BuviA.00119.a

D-alanine--D-alanine ligase

CENTER ID: BuviA.00119.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00119.a.B1.GE35348 Full length( BuviA.00119.a ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4733
RefSeq: YP_001118384.1
UniProt: A4JB96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGIDPKRFG KVAVLFGGES AEREVSLTSG RLVLQGLRDA GIDAHLFDPA ERPLSALKDE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGVPTPP FETVLRGDDY AARATEIVAK LGLPLFVKPA SEGSSVAVLK VKTADALPAA LSEAATHDKI VIVEKSIEGG GEYTACIAGD LDLPLIKIVP AGEFYDYHAK YVADDTQYLI PCGLPAAQEA ELKRIARRAF DVLGCTDWGR ADFMLDAAGN AYFLEVNTAP GMTDHSLPPK AARSIGISYS ELVVKVLSLT LND
NT Sequence
ATGAGCGGGA TCGATCCGAA ACGTTTCGGC AAGGTGGCGG TGTTGTTCGG CGGCGAGTCG GCCGAACGCG AGGTGTCGCT CACCTCGGGC CGCCTCGTGC TGCAGGGCCT GCGCGACGCA GGCATCGACG CGCATCTGTT CGACCCGGCC GAGCGGCCGC TGTCGGCGCT GAAGGACGAA GGCTTCGTGC GCGCGTTCAA CGCGCTGCAC GGCGGCTACG GCGAGAACGG CCAGATCCAG GGCGCGCTCG ACTTCTACGG GATTCGCTAC ACGGGGAGCG GCGTGCTCGG CTCGGCGCTC GGTCTCGACA AGTTCCGCAC CAAGCTCGTG TGGCAGCAGA CGGGCGTGCC GACGCCGCCG TTCGAAACGG TGCTGCGCGG CGACGACTAC GCGGCGCGCG CGACCGAGAT CGTCGCGAAG CTGGGCCTGC CGCTGTTCGT GAAGCCGGCG AGCGAAGGCT CGAGCGTCGC CGTGCTGAAG GTGAAGACGG CCGACGCGTT GCCCGCCGCA CTGTCGGAAG CCGCGACGCA CGACAAGATC GTGATCGTGG AGAAGAGCAT CGAAGGCGGC GGCGAGTACA CCGCGTGCAT CGCCGGCGAT CTCGACCTGC CGCTGATCAA GATCGTGCCG GCCGGCGAGT TCTACGACTA TCACGCGAAG TACGTCGCCG ACGATACGCA GTACCTGATC CCGTGCGGTT TGCCGGCGGC GCAGGAAGCG GAGCTGAAGC GCATCGCGCG GCGCGCGTTC GACGTGCTCG GCTGCACCGA CTGGGGCCGC GCGGACTTCA TGCTGGACGC GGCCGGCAAC GCGTACTTCC TCGAAGTGAA CACGGCGCCC GGGATGACCG ATCATTCGCT GCCGCCGAAG GCCGCACGCT CGATCGGCAT CAGCTATTCG GAGCTGGTCG TGAAGGTGCT GTCGCTTACG CTCAACGAT
Details for BuviA.00119.a.B1.GE35348
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMS GIDPKRFGKV AVLFGGESAE REVSLTSGRL VLQGLRDAGI DAHLFDPAER PLSALKDEGF VRAFNALHGG YGENGQIQGA LDFYGIRYTG SGVLGSALGL DKFRTKLVWQ QTGVPTPPFE TVLRGDDYAA RATEIVAKLG LPLFVKPASE GSSVAVLKVK TADALPAALS EAATHDKIVI VEKSIEGGGE YTACIAGDLD LPLIKIVPAG EFYDYHAKYV ADDTQYLIPC GLPAAXXAEL KRIARRAFDX LGCXDWGRAX FMLXAXGNAY XLEVTRXPGX XIIRCXXLDR XQLXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttnntnnn nttaagaagg aganatacca tggctcacca ccaccaccac catatgagcg ggatcgatcc gaaacgtttc ggcaaggtgg cggtgttgtt cggcggcgag tcggccgaac gcgaggtgtc gctcacctcg ggccgcctcg tgctgcaggg cctgcgcgac gcaggcatcg acgcgcatct gttcgacccg gccgagcggc cgctgtcggc gctgaaggac gaaggcttcg tgcgcgcgtt caacgcgctg cacggcggct acggcgagaa cggccagatc cagggcgcgc tcgacttcta cgggattcgc tacacgggga gcggcgtgct cggctcggcg ctcggtctcg acaagttccg caccaagctc gtgtggcagc agacgggcgt gccgacgccg ccgttcgaaa cggtgctgcg cggcgacgac tacgcggcgc gcgcgaccga gatcgtcgcg aagctgggcc tgccgctgtt cgtgaagccg gcgagcgaag gctcgagcgt cgccgtgctg aaggtgaaga cggccgacgc gttgcccgcc gcactgtcgg aagccgcgac gcacgacaag atcgtgatcg tggagaagag catcgaaggc ggcggcgagt acaccgcgtg catcgccggc gatctcgacc tgccgctgat caagatcgtg ccggccggcg agttctacga ctatcacgcg aagtacgtcg ccgacgatac gcagtacctg atcccgtgcg gtttgccggc ggcgcannaa gcggagctga agcgcatcgc gcggcgcgcg ttcgacntgc tcggctgcnc cgactggggc cgcgcggnct tcatgctgga ngcngncggc aacgcgtact tnctcgaagt gacncggnnn ccnggnngac ngatcattcg ctgccnncna ctcgatcggn ntcagctnnt cnnancngnn nnnnannngc ngnnnnntnc nnnnnnnnnn nngagnannn nnnncnnnnn nnannnnnnn ncc
Expected Protein Sequence
MAHHHHHHMS GIDPKRFGKV AVLFGGESAE REVSLTSGRL VLQGLRDAGI DAHLFDPAER PLSALKDEGF VRAFNALHGG YGENGQIQGA LDFYGIRYTG SGVLGSALGL DKFRTKLVWQ QTGVPTPPFE TVLRGDDYAA RATEIVAKLG LPLFVKPASE GSSVAVLKVK TADALPAALS EAATHDKIVI VEKSIEGGGE YTACIAGDLD LPLIKIVPAG EFYDYHAKYV ADDTQYLIPC GLPAAQEAEL KRIARRAFDV LGCTDWGRAD FMLDAAGNAY FLEVNTAPGM TDHSLPPKAA RSIGISYSEL VVKVLSLTLN D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ggatcgatcc gaaacgtttc ggcaaggtgg cggtgttgtt cggcggcgag tcggccgaac gcgaggtgtc gctcacctcg ggccgcctcg tgctgcaggg cctgcgcgac gcaggcatcg acgcgcatct gttcgacccg gccgagcggc cgctgtcggc gctgaaggac gaaggcttcg tgcgcgcgtt caacgcgctg cacggcggct acggcgagaa cggccagatc cagggcgcgc tcgacttcta cgggattcgc tacacgggga gcggcgtgct cggctcggcg ctcggtctcg acaagttccg caccaagctc gtgtggcagc agacgggcgt gccgacgccg ccgttcgaaa cggtgctgcg cggcgacgac tacgcggcgc gcgcgaccga gatcgtcgcg aagctgggcc tgccgctgtt cgtgaagccg gcgagcgaag gctcgagcgt cgccgtgctg aaggtgaaga cggccgacgc gttgcccgcc gcactgtcgg aagccgcgac gcacgacaag atcgtgatcg tggagaagag catcgaaggc ggcggcgagt acaccgcgtg catcgccggc gatctcgacc tgccgctgat caagatcgtg ccggccggcg agttctacga ctatcacgcg aagtacgtcg ccgacgatac gcagtacctg atcccgtgcg gtttgccggc ggcgcaggaa gcggagctga agcgcatcgc gcggcgcgcg ttcgacgtgc tcggctgcac cgactggggc cgcgcggact tcatgctgga cgcggccggc aacgcgtact tcctcgaagt gaacacggcg cccgggatga ccgatcattc gctgccgccg aaggccgcac gctcgatcgg catcagctat tcggagctgg tcgtgaaggt gctgtcgctt acgctcaacg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat