BuviA.00118.a
Glucose-1-phosphate thymidylyltransferase
| CENTER ID: | BuviA.00118.a |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00118.a.B1.GE39152 | Full length( BuviA.00118.a ) | 1 | 297 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5IFY
DEPOSITED: 2/26/2016
DETERMINATION: XRay
CLONE: BuviA.00118.a.B1.GE39152
PROTEIN: BuviA.00118.a.B1.PS02510
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|269482.11.peg.5024 |
| RefSeq: | YP_001118653.1 |
| UniProt: | A4JC15 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARKGIILAG GSGTRLYPIT HAVSKQLLPV YDKPMIYYPL STLMIAGIRD VLIISTPQDT PRFESMLGDG SQWGMNIQYA TQPSPDGLAQ AFVIGRDFVG NEPSALILGD NIFYGHDLAK QLERASAKDT GATVFAYHVQ DPERYGVVEF DREFRAISIE EKPAKPRSSY AVTGLYFYDR QVCDIAADIK PSARGELEIT DVNSRYLAAG QLDVELMGRG YAWLDTGTHD SLIEAGTFIA TLQKRQGLMV ACPEEIAYRR NWIDAEQVLK LAQPLAKNAY GQYLRNLLTH QVAWQST
NT Sequence
ATGGCACGCA AGGGCATCAT TCTCGCCGGC GGTTCAGGCA CACGGCTCTA TCCGATCACG CACGCAGTAT CGAAGCAACT GCTGCCGGTG TACGACAAGC CAATGATCTA CTATCCGCTG TCGACGCTGA TGATTGCAGG CATCCGGGAC GTGCTGATCA TCTCGACGCC GCAGGACACC CCGCGCTTCG AGTCGATGCT CGGCGACGGC AGCCAGTGGG GGATGAATAT CCAGTATGCG ACGCAGCCGT CGCCGGACGG GCTCGCGCAA GCGTTCGTCA TTGGCCGGGA CTTCGTTGGC AACGAGCCGT CGGCGCTCAT CCTCGGCGAC AACATTTTCT ACGGGCACGA TCTCGCGAAG CAGCTCGAGC GAGCCAGCGC GAAGGATACG GGCGCGACCG TATTTGCGTA TCACGTTCAG GACCCCGAGC GGTATGGCGT GGTCGAGTTC GATCGCGAAT TCCGCGCGAT ATCGATTGAA GAGAAGCCGG CAAAGCCGCG TTCGAGCTAT GCGGTCACGG GGCTGTATTT CTACGACAGG CAGGTTTGCG ACATCGCAGC CGACATCAAG CCGTCGGCGC GCGGCGAGCT CGAGATCACC GATGTCAATT CGCGCTACCT GGCGGCGGGC CAGCTCGACG TCGAGCTCAT GGGGCGCGGT TATGCGTGGC TCGATACGGG TACGCACGAT TCCTTGATCG AGGCGGGGAC GTTCATCGCG ACGCTGCAGA AGCGTCAGGG GCTCATGGTT GCGTGTCCCG AAGAGATCGC GTACCGCCGC AACTGGATCG ACGCGGAGCA GGTGCTGAAA CTGGCACAGC CGCTCGCGAA GAACGCATAC GGGCAGTATC TCCGGAACCT ACTCACCCAT CAAGTCGCAT GGCAATCCAC G
Details for BuviA.00118.a.B1.GE39152
| HARVESTED ON: | 5/6/2015 |
| SEQUENCED ON: | 5/6/2015 |
| EXPECTED MW: | 34kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPITHA VSKQLLPVYD KPMIYYPLST LMIAGIRDVL IISTPQDTPR FESMLGDGSQ WGMNIQYATQ PSPDGLAQAF VIGRDFVGNE PSALILGDNI FYGHDLAKQL ERASAKDTGA TVFAYHVQDP ERYGVVEFDR EFRAISIEEK PAKPRSSYAV TGLYFYDRQV CDIAADIKPS ARGELEITDV NSRYLAAGQL DVELMGRGYA WLDTGTHDSL IEAGTFIATL QKRQGLMVAC PEEIAYRRNW IDAEQVLKLA QPLAKNAYGQ YLRNLLTXXX AWQST
Validated NT Sequence
atcttactca cttacgtgga ttgccatgcg annnganngg tgagtaggtt ccggagatac tgcccgtatg cgttcttcgc gagcggctgt gccagtttca gcacctgctc cgcgtcgatc cagttgcggc ggtacgcgat ctcttcggga cacgcaacca tgagcccctg acgcttctgc agcgtcgcga tgaacgtccc cgcctcgatc aaggaatcgt gcgtacccgt atcgagccac gcataaccgc gccccatgag ctcgacgtcg agctggcccg ccgccaggta gcgcgaattg acatcggtga tctcgagctc gccgcgcgcc gacggcttga tgtcggctgc gatgtcgcaa acctgcctgt cgtagaaata cagccccgtg accgcatagc tcgaacgcgg ctttgccggc ttctcttcaa tcgatatcgc gcggaattcg cgatcgaact cgaccacgcc ataccgctcg gggtcctgaa cgtgatacgc aaatacggtc gcgcccgtat ccttcgcgct ggctcgctcg agctgcttcg cgagatcgtg cccgtagaaa atgttgtcgc cgaggatgag cgccgacggc tcgttgccaa cgaagtcccg gccaatgacg aacgcttgcg cgagcccgtc cggcgacggc tgcgtcgcat actggatatt catcccccac tggctgccgt cgccgagcat cgactcgaag cgcggggtgt cctgcggcgt cgagatgatc agcacgtccc ggatgcctgc aatcatcagc gtcgacagcg gatagtagat cattggcttg tcgtacaccg gcagcagttg cttcgatact gcgtgcgtga tcggatagag ccgtgtgcct gaaccgccgg cgagaatgat gcccttgcgt gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPITHA VSKQLLPVYD KPMIYYPLST LMIAGIRDVL IISTPQDTPR FESMLGDGSQ WGMNIQYATQ PSPDGLAQAF VIGRDFVGNE PSALILGDNI FYGHDLAKQL ERASAKDTGA TVFAYHVQDP ERYGVVEFDR EFRAISIEEK PAKPRSSYAV TGLYFYDRQV CDIAADIKPS ARGELEITDV NSRYLAAGQL DVELMGRGYA WLDTGTHDSL IEAGTFIATL QKRQGLMVAC PEEIAYRRNW IDAEQVLKLA QPLAKNAYGQ YLRNLLTHQV AWQST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gcaagggcat cattctcgcc ggcggttcag gcacacggct ctatccgatc acgcacgcag tatcgaagca actgctgccg gtgtacgaca agccaatgat ctactatccg ctgtcgacgc tgatgattgc aggcatccgg gacgtgctga tcatctcgac gccgcaggac accccgcgct tcgagtcgat gctcggcgac ggcagccagt gggggatgaa tatccagtat gcgacgcagc cgtcgccgga cgggctcgcg caagcgttcg tcattggccg ggacttcgtt ggcaacgagc cgtcggcgct catcctcggc gacaacattt tctacgggca cgatctcgcg aagcagctcg agcgagccag cgcgaaggat acgggcgcga ccgtatttgc gtatcacgtt caggaccccg agcggtatgg cgtggtcgag ttcgatcgcg aattccgcgc gatatcgatt gaagagaagc cggcaaagcc gcgttcgagc tatgcggtca cggggctgta tttctacgac aggcaggttt gcgacatcgc agccgacatc aagccgtcgg cgcgcggcga gctcgagatc accgatgtca attcgcgcta cctggcggcg ggccagctcg acgtcgagct catggggcgc ggttatgcgt ggctcgatac gggtacgcac gattccttga tcgaggcggg gacgttcatc gcgacgctgc agaagcgtca ggggctcatg gttgcgtgtc ccgaagagat cgcgtaccgc cgcaactgga tcgacgcgga gcaggtgctg aaactggcac agccgctcgc gaagaacgca tacgggcagt atctccggaa cctactcacc catcaagtcg catggcaatc cacgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat