BuviA.00169.a

DnaB domain protein helicase, C-terminal domain protein

CENTER ID: BuviA.00169.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00169.a.B1.GE35344 Full length( BuviA.00169.a ) 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.5399
RefSeq: YP_001119009.1
UniProt: A4JD21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSANDLAKAV PHSVEAEQAV IGILLNDNDA IDRIGDLRTE HFFRGDHRAL FAEIVGLIAN GVGVDIVSLF DRLRSKGKAD DVGGLSYLNA LAQNAPSAVS IARNAAIVRD RAQKRGLLAV ASEMQESVAG TADDAGTLID RAAAKLEELG EATVKREPKL LAQALTDHIN LLERRSTGGE RVISTGYDDL DRALNGGLRP GWSLILAARP GMGKTSLALN IATHAAADHG VLFLSMEMPE SELIDRNIAS LGRVPLDRVM NAPDDNEFWD RVTAATLKMR DMNLHIDDQP ALRLLDVRTK ARMVKRKSGL DVLIVDYLQL MQGEGANRNA EIEGISRGLK ALAKELNIAV IALAQLNRQV EQRANRTPML SDLRDSGSIE QDADAVLFIH REEVANPDAG EQWRGFAQIR VAKFRHGRTG DVPMTYSGEF VRFENHSGAW PTHTVQKSTR SRGFE
NT Sequence
ATGAGCGCAA ACGACCTTGC AAAGGCCGTG CCGCATAGCG TCGAGGCCGA GCAGGCAGTG ATCGGCATTC TGCTCAACGA CAACGACGCG ATCGACCGGA TTGGCGATCT GCGCACGGAG CATTTTTTTC GCGGCGACCA CCGCGCACTG TTCGCCGAGA TCGTCGGGCT GATCGCTAAT GGCGTCGGCG TCGACATCGT GAGCCTGTTC GACAGGTTGC GCTCGAAGGG CAAGGCCGAT GATGTCGGCG GCCTTTCATA CCTGAACGCG CTGGCGCAGA ACGCACCAAG CGCGGTGTCG ATCGCGCGAA ACGCAGCGAT TGTCCGCGAC CGGGCACAGA AGCGGGGACT GCTGGCCGTC GCATCGGAGA TGCAGGAATC GGTCGCTGGT ACCGCTGACG ACGCCGGTAC GCTCATCGAT CGAGCCGCGG CAAAGTTGGA GGAGCTCGGC GAAGCGACGG TGAAGCGTGA GCCGAAGCTG TTGGCCCAGG CACTGACCGA CCACATCAAC CTGCTGGAAC GCCGATCGAC AGGAGGTGAG CGCGTTATCT CAACGGGCTA CGACGATCTT GACCGCGCGC TAAACGGTGG ATTGCGTCCG GGCTGGTCGC TCATCTTGGC AGCTCGTCCT GGCATGGGGA AAACGAGTCT CGCGCTTAAC ATCGCGACCC ACGCGGCTGC CGACCACGGT GTCCTGTTTC TATCGATGGA GATGCCGGAG AGCGAGCTGA TCGACCGAAA CATCGCGTCG CTCGGCCGCG TTCCGCTCGA TCGGGTGATG AACGCGCCGG ACGACAACGA GTTCTGGGAT CGCGTGACGG CCGCAACGCT GAAGATGCGC GATATGAACC TGCACATCGA CGACCAACCG GCGCTGCGTC TGCTCGACGT TCGGACCAAG GCTCGGATGG TCAAGCGCAA GTCGGGTCTC GACGTGCTGA TCGTCGACTA CCTGCAGCTC ATGCAGGGCG AAGGCGCGAA CCGCAACGCG GAAATCGAGG GAATCTCGCG TGGCTTGAAG GCACTCGCGA AGGAATTGAA CATTGCCGTG ATCGCCCTGG CTCAGCTGAA CCGGCAGGTC GAGCAACGGG CGAACCGCAC GCCGATGCTG TCTGACCTGC GCGACTCCGG ATCGATTGAG CAGGATGCAG ATGCCGTGCT GTTCATCCAC CGGGAAGAAG TAGCCAACCC GGACGCCGGC GAGCAGTGGC GCGGCTTTGC TCAGATTCGC GTCGCCAAGT TCCGGCATGG ACGCACGGGA GACGTTCCGA TGACATACAG CGGTGAGTTT GTGAGGTTTG AGAACCATTC CGGCGCATGG CCGACACATA CCGTTCAAAA ATCCACGCGC TCGAGGGGGT TCGAA
Details for BuviA.00169.a.B1.GE35344
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 69
Validated AA Sequence
MQESVAGTAD DAGTLIDRAA AKLEELGEAT VKREPKLLAQ ALTDHINLLE RRSTGGERVI STGYDDLDRA LNGGLRPGWS LILAARPGMG KTSLALNIAT HAAADHGVLF LSMEMPESEL IDRNIASLGR VPLDRVMNAP DDNEFWDRVT AATLKMRDMN LHIDDQPALR LLDVRTKARM VKRKSGLDVL IVDYLQLMQG EGANRNAEIE GISRGLKALA KELNIAVIAL AQLNRQVEQR ANRTPMLSDL RDSGSIEQDA DAVLFIHREE VANPDAGEQW RGFAQIRVAK FRHGRTGDVP MTYSGEFVRF ENHSGAWPTH TVQ
Validated NT Sequence
ttttgaacgg tatgtgtcgg ccatgcgccg gaatggttct caaacctcac aaactcaccg ctgtatgtca tcggaacgtc tcccgtgcgt ccatgccgga acttggcgac gcgaatctga gcaaagccgc gccactgctc gccggcgtcc gggttggcta cttcttcccg gtggatgaac agcacggcat ctgcatcctg ctcaatcgat ccggagtcgc gcaggtcaga cagcatcggc gtgcggttcg cccgttgctc gacctgccgg ttcagctgag ccagggcgat cacggcaatg ttcaattcct tcgcgagtgc cttcaagcca cgcgagattc cctcgatttc cgcgttgcgg ttcgcgcctt cgccctgcat gagctgcagg tagtcgacga tcagcacgtc gagacccgac ttgcgcttga ccatccgagc cttggtccga acgtcgagca gacgcagcgc cggttggtcg tcgatgtgca ggttcatatc gcgcatcttc agcgttgcgg ccgtcacgcg atcccagaac tcgttgtcgt ccggcgcgtt catcacccga tcgagcggaa cgcggccgag cgacgcgatg tttcggtcga tcagctcgct ctccggcatc tccatcgata gaaacaggac accgtggtcg gcagccgcgt gggtcgcgat gttaagcgcg agactcgttt tccccatgcc aggacgagct gccaagatga gcgaccagcc cggacgcaat ccaccgttta gcgcgcggtc aagatcgtcg tagcccgttg agataacgcg ctcacctcct gtcgatcggc gttccagcag gttgatgtgg tcggtcagtg cctgggccaa cagcttcggc tcacgcttca ccgtcgcttc gccgagctcc tccaactttg ccgcggctcg atcgatgagc gtaccggcgt cgtcagcggt accagcgacc gattcctgca tctccgatgc gacggccagc agtccccgct tctgtgcccg gtcgcggaca atcgctgcgt ttcgcgcgat cgacaccgcg cttggtgcgt tctgcgccag cgcgttcagg tatgaaaggc cgccgacatc atcggccttg cccttcgagc gcaacctgtc gaacaggctc acgatgtcga cgccgacgcc attagcgatc agcccgacga tctcggcgaa cagtgcgcgg tggtcgccgc gaaaaaaatg ctccgtgcgc agatcgccaa tccggtcgat cgcgtcgttg tcgttgagca gaatgccgat cactgcctgc tcggcctcga cgctatgcgg cacggccttt gcaaggtcgt tt
Expected Protein Sequence
MAHHHHHHMS ANDLAKAVPH SVEAEQAVIG ILLNDNDAID RIGDLRTEHF FRGDHRALFA EIVGLIANGV GVDIVSLFDR LRSKGKADDV GGLSYLNALA QNAPSAVSIA RNAAIVRDRA QKRGLLAVAS EMQESVAGTA DDAGTLIDRA AAKLEELGEA TVKREPKLLA QALTDHINLL ERRSTGGERV ISTGYDDLDR ALNGGLRPGW SLILAARPGM GKTSLALNIA THAAADHGVL FLSMEMPESE LIDRNIASLG RVPLDRVMNA PDDNEFWDRV TAATLKMRDM NLHIDDQPAL RLLDVRTKAR MVKRKSGLDV LIVDYLQLMQ GEGANRNAEI EGISRGLKAL AKELNIAVIA LAQLNRQVEQ RANRTPMLSD LRDSGSIEQD ADAVLFIHRE EVANPDAGEQ WRGFAQIRVA KFRHGRTGDV PMTYSGEFVR FENHSGAWPT HTVQKSTRSR GFE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg caaacgacct tgcaaaggcc gtgccgcata gcgtcgaggc cgagcaggca gtgatcggca ttctgctcaa cgacaacgac gcgatcgacc ggattggcga tctgcgcacg gagcattttt ttcgcggcga ccaccgcgca ctgttcgccg agatcgtcgg gctgatcgct aatggcgtcg gcgtcgacat cgtgagcctg ttcgacaggt tgcgctcgaa gggcaaggcc gatgatgtcg gcggcctttc atacctgaac gcgctggcgc agaacgcacc aagcgcggtg tcgatcgcgc gaaacgcagc gattgtccgc gaccgggcac agaagcgggg actgctggcc gtcgcatcgg agatgcagga atcggtcgct ggtaccgctg acgacgccgg tacgctcatc gatcgagccg cggcaaagtt ggaggagctc ggcgaagcga cggtgaagcg tgagccgaag ctgttggccc aggcactgac cgaccacatc aacctgctgg aacgccgatc gacaggaggt gagcgcgtta tctcaacggg ctacgacgat cttgaccgcg cgctaaacgg tggattgcgt ccgggctggt cgctcatctt ggcagctcgt cctggcatgg ggaaaacgag tctcgcgctt aacatcgcga cccacgcggc tgccgaccac ggtgtcctgt ttctatcgat ggagatgccg gagagcgagc tgatcgaccg aaacatcgcg tcgctcggcc gcgttccgct cgatcgggtg atgaacgcgc cggacgacaa cgagttctgg gatcgcgtga cggccgcaac gctgaagatg cgcgatatga acctgcacat cgacgaccaa ccggcgctgc gtctgctcga cgttcggacc aaggctcgga tggtcaagcg caagtcgggt ctcgacgtgc tgatcgtcga ctacctgcag ctcatgcagg gcgaaggcgc gaaccgcaac gcggaaatcg agggaatctc gcgtggcttg aaggcactcg cgaaggaatt gaacattgcc gtgatcgccc tggctcagct gaaccggcag gtcgagcaac gggcgaaccg cacgccgatg ctgtctgacc tgcgcgactc cggatcgatt gagcaggatg cagatgccgt gctgttcatc caccgggaag aagtagccaa cccggacgcc ggcgagcagt ggcgcggctt tgctcagatt cgcgtcgcca agttccggca tggacgcacg ggagacgttc cgatgacata cagcggtgag tttgtgaggt ttgagaacca ttccggcgca tggccgacac ataccgttca aaaatccacg cgctcgaggg ggttcgaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat