SSGCID: Target Page

BuviA.00118.d

UDP-glucose pyrophosphorylase

CENTER ID:	BuviA.00118.d
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00118.d.B1.GE39155	Full length( BuviA.00118.d )		1	295

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.3196
RefSeq:	YP_001116909.1
UniProt:	A4JME1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAINAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLELV RGIKPSNVDC FYVRQPEALG LGHAVLCAEK LVHGEPFAVI LADDLLHGEQ PVLKQLVDVF DHYHSSVIGV ETIAREDSRS YGVVEGREWE EDIIKLAGII EKPAPEDAPS NLGVVGRYVF MPTIFDHLRR IKPGAGGELQ LTDAVQSLLA NEQVLAYRYY GTRFDCGSKL GYLKATVEFA LQHPEVSREF EAYLRTCLPA LAAVA

NT Sequence

ATGTTGAAAG TCACCAAGGC CGTGTTCCCC GTTGCCGGTC TCGGCACGCG GTTCCTCCCA GCAACGAAGG CGAGCCCGAA GGAAATGCTG CCTGTCGTGG ACAAGCCGCT GATCCAGTAC GCGGTCGAGG AAGCGATCAA CGCAGGTATC ACGGAGATGA TCTTCGTGAC GGGGCGCAGC AAGCGCGCGA TCGAGGATCA TTTCGACAAG TCATACGAAA TCGAGGCCGA ACTCGAGGCG CGCGGCAAGG AAAAGCTGCT CGAGCTCGTG CGCGGCATCA AGCCGAGCAA CGTGGACTGC TTCTACGTGC GTCAGCCGGA AGCGCTCGGG CTCGGCCATG CGGTGCTGTG CGCGGAAAAG CTCGTGCACG GCGAGCCGTT CGCGGTGATT CTCGCGGACG ATCTGCTGCA CGGCGAGCAG CCGGTGCTCA AGCAGCTCGT CGACGTGTTC GACCACTACC ACAGCTCGGT GATCGGGGTC GAGACGATCG CGCGCGAGGA CAGCCGCTCG TACGGCGTCG TCGAGGGCCG CGAATGGGAA GAGGACATCA TCAAGCTGGC GGGCATCATC GAGAAGCCGG CGCCGGAAGA TGCGCCGTCG AACCTCGGCG TGGTCGGCCG CTACGTGTTC ATGCCGACGA TCTTCGATCA TCTGCGCCGG ATCAAGCCGG GCGCGGGCGG CGAGCTGCAG CTCACCGACG CCGTGCAGTC GCTGCTCGCG AACGAACAGG TGCTCGCGTA CCGCTACTAC GGCACGCGTT TCGATTGCGG CAGCAAGCTC GGCTACCTGA AGGCAACCGT CGAGTTCGCG CTTCAGCATC CGGAAGTGAG CCGCGAATTC GAGGCGTATC TGCGTACCTG TCTGCCGGCG CTCGCTGCCG TCGCA

Details for BuviA.00118.d.B1.GE39155

HARVESTED ON:	5/6/2015
SEQUENCED ON:	5/6/2015
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAINAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLELVRG IKPSNVDCFY VRQPEALGLG HAVLCAEKLV HGEPFAVILA DDLLHGEQPV LKQLVDVFDH YHSSVIGVET IAREDSRSYG VVEGREWEED IIKLAGIIEK PAPEDAPSNL GVVGRYVFMP TIFDHLRRIK PGAGGELQLT DAVQSLLANE QVLAYRYYGT RFDCGSKLGY LKATVEFALQ HPEVSREFEA YLRTCLPALA AVA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaccaa ggccgtgttc cccgttgccg gtctcggcac gcggttcctc ccagcaacga aggcgagccc gaaggaaatg ctgcctgtcg tggacaagcc gctgatccag tacgcggtcg aggaagcgat caacgcaggt atcacggaga tgatcttcgt gacggggcgc agcaagcgcg cgatcgagga tcatttcgac aagtcatacg aaatcgaggc cgaactcgag gcgcgcggca aggaaaagct gctcgagctc gtgcgcggca tcaagccgag caacgtggac tgcttctacg tgcgtcagcc ggaagcgctc gggctcggcc atgcggtgct gtgcgcggaa aagctcgtgc acggcgagcc gttcgcggtg attctcgcgg acgatctgct gcacggcgag cagccggtgc tcaagcagct cgtcgacgtg ttcgaccact accacagctc ggtgatcggg gtcgagacga tcgcgcgcga ggacagccgc tcgtacggcg tcgtcgaggg ccgcgaatgg gaagaggaca tcatcaagct ggcgggcatc atcgagaagc cggcgccgga agatgcgccg tcgaacctcg gcgtggtcgg ccgctacgtg ttcatgccga cgatcttcga tcatctgcgc cggatcaagc cgggcgcggg cggcgagctg cagctcaccg acgccgtgca gtcgctgctc gcgaacgaac aggtgctcgc gtaccgctac tacggcacgc gtttcgattg cggcagcaag ctcggctacc tgaaggcaac cgtcgagttc gcgcttcagc atccggaagt gagccgcgaa ttcgaggcgt atctgcgtac ctgtctgccg gcgctcgctg ccgtcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat