BuviA.00118.e
UDP-glucose pyrophosphorylase
| CENTER ID: | BuviA.00118.e |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00118.e.B1.GE39156 | Full length( BuviA.00118.e ) | 1 | 294 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5I1F
DEPOSITED: 2/5/2016
DETERMINATION: XRay
CLONE: BuviA.00118.e.B1.GE39156
PROTEIN: BuviA.00118.e.B1.PS02498
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131936 |
| PATRIC ID: | fig|269482.11.peg.1879 |
| RefSeq: | YP_001115660.1 |
| UniProt: | A4JT02 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAIEAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLSLV RSIKPSHVDC FYVRQAEALG LGHAVLCAEK LVGDNPFAVI LADDLLDGPT PVLRQMIDVF DHYHASVIGV EEIAPADSKS YGVIDGKRWE DDLFKLSGIV EKPEPAQAPS NFGVVGRYVL KPKIFKHLRG LKPGAGGELQ LTDAIQSLLT DEQVLAYRYD GTRFDCGSKL GYLKATVEFA LRHPEVAADF ERYLLARMSE QLVV
NT Sequence
ATGTTGAAAG TGACGAAAGC CGTCTTTCCG GTCGCCGGCC TGGGCACGCG GTTCCTGCCC GCCACCAAGG CGAGCCCCAA GGAAATGTTG CCGGTCGTGG ACAAGCCGTT GATCCAGTAT GCCGTCGAAG AGGCGATCGA AGCGGGCATC ACCGAGATGA TCTTCGTGAC CGGGCGCAGC AAGCGCGCGA TCGAAGATCA CTTCGACAAG TCGTACGAGA TCGAGGCCGA ACTCGAGGCA CGCGGCAAGG AGAAGCTGCT GTCGCTCGTG CGCAGCATCA AGCCGAGCCA CGTCGACTGC TTCTACGTTC GCCAGGCCGA AGCGCTCGGG CTCGGTCACG CCGTCCTGTG CGCGGAGAAG CTCGTCGGCG ACAACCCGTT CGCCGTGATT CTCGCCGACG ACCTGCTCGA CGGCCCGACG CCCGTTCTGC GCCAGATGAT CGACGTATTC GACCACTATC ATGCGTCGGT CATCGGCGTC GAGGAGATCG CGCCGGCGGA TTCGAAGTCC TATGGCGTGA TCGACGGCAA GCGCTGGGAA GACGATCTCT TCAAGCTGTC GGGGATCGTC GAGAAGCCCG AACCGGCGCA GGCGCCGTCG AATTTCGGCG TCGTGGGCCG TTATGTGCTG AAGCCGAAGA TCTTCAAGCA TCTGCGCGGG CTCAAGCCGG GCGCGGGCGG TGAACTGCAG CTGACCGACG CGATCCAGTC GCTGCTCACC GATGAACAGG TGCTCGCGTA CCGCTACGAC GGCACGCGCT TCGACTGCGG CAGCAAGCTC GGCTACCTGA AGGCGACGGT CGAATTTGCG TTGCGGCATC CGGAGGTCGC GGCGGATTTC GAACGGTATC TGCTCGCGCG GATGTCGGAG CAGTTGGTCG TC
Details for BuviA.00118.e.B1.GE39156
| HARVESTED ON: | 5/6/2015 |
| SEQUENCED ON: | 5/6/2015 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIEAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLSLVRS IKPSHVDCFY VRQAEALGLG HAVLCAEKLV GDNPFAVILA DDLLDGPTPV LRQMIDVFDH YHASVIGVEE IAPADSKSYG VIDGKRWEDD LFKLSGIVEK PEPAQAPSNF GVVGRYVLKP KIFKHLRGLK PGAGGELQLT DAIQSLLTDE QVLAYRYDGT RFDCGSKLGY LKATVEFALR HPEVAADFER YLLARMSEQL VV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtgacgaa agccgtcttt ccggtcgccg gcctgggcac gcggttcctg cccgccacca aggcgagccc caaggaaatg ttgccggtcg tggacaagcc gttgatccag tatgccgtcg aagaggcgat cgaagcgggc atcaccgaga tgatcttcgt gaccgggcgc agcaagcgcg cgatcgaaga tcacttcgac aagtcgtacg agatcgaggc cgaactcgag gcacgcggca aggagaagct gctgtcgctc gtgcgcagca tcaagccgag ccacgtcgac tgcttctacg ttcgccaggc cgaagcgctc gggctcggtc acgccgtcct gtgcgcggag aagctcgtcg gcgacaaccc gttcgccgtg attctcgccg acgacctgct cgacggcccg acgcccgttc tgcgccagat gatcgacgta ttcgaccact atcatgcgtc ggtcatcggc gtcgaggaga tcgcgccggc ggattcgaag tcctatggcg tgatcgacgg caagcgctgg gaagacgatc tcttcaagct gtcggggatc gtcgagaagc ccgaaccggc gcaggcgccg tcgaatttcg gcgtcgtggg ccgttatgtg ctgaagccga agatcttcaa gcatctgcgc gggctcaagc cgggcgcggg cggtgaactg cagctgaccg acgcgatcca gtcgctgctc accgatgaac aggtgctcgc gtaccgctac gacggcacgc gcttcgactg cggcagcaag ctcggctacc tgaaggcgac ggtcgaattt gcgttgcggc atccggaggt cgcggcggat ttcgaacggt atctgctcgc gcggatgtcg gagcagttgg tcgtctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat