BuviA.00139.a
Dephospho-CoA kinase
| CENTER ID: | BuviA.00139.a |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00139.a.B1.GE35346 | Full length( BuviA.00139.a ) | 1 | 202 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00139.a.B1.PS01582 | Full length( BuviA.00139.a ) | 1 | 202 |
Structures
4I1U
DEPOSITED: 11/21/2012
DETERMINATION: XRay
CLONE: BuviA.00139.a.B1.GE35346
PROTEIN: BuviA.00139.a.B1.PS01581
4I1V
DEPOSITED: 11/21/2012
DETERMINATION: XRay
CLONE: BuviA.00139.a.B1.GE35346
PROTEIN: BuviA.00139.a.B1.PS01581
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127254 |
| PATRIC ID: | fig|269482.11.peg.4749 |
| RefSeq: | YP_001118397.1 |
| UniProt: | A4JBA9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYAIGLTGGI GSGKTTVADL FAARGASLVD TDLIAHRITA PAGLAMPAIE QTFGPAFVAA DGSLDRARMR ALIFSDEDAR RRLEAITHPL IRAETEREAR DAQGPYVIFV VPLLVESRNW KARCDRVLVV DCPVDTQIAR VMQRNGFTRE QVEAIIARQA TREARLAAAD DVIVNDAATP DALAVQVDAL HQRYLAFAAA KH
NT Sequence
ATGTATGCAA TAGGACTCAC CGGCGGGATC GGCAGCGGCA AGACGACCGT CGCCGACCTG TTCGCCGCAC GCGGCGCATC GCTGGTCGAT ACCGACCTGA TCGCGCACCG CATCACCGCG CCGGCCGGTC TCGCGATGCC GGCGATCGAG CAGACGTTCG GGCCCGCGTT CGTCGCGGCC GACGGCTCGC TCGACCGCGC ACGGATGCGC GCACTCATCT TCAGCGACGA AGACGCGCGC AGGCGGCTCG AAGCGATCAC GCATCCGCTG ATTCGCGCGG AGACGGAACG CGAGGCCCGC GACGCGCAGG GCCCGTACGT GATTTTCGTC GTGCCGCTGC TGGTCGAGTC GCGCAATTGG AAGGCGCGCT GCGATCGCGT GCTCGTCGTC GACTGCCCGG TCGACACCCA GATCGCGCGC GTGATGCAGC GCAATGGCTT CACGCGCGAG CAGGTCGAGG CGATCATCGC GCGACAAGCG ACGCGCGAAG CGCGCCTGGC CGCTGCCGAC GACGTGATCG TCAACGACGC GGCGACGCCC GACGCGCTGG CCGTGCAGGT CGATGCATTG CACCAGCGCT ACCTCGCATT CGCGGCTGCA AAACAC
Details for BuviA.00139.a.B1.GE35346
| HARVESTED ON: | 5/16/2012 |
| SEQUENCED ON: | 3/3/2015 |
| EXPECTED MW: | 22kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMY AIGLTGGIGS GKTTVADLFA ARGASLVDTD LIAHRITAPA GLAMPAIEQT FGPAFVAADG SLDRARMRAL IFSDEDARRR LEAITHPLIR AETEREARDA QGPYVIFVVP LLVESRNWKA RCDRVLVVDC PVDTQIARVM QRNGFTREQV EAIIARQATR EARLAAADDV IVNDAATPDA LAVQVDALHQ RYLAFAAAKH
Validated NT Sequence
ttaagaagga ganataccat ggctcaccac caccaccacc atatgtatgc aataggactc accggcggga tcggcagcgg caagacgacc gtcgccgacc tgttcgccgc acgcggcgca tcgctggtcg ataccgacct gatcgcgcac cgcatcaccg cgccggccgg tctcgcgatg ccggcgatcg agcagacgtt cgggcccgcg ttcgtcgcgg ccgacggctc gctcgaccgc gcacggatgc gcgcactcat cttcagcgac gaagacgcgc gcaggcggct cgaagcgatc acgcatccgc tgattcgcgc ggagacggaa cgcgaggccc gcgacgcgca gggcccgtac gtgattttcg tcgtgccgct gctggtcgag tcgcgcaatt ggaaggcgcg ctgcgatcgc gtgctcgtcg tcgactgccc ggtcgacacc cagatcgcgc gcgtgatgca gcgcaatggc ttcacgcgcg agcaggtcga ggcgatcatc gcgcgacaag cgacgcgcga agcgcgcctg gccgctgccg acgacgtgat cgtcaacgac gcggcgacgc ccgacgcgct ggccgtgcag gtcgatgcat tgcaccagcg ctacctcgca ttcgcggctg caaaacacta agtgagtaag at
Expected Protein Sequence
MAHHHHHHMY AIGLTGGIGS GKTTVADLFA ARGASLVDTD LIAHRITAPA GLAMPAIEQT FGPAFVAADG SLDRARMRAL IFSDEDARRR LEAITHPLIR AETEREARDA QGPYVIFVVP LLVESRNWKA RCDRVLVVDC PVDTQIARVM QRNGFTREQV EAIIARQATR EARLAAADDV IVNDAATPDA LAVQVDALHQ RYLAFAAAKH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatg caataggact caccggcggg atcggcagcg gcaagacgac cgtcgccgac ctgttcgccg cacgcggcgc atcgctggtc gataccgacc tgatcgcgca ccgcatcacc gcgccggccg gtctcgcgat gccggcgatc gagcagacgt tcgggcccgc gttcgtcgcg gccgacggct cgctcgaccg cgcacggatg cgcgcactca tcttcagcga cgaagacgcg cgcaggcggc tcgaagcgat cacgcatccg ctgattcgcg cggagacgga acgcgaggcc cgcgacgcgc agggcccgta cgtgattttc gtcgtgccgc tgctggtcga gtcgcgcaat tggaaggcgc gctgcgatcg cgtgctcgtc gtcgactgcc cggtcgacac ccagatcgcg cgcgtgatgc agcgcaatgg cttcacgcgc gagcaggtcg aggcgatcat cgcgcgacaa gcgacgcgcg aagcgcgcct ggccgctgcc gacgacgtga tcgtcaacga cgcggcgacg cccgacgcgc tggccgtgca ggtcgatgca ttgcaccagc gctacctcgc attcgcggct gcaaaacact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuviA.00139.a.B1.PS01582
| PURIFICATION DATe: | 7/24/2012 |
| CONCENTRATION: | 69.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMY AIGLTGGIGS GKTTVADLFA ARGASLVDTD LIAHRITAPA GLAMPAIEQT FGPAFVAADG SLDRARMRAL IFSDEDARRR LEAITHPLIR AETEREARDA QGPYVIFVVP LLVESRNWKA RCDRVLVVDC PVDTQIARVM QRNGFTREQV EAIIARQATR EARLAAADDV IVNDAATPDA LAVQVDALHQ RYLAFAAAKH
Validated NT Sequence
ttaagaagga ganataccat ggctcaccac caccaccacc atatgtatgc aataggactc accggcggga tcggcagcgg caagacgacc gtcgccgacc tgttcgccgc acgcggcgca tcgctggtcg ataccgacct gatcgcgcac cgcatcaccg cgccggccgg tctcgcgatg ccggcgatcg agcagacgtt cgggcccgcg ttcgtcgcgg ccgacggctc gctcgaccgc gcacggatgc gcgcactcat cttcagcgac gaagacgcgc gcaggcggct cgaagcgatc acgcatccgc tgattcgcgc ggagacggaa cgcgaggccc gcgacgcgca gggcccgtac gtgattttcg tcgtgccgct gctggtcgag tcgcgcaatt ggaaggcgcg ctgcgatcgc gtgctcgtcg tcgactgccc ggtcgacacc cagatcgcgc gcgtgatgca gcgcaatggc ttcacgcgcg agcaggtcga ggcgatcatc gcgcgacaag cgacgcgcga agcgcgcctg gccgctgccg acgacgtgat cgtcaacgac gcggcgacgc ccgacgcgct ggccgtgcag gtcgatgcat tgcaccagcg ctacctcgca ttcgcggctg caaaacacta agtgagtaag at
Expressed Protein Sequence
MAHHHHHHMY AIGLTGGIGS GKTTVADLFA ARGASLVDTD LIAHRITAPA GLAMPAIEQT FGPAFVAADG SLDRARMRAL IFSDEDARRR LEAITHPLIR AETEREARDA QGPYVIFVVP LLVESRNWKA RCDRVLVVDC PVDTQIARVM QRNGFTREQV EAIIARQATR EARLAAADDV IVNDAATPDA LAVQVDALHQ RYLAFAAAKH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTATG CAATAGGACT CACCGGCGGG ATCGGCAGCG GCAAGACGAC CGTCGCCGAC CTGTTCGCCG CACGCGGCGC ATCGCTGGTC GATACCGACC TGATCGCGCA CCGCATCACC GCGCCGGCCG GTCTCGCGAT GCCGGCGATC GAGCAGACGT TCGGGCCCGC GTTCGTCGCG GCCGACGGCT CGCTCGACCG CGCACGGATG CGCGCACTCA TCTTCAGCGA CGAAGACGCG CGCAGGCGGC TCGAAGCGAT CACGCATCCG CTGATTCGCG CGGAGACGGA ACGCGAGGCC CGCGACGCGC AGGGCCCGTA CGTGATTTTC GTCGTGCCGC TGCTGGTCGA GTCGCGCAAT TGGAAGGCGC GCTGCGATCG CGTGCTCGTC GTCGACTGCC CGGTCGACAC CCAGATCGCG CGCGTGATGC AGCGCAATGG CTTCACGCGC GAGCAGGTCG AGGCGATCAT CGCGCGACAA GCGACGCGCG AAGCGCGCCT GGCCGCTGCC GACGACGTGA TCGTCAACGA CGCGGCGACG CCCGACGCGC TGGCCGTGCA GGTCGATGCA TTGCACCAGC GCTACCTCGC ATTCGCGGCT GCAAAACACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT