SSGCID: Target Page

BuviA.00153.a

DNA primase

CENTER ID:	BuviA.00153.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00153.a.B1.GE35362	Full length( BuviA.00153.a )		1	426

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00153.a.B1.PS01569	Full length( BuviA.00153.a )		1	426

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.425
RefSeq:	YP_001110054.1
UniProt:	A4JW69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKYIRFSDE FLERIRGETD LPALIERDVK LKRAGQDFVA CCPFHEEKTP SFRVSADKNT YRCWGCGARG DAIEWMRNKH HLSFQDAVVH LAGVSGIPLP VEERDSDEDK AQRKRLAGLY QALKEAARVF SRGLAKSPAA RAYLEQKRGL SAETIERFGL GVVAKGVVEL LGGSTSREAL LGSGLAAQRD DGEIYDRFRH RIMFPIHNES GNLVGFAGRS MIEKPDKTPK YVNCPETEIF HKGRELYALH LAKPAIRSGR VAVIVEGYFD VISLHQAGDE RAVAPMGTAL TNLQVRRLLV HADTIVFAFD GDAAGRKAAL GAAAVLLDEM KDGKTARFLF LPEGEDPDSF VRAHGLEAWH AATEQAAPLS AFLADYVAHG LDRDVPESQV KAAEKAKAIL ARIQHAELFG RAMKAKFEEV VGVALD

NT Sequence

ATGGCCAAGT ACATCCGGTT CAGCGACGAA TTTCTGGAGC GCATCCGAGG CGAAACCGAT CTTCCGGCGC TGATCGAGCG CGACGTGAAA CTCAAGCGCG CCGGCCAGGA CTTTGTCGCA TGCTGCCCGT TCCACGAAGA GAAGACGCCT TCGTTCCGTG TCTCGGCCGA CAAGAACACG TATCGGTGTT GGGGATGCGG CGCGCGGGGC GACGCGATCG AGTGGATGCG CAACAAGCAT CACCTGTCGT TCCAGGACGC GGTGGTTCAT CTAGCGGGCG TGAGCGGCAT TCCGCTGCCC GTCGAGGAGC GCGATAGCGA CGAAGACAAA GCGCAGCGCA AGCGGCTGGC CGGGCTTTAC CAGGCGCTGA AAGAGGCCGC GCGCGTCTTC TCTCGGGGCT TGGCTAAGAG CCCCGCCGCT CGCGCGTACC TCGAGCAAAA GCGCGGCCTG AGCGCTGAGA CGATCGAGCG ATTCGGGCTC GGCGTCGTCG CGAAAGGCGT TGTCGAGCTG CTCGGCGGCT CGACGAGCCG CGAAGCCCTG CTGGGCTCCG GGCTTGCCGC GCAACGCGAT GATGGCGAGA TCTACGATCG ATTCCGCCAT CGCATCATGT TCCCGATCCA CAACGAGAGC GGCAACCTGG TTGGTTTCGC CGGCCGTTCG ATGATCGAGA AGCCGGACAA GACACCGAAG TACGTGAATT GCCCGGAGAC AGAGATCTTT CACAAGGGCC GGGAGTTGTA CGCGCTGCAT CTGGCCAAGC CAGCGATTCG CAGCGGGCGC GTCGCGGTGA TTGTCGAAGG GTATTTCGAC GTCATCAGTC TGCACCAGGC AGGCGATGAG CGCGCCGTCG CGCCGATGGG CACGGCGCTG ACGAACCTCC AGGTACGGCG CCTACTGGTG CATGCCGACA CGATCGTCTT TGCGTTCGAT GGCGACGCGG CCGGCCGCAA AGCGGCGCTC GGCGCCGCTG CGGTCCTGCT CGACGAGATG AAGGACGGAA AAACGGCGCG CTTCCTGTTT CTGCCGGAAG GCGAAGATCC CGATAGCTTC GTGCGTGCTC ACGGGCTGGA AGCGTGGCAT GCGGCGACGG AACAGGCCGC GCCACTGAGC GCGTTTCTGG CCGATTACGT GGCGCACGGG CTCGATAGGG ACGTGCCTGA AAGCCAGGTC AAGGCCGCCG AAAAAGCAAA GGCAATCCTC GCTCGCATCC AGCATGCCGA GTTGTTCGGG CGTGCGATGA AGGCGAAATT CGAGGAAGTC GTCGGCGTGG CGTTGGAT

Details for BuviA.00153.a.B1.GE35362

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	78

Validated AA Sequence

MRNKHHLSFQ DAVVHLAGVS GIPLPVEERD SDEDKAQRKR LAGLYQALKE AARVFSRGLA KSPAARAYLE QKRGLSAETI ERFGLGVVAK GVVELLGGST SREALLGSGL AAQRDDGEIY DRFRHRIMFP IHNESGNLVG FAGRSMIEKP DKTPKYVNCP ETEIFHKGRE LYALHLAKPA IRSGRVAVIV EGYFDVISLH QAGDERAVAP MGTALTNLQV RRLLVHADTI VFAFDGDAAG RKAALGAAAV LLDEMKDGKT ARFLFLPEGE DPDSFVRAHG LEAWHAATEQ AAPLSAFLAD YVAHGLDRDV PESQVKAAEK AKAILARIQH AELFGRAMKA KF

Validated NT Sequence

cgaatttcgc cttcatcgca cgcccgaaca actcggcatg ctggatgcga gcgaggattg cctttgcttt ttcggcggcc ttgacctggc tttcaggcac gtccctatcg agcccgtgcg ccacgtaatc ggccagaaac gcgctcagtg gcgcggcctg ttccgtcgcc gcatgccacg cttccagccc gtgagcacgc acgaagctat cgggatcttc gccttccggc agaaacagga agcgcgccgt ttttccgtcc ttcatctcgt cgagcaggac cgcagcggcg ccgagcgccg ctttgcggcc ggccgcgtcg ccatcgaacg caaagacgat cgtgtcggca tgcaccagta ggcgccgtac ctggaggttc gtcagcgccg tgcccatcgg cgcgacggcg cgctcatcgc ctgcctggtg cagactgatg acgtcgaaat acccttcgac aatcaccgcg acgcgcccgc tgcgaatcgc tggcttggcc agatgcagcg cgtacaactc ccggcccttg tgaaagatct ctgtctccgg gcaattcacg tacttcggtg tcttgtccgg cttctcgatc atcgaacggc cggcgaaacc aaccaggttg ccgctctcgt tgtggatcgg gaacatgatg cgatggcgga atcgatcgta gatctcgcca tcatcgcgtt gcgcggcaag cccggagccc agcagggctt cgcggctcgt cgagccgccg agcagctcga caacgccttt cgcgacgacg ccgagcccga atcgctcgat cgtctcagcg ctcaggccgc gcttttgctc gaggtacgcg cgagcggcgg ggctcttagc caagccccga gagaagacgc gcgcggcctc tttcagcgcc tggtaaagcc cggccagccg cttgcgctgc gctttgtctt cgtcgctatc gcgctcctcg acgggcagcg gaatgccgct cacgcccgct agatgaacca ccgcgtcctg gaacgacagg tgatgcttgt tgcgcatcca ctcgatcgcg tcgccccgcg cgccgcatcc ccaacaccga tacgtgttct tgtcggccga gacacggaac gaaggcgtct tctcttcgtg gaacgggcag catgcgacaa agtcctggcc ggcgcgcttg agtttcacgt cgcgctcgat cagcgccgga agatcggttt cgcctcggat gcgctccaga aattcgtcgc tgaaccggat gtact

Expected Protein Sequence

MAHHHHHHMA KYIRFSDEFL ERIRGETDLP ALIERDVKLK RAGQDFVACC PFHEEKTPSF RVSADKNTYR CWGCGARGDA IEWMRNKHHL SFQDAVVHLA GVSGIPLPVE ERDSDEDKAQ RKRLAGLYQA LKEAARVFSR GLAKSPAARA YLEQKRGLSA ETIERFGLGV VAKGVVELLG GSTSREALLG SGLAAQRDDG EIYDRFRHRI MFPIHNESGN LVGFAGRSMI EKPDKTPKYV NCPETEIFHK GRELYALHLA KPAIRSGRVA VIVEGYFDVI SLHQAGDERA VAPMGTALTN LQVRRLLVHA DTIVFAFDGD AAGRKAALGA AAVLLDEMKD GKTARFLFLP EGEDPDSFVR AHGLEAWHAA TEQAAPLSAF LADYVAHGLD RDVPESQVKA AEKAKAILAR IQHAELFGRA MKAKFEEVVG VALD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca agtacatccg gttcagcgac gaatttctgg agcgcatccg aggcgaaacc gatcttccgg cgctgatcga gcgcgacgtg aaactcaagc gcgccggcca ggactttgtc gcatgctgcc cgttccacga agagaagacg ccttcgttcc gtgtctcggc cgacaagaac acgtatcggt gttggggatg cggcgcgcgg ggcgacgcga tcgagtggat gcgcaacaag catcacctgt cgttccagga cgcggtggtt catctagcgg gcgtgagcgg cattccgctg cccgtcgagg agcgcgatag cgacgaagac aaagcgcagc gcaagcggct ggccgggctt taccaggcgc tgaaagaggc cgcgcgcgtc ttctctcggg gcttggctaa gagccccgcc gctcgcgcgt acctcgagca aaagcgcggc ctgagcgctg agacgatcga gcgattcggg ctcggcgtcg tcgcgaaagg cgttgtcgag ctgctcggcg gctcgacgag ccgcgaagcc ctgctgggct ccgggcttgc cgcgcaacgc gatgatggcg agatctacga tcgattccgc catcgcatca tgttcccgat ccacaacgag agcggcaacc tggttggttt cgccggccgt tcgatgatcg agaagccgga caagacaccg aagtacgtga attgcccgga gacagagatc tttcacaagg gccgggagtt gtacgcgctg catctggcca agccagcgat tcgcagcggg cgcgtcgcgg tgattgtcga agggtatttc gacgtcatca gtctgcacca ggcaggcgat gagcgcgccg tcgcgccgat gggcacggcg ctgacgaacc tccaggtacg gcgcctactg gtgcatgccg acacgatcgt ctttgcgttc gatggcgacg cggccggccg caaagcggcg ctcggcgccg ctgcggtcct gctcgacgag atgaaggacg gaaaaacggc gcgcttcctg tttctgccgg aaggcgaaga tcccgatagc ttcgtgcgtg ctcacgggct ggaagcgtgg catgcggcga cggaacaggc cgcgccactg agcgcgtttc tggccgatta cgtggcgcac gggctcgata gggacgtgcc tgaaagccag gtcaaggccg ccgaaaaagc aaaggcaatc ctcgctcgca tccagcatgc cgagttgttc gggcgtgcga tgaaggcgaa attcgaggaa gtcgtcggcg tggcgttgga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BuviA.00153.a.B1.PS01569

PURIFICATION DATe:	7/26/2012
CONCENTRATION:	34.28mg/ml
OBSERVED MW:	46kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	78

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA AGTACATCCG GTTCAGCGAC GAATTTCTGG AGCGCATCCG AGGCGAAACC GATCTTCCGG CGCTGATCGA GCGCGACGTG AAACTCAAGC GCGCCGGCCA GGACTTTGTC GCATGCTGCC CGTTCCACGA AGAGAAGACG CCTTCGTTCC GTGTCTCGGC CGACAAGAAC ACGTATCGGT GTTGGGGATG CGGCGCGCGG GGCGACGCGA TCGAGTGGAT GCGCAACAAG CATCACCTGT CGTTCCAGGA CGCGGTGGTT CATCTAGCGG GCGTGAGCGG CATTCCGCTG CCCGTCGAGG AGCGCGATAG CGACGAAGAC AAAGCGCAGC GCAAGCGGCT GGCCGGGCTT TACCAGGCGC TGAAAGAGGC CGCGCGCGTC TTCTCTCGGG GCTTGGCTAA GAGCCCCGCC GCTCGCGCGT ACCTCGAGCA AAAGCGCGGC CTGAGCGCTG AGACGATCGA GCGATTCGGG CTCGGCGTCG TCGCGAAAGG CGTTGTCGAG CTGCTCGGCG GCTCGACGAG CCGCGAAGCC CTGCTGGGCT CCGGGCTTGC CGCGCAACGC GATGATGGCG AGATCTACGA TCGATTCCGC CATCGCATCA TGTTCCCGAT CCACAACGAG AGCGGCAACC TGGTTGGTTT CGCCGGCCGT TCGATGATCG AGAAGCCGGA CAAGACACCG AAGTACGTGA ATTGCCCGGA GACAGAGATC TTTCACAAGG GCCGGGAGTT GTACGCGCTG CATCTGGCCA AGCCAGCGAT TCGCAGCGGG CGCGTCGCGG TGATTGTCGA AGGGTATTTC GACGTCATCA GTCTGCACCA GGCAGGCGAT GAGCGCGCCG TCGCGCCGAT GGGCACGGCG CTGACGAACC TCCAGGTACG GCGCCTACTG GTGCATGCCG ACACGATCGT CTTTGCGTTC GATGGCGACG CGGCCGGCCG CAAAGCGGCG CTCGGCGCCG CTGCGGTCCT GCTCGACGAG ATGAAGGACG GAAAAACGGC GCGCTTCCTG TTTCTGCCGG AAGGCGAAGA TCCCGATAGC TTCGTGCGTG CTCACGGGCT GGAAGCGTGG CATGCGGCGA CGGAACAGGC CGCGCCACTG AGCGCGTTTC TGGCCGATTA CGTGGCGCAC GGGCTCGATA GGGACGTGCC TGAAAGCCAG GTCAAGGCCG CCGAAAAAGC AAAGGCAATC CTCGCTCGCA TCCAGCATGC CGAGTTGTTC GGGCGTGCGA TGAAGGCGAA ATTCGAGGAA GTCGTCGGCG TGGCGTTGGA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT