BuviA.00166.a

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase

CENTER ID: BuviA.00166.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00166.a.B1.GE35349 Full length( BuviA.00166.a ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4738
RefSeq: YP_001118389.1
UniProt: A4JBA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKQRTIKSI VKTVGIGLHS GRKVELTLRP AAPGTGIVFS RVDLPTPVDI PASAMSIGDT RLASVLQKDG ARVSTVEHLM SACAGLGIDN LYVDVTAEEI PIMDGSAASF VFLIQSAGIE EQNAPKRFIK VTKPVEIRDG DKFARLDPFF GFKLKFTIDF RHPAVDKTGQ ELEVDFANTS YVREIARART FGFAHEVEMM RELGLARGGS MDNAIVLDEY RILNNDGLRY DDEFVKHKML DAIGDLYVVG HPLLASYTAY KSGHGLNNAL LRELLAHEDA YEIVTFDDPQ SAPKGFAFDT QTAFA
NT Sequence
ATGCTGAAGC AGCGCACCAT CAAATCGATC GTGAAGACCG TGGGTATCGG TCTGCACTCG GGCCGCAAGG TCGAACTGAC GCTCCGCCCG GCCGCGCCGG GCACGGGGAT CGTGTTCTCG CGCGTCGATT TGCCGACGCC CGTCGACATC CCGGCCTCCG CGATGTCGAT CGGCGATACG CGGCTCGCGT CTGTGCTGCA GAAGGACGGT GCGCGCGTGT CGACCGTCGA GCACCTGATG TCCGCGTGTG CGGGCCTGGG CATCGACAAT CTCTATGTCG ACGTGACGGC CGAGGAAATT CCGATCATGG ACGGCAGCGC CGCGTCCTTC GTGTTCCTGA TCCAGTCGGC GGGCATCGAG GAGCAGAACG CGCCGAAGCG CTTCATCAAG GTGACGAAGC CGGTCGAAAT CCGCGATGGT GACAAGTTCG CGCGCCTCGA CCCGTTTTTC GGCTTCAAGC TGAAGTTCAC GATCGACTTC CGTCACCCGG CCGTGGACAA GACCGGCCAG GAACTCGAGG TCGATTTCGC GAATACGTCG TACGTGCGCG AGATCGCGCG TGCGCGCACG TTCGGTTTCG CGCACGAGGT CGAGATGATG CGCGAGCTGG GCCTCGCACG CGGCGGCAGC ATGGACAACG CGATCGTGCT CGACGAATAC CGGATCCTCA ACAACGACGG CTTGCGCTAC GACGACGAGT TCGTGAAGCA CAAGATGCTC GACGCGATCG GCGACCTGTA CGTGGTCGGC CATCCGCTGC TGGCATCGTA CACGGCTTAC AAGTCGGGCC ACGGGCTGAA CAACGCGCTG CTGCGCGAAC TGCTCGCGCA CGAAGACGCC TACGAGATCG TCACGTTCGA CGATCCGCAA TCCGCACCGA AGGGGTTCGC GTTCGATACG CAGACGGCCT TCGCA
Details for BuviA.00166.a.B1.GE35349
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 34kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KQRTIKSIVK TVGIGLHSGR KVELTLRPAA PGTGIVFSRV DLPTPVDIPA SAMSIGDTRL ASVLQKDGAR VSTVEHLMSA CAGLGIDNLY VDVTAEEIPI MDGSAASFVF LIQSAGIEEQ NAPKRFIKVT KPVEIRDGDK FARLDPFFGF KLKFTIDFRH PAVDKTGQEL EVDFANTSYV REIARARTFG FAHEVEMMRE LGLARGGSMD NAIVLDEYRI LNNDGLRYDD EFVKHKMLDA IGDLYVVGHP LLASYTAYKS GHGLNNALLR ELLAHEDAYE IVTFDDPQSA PXGVRVRYAX GLRXSE
Validated NT Sequence
ttgtnnnnnt taagaaggag atataccatg gctcaccacc accaccacca tatgctgaag cagcgcacca tcaaatcgat cgtgaagacc gtgggtatcg gtctgcactc gggccgcaag gtcgaactga cgctccgccc ggccgcgccg ggcacgggga tcgtgttctc gcgcgtcgat ttgccgacgc ccgtcgacat cccggcctcc gcgatgtcga tcggcgatac gcggctcgcg tctgtgctgc agaaggacgg tgcgcgcgtg tcgaccgtcg agcacctgat gtccgcgtgt gcgggcctgg gcatcgacaa tctctatgtc gacgtgacgg ccgaggaaat tccgatcatg gacggcagcg ccgcgtcctt cgtgttcctg atccagtcgg cgggcatcga ggagcagaac gcgccgaagc gcttcatcaa ggtgacgaag ccggtcgaaa tccgcgatgg tgacaagttc gcgcgcctcg acccgttttt cggcttcaag ctgaagttca cgatcgactt ccgtcacccg gccgtggaca agaccggcca ggaactcgag gtcgatttcg cgaatacgtc gtacgtgcgc gagatcgcgc gtgcgcgcac gttcggtttc gcgcacgagg tcgagatgat gcgcgagctg ggcctcgcac gcggcggcag catggacaac gcgatcgtgc tcgacgaata ccggatcctc aacaacgacg gcttgcgcta cgacgacgag ttcgtgaagc acaagatgct cgacgcgatc ggcgacctgt acgtggtcgg ccatccgctg ctggcatcgt acacggctta caagtcgggc cacgggctga acaacgcgct gctgcgcgaa ctgctcgcgc acgaagacgc ctacgagatc gtcacgttcg acgatccgca atccgcaccg aanggggttc gcgttcgata cgcaganggc cttcgcatna gtgagtaaga tagnatnngn ntnnnnnnnn nc
Expected Protein Sequence
MAHHHHHHML KQRTIKSIVK TVGIGLHSGR KVELTLRPAA PGTGIVFSRV DLPTPVDIPA SAMSIGDTRL ASVLQKDGAR VSTVEHLMSA CAGLGIDNLY VDVTAEEIPI MDGSAASFVF LIQSAGIEEQ NAPKRFIKVT KPVEIRDGDK FARLDPFFGF KLKFTIDFRH PAVDKTGQEL EVDFANTSYV REIARARTFG FAHEVEMMRE LGLARGGSMD NAIVLDEYRI LNNDGLRYDD EFVKHKMLDA IGDLYVVGHP LLASYTAYKS GHGLNNALLR ELLAHEDAYE IVTFDDPQSA PKGFAFDTQT AFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctga agcagcgcac catcaaatcg atcgtgaaga ccgtgggtat cggtctgcac tcgggccgca aggtcgaact gacgctccgc ccggccgcgc cgggcacggg gatcgtgttc tcgcgcgtcg atttgccgac gcccgtcgac atcccggcct ccgcgatgtc gatcggcgat acgcggctcg cgtctgtgct gcagaaggac ggtgcgcgcg tgtcgaccgt cgagcacctg atgtccgcgt gtgcgggcct gggcatcgac aatctctatg tcgacgtgac ggccgaggaa attccgatca tggacggcag cgccgcgtcc ttcgtgttcc tgatccagtc ggcgggcatc gaggagcaga acgcgccgaa gcgcttcatc aaggtgacga agccggtcga aatccgcgat ggtgacaagt tcgcgcgcct cgacccgttt ttcggcttca agctgaagtt cacgatcgac ttccgtcacc cggccgtgga caagaccggc caggaactcg aggtcgattt cgcgaatacg tcgtacgtgc gcgagatcgc gcgtgcgcgc acgttcggtt tcgcgcacga ggtcgagatg atgcgcgagc tgggcctcgc acgcggcggc agcatggaca acgcgatcgt gctcgacgaa taccggatcc tcaacaacga cggcttgcgc tacgacgacg agttcgtgaa gcacaagatg ctcgacgcga tcggcgacct gtacgtggtc ggccatccgc tgctggcatc gtacacggct tacaagtcgg gccacgggct gaacaacgcg ctgctgcgcg aactgctcgc gcacgaagac gcctacgaga tcgtcacgtt cgacgatccg caatccgcac cgaaggggtt cgcgttcgat acgcagacgg ccttcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat