BuviA.00120.a

GTPase obg

CENTER ID: BuviA.00120.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00120.a.A1.GE32434 Full length( BuviA.00120.a ) 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00120.a.A1.PS01327 Full length( BuviA.00120.a ) 1 370

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4758
RefSeq: YP_001118406.1
UniProt: A4JBB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGNVYAIAD RNINTLIDYR YAKKHMARNG ENGRGSDCYG KGGDDITLRM PVGTVVTDMD TGELIADLTE HDQQVMLAKG GSGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DESVDPVAEA KAIVGELRKY DEALYEKPRW LVLNKLDMVP EDERDARVAD FLERFGWDGP VFQISALTGQ GCEALCYAIY DYLAEHSDAH RAAEEEDLAA DVRFRDAPPS DGGATSGGDA
NT Sequence
ATGAAGTTCA TTGACGAAGC ACGAATCGAA GTCATCGCCG GCGACGGAGG CGATGGCAGC GCGTCGATGC GCCGCGAGAA GTTCGTTCCG TTCGGCGGGC CGGACGGCGG CGACGGCGGC CGGGGCGGTA ACGTCTACGC GATCGCCGAC CGCAACATCA ACACGCTGAT CGACTACCGT TACGCGAAGA AGCACATGGC GCGCAACGGC GAAAACGGTC GCGGCTCCGA TTGTTACGGC AAGGGCGGCG ACGACATCAC GCTGCGCATG CCGGTCGGCA CGGTCGTGAC CGACATGGAC ACCGGCGAGC TGATTGCCGA CCTGACCGAA CACGATCAGC AGGTGATGCT CGCGAAGGGC GGCTCCGGCG GCCTCGGCAA CCTGCATTTC AAGTCGAGCA CCAACCGGGC GCCGCGTCAG AAGACCGACG GCAAGCCCGG CGAGCGGCGC ATGCTGCGGC TCGAACTGAA GGTGCTCGCC GACGTCGGGC TGCTCGGCAT GCCGAACGCC GGCAAGTCGA CCTTCATTTC ATCGGTGTCG AACGCGAAGC CGAAGATCGC CGACTACCCG TTCACCACGC TCGCGCCGAA CCTGGGCGTC GTGCGGGTCG GCCCGAGCAA GAGCTTCGTG ATTGCCGATA TCCCTGGGCT GATCGAAGGC GCGGCCGAAG GCGCGGGCCT CGGCCACCAG TTCCTGCGGC ACCTGCAGCG CACCGGCGTG CTGCTGCATC TCGTCGACCT CGCTCCGTTC GACGAAAGCG TCGATCCGGT CGCGGAAGCG AAGGCGATCG TCGGCGAACT GCGCAAGTAT GACGAGGCGC TCTACGAAAA GCCGCGCTGG CTCGTGCTGA ACAAGCTCGA CATGGTGCCG GAAGACGAGC GCGACGCGCG CGTTGCCGAC TTCCTCGAGC GTTTCGGCTG GGACGGCCCG GTGTTCCAGA TTTCGGCCCT GACCGGCCAG GGCTGCGAAG CCCTGTGCTA TGCGATCTAC GATTACCTTG CCGAGCATTC GGACGCGCAT CGCGCGGCGG AAGAGGAAGA TCTGGCCGCG GACGTGCGCT TCCGCGATGC GCCGCCGAGC GACGGCGGCG CAACCTCAGG CGGCGATGCC
Details for BuviA.00120.a.A1.GE32434
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGNVYAIAD RNINTLIDYR YAKKHMARNG ENGRGSDCYG KGGDDITLRM PVGTVVTDMD TGELIADLTE HDQQVMLAKG GSGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DESVDPVAEA KAIVGELRKY DEALYEKPRW LVLNKLDMVP EDERDARVAD FLERFGWDGP VFQISALTGQ GCEALCYAIY DYLAEHSDAH RAAEEEDLAA DVRFRDAPPS DGGATSGG
Validated NT Sequence
cgccgcctga ggttgcgccg ccgtcgctcg gcggcgcatc gcggaagcgc acgtccgcgg ccagatcttc ctcttccgcc gcgcgatgcg cgtccgaatg ctcggcaagg taatcgtaga tcgcatagca cagggcttcg cagccctggc cggtcagggc cgaaatctgg aacaccgggc cgtcccagcc gaaacgctcg aggaagtcgg caacgcgcgc gtcgcgctcg tcttccggca ccatgtcgag cttgttcagc acgagccagc gcggcttttc gtagagcgcc tcgtcatact tgcgcagttc gccgacgatc gccttcgctt ccgcgaccgg atcgacgctt tcgtcgaacg gagcgaggtc gacgagatgc agcagcacgc cggtgcgctg caggtgccgc aggaactggt ggccgaggcc cgcgccttcg gccgcgcctt cgatcagccc agggatatcg gcaatcacga agctcttgct cgggccgacc cgcacgacgc ccaggttcgg cgcgagcgtg gtgaacgggt agtcggcgat cttcggcttc gcgttcgaca ccgatgaaat gaaggtcgac ttgccggcgt tcggcatgcc gagcagcccg acgtcggcga gcaccttcag ttcgagccgc agcatgcgcc gctcgccggg cttgccgtcg gtcttctgac gcggcgcccg gttggtgctc gacttgaaat gcaggttgcc gaggccgccg gagccgccct tcgcgagcat cacctgctga tcgtgttcgg tcaggtcggc aatcagctcg ccggtgtcca tgtcggtcac gaccgtgccg accggcatgc gcagcgtgat gtcgtcgccg cccttgccgt aacaatcgga gccgcgaccg ttttcgccgt tgcgcgccat gtgcttcttc gcgtaacggt agtcgatcag cgtgttgatg ttgcggtcgg cgatcgcgta gacgttaccg ccccggccgc cgtcgccgcc gtccggcccg ccgaacggaa cgaacttctc gcggcgcatc gacgcgctgc catcgcctcc gtcgccggcg atgacttcga ttcgtgcttc gtcaatgaac ttcatcgaac caggaccctg ggtctgagct tccagggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGNVYAIA DRNINTLIDY RYAKKHMARN GENGRGSDCY GKGGDDITLR MPVGTVVTDM DTGELIADLT EHDQQVMLAK GGSGGLGNLH FKSSTNRAPR QKTDGKPGER RMLRLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG VLLHLVDLAP FDESVDPVAE AKAIVGELRK YDEALYEKPR WLVLNKLDMV PEDERDARVA DFLERFGWDG PVFQISALTG QGCEALCYAI YDYLAEHSDA HRAAEEEDLA ADVRFRDAPP SDGGATSGGD A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagttcatt gacgaagcac gaatcgaagt catcgccggc gacggaggcg atggcagcgc gtcgatgcgc cgcgagaagt tcgttccgtt cggcgggccg gacggcggcg acggcggccg gggcggtaac gtctacgcga tcgccgaccg caacatcaac acgctgatcg actaccgtta cgcgaagaag cacatggcgc gcaacggcga aaacggtcgc ggctccgatt gttacggcaa gggcggcgac gacatcacgc tgcgcatgcc ggtcggcacg gtcgtgaccg acatggacac cggcgagctg attgccgacc tgaccgaaca cgatcagcag gtgatgctcg cgaagggcgg ctccggcggc ctcggcaacc tgcatttcaa gtcgagcacc aaccgggcgc cgcgtcagaa gaccgacggc aagcccggcg agcggcgcat gctgcggctc gaactgaagg tgctcgccga cgtcgggctg ctcggcatgc cgaacgccgg caagtcgacc ttcatttcat cggtgtcgaa cgcgaagccg aagatcgccg actacccgtt caccacgctc gcgccgaacc tgggcgtcgt gcgggtcggc ccgagcaaga gcttcgtgat tgccgatatc cctgggctga tcgaaggcgc ggccgaaggc gcgggcctcg gccaccagtt cctgcggcac ctgcagcgca ccggcgtgct gctgcatctc gtcgacctcg ctccgttcga cgaaagcgtc gatccggtcg cggaagcgaa ggcgatcgtc ggcgaactgc gcaagtatga cgaggcgctc tacgaaaagc cgcgctggct cgtgctgaac aagctcgaca tggtgccgga agacgagcgc gacgcgcgcg ttgccgactt cctcgagcgt ttcggctggg acggcccggt gttccagatt tcggccctga ccggccaggg ctgcgaagcc ctgtgctatg cgatctacga ttaccttgcc gagcattcgg acgcgcatcg cgcggcggaa gaggaagatc tggccgcgga cgtgcgcttc cgcgatgcgc cgccgagcga cggcggcgca acctcaggcg gcgatgccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuviA.00120.a.A1.PS01327
PURIFICATION DATe: 1/5/2012
CONCENTRATION: 70.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGNVYAIAD RNINTLIDYR YAKKHMARNG ENGRGSDCYG KGGDDITLRM PVGTVVTDMD TGELIADLTE HDQQVMLAKG GSGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DESVDPVAEA KAIVGELRKY DEALYEKPRW LVLNKLDMVP EDERDARVAD FLERFGWDGP VFQISALTGQ GCEALCYAIY DYLAEHSDAH RAAEEEDLAA DVRFRDAPPS DGGATSGG
Validated NT Sequence
cgccgcctga ggttgcgccg ccgtcgctcg gcggcgcatc gcggaagcgc acgtccgcgg ccagatcttc ctcttccgcc gcgcgatgcg cgtccgaatg ctcggcaagg taatcgtaga tcgcatagca cagggcttcg cagccctggc cggtcagggc cgaaatctgg aacaccgggc cgtcccagcc gaaacgctcg aggaagtcgg caacgcgcgc gtcgcgctcg tcttccggca ccatgtcgag cttgttcagc acgagccagc gcggcttttc gtagagcgcc tcgtcatact tgcgcagttc gccgacgatc gccttcgctt ccgcgaccgg atcgacgctt tcgtcgaacg gagcgaggtc gacgagatgc agcagcacgc cggtgcgctg caggtgccgc aggaactggt ggccgaggcc cgcgccttcg gccgcgcctt cgatcagccc agggatatcg gcaatcacga agctcttgct cgggccgacc cgcacgacgc ccaggttcgg cgcgagcgtg gtgaacgggt agtcggcgat cttcggcttc gcgttcgaca ccgatgaaat gaaggtcgac ttgccggcgt tcggcatgcc gagcagcccg acgtcggcga gcaccttcag ttcgagccgc agcatgcgcc gctcgccggg cttgccgtcg gtcttctgac gcggcgcccg gttggtgctc gacttgaaat gcaggttgcc gaggccgccg gagccgccct tcgcgagcat cacctgctga tcgtgttcgg tcaggtcggc aatcagctcg ccggtgtcca tgtcggtcac gaccgtgccg accggcatgc gcagcgtgat gtcgtcgccg cccttgccgt aacaatcgga gccgcgaccg ttttcgccgt tgcgcgccat gtgcttcttc gcgtaacggt agtcgatcag cgtgttgatg ttgcggtcgg cgatcgcgta gacgttaccg ccccggccgc cgtcgccgcc gtccggcccg ccgaacggaa cgaacttctc gcggcgcatc gacgcgctgc catcgcctcc gtcgccggcg atgacttcga ttcgtgcttc gtcaatgaac ttcatcgaac caggaccctg ggtctgagct tccagggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGNVYAIA DRNINTLIDY RYAKKHMARN GENGRGSDCY GKGGDDITLR MPVGTVVTDM DTGELIADLT EHDQQVMLAK GGSGGLGNLH FKSSTNRAPR QKTDGKPGER RMLRLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG VLLHLVDLAP FDESVDPVAE AKAIVGELRK YDEALYEKPR WLVLNKLDMV PEDERDARVA DFLERFGWDG PVFQISALTG QGCEALCYAI YDYLAEHSDA HRAAEEEDLA ADVRFRDAPP SDGGATSGGD A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGTTCATT GACGAAGCAC GAATCGAAGT CATCGCCGGC GACGGAGGCG ATGGCAGCGC GTCGATGCGC CGCGAGAAGT TCGTTCCGTT CGGCGGGCCG GACGGCGGCG ACGGCGGCCG GGGCGGTAAC GTCTACGCGA TCGCCGACCG CAACATCAAC ACGCTGATCG ACTACCGTTA CGCGAAGAAG CACATGGCGC GCAACGGCGA AAACGGTCGC GGCTCCGATT GTTACGGCAA GGGCGGCGAC GACATCACGC TGCGCATGCC GGTCGGCACG GTCGTGACCG ACATGGACAC CGGCGAGCTG ATTGCCGACC TGACCGAACA CGATCAGCAG GTGATGCTCG CGAAGGGCGG CTCCGGCGGC CTCGGCAACC TGCATTTCAA GTCGAGCACC AACCGGGCGC CGCGTCAGAA GACCGACGGC AAGCCCGGCG AGCGGCGCAT GCTGCGGCTC GAACTGAAGG TGCTCGCCGA CGTCGGGCTG CTCGGCATGC CGAACGCCGG CAAGTCGACC TTCATTTCAT CGGTGTCGAA CGCGAAGCCG AAGATCGCCG ACTACCCGTT CACCACGCTC GCGCCGAACC TGGGCGTCGT GCGGGTCGGC CCGAGCAAGA GCTTCGTGAT TGCCGATATC CCTGGGCTGA TCGAAGGCGC GGCCGAAGGC GCGGGCCTCG GCCACCAGTT CCTGCGGCAC CTGCAGCGCA CCGGCGTGCT GCTGCATCTC GTCGACCTCG CTCCGTTCGA CGAAAGCGTC GATCCGGTCG CGGAAGCGAA GGCGATCGTC GGCGAACTGC GCAAGTATGA CGAGGCGCTC TACGAAAAGC CGCGCTGGCT CGTGCTGAAC AAGCTCGACA TGGTGCCGGA AGACGAGCGC GACGCGCGCG TTGCCGACTT CCTCGAGCGT TTCGGCTGGG ACGGCCCGGT GTTCCAGATT TCGGCCCTGA CCGGCCAGGG CTGCGAAGCC CTGTGCTATG CGATCTACGA TTACCTTGCC GAGCATTCGG ACGCGCATCG CGCGGCGGAA GAGGAAGATC TGGCCGCGGA CGTGCGCTTC CGCGATGCGC CGCCGAGCGA CGGCGGCGCA ACCTCAGGCG GCGATGCCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A