BuviA.00137.a

UDP-N-acetylmuramate

CENTER ID: BuviA.00137.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00137.a.B2.GE35356 Mature protein( BuviA.00137.a ) 20 461
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4785
RefSeq: YP_001118431.1
UniProt: A4JBE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIHILGICG TFMGGLAVLA REAGHTVTGC DAGVYPPMST QLEAQGITLI EGYGAEQIDL KPDLFVIGNV VTRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWLLEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEYDH ADIFPDLAAI ETQFHHLVRT VPRVGRIVTN GRSDALERVL ARGCWSEVER FGVDGGWQAL PAEDGVPVDE RFAVYSQAQR VGEVAWQVQG DHNRMNALAA IAAARHVGVP PAQAAASLAS FRNVKRRMEV RGSVDGVTVY DDFAHHPTAI ETTIAGLRAR IGREHSRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPSGRDAL GWNLGDALAP LGAKAHAFDD LHLLVKAVVA AARPGDHVLV MSNGGFGGVH QQLLDALGSR A
NT Sequence
ATGCACATCC ACATTCTTGG CATCTGCGGC ACCTTCATGG GCGGGCTCGC CGTGCTCGCG CGCGAGGCGG GCCACACGGT GACGGGTTGC GACGCGGGCG TCTATCCGCC GATGAGCACG CAGCTCGAGG CGCAGGGCAT CACGCTGATC GAAGGCTACG GCGCCGAGCA GATCGACCTG AAACCCGACC TCTTCGTGAT CGGCAACGTC GTCACGCGCG GCAATCCGCT GATGGAGGCG ATCCTCGATC GCGGCCTGCC TTACGTGTCC GGCCCGCAAT GGCTCGGCGA ACACGTGCTG GCCGGCAAAT GGGTGCTCGC GGTCGCGGGC ACGCACGGCA AGACGACCAC CTCGTCGATG CTCGCGTGGC TGCTGGAAGA CGCCGGCCTG AATCCGGGCT TCCTGATCGG CGGCGTGCCG CTGAACTTCG GCGTGTCGGC GCGGCTGACC GATTCGAGCT TCTTCGTGAT CGAAGCCGAC GAATACGATA CGGCGTTCTT CGACAAGCGC TCGAAGTTCG TCCACTACCG GCCGCGCACG GCCGTGCTGA ACAATCTCGA ATACGATCAC GCCGACATCT TCCCGGATCT CGCCGCGATC GAAACGCAAT TCCACCATCT CGTGCGCACG GTGCCGCGGG TGGGCCGGAT CGTCACGAAC GGCCGTTCCG ACGCGCTGGA GCGCGTGCTG GCGCGCGGCT GCTGGAGCGA AGTCGAGCGT TTCGGCGTCG ACGGCGGCTG GCAGGCGCTG CCGGCCGAGG ACGGTGTGCC GGTGGACGAG CGCTTCGCGG TGTATTCACA GGCCCAGCGC GTCGGCGAAG TCGCGTGGCA GGTGCAGGGC GACCACAACC GGATGAACGC GCTGGCCGCG ATCGCGGCCG CGCGCCATGT CGGCGTACCG CCGGCGCAGG CGGCCGCCTC GCTCGCATCG TTCCGTAACG TGAAGCGGCG CATGGAAGTG CGCGGCAGCG TCGACGGCGT GACCGTCTAC GACGACTTCG CGCACCATCC GACCGCGATC GAAACCACGA TCGCCGGCCT TCGTGCGCGC ATCGGCCGAG AACATTCCCG CATCCTCGCC GTGCTGGAGC CGCGTTCGAA CACGATGAAG CTCGGCGTGA TGAAGGCGCA GCTGCCGGCC AGCCTCGCCG ATGCCGACCT CGTGTTCGGC TACGGCGCGC CGAGCGGGCG CGACGCGCTC GGCTGGAACC TCGGCGACGC ACTGGCGCCG CTCGGTGCGA AGGCGCACGC GTTCGACGAC CTGCATCTGT TGGTGAAGGC CGTCGTCGCC GCCGCCCGCC CGGGCGACCA CGTGCTGGTG ATGAGCAACG GCGGCTTCGG CGGCGTGCAC CAGCAGCTGC TCGACGCGCT CGGGAGCCGC GCG
Details for BuviA.00137.a.B2.GE35356
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMA REAGHTVTGC DAGVYPPMST QLEAQGITLI EGYGAEQIDL KPDLFVIGNV VTRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWLLEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEYDH ADIFPDLAAI ETQFHHLVRT VPRVGRIVTN GRSDALERVL ARGCWSEVER FGVDGGWQAL PAEDGVPVDE RFAVYSQAQR VGEVAWQVQG DHNRMNALAA IAAARHVGVP PAQAAASLAS FRNVKRRMXX ARXRXRRDRL RRLXAXSDXD RNXXXXXSXX HXXXXXXIXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttgtttaa ctttaagaag gaganatacc atggctcacc accaccacca ccatatggcg cgcgaggcgg gccacacggt gacgggttgc gacgcgggcg tctatccgcc gatgagcacg cagctcgagg cgcagggcat cacgctgatc gaaggctacg gcgccgagca gatcgacctg aaacccgacc tcttcgtgat cggcaacgtc gtcacgcgcg gcaatccgct gatggaggcg atcctcgatc gcggcctgcc ttacgtgtcc ggcccgcaat ggctcggcga acacgtgctg gccggcaaat gggtgctcgc ggtcgcgggc acgcacggca agacgaccac ctcgtcgatg ctcgcgtggc tgctggaaga cgccggcctg aatccgggct tcctgatcgg cggcgtgccg ctgaacttcg gcgtgtcggc gcggctgacc gattcgagct tcttcgtgat cgaagccgac gaatacgata cggcgttctt cgacaagcgc tcgaagttcg tccactaccg gccgcgcacg gccgtgctga acaatctcga atacgatcac gccgacatct tcccggatct cgccgcgatc gaaacgcaat tccaccatct cgtgcgcacg gtgccgcggg tgggccggat cgtcacgaac ggccgttccg acgcgctgga gcgcgtgctg gcgcgcggct gctggagcga agtcgagcgt ttcggcgtcg acggcggctg gcaggcgctg ccggccgagg acggtgtgcc ggtggacgag cgcttcgcgg tgtattcaca ggcccagcgc gtcggcgaag tcgcgtggca ggtgcagggc gaccacaacc ggatgaacgc gctggccgcg atcgcggccg cgcgccatgt cggcgtaccg ccggcgcagg cggccgcctc gctcgcatcg ttccgtaacg tgaagcggcg catggnannt gcgcggcanc gtcnacggcg tgaccgtcta cgacgacttc nngcancatc cgaccnngat cgaaaccncn atcnnnngnn ntcnngcnnn catcngncga nanattncng catcntcnng nnngnncnnc ntnnnannnn annnnnnnng nnnnnnnnnn nnncnncnnn nnnnnncntc nnnnannnnn nnnnnnnnnn nnnannnnng g
Expected Protein Sequence
MAHHHHHHMA REAGHTVTGC DAGVYPPMST QLEAQGITLI EGYGAEQIDL KPDLFVIGNV VTRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWLLEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEYDH ADIFPDLAAI ETQFHHLVRT VPRVGRIVTN GRSDALERVL ARGCWSEVER FGVDGGWQAL PAEDGVPVDE RFAVYSQAQR VGEVAWQVQG DHNRMNALAA IAAARHVGVP PAQAAASLAS FRNVKRRMEV RGSVDGVTVY DDFAHHPTAI ETTIAGLRAR IGREHSRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPSGRDAL GWNLGDALAP LGAKAHAFDD LHLLVKAVVA AARPGDHVLV MSNGGFGGVH QQLLDALGSR A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc gcgaggcggg ccacacggtg acgggttgcg acgcgggcgt ctatccgccg atgagcacgc agctcgaggc gcagggcatc acgctgatcg aaggctacgg cgccgagcag atcgacctga aacccgacct cttcgtgatc ggcaacgtcg tcacgcgcgg caatccgctg atggaggcga tcctcgatcg cggcctgcct tacgtgtccg gcccgcaatg gctcggcgaa cacgtgctgg ccggcaaatg ggtgctcgcg gtcgcgggca cgcacggcaa gacgaccacc tcgtcgatgc tcgcgtggct gctggaagac gccggcctga atccgggctt cctgatcggc ggcgtgccgc tgaacttcgg cgtgtcggcg cggctgaccg attcgagctt cttcgtgatc gaagccgacg aatacgatac ggcgttcttc gacaagcgct cgaagttcgt ccactaccgg ccgcgcacgg ccgtgctgaa caatctcgaa tacgatcacg ccgacatctt cccggatctc gccgcgatcg aaacgcaatt ccaccatctc gtgcgcacgg tgccgcgggt gggccggatc gtcacgaacg gccgttccga cgcgctggag cgcgtgctgg cgcgcggctg ctggagcgaa gtcgagcgtt tcggcgtcga cggcggctgg caggcgctgc cggccgagga cggtgtgccg gtggacgagc gcttcgcggt gtattcacag gcccagcgcg tcggcgaagt cgcgtggcag gtgcagggcg accacaaccg gatgaacgcg ctggccgcga tcgcggccgc gcgccatgtc ggcgtaccgc cggcgcaggc ggccgcctcg ctcgcatcgt tccgtaacgt gaagcggcgc atggaagtgc gcggcagcgt cgacggcgtg accgtctacg acgacttcgc gcaccatccg accgcgatcg aaaccacgat cgccggcctt cgtgcgcgca tcggccgaga acattcccgc atcctcgccg tgctggagcc gcgttcgaac acgatgaagc tcggcgtgat gaaggcgcag ctgccggcca gcctcgccga tgccgacctc gtgttcggct acggcgcgcc gagcgggcgc gacgcgctcg gctggaacct cggcgacgca ctggcgccgc tcggtgcgaa ggcgcacgcg ttcgacgacc tgcatctgtt ggtgaaggcc gtcgtcgccg ccgcccgccc gggcgaccac gtgctggtga tgagcaacgg cggcttcggc ggcgtgcacc agcagctgct cgacgcgctc gggagccgcg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat