttggacctg ccccatttcc aacgaaaata tcgcgcccat cgggctcaga tccggcgacg ggtccggatt catcaccagc tcatcgagcg agagctcgtc gaccgagaga atcgaggcgt gagccgcaat attgagtgct ttcgcggtct tgcccatgct gggccgcccc gccaagatga tcagctgtcc gccgtgtagc ccaccaagcc gatcgtcgag gtcatcgatt ccggttttga tcccgagtac ttcgccgctc tcgatctttt cccaccgcgc gacaaccgca ctcaggccat cgctggccga cttcagcagt gtgtcgtccg tctgtcggtc tgcaaacttc agcgagatcg catcgatccg ctgcagcaca tcggcggacc ggacgtctcc cccgacgcgt gggatttcct tctggacgag ttgcagctcg gcatgaatcg cgcgggcgcg cgctttctcc acaatcgcat ccgcgtagct ctcgatgttt tcgatcgagt acgacaccac gatggcttgc tcgaccagtt cgcgcgcccg gtcggagaaa gactctttcg acttcaaatg agtgacaagc gtcgcctgat ccgccttggc cccacggtca acgatctcgc cgaacgactc aaagacaagt cggtaggcgg gaacgaagaa atcctccgct cgcaagcgca gcgagagcgt gtcgaacgca gccgcgccgt gctctccaaa caatgcggcg actgcgcact cctccgcctc aacggcggcg acctcagcgt cttcccttct acgttccacg gcctcattcc tgcgacgatt tgtttcggat tg
Expressed Protein Sequence
MAHHHHHHML QSETNRRRNE AVERRREDAE VAAVEAEECA VAALFGEHGA AAFDTLSLRL RAEDFFVPAY RLVFESFGEI VDRGAKADQA TLVTHLKSKE SFSDRARELV EQAIVVSYSI ENIESYADAI VEKARARAIH AELQLVQKEI PRVGGDVRSA DVLQRIDAIS LKFADRQTDD TLLKSASDGL SAVVARWEKI ESGEVLGIKT GIDDLDDRLG GLHGGQLIIL AGRPSMGKTA KALNIAAHAS ILSVDELSLD ELVMNPDPSP DLSPMGAIFS LEMGQVQLTM RILSNIANVE YRRLVRGEFD TSDWTNLNGA FVRYGRSNIH ISQESYLTPA SLRAKLRMLI QRTKRKLRYV IVDYLQLMDA DGPSAGANRT NDIGAISRGL KKIATEFDVP VIALSQLNRE LEKRADKRPV MSDLRESGAI EADADVILFI YRDEVYNPDS PHKGMAELII GKGRDAGLGM VPAHCQLEYQ RFSSMPKGAY SYESAYGGS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTAC AATCCGAAAC AAATCGTCGC AGGAATGAGG CCGTGGAACG TAGAAGGGAA GACGCTGAGG TCGCCGCCGT TGAGGCGGAG GAGTGCGCAG TCGCCGCATT GTTTGGAGAG CACGGCGCGG CTGCGTTCGA CACGCTCTCG CTGCGCTTGC GAGCGGAGGA TTTCTTCGTT CCCGCCTACC GACTTGTCTT TGAGTCGTTC GGCGAGATCG TTGACCGTGG GGCCAAGGCG GATCAGGCGA CGCTTGTCAC TCATTTGAAG TCGAAAGAGT CTTTCTCCGA CCGGGCGCGC GAACTGGTCG AGCAAGCCAT CGTGGTGTCG TACTCGATCG AAAACATCGA GAGCTACGCG GATGCGATTG TGGAGAAAGC GCGCGCCCGC GCGATTCATG CCGAGCTGCA ACTCGTCCAG AAGGAAATCC CACGCGTCGG GGGAGACGTC CGGTCCGCCG ATGTGCTGCA GCGGATCGAT GCGATCTCGC TGAAGTTTGC AGACCGACAG ACGGACGACA CACTGCTGAA GTCGGCCAGC GATGGCCTGA GTGCGGTTGT CGCGCGGTGG GAAAAGATCG AGAGCGGCGA AGTACTCGGG ATCAAAACCG GAATCGATGA CCTCGACGAT CGGCTTGGTG GGCTACACGG CGGACAGCTG ATCATCTTGG CGGGGCGGCC CAGCATGGGC AAGACCGCGA AAGCACTCAA TATTGCGGCT CACGCCTCGA TTCTCTCGGT CGACGAGCTC TCGCTCGATG AGCTGGTGAT GAATCCGGAC CCGTCGCCGG ATCTGAGCCC GATGGGCGCG ATATTTTCGT TGGAAATGGG GCAGGTCCAA CTGACTATGC GGATCCTCTC CAACATCGCT AACGTTGAGT ATCGTCGTCT GGTTCGTGGC GAGTTCGATA CGAGCGATTG GACCAATCTC AACGGCGCAT TCGTGCGCTA CGGCCGCTCC AACATCCACA TCAGTCAAGA GTCCTACCTT ACGCCGGCGT CACTTCGGGC AAAGCTCCGG ATGCTGATCC AGCGAACGAA GCGAAAGCTC CGATATGTCA TCGTCGATTA CCTGCAGCTG ATGGATGCCG ACGGACCCTC CGCCGGTGCA AACCGAACGA ACGACATCGG TGCGATCTCG CGTGGGCTCA AGAAGATTGC AACCGAATTC GACGTTCCCG TTATCGCGCT GTCGCAGCTA AATCGCGAGC TCGAGAAGCG AGCTGACAAG AGACCCGTGA TGTCGGACCT GCGCGAGTCC GGCGCCATTG AGGCGGACGC TGACGTGATC CTGTTTATCT ACCGCGACGA AGTTTACAAC CCCGATTCAC CGCATAAGGG AATGGCCGAA CTCATTATCG GCAAGGGGCG TGACGCGGGA CTCGGGATGG TACCGGCACA CTGTCAACTC GAATACCAAC GTTTCTCGTC GATGCCGAAA GGCGCTTACT CGTACGAATC CGCATACGGA GGATCTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T