BuviA.00118.c
Glucose-1-phosphate thymidylyltransferase
| CENTER ID: | BuviA.00118.c |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00118.c.B1.GE39154 | Full length( BuviA.00118.c ) | 1 | 294 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00118.c.B1.PS02537 | Full length( BuviA.00118.c ) | 1 | 294 |
Structures
5IDS
DEPOSITED: 2/24/2016
DETERMINATION: XRay
CLONE: BuviA.00118.c.B1.GE39154
PROTEIN: BuviA.00118.c.B1.PS02537
5IDT
DEPOSITED: 2/24/2016
DETERMINATION: XRay
CLONE: BuviA.00118.c.B1.GE39154
PROTEIN: BuviA.00118.c.B1.PS02537
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131815 |
| PATRIC ID: | fig|269482.11.peg.7502 |
| RefSeq: | YP_001121040.1 |
| UniProt: | A4JIV2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQRKGIILA GGSGTRLHPA TLAISKQLLP VYDKPMIYYP LSTLMLAGMR DVLVISTPQD TPRFQQLLGD GSQWGMNLQY AVQPSPDGLA QAFIIGEQFI GNAPSALVLG DNIYYGHDFQ PLLKAADAQS SGATVFAYHV HDPERYGVVQ FNAQGQAVSI EEKPKAPKSN YAVTGLYFYD QQVVDIAKAV KPSARGELEI TSVNQAYMQQ GQLNVQTMGR GYAWLDTGTH DSLLDASQFI ATLENRQGLK VACPEEIAWR SGWINASQLE ALVQPLTKNG YGQYLMQILK ETVF
NT Sequence
ATGACCCAAC GAAAAGGCAT CATCCTCGCC GGAGGCTCCG GCACCCGGCT GCACCCGGCC ACGCTGGCCA TCAGCAAACA ACTGCTGCCG GTGTACGACA AGCCTATGAT CTATTACCCG CTGAGCACCC TCATGCTTGC GGGCATGCGA GATGTGCTCG TGATCAGCAC GCCGCAAGAC ACCCCCCGCT TCCAGCAACT GCTGGGTGAC GGCTCGCAGT GGGGCATGAA CCTCCAGTAT GCCGTGCAAC CCAGCCCCGA CGGCTTGGCG CAGGCCTTCA TCATTGGTGA ACAGTTCATT GGCAACGCTC CCAGCGCGCT GGTGCTGGGC GACAACATCT ACTACGGGCA CGACTTCCAG CCCCTGCTCA AAGCGGCCGA TGCGCAATCG AGCGGCGCCA CCGTGTTTGC CTACCACGTA CATGACCCCG AGCGCTATGG CGTGGTCCAG TTCAACGCCC AGGGTCAGGC CGTCAGCATC GAAGAAAAGC CCAAGGCCCC AAAGAGCAAC TACGCCGTCA CCGGTCTGTA CTTTTACGAC CAGCAGGTGG TGGACATTGC CAAGGCCGTG AAGCCCAGCG CGCGGGGCGA GCTGGAAATC ACCAGCGTCA ATCAGGCCTA CATGCAGCAA GGCCAGTTGA ACGTGCAAAC CATGGGCCGT GGCTACGCCT GGCTGGACAC CGGCACCCAC GACAGCCTGC TCGATGCCAG CCAGTTCATT GCAACCCTAG AAAACCGCCA AGGCCTCAAA GTGGCCTGCC CGGAGGAAAT TGCCTGGCGT AGCGGCTGGA TCAACGCCAG CCAGCTCGAA GCCCTAGTCC AGCCGCTTAC CAAAAATGGC TACGGCCAAT ACCTGATGCA GATCTTGAAA GAGACCGTCT TC
Details for BuviA.00118.c.B1.GE39154
| HARVESTED ON: | 5/6/2015 |
| SEQUENCED ON: | 5/6/2015 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMT QRKGIILAGG SGTRLHPATL AISKQLLPVY DKPMIYYPLS TLMLAGMRDV LVISTPQDTP RFQQLLGDGS QWGMNLQYAV QPSPDGLAQA FIIGEQFIGN APSALVLGDN IYYGHDFQPL LKAADAQSSG ATVFAYHVHD PERYGVVQFN AQGQAVSIEE KPKAPKSNYA VTGLYFYDQQ VVDIAKAVKP SARGELEITS VNQAYMQQGQ LNVQTMGRGY AWLDTGTHDS LLDASQFIAT LENRQGLKVA CPEEIAWRSG WINASQLEAL VQPLTKNGYG QY
Validated NT Sequence
aggtattggc cgtagccatt tttggtaagc ggctggacta gggcttcgag ctggctggcg ttgatccagc cgctacgcca ggcaatttcc tccgggcagg ccactttgag gccttggcgg ttttctaggg ttgcaatgaa ctggctggca tcgagcaggc tgtcgtgggt gccggtgtcc agccaggcgt agccacggcc catggtttgc acgttcaact ggccttgctg catgtaggcc tgattgacgc tggtgatttc cagctcgccc cgcgcgctgg gcttcacggc cttggcaatg tccaccacct gctggtcgta aaagtacaga ccggtgacgg cgtagttgct ctttggggcc ttgggctttt cttcgatgct gacggcctga ccctgggcgt tgaactggac cacgccatag cgctcggggt catgtacgtg gtaggcaaac acggtggcgc cgctcgattg cgcatcggcc gctttgagca ggggctggaa gtcgtgcccg tagtagatgt tgtcgcccag caccagcgcg ctgggagcgt tgccaatgaa ctgttcacca atgatgaagg cctgcgccaa gccgtcgggg ctgggttgca cggcatactg gaggttcatg ccccactgcg agccgtcacc cagcagttgc tggaagcggg gggtgtcttg cggcgtgctg atcacgagca catctcgcat gcccgcaagc atgagggtgc tcagcgggta atagatcata ggcttgtcgt acaccggcag cagttgtttg ctgatggcca gcgtggccgg gtgcagccgg gtgccggagc ctccggcgag gatgatgcct tttcgttggg tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMT QRKGIILAGG SGTRLHPATL AISKQLLPVY DKPMIYYPLS TLMLAGMRDV LVISTPQDTP RFQQLLGDGS QWGMNLQYAV QPSPDGLAQA FIIGEQFIGN APSALVLGDN IYYGHDFQPL LKAADAQSSG ATVFAYHVHD PERYGVVQFN AQGQAVSIEE KPKAPKSNYA VTGLYFYDQQ VVDIAKAVKP SARGELEITS VNQAYMQQGQ LNVQTMGRGY AWLDTGTHDS LLDASQFIAT LENRQGLKVA CPEEIAWRSG WINASQLEAL VQPLTKNGYG QYLMQILKET VF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aacgaaaagg catcatcctc gccggaggct ccggcacccg gctgcacccg gccacgctgg ccatcagcaa acaactgctg ccggtgtacg acaagcctat gatctattac ccgctgagca ccctcatgct tgcgggcatg cgagatgtgc tcgtgatcag cacgccgcaa gacacccccc gcttccagca actgctgggt gacggctcgc agtggggcat gaacctccag tatgccgtgc aacccagccc cgacggcttg gcgcaggcct tcatcattgg tgaacagttc attggcaacg ctcccagcgc gctggtgctg ggcgacaaca tctactacgg gcacgacttc cagcccctgc tcaaagcggc cgatgcgcaa tcgagcggcg ccaccgtgtt tgcctaccac gtacatgacc ccgagcgcta tggcgtggtc cagttcaacg cccagggtca ggccgtcagc atcgaagaaa agcccaaggc cccaaagagc aactacgccg tcaccggtct gtacttttac gaccagcagg tggtggacat tgccaaggcc gtgaagccca gcgcgcgggg cgagctggaa atcaccagcg tcaatcaggc ctacatgcag caaggccagt tgaacgtgca aaccatgggc cgtggctacg cctggctgga caccggcacc cacgacagcc tgctcgatgc cagccagttc attgcaaccc tagaaaaccg ccaaggcctc aaagtggcct gcccggagga aattgcctgg cgtagcggct ggatcaacgc cagccagctc gaagccctag tccagccgct taccaaaaat ggctacggcc aatacctgat gcagatcttg aaagagaccg tcttctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuviA.00118.c.B1.PS02537
| PURIFICATION DATe: | 11/11/2015 |
| CONCENTRATION: | 61.46mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QRKGIILAGG SGTRLHPATL AISKQLLPVY DKPMIYYPLS TLMLAGMRDV LVISTPQDTP RFQQLLGDGS QWGMNLQYAV QPSPDGLAQA FIIGEQFIGN APSALVLGDN IYYGHDFQPL LKAADAQSSG ATVFAYHVHD PERYGVVQFN AQGQAVSIEE KPKAPKSNYA VTGLYFYDQQ VVDIAKAVKP SARGELEITS VNQAYMQQGQ LNVQTMGRGY AWLDTGTHDS LLDASQFIAT LENRQGLKVA CPEEIAWRSG WINASQLEAL VQPLTKNGYG QY
Validated NT Sequence
aggtattggc cgtagccatt tttggtaagc ggctggacta gggcttcgag ctggctggcg ttgatccagc cgctacgcca ggcaatttcc tccgggcagg ccactttgag gccttggcgg ttttctaggg ttgcaatgaa ctggctggca tcgagcaggc tgtcgtgggt gccggtgtcc agccaggcgt agccacggcc catggtttgc acgttcaact ggccttgctg catgtaggcc tgattgacgc tggtgatttc cagctcgccc cgcgcgctgg gcttcacggc cttggcaatg tccaccacct gctggtcgta aaagtacaga ccggtgacgg cgtagttgct ctttggggcc ttgggctttt cttcgatgct gacggcctga ccctgggcgt tgaactggac cacgccatag cgctcggggt catgtacgtg gtaggcaaac acggtggcgc cgctcgattg cgcatcggcc gctttgagca ggggctggaa gtcgtgcccg tagtagatgt tgtcgcccag caccagcgcg ctgggagcgt tgccaatgaa ctgttcacca atgatgaagg cctgcgccaa gccgtcgggg ctgggttgca cggcatactg gaggttcatg ccccactgcg agccgtcacc cagcagttgc tggaagcggg gggtgtcttg cggcgtgctg atcacgagca catctcgcat gcccgcaagc atgagggtgc tcagcgggta atagatcata ggcttgtcgt acaccggcag cagttgtttg ctgatggcca gcgtggccgg gtgcagccgg gtgccggagc ctccggcgag gatgatgcct tttcgttggg tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMT QRKGIILAGG SGTRLHPATL AISKQLLPVY DKPMIYYPLS TLMLAGMRDV LVISTPQDTP RFQQLLGDGS QWGMNLQYAV QPSPDGLAQA FIIGEQFIGN APSALVLGDN IYYGHDFQPL LKAADAQSSG ATVFAYHVHD PERYGVVQFN AQGQAVSIEE KPKAPKSNYA VTGLYFYDQQ VVDIAKAVKP SARGELEITS VNQAYMQQGQ LNVQTMGRGY AWLDTGTHDS LLDASQFIAT LENRQGLKVA CPEEIAWRSG WINASQLEAL VQPLTKNGYG QYLMQILKET VF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AACGAAAAGG CATCATCCTC GCCGGAGGCT CCGGCACCCG GCTGCACCCG GCCACGCTGG CCATCAGCAA ACAACTGCTG CCGGTGTACG ACAAGCCTAT GATCTATTAC CCGCTGAGCA CCCTCATGCT TGCGGGCATG CGAGATGTGC TCGTGATCAG CACGCCGCAA GACACCCCCC GCTTCCAGCA ACTGCTGGGT GACGGCTCGC AGTGGGGCAT GAACCTCCAG TATGCCGTGC AACCCAGCCC CGACGGCTTG GCGCAGGCCT TCATCATTGG TGAACAGTTC ATTGGCAACG CTCCCAGCGC GCTGGTGCTG GGCGACAACA TCTACTACGG GCACGACTTC CAGCCCCTGC TCAAAGCGGC CGATGCGCAA TCGAGCGGCG CCACCGTGTT TGCCTACCAC GTACATGACC CCGAGCGCTA TGGCGTGGTC CAGTTCAACG CCCAGGGTCA GGCCGTCAGC ATCGAAGAAA AGCCCAAGGC CCCAAAGAGC AACTACGCCG TCACCGGTCT GTACTTTTAC GACCAGCAGG TGGTGGACAT TGCCAAGGCC GTGAAGCCCA GCGCGCGGGG CGAGCTGGAA ATCACCAGCG TCAATCAGGC CTACATGCAG CAAGGCCAGT TGAACGTGCA AACCATGGGC CGTGGCTACG CCTGGCTGGA CACCGGCACC CACGACAGCC TGCTCGATGC CAGCCAGTTC ATTGCAACCC TAGAAAACCG CCAAGGCCTC AAAGTGGCCT GCCCGGAGGA AATTGCCTGG CGTAGCGGCT GGATCAACGC CAGCCAGCTC GAAGCCCTAG TCCAGCCGCT TACCAAAAAT GGCTACGGCC AATACCTGAT GCAGATCTTG AAAGAGACCG TCTTCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT