SSGCID: Target Page

BuviA.00148.c

Phage integrase family protein

CENTER ID:	BuviA.00148.c
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00148.c.B1.GE39166	Full length( BuviA.00148.c )		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.262
RefSeq:	YP_001109870.1
UniProt:	A4JUY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNPSTQTISP LRQRMIDDMR MRKFTAKTQN GYLRAVKRFA GFLGRSPDTA TVEDLRWYQL HLVDTGTSPI SLNAAIAGLK FFFDVTLDRA ELMAKMQPVR VPQRLPVVLS REEVGRLIAA SGNLKHQTAL SLAYGTGLRA SEVVALKVCD IDSQRMTLRV EQGKGRKDRY AMLSPVLLER LRVWWRVARA QGKMLDGGWL FPGLNPIESL STRQLNRAIH AAAAEAKIDK RVSMHTLRHC FATHLLEQKV DIRVIQVLLG HKQLDTTALY TQVATDLLRE VVSPLEMLHP E

NT Sequence

ATGAACCCAT CCACTCAAAC CATCAGTCCG CTGCGCCAGC GCATGATCGA CGACATGCGG ATGCGCAAAT TCACTGCCAA GACACAAAAC GGTTACCTGC GCGCCGTGAA ACGATTCGCG GGGTTTCTCG GCCGCTCGCC TGATACGGCC ACCGTAGAAG ATCTGAGGTG GTATCAGTTG CATCTCGTCG ATACCGGCAC TTCGCCGATC TCGCTCAATG CCGCGATTGC CGGATTGAAG TTCTTCTTCG ACGTCACGCT CGATCGTGCC GAGCTGATGG CCAAGATGCA ACCCGTTCGT GTCCCGCAGA GACTCCCCGT CGTATTAAGC CGCGAGGAAG TGGGTCGCCT GATCGCGGCG TCTGGCAACC TGAAGCATCA AACGGCACTG TCGCTCGCCT ACGGAACTGG GCTGCGCGCC AGCGAAGTTG TCGCGCTCAA GGTTTGCGAC ATTGACAGCC AACGCATGAC GCTGCGCGTT GAACAGGGCA AAGGCCGCAA GGATCGCTAC GCCATGCTTT CACCAGTACT GCTCGAACGC CTGCGAGTGT GGTGGCGTGT GGCACGCGCA CAGGGCAAGA TGCTCGATGG CGGCTGGCTG TTTCCGGGCC TCAACCCGAT CGAATCGCTC AGCACCCGCC AGCTCAACCG GGCTATCCAT GCCGCCGCCG CGGAGGCGAA GATCGACAAG CGCGTCTCGA TGCACACGCT TCGACATTGC TTTGCAACGC ACCTGCTGGA GCAGAAGGTG GATATTCGTG TGATCCAGGT GCTACTCGGG CACAAGCAAT TGGACACGAC GGCGCTCTAT ACCCAGGTCG CCACCGACCT CCTGCGCGAA GTGGTCAGCC CCCTGGAGAT GCTGCACCCG GAA

Details for BuviA.00148.c.B1.GE39166

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN PSTQTISPLR QRMIDDMRMR KFTAKTQNGY LRAVKRFAGF LGRSPDTATV EDLRWYQLHL VDTGTSPISL NAAIAGLKFF FDVTLDRAEL MAKMQPVRVP QRLPVVLSRE EVGRLIAASG NLKHQTALSL AYGTGLRASE VVALKVCDID SQRMTLRVEQ GKGRKDRYAM LSPVLLERLR VWWRVARAQG KMLDGGWLFP GLNPIESLST RQLNRAIHAA AAEAKIDKRV SMHTLRHCFA THLLEQKVDI RVIQVLLGHK QLDTTALYTQ VATDLLREVV XXXEMLHPE

Validated NT Sequence

atcttactca cttattccgg gtgcagcatc tcnnnnnngn tgaccacttc gcgcaggagg tcggtggcga cctgggtata gagcgccgtc gtgtccaatt gcttgtgccc gagtagcacc tggatcacac gaatatccac cttctgctcc agcaggtgcg ttgcaaagca atgtcgaagc gtgtgcatcg agacgcgctt gtcgatcttc gcctccgcgg cggcggcatg gatagcccgg ttgagctggc gggtgctgag cgattcgatc gggttgaggc ccggaaacag ccagccgcca tcgagcatct tgccctgtgc gcgtgccaca cgccaccaca ctcgcaggcg ttcgagcagt actggtgaaa gcatggcgta gcgatccttg cggcctttgc cctgttcaac gcgcagcgtc atgcgttggc tgtcaatgtc gcaaaccttg agcgcgacaa cttcgctggc gcgcagccca gttccgtagg cgagcgacag tgccgtttga tgcttcaggt tgccagacgc cgcgatcagg cgacccactt cctcgcggct taatacgacg gggagtctct gcgggacacg aacgggttgc atcttggcca tcagctcggc acgatcgagc gtgacgtcga agaagaactt caatccggca atcgcggcat tgagcgagat cggcgaagtg ccggtatcga cgagatgcaa ctgataccac ctcagatctt ctacggtggc cgtatcaggc gagcggccga gaaaccccgc gaatcgtttc acggcgcgca ggtaaccgtt ttgtgtcttg gcagtgaatt tgcgcatccg catgtcgtcg atcatgcgct ggcgcagcgg actgatggtt tgagtggatg ggttcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc catccactca aaccatcagt ccgctgcgcc agcgcatgat cgacgacatg cggatgcgca aattcactgc caagacacaa aacggttacc tgcgcgccgt gaaacgattc gcggggtttc tcggccgctc gcctgatacg gccaccgtag aagatctgag gtggtatcag ttgcatctcg tcgataccgg cacttcgccg atctcgctca atgccgcgat tgccggattg aagttcttct tcgacgtcac gctcgatcgt gccgagctga tggccaagat gcaacccgtt cgtgtcccgc agagactccc cgtcgtatta agccgcgagg aagtgggtcg cctgatcgcg gcgtctggca acctgaagca tcaaacggca ctgtcgctcg cctacggaac tgggctgcgc gccagcgaag ttgtcgcgct caaggtttgc gacattgaca gccaacgcat gacgctgcgc gttgaacagg gcaaaggccg caaggatcgc tacgccatgc tttcaccagt actgctcgaa cgcctgcgag tgtggtggcg tgtggcacgc gcacagggca agatgctcga tggcggctgg ctgtttccgg gcctcaaccc gatcgaatcg ctcagcaccc gccagctcaa ccgggctatc catgccgccg ccgcggaggc gaagatcgac aagcgcgtct cgatgcacac gcttcgacat tgctttgcaa cgcacctgct ggagcagaag gtggatattc gtgtgatcca ggtgctactc gggcacaagc aattggacac gacggcgctc tatacccagg tcgccaccga cctcctgcgc gaagtggtca gccccctgga gatgctgcac ccggaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t