BuviA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: BuviA.00134.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00134.a.B1.GE39159 Full length( BuviA.00134.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00134.a.B1.PW37798 Full length( BuviA.00134.a ) 1 375

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3652
RefSeq: YP_001117343.1
UniProt: A4JNM5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPGNSHPLSS DTPPVADPAA NPLGMAGLEF VEFAAPVPDA LAQRFEQLGF KAVARHVSKN VTLYRQGQMQ FLINAEPDSF AARYAEEYGM GVCAIGLRVA SARRAFERAI ELGAWAFEGE RVGVGELKIP AIQGIGDSHL YFVDRWRGRD GQRGGVGDIS IFDIDFRPID IATAHADLHC EGTGLQQVDH FTQTVGAGRM REWLDFYHDL LHFREIHEID AHWHVSEESR VMVSPCGAVR IPVYEEGTRR TELMHAYLPD HPGEGVQHIA LATDDIVASV DALRANGVDF VEPPARYYDE VDARLPGHGV DLDALRRCAV LVDGEIGADG VPRLFFQTFV KRRPGEIFFE IVQRQGHHGF GEGNLAALAR ARDAG
NT Sequence
ATGCCCGGCA ATTCTCACCC ACTGTCCAGC GATACGCCGC CCGTTGCCGA TCCGGCCGCC AATCCGCTCG GGATGGCCGG ACTCGAATTC GTCGAGTTCG CGGCGCCGGT GCCGGACGCG CTCGCGCAGC GCTTCGAGCA ACTCGGGTTC AAGGCTGTCG CGCGCCATGT CAGCAAGAAC GTCACACTGT ACCGGCAAGG ACAGATGCAG TTCCTGATCA ATGCGGAGCC CGATTCATTC GCCGCTCGCT ATGCGGAGGA GTACGGGATG GGCGTGTGCG CGATCGGGCT GCGCGTGGCA AGCGCCCGGC GTGCGTTCGA GCGGGCGATC GAGCTGGGGG CGTGGGCGTT CGAGGGCGAG CGGGTCGGCG TCGGCGAACT GAAGATTCCG GCGATCCAGG GCATCGGCGA TTCGCACCTG TACTTCGTCG ATCGCTGGCG CGGGCGCGAC GGTCAGCGCG GCGGCGTGGG CGACATCTCG ATCTTCGACA TCGATTTCCG GCCGATCGAC ATCGCGACCG CGCACGCCGA TCTCCACTGC GAGGGCACCG GGCTGCAACA GGTCGACCAC TTCACGCAGA CGGTCGGCGC GGGGCGCATG CGCGAGTGGC TCGACTTCTA CCACGACCTG CTGCACTTTC GCGAAATCCA CGAGATCGAC GCGCACTGGC ACGTGTCGGA GGAATCGCGC GTGATGGTGT CGCCCTGCGG CGCGGTGCGG ATTCCGGTCT ACGAGGAGGG CACGCGCCGC ACCGAGCTGA TGCACGCGTA TCTGCCCGAT CATCCCGGTG AGGGCGTGCA GCACATCGCG CTCGCGACCG ACGATATCGT CGCGAGCGTC GATGCGCTGC GCGCGAACGG CGTCGACTTC GTCGAGCCGC CTGCGCGCTA TTACGACGAA GTCGATGCGC GATTGCCGGG GCACGGCGTC GATCTCGACG CGTTGCGCCG CTGCGCGGTG CTGGTCGACG GCGAAATCGG CGCCGACGGC GTGCCGCGCC TGTTCTTCCA GACCTTCGTC AAACGCCGGC CCGGCGAGAT CTTCTTCGAG ATCGTGCAGC GTCAGGGGCA TCACGGGTTC GGCGAAGGAA ACCTCGCCGC GCTGGCGCGC GCCCGCGACG CCGGC
Details for BuviA.00134.a.B1.GE39159
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MPGNSHPLSS DTPPVADPAA NPLGMAGLEF VEFAAPVPDA LAQRFEQLGF KAVARHVSKN VTLYRQGQMQ FLINAEPDSF AARYAEEYGM GVCAIGLRVA SARRAFERAI ELGAWAFEGE RVGVGELKIP AIQGIGDSHL YFVDRWRGRD GQRGGVGDIS IFDIDFRPID IATAHADLHC EGTGLQQVDH FTQTVGAGRM REWLDFYHDL LHFREIHEID AHWHVSEESR VMVSPCGAVR IPVYEEGTRR TELMHAYLPD HPGEGVQHIA LATDDIVASV DALRANGVDF VEPPARYYDE VDARLPGHGV DLDALRRCAV LVDGEIGADG VPRLFFQTFV KRRPGEIFFE IVQRQGHHGF GEGNLA
Validated NT Sequence
gcggcgaggt ttccttcgcc gaacccgtga tgcccctgac gctgcacgat ctcgaagaag atctcgccgg gccggcgttt gacgaaggtc tggaagaaca ggcgcggcac gccgtcggcg ccgatttcgc cgtcgaccag caccgcgcag cggcgcaacg cgtcgagatc gacgccgtgc cccggcaatc gcgcatcgac ttcgtcgtaa tagcgcgcag gcggctcgac gaagtcgacg ccgttcgcgc gcagcgcatc gacgctcgcg acgatatcgt cggtcgcgag cgcgatgtgc tgcacgccct caccgggatg atcgggcaga tacgcgtgca tcagctcggt gcggcgcgtg ccctcctcgt agaccggaat ccgcaccgcg ccgcagggcg acaccatcac gcgcgattcc tccgacacgt gccagtgcgc gtcgatctcg tggatttcgc gaaagtgcag caggtcgtgg tagaagtcga gccactcgcg catgcgcccc gcgccgaccg tctgcgtgaa gtggtcgacc tgttgcagcc cggtgccctc gcagtggaga tcggcgtgcg cggtcgcgat gtcgatcggc cggaaatcga tgtcgaagat cgagatgtcg cccacgccgc cgcgctgacc gtcgcgcccg cgccagcgat cgacgaagta caggtgcgaa tcgccgatgc cctggatcgc cggaatcttc agttcgccga cgccgacccg ctcgccctcg aacgcccacg cccccagctc gatcgcccgc tcgaacgcac gccgggcgct tgccacgcgc agcccgatcg cgcacacgcc catcccgtac tcctccgcat agcgagcggc gaatgaatcg ggctccgcat tgatcaggaa ctgcatctgt ccttgccggt acagtgtgac gttcttgctg acatggcgcg cgacagcctt gaacccgagt tgctcgaagc gctgcgcgag cgcgtccggc accggcgccg cgaactcgac gaattcgagt ccggccatcc cgagcggatt ggcggccgga tcggcaacgg gcggcgtatc gctggacagt gggtgagaat tgccgggcat atggtggtgg tggtgtgnnn nnnat
Expected Protein Sequence
MAHHHHHHMP GNSHPLSSDT PPVADPAANP LGMAGLEFVE FAAPVPDALA QRFEQLGFKA VARHVSKNVT LYRQGQMQFL INAEPDSFAA RYAEEYGMGV CAIGLRVASA RRAFERAIEL GAWAFEGERV GVGELKIPAI QGIGDSHLYF VDRWRGRDGQ RGGVGDISIF DIDFRPIDIA TAHADLHCEG TGLQQVDHFT QTVGAGRMRE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGAVRIP VYEEGTRRTE LMHAYLPDHP GEGVQHIALA TDDIVASVDA LRANGVDFVE PPARYYDEVD ARLPGHGVDL DALRRCAVLV DGEIGADGVP RLFFQTFVKR RPGEIFFEIV QRQGHHGFGE GNLAALARAR DAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg gcaattctca cccactgtcc agcgatacgc cgcccgttgc cgatccggcc gccaatccgc tcgggatggc cggactcgaa ttcgtcgagt tcgcggcgcc ggtgccggac gcgctcgcgc agcgcttcga gcaactcggg ttcaaggctg tcgcgcgcca tgtcagcaag aacgtcacac tgtaccggca aggacagatg cagttcctga tcaatgcgga gcccgattca ttcgccgctc gctatgcgga ggagtacggg atgggcgtgt gcgcgatcgg gctgcgcgtg gcaagcgccc ggcgtgcgtt cgagcgggcg atcgagctgg gggcgtgggc gttcgagggc gagcgggtcg gcgtcggcga actgaagatt ccggcgatcc agggcatcgg cgattcgcac ctgtacttcg tcgatcgctg gcgcgggcgc gacggtcagc gcggcggcgt gggcgacatc tcgatcttcg acatcgattt ccggccgatc gacatcgcga ccgcgcacgc cgatctccac tgcgagggca ccgggctgca acaggtcgac cacttcacgc agacggtcgg cgcggggcgc atgcgcgagt ggctcgactt ctaccacgac ctgctgcact ttcgcgaaat ccacgagatc gacgcgcact ggcacgtgtc ggaggaatcg cgcgtgatgg tgtcgccctg cggcgcggtg cggattccgg tctacgagga gggcacgcgc cgcaccgagc tgatgcacgc gtatctgccc gatcatcccg gtgagggcgt gcagcacatc gcgctcgcga ccgacgatat cgtcgcgagc gtcgatgcgc tgcgcgcgaa cggcgtcgac ttcgtcgagc cgcctgcgcg ctattacgac gaagtcgatg cgcgattgcc ggggcacggc gtcgatctcg acgcgttgcg ccgctgcgcg gtgctggtcg acggcgaaat cggcgccgac ggcgtgccgc gcctgttctt ccagaccttc gtcaaacgcc ggcccggcga gatcttcttc gagatcgtgc agcgtcaggg gcatcacggg ttcggcgaag gaaacctcgc cgcgctggcg cgcgcccgcg acgccggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuviA.00134.a.B1.PW37798
PURIFICATION DATe: 10/13/2015
CONCENTRATION: 31mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MPGNSHPLSS DTPPVADPAA NPLGMAGLEF VEFAAPVPDA LAQRFEQLGF KAVARHVSKN VTLYRQGQMQ FLINAEPDSF AARYAEEYGM GVCAIGLRVA SARRAFERAI ELGAWAFEGE RVGVGELKIP AIQGIGDSHL YFVDRWRGRD GQRGGVGDIS IFDIDFRPID IATAHADLHC EGTGLQQVDH FTQTVGAGRM REWLDFYHDL LHFREIHEID AHWHVSEESR VMVSPCGAVR IPVYEEGTRR TELMHAYLPD HPGEGVQHIA LATDDIVASV DALRANGVDF VEPPARYYDE VDARLPGHGV DLDALRRCAV LVDGEIGADG VPRLFFQTFV KRRPGEIFFE IVQRQGHHGF GEGNLA
Validated NT Sequence
gcggcgaggt ttccttcgcc gaacccgtga tgcccctgac gctgcacgat ctcgaagaag atctcgccgg gccggcgttt gacgaaggtc tggaagaaca ggcgcggcac gccgtcggcg ccgatttcgc cgtcgaccag caccgcgcag cggcgcaacg cgtcgagatc gacgccgtgc cccggcaatc gcgcatcgac ttcgtcgtaa tagcgcgcag gcggctcgac gaagtcgacg ccgttcgcgc gcagcgcatc gacgctcgcg acgatatcgt cggtcgcgag cgcgatgtgc tgcacgccct caccgggatg atcgggcaga tacgcgtgca tcagctcggt gcggcgcgtg ccctcctcgt agaccggaat ccgcaccgcg ccgcagggcg acaccatcac gcgcgattcc tccgacacgt gccagtgcgc gtcgatctcg tggatttcgc gaaagtgcag caggtcgtgg tagaagtcga gccactcgcg catgcgcccc gcgccgaccg tctgcgtgaa gtggtcgacc tgttgcagcc cggtgccctc gcagtggaga tcggcgtgcg cggtcgcgat gtcgatcggc cggaaatcga tgtcgaagat cgagatgtcg cccacgccgc cgcgctgacc gtcgcgcccg cgccagcgat cgacgaagta caggtgcgaa tcgccgatgc cctggatcgc cggaatcttc agttcgccga cgccgacccg ctcgccctcg aacgcccacg cccccagctc gatcgcccgc tcgaacgcac gccgggcgct tgccacgcgc agcccgatcg cgcacacgcc catcccgtac tcctccgcat agcgagcggc gaatgaatcg ggctccgcat tgatcaggaa ctgcatctgt ccttgccggt acagtgtgac gttcttgctg acatggcgcg cgacagcctt gaacccgagt tgctcgaagc gctgcgcgag cgcgtccggc accggcgccg cgaactcgac gaattcgagt ccggccatcc cgagcggatt ggcggccgga tcggcaacgg gcggcgtatc gctggacagt gggtgagaat tgccgggcat atggtggtgg tggtgtgnnn nnnat
Expressed Protein Sequence
MAHHHHHHMP GNSHPLSSDT PPVADPAANP LGMAGLEFVE FAAPVPDALA QRFEQLGFKA VARHVSKNVT LYRQGQMQFL INAEPDSFAA RYAEEYGMGV CAIGLRVASA RRAFERAIEL GAWAFEGERV GVGELKIPAI QGIGDSHLYF VDRWRGRDGQ RGGVGDISIF DIDFRPIDIA TAHADLHCEG TGLQQVDHFT QTVGAGRMRE WLDFYHDLLH FREIHEIDAH WHVSEESRVM VSPCGAVRIP VYEEGTRRTE LMHAYLPDHP GEGVQHIALA TDDIVASVDA LRANGVDFVE PPARYYDEVD ARLPGHGVDL DALRRCAVLV DGEIGADGVP RLFFQTFVKR RPGEIFFEIV QRQGHHGFGE GNLAALARAR DAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCG GCAATTCTCA CCCACTGTCC AGCGATACGC CGCCCGTTGC CGATCCGGCC GCCAATCCGC TCGGGATGGC CGGACTCGAA TTCGTCGAGT TCGCGGCGCC GGTGCCGGAC GCGCTCGCGC AGCGCTTCGA GCAACTCGGG TTCAAGGCTG TCGCGCGCCA TGTCAGCAAG AACGTCACAC TGTACCGGCA AGGACAGATG CAGTTCCTGA TCAATGCGGA GCCCGATTCA TTCGCCGCTC GCTATGCGGA GGAGTACGGG ATGGGCGTGT GCGCGATCGG GCTGCGCGTG GCAAGCGCCC GGCGTGCGTT CGAGCGGGCG ATCGAGCTGG GGGCGTGGGC GTTCGAGGGC GAGCGGGTCG GCGTCGGCGA ACTGAAGATT CCGGCGATCC AGGGCATCGG CGATTCGCAC CTGTACTTCG TCGATCGCTG GCGCGGGCGC GACGGTCAGC GCGGCGGCGT GGGCGACATC TCGATCTTCG ACATCGATTT CCGGCCGATC GACATCGCGA CCGCGCACGC CGATCTCCAC TGCGAGGGCA CCGGGCTGCA ACAGGTCGAC CACTTCACGC AGACGGTCGG CGCGGGGCGC ATGCGCGAGT GGCTCGACTT CTACCACGAC CTGCTGCACT TTCGCGAAAT CCACGAGATC GACGCGCACT GGCACGTGTC GGAGGAATCG CGCGTGATGG TGTCGCCCTG CGGCGCGGTG CGGATTCCGG TCTACGAGGA GGGCACGCGC CGCACCGAGC TGATGCACGC GTATCTGCCC GATCATCCCG GTGAGGGCGT GCAGCACATC GCGCTCGCGA CCGACGATAT CGTCGCGAGC GTCGATGCGC TGCGCGCGAA CGGCGTCGAC TTCGTCGAGC CGCCTGCGCG CTATTACGAC GAAGTCGATG CGCGATTGCC GGGGCACGGC GTCGATCTCG ACGCGTTGCG CCGCTGCGCG GTGCTGGTCG ACGGCGAAAT CGGCGCCGAC GGCGTGCCGC GCCTGTTCTT CCAGACCTTC GTCAAACGCC GGCCCGGCGA GATCTTCTTC GAGATCGTGC AGCGTCAGGG GCATCACGGG TTCGGCGAAG GAAACCTCGC CGCGCTGGCG CGCGCCCGCG ACGCCGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT