BuviA.00118.b

UDP-glucose pyrophosphorylase

CENTER ID: BuviA.00118.b
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00118.b.B1.GE39153 Full length( BuviA.00118.b ) 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00118.b.B1.PS02488 Full length( BuviA.00118.b ) 1 294

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.5633
RefSeq: YP_001119245.1
UniProt: A4JDQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAIAAGI TEMIFVTGRS KRAIEDHFDK SYEVEAELEA RGKEKLLELV RSIKPSHVDC FYVRQPEALG LGHAVLCAEK LVADNPFAVI LADDLLDGNP PVMKQMVDVF DHYHSSVIGV EEIPPSETKS YGIVDGKEWE ESIVKMSAIV EKPAPEDAPS NLGVVGRYIL KPRIFQHLRA LKPGAGGELQ LTDAIQALLA DEQVLAYKYQ GTRYDCGSKL GYLKATVEFA LRHPEVGAEF EAYLRTRGTA QPAA
NT Sequence
ATGTTGAAAG TCACCAAGGC GGTATTCCCC GTTGCCGGTC TCGGCACGCG GTTCCTGCCC GCAACGAAGG CGAGCCCGAA GGAAATGCTG CCCGTCGTCG ACAAGCCGCT GATCCAGTAC GCCGTGGAGG AAGCGATCGC CGCGGGCATC ACCGAGATGA TCTTCGTGAC GGGGCGCAGC AAGCGCGCGA TCGAGGACCA CTTCGACAAG TCGTACGAGG TCGAGGCCGA ACTCGAGGCG CGCGGCAAGG AAAAGCTGCT GGAGCTCGTG CGCAGCATCA AGCCGAGCCA CGTCGACTGC TTCTACGTGC GTCAGCCGGA AGCGCTCGGC CTCGGCCACG CGGTGCTCTG CGCGGAAAAG CTGGTCGCCG ACAATCCGTT CGCGGTGATC CTCGCCGACG ACCTGCTCGA CGGCAACCCG CCGGTGATGA AGCAGATGGT CGACGTGTTC GACCACTATC ACAGCTCGGT GATCGGCGTC GAAGAGATCC CGCCGTCGGA GACGAAATCG TATGGGATCG TCGACGGCAA GGAGTGGGAA GAGTCGATCG TCAAGATGTC GGCGATCGTC GAGAAGCCGG CGCCGGAAGA CGCGCCGTCG AACCTCGGCG TGGTCGGCCG CTACATCCTG AAGCCGCGGA TCTTCCAGCA TCTGCGGGCG CTGAAGCCGG GCGCGGGCGG CGAGCTGCAG CTCACCGACG CGATCCAGGC GCTGCTCGCC GACGAGCAGG TGCTGGCCTA CAAGTATCAG GGCACGCGCT ACGACTGCGG CAGCAAGCTC GGCTACCTGA AGGCGACCGT CGAATTCGCA CTGCGCCACC CGGAAGTCGG CGCGGAGTTC GAAGCGTACC TGCGCACGCG CGGCACCGCG CAACCGGCCG CC
Details for BuviA.00118.b.B1.GE39153
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KEKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEDAPSNL GVVGRYILKP RIFQHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGAEFEA YLRTXXXAQP AA
Validated NT Sequence
atcttactca cttaggcggc cggttgcgcg nnnnnnnncg tgcgcaggta cgcttcgaac tccgcgccga cttccgggtg gcgcagtgcg aattcgacgg tcgccttcag gtagccgagc ttgctgccgc agtcgtagcg cgtgccctga tacttgtagg ccagcacctg ctcgtcggcg agcagcgcct ggatcgcgtc ggtgagctgc agctcgccgc ccgcgcccgg cttcagcgcc cgcagatgct ggaagatccg cggcttcagg atgtagcggc cgaccacgcc gaggttcgac ggcgcgtctt ccggcgccgg cttctcgacg atcgccgaca tcttgacgat cgactcttcc cactccttgc cgtcgacgat cccatacgat ttcgtctccg acggcgggat ctcttcgacg ccgatcaccg agctgtgata gtggtcgaac acgtcgacca tctgcttcat caccggcggg ttgccgtcga gcaggtcgtc ggcgaggatc accgcgaacg gattgtcggc gaccagcttt tccgcgcaga gcaccgcgtg gccgaggccg agcgcttccg gctgacgcac gtagaagcag tcgacgtggc tcggcttgat gctgcgcacg agctccagca gcttttcctt gccgcgcgcc tcgagttcgg cctcgacctc gtacgacttg tcgaagtggt cctcgatcgc gcgcttgctg cgccccgtca cgaagatcat ctcggtgatg cccgcggcga tcgcttcctc cacggcgtac tggatcagcg gcttgtcgac gacgggcagc atttccttcg ggctcgcctt cgttgcgggc aggaaccgcg tgccgagacc ggcaacgggg aataccgcct tggtgacttt caacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KEKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEDAPSNL GVVGRYILKP RIFQHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGAEFEA YLRTRGTAQP AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaccaa ggcggtattc cccgttgccg gtctcggcac gcggttcctg cccgcaacga aggcgagccc gaaggaaatg ctgcccgtcg tcgacaagcc gctgatccag tacgccgtgg aggaagcgat cgccgcgggc atcaccgaga tgatcttcgt gacggggcgc agcaagcgcg cgatcgagga ccacttcgac aagtcgtacg aggtcgaggc cgaactcgag gcgcgcggca aggaaaagct gctggagctc gtgcgcagca tcaagccgag ccacgtcgac tgcttctacg tgcgtcagcc ggaagcgctc ggcctcggcc acgcggtgct ctgcgcggaa aagctggtcg ccgacaatcc gttcgcggtg atcctcgccg acgacctgct cgacggcaac ccgccggtga tgaagcagat ggtcgacgtg ttcgaccact atcacagctc ggtgatcggc gtcgaagaga tcccgccgtc ggagacgaaa tcgtatggga tcgtcgacgg caaggagtgg gaagagtcga tcgtcaagat gtcggcgatc gtcgagaagc cggcgccgga agacgcgccg tcgaacctcg gcgtggtcgg ccgctacatc ctgaagccgc ggatcttcca gcatctgcgg gcgctgaagc cgggcgcggg cggcgagctg cagctcaccg acgcgatcca ggcgctgctc gccgacgagc aggtgctggc ctacaagtat cagggcacgc gctacgactg cggcagcaag ctcggctacc tgaaggcgac cgtcgaattc gcactgcgcc acccggaagt cggcgcggag ttcgaagcgt acctgcgcac gcgcggcacc gcgcaaccgg ccgcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuviA.00118.b.B1.PS02488
PURIFICATION DATe: 9/3/2015
CONCENTRATION: 29.95mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KEKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEDAPSNL GVVGRYILKP RIFQHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGAEFEA YLRTXXXAQP AA
Validated NT Sequence
atcttactca cttaggcggc cggttgcgcg nnnnnnnncg tgcgcaggta cgcttcgaac tccgcgccga cttccgggtg gcgcagtgcg aattcgacgg tcgccttcag gtagccgagc ttgctgccgc agtcgtagcg cgtgccctga tacttgtagg ccagcacctg ctcgtcggcg agcagcgcct ggatcgcgtc ggtgagctgc agctcgccgc ccgcgcccgg cttcagcgcc cgcagatgct ggaagatccg cggcttcagg atgtagcggc cgaccacgcc gaggttcgac ggcgcgtctt ccggcgccgg cttctcgacg atcgccgaca tcttgacgat cgactcttcc cactccttgc cgtcgacgat cccatacgat ttcgtctccg acggcgggat ctcttcgacg ccgatcaccg agctgtgata gtggtcgaac acgtcgacca tctgcttcat caccggcggg ttgccgtcga gcaggtcgtc ggcgaggatc accgcgaacg gattgtcggc gaccagcttt tccgcgcaga gcaccgcgtg gccgaggccg agcgcttccg gctgacgcac gtagaagcag tcgacgtggc tcggcttgat gctgcgcacg agctccagca gcttttcctt gccgcgcgcc tcgagttcgg cctcgacctc gtacgacttg tcgaagtggt cctcgatcgc gcgcttgctg cgccccgtca cgaagatcat ctcggtgatg cccgcggcga tcgcttcctc cacggcgtac tggatcagcg gcttgtcgac gacgggcagc atttccttcg ggctcgcctt cgttgcgggc aggaaccgcg tgccgagacc ggcaacgggg aataccgcct tggtgacttt caacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KEKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEDAPSNL GVVGRYILKP RIFQHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGAEFEA YLRTRGTAQP AA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA AAGTCACCAA GGCGGTATTC CCCGTTGCCG GTCTCGGCAC GCGGTTCCTG CCCGCAACGA AGGCGAGCCC GAAGGAAATG CTGCCCGTCG TCGACAAGCC GCTGATCCAG TACGCCGTGG AGGAAGCGAT CGCCGCGGGC ATCACCGAGA TGATCTTCGT GACGGGGCGC AGCAAGCGCG CGATCGAGGA CCACTTCGAC AAGTCGTACG AGGTCGAGGC CGAACTCGAG GCGCGCGGCA AGGAAAAGCT GCTGGAGCTC GTGCGCAGCA TCAAGCCGAG CCACGTCGAC TGCTTCTACG TGCGTCAGCC GGAAGCGCTC GGCCTCGGCC ACGCGGTGCT CTGCGCGGAA AAGCTGGTCG CCGACAATCC GTTCGCGGTG ATCCTCGCCG ACGACCTGCT CGACGGCAAC CCGCCGGTGA TGAAGCAGAT GGTCGACGTG TTCGACCACT ATCACAGCTC GGTGATCGGC GTCGAAGAGA TCCCGCCGTC GGAGACGAAA TCGTATGGGA TCGTCGACGG CAAGGAGTGG GAAGAGTCGA TCGTCAAGAT GTCGGCGATC GTCGAGAAGC CGGCGCCGGA AGACGCGCCG TCGAACCTCG GCGTGGTCGG CCGCTACATC CTGAAGCCGC GGATCTTCCA GCATCTGCGG GCGCTGAAGC CGGGCGCGGG CGGCGAGCTG CAGCTCACCG ACGCGATCCA GGCGCTGCTC GCCGACGAGC AGGTGCTGGC CTACAAGTAT CAGGGCACGC GCTACGACTG CGGCAGCAAG CTCGGCTACC TGAAGGCGAC CGTCGAATTC GCACTGCGCC ACCCGGAAGT CGGCGCGGAG TTCGAAGCGT ACCTGCGCAC GCGCGGCACC GCGCAACCGG CCGCCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT