BuviA.00149.a

Cyclopropane-fatty-acyl-phospholipid synthase

CENTER ID: BuviA.00149.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00149.a.B2.GE39167 Mature protein( BuviA.00149.a ) 19 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.1292
RefSeq: YP_001115088.1
UniProt: A4JRD0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLLRLLSAA SQAALPLSAR LFIALLRRIH DGHLTLVTPE GAQHVFGDPH RQPAATLQIR DWRACRAILR AGDIGFADAY RAGWVDTPDP VALLRVAIRN QPVIAKTVTG GPFARVWYAL RHRLRANTRA GSRRNIHAHY DLGNDFYRLW LDDTWTYSSA CFDGDDRRPL ADAQAAKYQR IVATLGLRAG MRVLEIGCGW GGFAVHAARQ GVRVHGVTIS QQQYSLARER VAREGLADLV TIELRDYRDV DGQYDAAVSI EMFEAVGEKF WPVYFDTLVR RLKPGARALI QSITIAESQF EAYRASSDFI REFIFPGGML PSAERFVDAA RRAGLAAAPV HAFGDDYART LRTWRATFDA HADLVRAQGF DETFIRTWRL YLAYCEAGFA ERRTDVMHFV VSAGR
NT Sequence
ATGACCCTGC TGCGCCTGCT CTCTGCCGCC TCGCAAGCGG CGCTGCCGCT GTCGGCCCGG TTGTTCATCG CGCTGCTGCG ACGCATCCAC GACGGCCATC TCACGCTCGT GACGCCCGAA GGCGCACAAC ACGTGTTCGG CGATCCGCAC CGCCAGCCGG CCGCGACGCT GCAGATCCGC GACTGGCGCG CCTGCCGCGC GATCCTGCGC GCCGGCGACA TCGGCTTTGC CGACGCGTAT CGCGCCGGCT GGGTCGACAC GCCCGATCCG GTCGCGCTGC TGCGCGTCGC GATCCGCAAT CAGCCGGTGA TCGCCAAGAC CGTGACGGGC GGCCCCTTCG CACGCGTGTG GTACGCGCTG CGCCATCGGC TGCGCGCGAA TACGCGCGCG GGCAGCCGCC GCAACATCCA CGCGCATTAC GACCTCGGCA ACGACTTCTA CCGGTTGTGG CTCGACGATA CGTGGACCTA TTCGAGCGCA TGCTTCGACG GCGACGACCG CCGCCCGCTC GCCGACGCGC AGGCGGCGAA GTATCAGCGC ATCGTCGCGA CGCTCGGCCT GCGCGCCGGC ATGCGCGTGC TCGAGATCGG CTGCGGCTGG GGCGGCTTCG CCGTACACGC GGCGCGTCAG GGCGTCCGCG TGCACGGCGT GACGATCTCG CAGCAACAAT ATTCGCTGGC TCGCGAACGC GTTGCGCGCG AGGGGCTGGC CGATCTCGTC ACGATCGAAT TGCGCGACTA TCGCGACGTC GACGGCCAAT ACGATGCGGC GGTGTCGATC GAAATGTTCG AAGCCGTCGG CGAAAAGTTC TGGCCCGTGT ACTTCGACAC GCTCGTGCGG CGTCTGAAGC CCGGTGCACG CGCGTTGATC CAATCGATCA CGATCGCCGA GTCGCAATTC GAGGCGTACC GCGCGTCGAG CGACTTCATC CGCGAATTCA TCTTTCCCGG CGGGATGTTG CCGAGCGCCG AGCGCTTCGT CGACGCAGCG CGCCGCGCCG GACTCGCCGC CGCACCGGTG CACGCGTTCG GCGACGACTA CGCGCGCACG CTGCGCACGT GGCGGGCGAC GTTCGATGCA CACGCCGATC TGGTGCGCGC GCAAGGATTC GACGAGACCT TCATTCGCAC GTGGCGGCTG TACCTCGCCT ATTGCGAAGC TGGATTTGCC GAGCGGCGTA CCGACGTGAT GCATTTCGTC GTGTCGGCGG GCCGC
Details for BuviA.00149.a.B2.GE39167
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 45kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA RLFIALLRRI HDGHLTLVTP EGAQHVFGDP HRQPAATLQI RDWRACRAIL RAGDIGFADA YRAGWVDTPD PVALLRVAIR NQPVIAKTVT GGPFARVWYA LRHRLRANTR AGSRRNIHAH YDLGNDFYRL WLDDTWTYSS ACFDGDDRRP LADAQAAKYQ RIVATLGLRA GMRVLEIGCG WGGFAVHAAR QGVRVHGVTI SQQQYSLARE RVAREGLADL VTIELRDYRD VDGQYDAAVS IEMFEAVGEK FWPVYFDTLV RRLKPGARAL IQSITIAESQ FEAYRASSDF IREFIFPGGM LPSAERFVDA ARRAGLAAAP VHAFGDDYAR TLRTWRATFD AHADLVRAQG FDETFIRTWR LYLAYCEAGF AERRTDV
Validated NT Sequence
tnacgtcggt acgccgctcg gcaaatccag cttcgcaata ggcgaggtac agccgccacg tgcgaatgaa ggtctcgtcg aatccttgcg cgcgcaccag atcggcgtgt gcatcgaacg tcgcccgcca cgtgcgcagc gtgcgcgcgt agtcgtcgcc gaacgcgtgc accggtgcgg cggcgagtcc ggcgcggcgc gctgcgtcga cgaagcgctc ggcgctcggc aacatcccgc cgggaaagat gaattcgcgg atgaagtcgc tcgacgcgcg gtacgcctcg aattgcgact cggcgatcgt gatcgattgg atcaacgcgc gtgcaccggg cttcagacgc cgcacgagcg tgtcgaagta cacgggccag aacttttcgc cgacggcttc gaacatttcg atcgacaccg ccgcatcgta ttggccgtcg acgtcgcgat agtcgcgcaa ttcgatcgtg acgagatcgg ccagcccctc gcgcgcaacg cgttcgcgag ccagcgaata ttgttgctgc gagatcgtca cgccgtgcac gcggacgccc tgacgcgccg cgtgtacggc gaagccgccc cagccgcagc cgatctcgag cacgcgcatg ccggcgcgca ggccgagcgt cgcgacgatg cgctgatact tcgccgcctg cgcgtcggcg agcgggcggc ggtcgtcgcc gtcgaagcat gcgctcgaat aggtccacgt atcgtcgagc cacaaccggt agaagtcgtt gccgaggtcg taatgcgcgt ggatgttgcg gcggctgccc gcgcgcgtat tcgcgcgcag ccgatggcgc agcgcgtacc acacgcgtgc gaaggggccg cccgtcacgg tcttggcgat caccggctga ttgcggatcg cgacgcgcag cagcgcgacc ggatcgggcg tgtcgaccca gccggcgcga tacgcgtcgg caaagccgat gtcgccggcg cgcaggatcg cgcggcaggc gcgccagtcg cggatctgca gcgtcgcggc cggctggcgg tgcggatcgc cgaacacgtg ttgtgcgcct tcgggcgtca cgagcgtgag atggccgtcg tggatgcgtc gcagcagcgc gatgaacaac cgggccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHAR LFIALLRRIH DGHLTLVTPE GAQHVFGDPH RQPAATLQIR DWRACRAILR AGDIGFADAY RAGWVDTPDP VALLRVAIRN QPVIAKTVTG GPFARVWYAL RHRLRANTRA GSRRNIHAHY DLGNDFYRLW LDDTWTYSSA CFDGDDRRPL ADAQAAKYQR IVATLGLRAG MRVLEIGCGW GGFAVHAARQ GVRVHGVTIS QQQYSLARER VAREGLADLV TIELRDYRDV DGQYDAAVSI EMFEAVGEKF WPVYFDTLVR RLKPGARALI QSITIAESQF EAYRASSDFI REFIFPGGML PSAERFVDAA RRAGLAAAPV HAFGDDYART LRTWRATFDA HADLVRAQGF DETFIRTWRL YLAYCEAGFA ERRTDVMHFV VSAGR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcccggt tgttcatcgc gctgctgcga cgcatccacg acggccatct cacgctcgtg acgcccgaag gcgcacaaca cgtgttcggc gatccgcacc gccagccggc cgcgacgctg cagatccgcg actggcgcgc ctgccgcgcg atcctgcgcg ccggcgacat cggctttgcc gacgcgtatc gcgccggctg ggtcgacacg cccgatccgg tcgcgctgct gcgcgtcgcg atccgcaatc agccggtgat cgccaagacc gtgacgggcg gccccttcgc acgcgtgtgg tacgcgctgc gccatcggct gcgcgcgaat acgcgcgcgg gcagccgccg caacatccac gcgcattacg acctcggcaa cgacttctac cggttgtggc tcgacgatac gtggacctat tcgagcgcat gcttcgacgg cgacgaccgc cgcccgctcg ccgacgcgca ggcggcgaag tatcagcgca tcgtcgcgac gctcggcctg cgcgccggca tgcgcgtgct cgagatcggc tgcggctggg gcggcttcgc cgtacacgcg gcgcgtcagg gcgtccgcgt gcacggcgtg acgatctcgc agcaacaata ttcgctggct cgcgaacgcg ttgcgcgcga ggggctggcc gatctcgtca cgatcgaatt gcgcgactat cgcgacgtcg acggccaata cgatgcggcg gtgtcgatcg aaatgttcga agccgtcggc gaaaagttct ggcccgtgta cttcgacacg ctcgtgcggc gtctgaagcc cggtgcacgc gcgttgatcc aatcgatcac gatcgccgag tcgcaattcg aggcgtaccg cgcgtcgagc gacttcatcc gcgaattcat ctttcccggc gggatgttgc cgagcgccga gcgcttcgtc gacgcagcgc gccgcgccgg actcgccgcc gcaccggtgc acgcgttcgg cgacgactac gcgcgcacgc tgcgcacgtg gcgggcgacg ttcgatgcac acgccgatct ggtgcgcgcg caaggattcg acgagacctt cattcgcacg tggcggctgt acctcgccta ttgcgaagct ggatttgccg agcggcgtac cgacgtgatg catttcgtcg tgtcggcggg ccgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat