SSGCID: Target Page

BuviA.00158.a

Glutamate racemase

CENTER ID:	BuviA.00158.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00158.a.B1.GE39172	Full length( BuviA.00158.a )		1	286

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00158.a.B1.PW37799	Full length( BuviA.00158.a )		1	286

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6522
RefSeq:	YP_001120116.1
UniProt:	A4JG78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTNRSTASA ARPTAPIGIF DSGLGGLSVL RAVRAQLPGE SFVYVADSRH APYGPRDEAF ITERTLAICE WLARDGAKAL VVACNTATAR AIAAVRERLS IPLVGVEPGI KPAAAVSATG VAGVLATQST LNSPRFQALL DRYGAGRRFI CQPGHGLVEA IERGDTNSPA LRALLERYLQ PMIDAGADTL VLGCTHYPFF TAAIRDLVGD RLTIVDTSDA IARQLARVLD ERGLRAPAGA PAAPPRFCST SDGVQLRALA STLLGLDVPV ETVTIASPNA RERATV

NT Sequence

ATGACGACGA ACCGTTCCAC CGCTTCCGCA GCCCGCCCCA CCGCGCCGAT CGGCATCTTC GATTCGGGGC TCGGCGGGCT GTCGGTGCTG CGCGCCGTGC GCGCGCAGCT GCCCGGCGAA TCGTTCGTCT ACGTCGCCGA CTCGCGCCAT GCGCCGTACG GCCCGCGCGA CGAGGCGTTC ATCACGGAGC GCACGCTGGC GATCTGCGAA TGGCTCGCGC GCGACGGAGC GAAAGCGCTC GTCGTCGCAT GCAACACGGC CACGGCACGC GCGATCGCGG CCGTCCGCGA ACGGCTGTCG ATCCCGCTCG TCGGCGTCGA GCCGGGCATC AAGCCGGCCG CCGCGGTGTC CGCGACCGGC GTCGCCGGCG TGCTTGCCAC CCAATCGACG CTGAACAGCC CGCGCTTTCA GGCGCTGCTC GATCGCTACG GCGCCGGCCG ACGCTTCATC TGCCAGCCGG GCCACGGGCT CGTCGAGGCG ATCGAGCGCG GCGACACGAA CTCCCCCGCC TTGCGCGCGT TGCTCGAACG CTACCTGCAA CCGATGATCG ACGCGGGCGC CGACACGCTC GTGCTCGGCT GCACGCATTA CCCGTTCTTC ACGGCGGCGA TTCGCGACCT CGTCGGCGAT CGCCTGACGA TCGTCGACAC GAGCGACGCG ATCGCGCGGC AACTCGCCCG CGTGCTCGAC GAGCGCGGGC TGCGCGCGCC GGCCGGCGCG CCTGCCGCGC CGCCGCGTTT CTGCTCGACC AGCGACGGCG TGCAATTGCG CGCACTGGCG TCGACGCTGC TTGGCCTCGA CGTGCCCGTC GAGACCGTCA CCATTGCGTC ACCGAACGCG CGCGAGCGCG CGACCGTT

Details for BuviA.00158.a.B1.GE39172

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	94

Validated AA Sequence

MTXXRSTASA ARPTAPIGIF XXGLGGLSVL RAVRAQLPGE SFVYVADSRH APYGPRDEAF ITERTLAICE WLARDGAKAL VVACNTATAR AIAAVRERLS IPLVGVEPGI KPAAAVSATG VAGVLATQST LNSPRFQALL DRYGAGRRFI CQPGHGLVEA IERGDTNSPA LRALLERYLQ PMIDAGADTL VLGCTHYPFF TAAIRDLVGD RLTIVDTSDA IARQLARVLD ERGLRAPAGA PAAPPRFCST SDGVQLRALA STLLGLDVPV ETVTIASP

Validated NT Sequence

tcggtgacgc aatggtgacg gtctcgacgg gcacgtcgag gccaagcagc gtcgacgcca gtgcgcgcaa ttgcacgccg tcgctggtcg agcagaaacg cggcggcgcg gcaggcgcgc cggccggcgc gcgcagcccg cgctcgtcga gcacgcgggc gagttgccgc gcgatcgcgt cgctcgtgtc gacgatcgtc aggcgatcgc cgacgaggtc gcgaatcgcc gccgtgaaga acgggtaatg cgtgcagccg agcacgagcg tgtcggcgcc cgcgtcgatc atcggttgca ggtagcgttc gagcaacgcg cgcaaggcgg gggagttcgt gtcgccgcgc tcgatcgcct cgacgagccc gtggcccggc tggcagatga agcgtcggcc ggcgccgtag cgatcgagca gcgcctgaaa gcgcgggctg ttcagcgtcg attgggtggc aagcacgccg gcgacgccgg tcgcggacac cgcggcggcc ggcttgatgc ccggctcgac gccgacgagc gggatcgaca gccgttcgcg gacggccgcg atcgcgcgtg ccgtggccgt gttgcatgcg acgacgagcg ctttcgctcc gtcgcgcgcg agccattcgc agatcgccag cgtgcgctcc gtgatgaacg cctcgtcgcg cgggccgtac ggcgcatggc gcgagtcggc gacgtagacg aacgattcgc cgggcagctg cgcgcgcacg gcgcgcagca ccgacagccc gccgagcccc gnancgaaga tgccgatcgg cgcggtgggg cgggctgcgg aagcggtgga acggtnnntc gtcatatggt ggtggtggtn nngagnc

Expected Protein Sequence

MAHHHHHHMT TNRSTASAAR PTAPIGIFDS GLGGLSVLRA VRAQLPGESF VYVADSRHAP YGPRDEAFIT ERTLAICEWL ARDGAKALVV ACNTATARAI AAVRERLSIP LVGVEPGIKP AAAVSATGVA GVLATQSTLN SPRFQALLDR YGAGRRFICQ PGHGLVEAIE RGDTNSPALR ALLERYLQPM IDAGADTLVL GCTHYPFFTA AIRDLVGDRL TIVDTSDAIA RQLARVLDER GLRAPAGAPA APPRFCSTSD GVQLRALAST LLGLDVPVET VTIASPNARE RATV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cgaaccgttc caccgcttcc gcagcccgcc ccaccgcgcc gatcggcatc ttcgattcgg ggctcggcgg gctgtcggtg ctgcgcgccg tgcgcgcgca gctgcccggc gaatcgttcg tctacgtcgc cgactcgcgc catgcgccgt acggcccgcg cgacgaggcg ttcatcacgg agcgcacgct ggcgatctgc gaatggctcg cgcgcgacgg agcgaaagcg ctcgtcgtcg catgcaacac ggccacggca cgcgcgatcg cggccgtccg cgaacggctg tcgatcccgc tcgtcggcgt cgagccgggc atcaagccgg ccgccgcggt gtccgcgacc ggcgtcgccg gcgtgcttgc cacccaatcg acgctgaaca gcccgcgctt tcaggcgctg ctcgatcgct acggcgccgg ccgacgcttc atctgccagc cgggccacgg gctcgtcgag gcgatcgagc gcggcgacac gaactccccc gccttgcgcg cgttgctcga acgctacctg caaccgatga tcgacgcggg cgccgacacg ctcgtgctcg gctgcacgca ttacccgttc ttcacggcgg cgattcgcga cctcgtcggc gatcgcctga cgatcgtcga cacgagcgac gcgatcgcgc ggcaactcgc ccgcgtgctc gacgagcgcg ggctgcgcgc gccggccggc gcgcctgccg cgccgccgcg tttctgctcg accagcgacg gcgtgcaatt gcgcgcactg gcgtcgacgc tgcttggcct cgacgtgccc gtcgagaccg tcaccattgc gtcaccgaac gcgcgcgagc gcgcgaccgt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BuviA.00158.a.B1.PW37799

PURIFICATION DATe:	10/13/2015
CONCENTRATION:	39mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA CGAACCGTTC CACCGCTTCC GCAGCCCGCC CCACCGCGCC GATCGGCATC TTCGATTCGG GGCTCGGCGG GCTGTCGGTG CTGCGCGCCG TGCGCGCGCA GCTGCCCGGC GAATCGTTCG TCTACGTCGC CGACTCGCGC CATGCGCCGT ACGGCCCGCG CGACGAGGCG TTCATCACGG AGCGCACGCT GGCGATCTGC GAATGGCTCG CGCGCGACGG AGCGAAAGCG CTCGTCGTCG CATGCAACAC GGCCACGGCA CGCGCGATCG CGGCCGTCCG CGAACGGCTG TCGATCCCGC TCGTCGGCGT CGAGCCGGGC ATCAAGCCGG CCGCCGCGGT GTCCGCGACC GGCGTCGCCG GCGTGCTTGC CACCCAATCG ACGCTGAACA GCCCGCGCTT TCAGGCGCTG CTCGATCGCT ACGGCGCCGG CCGACGCTTC ATCTGCCAGC CGGGCCACGG GCTCGTCGAG GCGATCGAGC GCGGCGACAC GAACTCCCCC GCCTTGCGCG CGTTGCTCGA ACGCTACCTG CAACCGATGA TCGACGCGGG CGCCGACACG CTCGTGCTCG GCTGCACGCA TTACCCGTTC TTCACGGCGG CGATTCGCGA CCTCGTCGGC GATCGCCTGA CGATCGTCGA CACGAGCGAC GCGATCGCGC GGCAACTCGC CCGCGTGCTC GACGAGCGCG GGCTGCGCGC GCCGGCCGGC GCGCCTGCCG CGCCGCCGCG TTTCTGCTCG ACCAGCGACG GCGTGCAATT GCGCGCACTG GCGTCGACGC TGCTTGGCCT CGACGTGCCC GTCGAGACCG TCACCATTGC GTCACCGAAC GCGCGCGAGC GCGCGACCGT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT