SSGCID: Target Page

BuviA.00138.a

Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase

CENTER ID:	BuviA.00138.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00138.a.B1.GE39160	Full length( BuviA.00138.a )		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6604
RefSeq:	YP_001120197.1
UniProt:	A4JGF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTHALFRED AYLTRCEAVV AAVGDDGIRL DRTVFYPLGG GQAGDAGTLT LPDGGTIAIA DTHKARFDGA TPDDAVHVPA PGQEARIAAL RPGARVVADI DWARRYRHMR LHTAAHLMCA VLPYPVDGCS VTADYVRLDF ATSDAIERAD VEQRLAALVH GAHAVTTEWI TDAEMAERPE LVRTMSVKPP MGLGRVRLLR IEGVDLQPCG GTHVRNTAEV GALRVAKLEK KSARTRRVVL AFA

NT Sequence

ATGACGACCC ACGCGCTGTT CCGTGAAGAC GCCTACCTGA CACGATGCGA GGCCGTCGTC GCGGCCGTCG GCGACGACGG CATCCGGCTC GACCGCACCG TGTTCTATCC GCTCGGCGGC GGCCAGGCCG GCGACGCCGG GACGCTCACG CTGCCCGACG GCGGCACGAT CGCGATCGCC GACACGCACA AGGCGCGCTT CGACGGCGCC ACGCCCGACG ACGCCGTGCA CGTGCCGGCG CCCGGCCAGG AGGCGCGCAT CGCCGCGCTG CGGCCCGGCG CGCGCGTGGT GGCCGACATC GACTGGGCGC GCCGCTACCG GCACATGCGG CTGCACACGG CCGCGCACCT GATGTGCGCG GTGCTGCCGT ATCCGGTCGA CGGCTGCAGC GTGACGGCCG ACTACGTGCG GCTCGATTTC GCGACGTCGG ACGCGATCGA GCGCGCCGAC GTCGAACAGC GGCTCGCGGC GCTGGTGCAC GGCGCGCATG CGGTCACGAC CGAATGGATC ACCGACGCCG AAATGGCCGA GCGCCCCGAA CTCGTCCGCA CGATGAGCGT GAAGCCGCCG ATGGGGCTCG GCCGCGTGCG CTTGCTGCGC ATCGAAGGCG TCGATCTGCA GCCGTGCGGC GGCACGCACG TGCGCAACAC GGCGGAAGTT GGCGCGCTGC GGGTCGCGAA GCTCGAGAAG AAGAGTGCGC GGACACGGCG CGTGGTGCTG GCGTTCGCA

Details for BuviA.00138.a.B1.GE39160

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	27kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	23
PERCENT COVERAGE:	66

Validated AA Sequence

MRAVHQRREP LFDVGALDRV RRREIEPHVV GRHAAAVDRI RQHRAHQVRG RVQPHVPVAA RPVDVGHHAR AGPQRGDARL LAGRRHVHGV VGRGAVEARL VRVGDRDRAA VGQRERPGVA GLAAAERIEH GAVEPDAVXX RXPRRRPRXV SXXXXXXXXX XXXYXX

Validated NT Sequence

gcgcactctt cttctcgagc ttcgcgaccc gcagcgcgcc aacttccgcc gtgttgcgca cgtgcgtgcc gccgcacggc tgcagatcga cgccttcgat gcgcagcaag cgcacgcggc cgagccccat cggcggcttc acgctcatcg tgcggacgag ttcggggcgc tcggccattt cggcgtcggt gatccattcg gtcgtgaccg catgcgcgcc gtgcaccagc gccgcgagcc gctgttcgac gtcggcgcgc tcgatcgcgt ccgacgtcgc gaaatcgagc cgcacgtagt cggccgtcac gctgcagccg tcgaccggat acggcagcac cgcgcacatc aggtgcgcgg ccgtgtgcag ccgcatgtgc cggtagcggc gcgcccagtc gatgtcggcc accacgcgcg cgccgggccg cagcgcggcg atgcgcgcct cctggccggg cgccggcacg tgcacggcgt cgtcgggcgt ggcgccgtcg aagcgcgcct tgtgcgtgtc ggcgatcgcg atcgtgccgc cgtcgggcag cgtgagcgtc ccggcgtcgc cggcctggcc gccgccgagc ggatagaaca cggtgcggtc gagccggatg ccgtnannnn ncgacnnccg cgacgacggc ctcgcancgt gtcannnnnn nnnnnnnnnn nnnnannnnn nnnnnnnnnn tatgnnnntg

Expected Protein Sequence

MAHHHHHHMT THALFREDAY LTRCEAVVAA VGDDGIRLDR TVFYPLGGGQ AGDAGTLTLP DGGTIAIADT HKARFDGATP DDAVHVPAPG QEARIAALRP GARVVADIDW ARRYRHMRLH TAAHLMCAVL PYPVDGCSVT ADYVRLDFAT SDAIERADVE QRLAALVHGA HAVTTEWITD AEMAERPELV RTMSVKPPMG LGRVRLLRIE GVDLQPCGGT HVRNTAEVGA LRVAKLEKKS ARTRRVVLAF A

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cccacgcgct gttccgtgaa gacgcctacc tgacacgatg cgaggccgtc gtcgcggccg tcggcgacga cggcatccgg ctcgaccgca ccgtgttcta tccgctcggc ggcggccagg ccggcgacgc cgggacgctc acgctgcccg acggcggcac gatcgcgatc gccgacacgc acaaggcgcg cttcgacggc gccacgcccg acgacgccgt gcacgtgccg gcgcccggcc aggaggcgcg catcgccgcg ctgcggcccg gcgcgcgcgt ggtggccgac atcgactggg cgcgccgcta ccggcacatg cggctgcaca cggccgcgca cctgatgtgc gcggtgctgc cgtatccggt cgacggctgc agcgtgacgg ccgactacgt gcggctcgat ttcgcgacgt cggacgcgat cgagcgcgcc gacgtcgaac agcggctcgc ggcgctggtg cacggcgcgc atgcggtcac gaccgaatgg atcaccgacg ccgaaatggc cgagcgcccc gaactcgtcc gcacgatgag cgtgaagccg ccgatggggc tcggccgcgt gcgcttgctg cgcatcgaag gcgtcgatct gcagccgtgc ggcggcacgc acgtgcgcaa cacggcggaa gttggcgcgc tgcgggtcgc gaagctcgag aagaagagtg cgcggacacg gcgcgtggtg ctggcgttcg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat