BuviA.00156.a

Threonyl-tRNA synthetase

CENTER ID: BuviA.00156.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00156.a.B1.GE39171 Full length( BuviA.00156.a ) 1 402
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00156.a.B1.PW37794 Full length( BuviA.00156.a ) 1 402

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2581
RefSeq: YP_001116313.1
UniProt: A4JKP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDFDHRVLG NKLDLFHQQD ESPGGIFWHP RGMVLYRVIE EYIRVRMRQA GFSEVRTPQI LSRDLWERSG HWEKFGPNMF SLESDRNPYC LKPMSCPCHV QIFKKGSRSY RDLPIRYCEF GAVHRAEPSG ALHGLMRARA FTQDDAHVLC LPDQIEDEVA RFCKLLKAIY ADFGFGECDV ALSTRPPVRA GDDAVWDRSE AMLASAARAA GLEFAILAGE GAFYGPKLEF HLVDRQGRKW QCGTIQLDFV LPERLGASYV AEDGAKATPV MLHHAVLGSL ERFIGILLEH YEGWLPTWLA PDQIVVANIG DDHRAFADRA ASAFEDIGCR VLRDFRAERL PRKIFDATEL AAPIVAIAGR KEALAGEVSL RMRGGEQHVM TIDAALAFLK QHARVPSATR AC
NT Sequence
ATGGACGATT TCGATCACCG AGTGTTAGGC AACAAACTGG ATCTGTTTCA TCAGCAAGAT GAAAGTCCAG GCGGCATCTT TTGGCATCCG CGCGGGATGG TTCTTTATCG CGTCATCGAG GAATACATTC GGGTCCGAAT GCGGCAGGCC GGTTTTTCCG AAGTGCGGAC GCCGCAGATC CTGTCTCGCG ATCTGTGGGA ACGGAGCGGC CATTGGGAAA AGTTCGGGCC GAACATGTTT TCGCTCGAGA GCGACCGTAA TCCGTACTGT CTCAAGCCGA TGAGTTGCCC ATGCCACGTG CAGATATTCA AGAAGGGCAG CCGTTCATAC CGCGATTTAC CGATCCGATA TTGCGAATTC GGCGCGGTCC ATCGCGCCGA ACCGTCCGGT GCATTGCACG GCCTCATGCG CGCGCGTGCA TTTACGCAGG ACGACGCTCA TGTACTTTGC CTGCCCGACC AGATCGAAGA TGAAGTCGCG CGGTTCTGCA AGCTGCTGAA GGCGATCTAC GCAGACTTCG GGTTCGGGGA GTGCGACGTG GCGTTGTCGA CGCGGCCGCC GGTGCGTGCC GGCGACGATG CGGTCTGGGA TCGCTCGGAA GCGATGCTGG CATCGGCCGC GCGGGCGGCC GGGCTGGAAT TCGCGATCCT GGCCGGAGAA GGCGCGTTCT ACGGCCCGAA GCTCGAATTT CATCTGGTCG ACCGTCAAGG ACGGAAGTGG CAGTGCGGGA CGATCCAGCT CGATTTCGTG TTGCCGGAGC GGCTCGGTGC GTCGTATGTC GCCGAGGACG GCGCGAAAGC GACGCCCGTC ATGTTGCACC ACGCCGTGCT GGGAAGCCTC GAACGGTTCA TCGGCATTCT GCTCGAACAC TACGAGGGAT GGCTGCCGAC ATGGCTGGCA CCCGACCAGA TCGTCGTCGC GAACATCGGC GACGATCATC GCGCATTTGC CGATCGCGCG GCATCCGCTT TCGAAGACAT CGGCTGCCGC GTGCTGCGCG ATTTCCGCGC CGAGCGGCTG CCCCGCAAGA TCTTCGATGC AACGGAACTC GCCGCGCCGA TCGTCGCGAT TGCCGGCCGC AAGGAAGCGC TGGCCGGCGA AGTGTCGCTG CGAATGAGGG GCGGCGAGCA GCACGTGATG ACGATCGACG CTGCGCTGGC GTTTCTCAAG CAGCATGCGC GCGTTCCATC CGCGACGCGG GCGTGC
Details for BuviA.00156.a.B1.GE39171
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 46kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHXXXHHMD DFDHRVLGNK LDLFHQQDES PGGIFWHPRG MVLYRVIEEY IRVRMRQAGF SEVRTPQILS RDLWERSGHW EKFGPNMFSL ESDRNPYCLK PMSCPCHVQI FKKGSRSYRD LPIRYCEFGA VHRAEPSGAL HGLMRARAFT QDDAHVLCLP DQIEDEVARF CKLLKAIYAD FGFGECDVAL STRPPVRAGD DAVWDRSEAM LASAARAAGL EFAILAGEGA FYGPKLEFHL VDRQGRKWQC GTIQLDFVLP ERLGASYVAE DGAKATPVML HHAVLGSLER FIGILLEHYE GWLPTWLAPD QIVVANIGDD HRAFADRAAS AFEDIGCRVL RDFRAERLPR KIFDATELAA PIVAIAGRKE ALAGEVSLRM RGGEQHVMTI DAALAFLKQH A
Validated NT Sequence
cgcgcatgct gcttgagaaa cgccagcgca gcgtcgatcg tcatcacgtg ctgctcgccg cccctcattc gcagcgacac ttcgccggcc agcgcttcct tgcggccggc aatcgcgacg atcggcgcgg cgagttccgt tgcatcgaag atcttgcggg gcagccgctc ggcgcggaaa tcgcgcagca cgcggcagcc gatgtcttcg aaagcggatg ccgcgcgatc ggcaaatgcg cgatgatcgt cgccgatgtt cgcgacgacg atctggtcgg gtgccagcca tgtcggcagc catccctcgt agtgttcgag cagaatgccg atgaaccgtt cgaggcttcc cagcacggcg tggtgcaaca tgacgggcgt cgctttcgcg ccgtcctcgg cgacatacga cgcaccgagc cgctccggca acacgaaatc gagctggatc gtcccgcact gccacttccg tccttgacgg tcgaccagat gaaattcgag cttcgggccg tagaacgcgc cttctccggc caggatcgcg aattccagcc cggccgcccg cgcggccgat gccagcatcg cttccgagcg atcccagacc gcatcgtcgc cggcacgcac cggcggccgc gtcgacaacg ccacgtcgca ctccccgaac ccgaagtctg cgtagatcgc cttcagcagc ttgcagaacc gcgcgacttc atcttcgatc tggtcgggca ggcaaagtac atgagcgtcg tcctgcgtaa atgcacgcgc gcgcatgagg ccgtgcaatg caccggacgg ttcggcgcga tggaccgcgc cgaattcgca atatcggatc ggtaaatcgc ggtatgaacg gctgcccttc ttgaatatct gcacgtggca tgggcaactc atcggcttga gacagtacgg attacggtcg ctctcgagcg aaaacatgtt cggcccgaac ttttcccaat ggccgctccg ttcccacaga tcgcgagaca ggatctgcgg cgtccgcact tcggaaaaac cggcctgccg cattcggacc cgaatgtatt cctcgatgac gcgataaaga accatcccgc gcggatgcca aaagatgccg cctggacttt catcttgctg atgaaacaga tccagtttgt tgcctaacac tcggtgatcg aaatcgtcca tatggtggtn nnnntngtga gccat
Expected Protein Sequence
MAHHHHHHMD DFDHRVLGNK LDLFHQQDES PGGIFWHPRG MVLYRVIEEY IRVRMRQAGF SEVRTPQILS RDLWERSGHW EKFGPNMFSL ESDRNPYCLK PMSCPCHVQI FKKGSRSYRD LPIRYCEFGA VHRAEPSGAL HGLMRARAFT QDDAHVLCLP DQIEDEVARF CKLLKAIYAD FGFGECDVAL STRPPVRAGD DAVWDRSEAM LASAARAAGL EFAILAGEGA FYGPKLEFHL VDRQGRKWQC GTIQLDFVLP ERLGASYVAE DGAKATPVML HHAVLGSLER FIGILLEHYE GWLPTWLAPD QIVVANIGDD HRAFADRAAS AFEDIGCRVL RDFRAERLPR KIFDATELAA PIVAIAGRKE ALAGEVSLRM RGGEQHVMTI DAALAFLKQH ARVPSATRAC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg atttcgatca ccgagtgtta ggcaacaaac tggatctgtt tcatcagcaa gatgaaagtc caggcggcat cttttggcat ccgcgcggga tggttcttta tcgcgtcatc gaggaataca ttcgggtccg aatgcggcag gccggttttt ccgaagtgcg gacgccgcag atcctgtctc gcgatctgtg ggaacggagc ggccattggg aaaagttcgg gccgaacatg ttttcgctcg agagcgaccg taatccgtac tgtctcaagc cgatgagttg cccatgccac gtgcagatat tcaagaaggg cagccgttca taccgcgatt taccgatccg atattgcgaa ttcggcgcgg tccatcgcgc cgaaccgtcc ggtgcattgc acggcctcat gcgcgcgcgt gcatttacgc aggacgacgc tcatgtactt tgcctgcccg accagatcga agatgaagtc gcgcggttct gcaagctgct gaaggcgatc tacgcagact tcgggttcgg ggagtgcgac gtggcgttgt cgacgcggcc gccggtgcgt gccggcgacg atgcggtctg ggatcgctcg gaagcgatgc tggcatcggc cgcgcgggcg gccgggctgg aattcgcgat cctggccgga gaaggcgcgt tctacggccc gaagctcgaa tttcatctgg tcgaccgtca aggacggaag tggcagtgcg ggacgatcca gctcgatttc gtgttgccgg agcggctcgg tgcgtcgtat gtcgccgagg acggcgcgaa agcgacgccc gtcatgttgc accacgccgt gctgggaagc ctcgaacggt tcatcggcat tctgctcgaa cactacgagg gatggctgcc gacatggctg gcacccgacc agatcgtcgt cgcgaacatc ggcgacgatc atcgcgcatt tgccgatcgc gcggcatccg ctttcgaaga catcggctgc cgcgtgctgc gcgatttccg cgccgagcgg ctgccccgca agatcttcga tgcaacggaa ctcgccgcgc cgatcgtcgc gattgccggc cgcaaggaag cgctggccgg cgaagtgtcg ctgcgaatga ggggcggcga gcagcacgtg atgacgatcg acgctgcgct ggcgtttctc aagcagcatg cgcgcgttcc atccgcgacg cgggcgtgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuviA.00156.a.B1.PW37794
PURIFICATION DATe: 10/5/2015
CONCENTRATION: 5mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHXXXHHMD DFDHRVLGNK LDLFHQQDES PGGIFWHPRG MVLYRVIEEY IRVRMRQAGF SEVRTPQILS RDLWERSGHW EKFGPNMFSL ESDRNPYCLK PMSCPCHVQI FKKGSRSYRD LPIRYCEFGA VHRAEPSGAL HGLMRARAFT QDDAHVLCLP DQIEDEVARF CKLLKAIYAD FGFGECDVAL STRPPVRAGD DAVWDRSEAM LASAARAAGL EFAILAGEGA FYGPKLEFHL VDRQGRKWQC GTIQLDFVLP ERLGASYVAE DGAKATPVML HHAVLGSLER FIGILLEHYE GWLPTWLAPD QIVVANIGDD HRAFADRAAS AFEDIGCRVL RDFRAERLPR KIFDATELAA PIVAIAGRKE ALAGEVSLRM RGGEQHVMTI DAALAFLKQH A
Validated NT Sequence
cgcgcatgct gcttgagaaa cgccagcgca gcgtcgatcg tcatcacgtg ctgctcgccg cccctcattc gcagcgacac ttcgccggcc agcgcttcct tgcggccggc aatcgcgacg atcggcgcgg cgagttccgt tgcatcgaag atcttgcggg gcagccgctc ggcgcggaaa tcgcgcagca cgcggcagcc gatgtcttcg aaagcggatg ccgcgcgatc ggcaaatgcg cgatgatcgt cgccgatgtt cgcgacgacg atctggtcgg gtgccagcca tgtcggcagc catccctcgt agtgttcgag cagaatgccg atgaaccgtt cgaggcttcc cagcacggcg tggtgcaaca tgacgggcgt cgctttcgcg ccgtcctcgg cgacatacga cgcaccgagc cgctccggca acacgaaatc gagctggatc gtcccgcact gccacttccg tccttgacgg tcgaccagat gaaattcgag cttcgggccg tagaacgcgc cttctccggc caggatcgcg aattccagcc cggccgcccg cgcggccgat gccagcatcg cttccgagcg atcccagacc gcatcgtcgc cggcacgcac cggcggccgc gtcgacaacg ccacgtcgca ctccccgaac ccgaagtctg cgtagatcgc cttcagcagc ttgcagaacc gcgcgacttc atcttcgatc tggtcgggca ggcaaagtac atgagcgtcg tcctgcgtaa atgcacgcgc gcgcatgagg ccgtgcaatg caccggacgg ttcggcgcga tggaccgcgc cgaattcgca atatcggatc ggtaaatcgc ggtatgaacg gctgcccttc ttgaatatct gcacgtggca tgggcaactc atcggcttga gacagtacgg attacggtcg ctctcgagcg aaaacatgtt cggcccgaac ttttcccaat ggccgctccg ttcccacaga tcgcgagaca ggatctgcgg cgtccgcact tcggaaaaac cggcctgccg cattcggacc cgaatgtatt cctcgatgac gcgataaaga accatcccgc gcggatgcca aaagatgccg cctggacttt catcttgctg atgaaacaga tccagtttgt tgcctaacac tcggtgatcg aaatcgtcca tatggtggtn nnnntngtga gccat
Expressed Protein Sequence
MAHHHHHHMD DFDHRVLGNK LDLFHQQDES PGGIFWHPRG MVLYRVIEEY IRVRMRQAGF SEVRTPQILS RDLWERSGHW EKFGPNMFSL ESDRNPYCLK PMSCPCHVQI FKKGSRSYRD LPIRYCEFGA VHRAEPSGAL HGLMRARAFT QDDAHVLCLP DQIEDEVARF CKLLKAIYAD FGFGECDVAL STRPPVRAGD DAVWDRSEAM LASAARAAGL EFAILAGEGA FYGPKLEFHL VDRQGRKWQC GTIQLDFVLP ERLGASYVAE DGAKATPVML HHAVLGSLER FIGILLEHYE GWLPTWLAPD QIVVANIGDD HRAFADRAAS AFEDIGCRVL RDFRAERLPR KIFDATELAA PIVAIAGRKE ALAGEVSLRM RGGEQHVMTI DAALAFLKQH ARVPSATRAC
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACG ATTTCGATCA CCGAGTGTTA GGCAACAAAC TGGATCTGTT TCATCAGCAA GATGAAAGTC CAGGCGGCAT CTTTTGGCAT CCGCGCGGGA TGGTTCTTTA TCGCGTCATC GAGGAATACA TTCGGGTCCG AATGCGGCAG GCCGGTTTTT CCGAAGTGCG GACGCCGCAG ATCCTGTCTC GCGATCTGTG GGAACGGAGC GGCCATTGGG AAAAGTTCGG GCCGAACATG TTTTCGCTCG AGAGCGACCG TAATCCGTAC TGTCTCAAGC CGATGAGTTG CCCATGCCAC GTGCAGATAT TCAAGAAGGG CAGCCGTTCA TACCGCGATT TACCGATCCG ATATTGCGAA TTCGGCGCGG TCCATCGCGC CGAACCGTCC GGTGCATTGC ACGGCCTCAT GCGCGCGCGT GCATTTACGC AGGACGACGC TCATGTACTT TGCCTGCCCG ACCAGATCGA AGATGAAGTC GCGCGGTTCT GCAAGCTGCT GAAGGCGATC TACGCAGACT TCGGGTTCGG GGAGTGCGAC GTGGCGTTGT CGACGCGGCC GCCGGTGCGT GCCGGCGACG ATGCGGTCTG GGATCGCTCG GAAGCGATGC TGGCATCGGC CGCGCGGGCG GCCGGGCTGG AATTCGCGAT CCTGGCCGGA GAAGGCGCGT TCTACGGCCC GAAGCTCGAA TTTCATCTGG TCGACCGTCA AGGACGGAAG TGGCAGTGCG GGACGATCCA GCTCGATTTC GTGTTGCCGG AGCGGCTCGG TGCGTCGTAT GTCGCCGAGG ACGGCGCGAA AGCGACGCCC GTCATGTTGC ACCACGCCGT GCTGGGAAGC CTCGAACGGT TCATCGGCAT TCTGCTCGAA CACTACGAGG GATGGCTGCC GACATGGCTG GCACCCGACC AGATCGTCGT CGCGAACATC GGCGACGATC ATCGCGCATT TGCCGATCGC GCGGCATCCG CTTTCGAAGA CATCGGCTGC CGCGTGCTGC GCGATTTCCG CGCCGAGCGG CTGCCCCGCA AGATCTTCGA TGCAACGGAA CTCGCCGCGC CGATCGTCGC GATTGCCGGC CGCAAGGAAG CGCTGGCCGG CGAAGTGTCG CTGCGAATGA GGGGCGGCGA GCAGCACGTG ATGACGATCG ACGCTGCGCT GGCGTTTCTC AAGCAGCATG CGCGCGTTCC ATCCGCGACG CGGGCGTGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT