BuviA.00113.b
3-oxoacyl-[acyl-carrier-protein] synthase II
| CENTER ID: | BuviA.00113.b |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00113.b.A1.GE32431 | Full length( BuviA.00113.b ) | 1 | 430 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00113.b.A1.PS01317 | Full length( BuviA.00113.b ) | 1 | 430 |
Structures
4DDO
DEPOSITED: 1/19/2012
DETERMINATION: XRay
CLONE: BuviA.00113.b.A1.GE32431
PROTEIN: BuviA.00113.b.A1.PS01317
4F32
DEPOSITED: 5/8/2012
DETERMINATION: XRay
CLONE: BuviA.00113.b.A1.GE32431
PROTEIN: BuviA.00113.b.A1.PS01317
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126985 |
| PATRIC ID: | fig|269482.11.peg.2731 |
| RefSeq: | YP_001116448.1 |
| UniProt: | A4JL30 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLRVVVTGI GIVSPLGCGK ELVWQRLIGG GSGLRRLGDD IAGELSAKVG GTVQDVAEDP EGGFDPERSV PHKELRKMDR FIQMAMVAAD EALAEAGWAP EAEQQRERTA TVVASGIGGF PGLAEAVRIG ETRGVRRLSP FTIPFFLSNL AAGQISIKHR FRGPLGCPVT ACAASVQAIG DAMRMIRTGE ADVVLAGGAE AAFDKVSLGG FAAARALSTG FSEEPVRASR PFDRDRDGFV MGEGAAMVVV ESLDHALARG ARPIAEIIGY GTTADAYHMT AGPDDGSGAM RAMKLALRMG DVAPEQVDYV NAHATSTPVG DAGEIEALKT VFGVGAGPAI SSTKSATGHL LGAAGAIEAA FSILALRDGV LPGTLNLEHP DPAADGLDLI GPAARHVPVE IALSNGFGFG GVNASVLFRR YPSDRSAEAH
NT Sequence
ATGACGCTTC GAGTGGTGGT AACCGGTATT GGCATTGTGT CTCCACTGGG TTGTGGGAAG GAATTGGTGT GGCAGCGCCT GATCGGCGGG GGCTCCGGGC TTCGCCGCCT CGGCGACGAT ATCGCCGGTG AGCTGTCCGC CAAGGTCGGC GGGACCGTCC AGGACGTGGC CGAAGATCCG GAAGGCGGCT TCGATCCCGA GCGCTCGGTG CCGCACAAGG AACTGCGGAA GATGGACCGC TTCATCCAGA TGGCGATGGT CGCCGCGGAC GAGGCGCTCG CCGAAGCAGG ATGGGCGCCG GAAGCGGAGC AGCAGCGCGA GCGGACCGCT ACCGTCGTGG CCTCGGGCAT CGGCGGATTT CCCGGCCTGG CGGAGGCGGT GAGAATCGGC GAGACGCGCG GCGTGCGGCG CTTGTCGCCG TTCACCATTC CGTTCTTCCT GTCCAACCTG GCAGCCGGCC AGATTTCGAT CAAGCACCGG TTCCGCGGGC CGCTCGGCTG TCCCGTGACG GCCTGTGCCG CGAGTGTGCA GGCGATCGGC GATGCGATGC GCATGATCAG GACCGGTGAG GCCGATGTCG TCCTCGCCGG CGGCGCAGAA GCGGCATTCG ACAAGGTCAG CCTCGGGGGC TTCGCGGCGG CTCGCGCGCT GTCGACCGGT TTCAGCGAGG AGCCGGTGCG GGCGTCGCGT CCGTTCGATC GCGATCGTGA CGGATTCGTC ATGGGCGAGG GCGCCGCCAT GGTGGTGGTC GAGTCGCTGG ATCATGCCCT GGCGCGCGGC GCCCGGCCGA TCGCGGAAAT CATCGGCTAC GGCACCACGG CCGACGCTTA TCACATGACC GCCGGACCCG ACGACGGATC CGGCGCGATG CGGGCGATGA AGCTGGCCCT GCGGATGGGC GACGTCGCTC CGGAGCAGGT CGACTACGTC AACGCCCATG CGACGTCGAC GCCGGTCGGC GACGCCGGCG AGATCGAGGC GCTGAAGACG GTGTTCGGTG TGGGCGCCGG CCCGGCGATT TCGTCGACCA AGTCGGCAAC CGGACATCTG CTCGGAGCGG CGGGCGCCAT CGAAGCGGCG TTCTCCATTC TGGCTCTGCG CGATGGCGTG CTGCCCGGCA CGCTGAATCT TGAGCACCCC GACCCGGCAG CCGACGGGCT GGATCTGATC GGGCCTGCCG CGCGGCACGT GCCGGTCGAG ATCGCGCTGT CCAACGGGTT CGGGTTCGGA GGCGTGAACG CGTCGGTGCT GTTCAGGCGA TATCCGTCGG ATCGGTCCGC CGAGGCGCAT
Details for BuviA.00113.b.A1.GE32431
| HARVESTED ON: | 11/8/2011 |
| SEQUENCED ON: | 11/11/2011 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTLRVVVTG IGIVSPLGCG KELVWQRLIG GGSGLRRLGD DIAGELSAKV GGTVQDVAED PEGGFDPERS VPHKELRKMD RFIQMAMVAA DEALAEAGWA PEAEQQRERT ATVVASGIGG FPGLAEAVRI GETRGVRRLS PFTIPFFLSN LAAGQISIKH RFRGPLGCPV TACAASVQAI GDAMRMIRTG EADVVLAGGA EAAFDKVSLG GFAAARALST GFSEEPVRAS RPFDRDRDGF VMGEGAAMVV VESLDHALAR GARPIAEIIG YGTTADAYHM TAGPDDGSGA MRAMKLALRM GDVAPEQVDY VNAHATSTPV GDAGEIEALK TVFGVGAGPA ISSTKSATGH LLGAAGAIEA AFSILALRDG VLPGTLNLEH PDPAADGLDL IGPAARHVPV EIALSNGFGF GGVNASVLFR RYPSDRSAEA H
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgc ttcgagtggt ggtaaccggt attggcattg tgtctccact gggttgtggg aaggaattgg tgtggcagcg cctgatcggc gggggctccg ggcttcgccg cctcggcgac gatatcgccg gtgagctgtc cgccaaggtc ggcgggaccg tccaggacgt ggccgaagat ccggaaggcg gcttcgatcc cgagcgctcg gtgccgcaca aggaactgcg gaagatggac cgcttcatcc agatggcgat ggtcgccgcg gacgaggcgc tcgccgaagc aggatgggcg ccggaagcgg agcagcagcg cgagcggacc gctaccgtcg tggcctcggg catcggcgga tttcccggcc tggcggaggc ggtgagaatc ggcgagacgc gcggcgtgcg gcgcttgtcg ccgttcacca ttccgttctt cctgtccaac ctggcagccg gccagatttc gatcaagcac cggttccgcg ggccgctcgg ctgtcccgtg acggcctgtg ccgcgagtgt gcaggcgatc ggcgatgcga tgcgcatgat caggaccggt gaggccgatg tcgtcctcgc cggcggcgca gaagcggcat tcgacaaggt cagcctcggg ggcttcgcgg cggctcgcgc gctgtcgacc ggtttcagcg aggagccggt gcgggcgtcg cgtccgttcg atcgcgatcg tgacggattc gtcatgggcg agggcgccgc catggtggtg gtcgagtcgc tggatcatgc cctggcgcgc ggcgcccggc cgatcgcgga aatcatcggc tacggcacca cggccgacgc ttatcacatg accgccggac ccgacgacgg atccggcgcg atgcgggcga tgaagctggc cctgcggatg ggcgacgtcg ctccggagca ggtcgactac gtcaacgccc atgcgacgtc gacgccggtc ggcgacgccg gcgagatcga ggcgctgaag acggtgttcg gtgtgggcgc cggcccggcg atttcgtcga ccaagtcggc aaccggacat ctgctcggag cggcgggcgc catcgaagcg gcgttctcca ttctggctct gcgcgatggc gtgctgcccg gcacgctgaa tcttgagcac cccgacccgg cagccgacgg gctggatctg atcgggcctg ccgcgcggca cgtgccggtc gagatcgcgc tgtccaacgg gttcgggttc ggaggcgtga acgcgtcggt gctgttcagg cgatatccgt cggatcggtc cgccgaggcg cattaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLRVVVTG IGIVSPLGCG KELVWQRLIG GGSGLRRLGD DIAGELSAKV GGTVQDVAED PEGGFDPERS VPHKELRKMD RFIQMAMVAA DEALAEAGWA PEAEQQRERT ATVVASGIGG FPGLAEAVRI GETRGVRRLS PFTIPFFLSN LAAGQISIKH RFRGPLGCPV TACAASVQAI GDAMRMIRTG EADVVLAGGA EAAFDKVSLG GFAAARALST GFSEEPVRAS RPFDRDRDGF VMGEGAAMVV VESLDHALAR GARPIAEIIG YGTTADAYHM TAGPDDGSGA MRAMKLALRM GDVAPEQVDY VNAHATSTPV GDAGEIEALK TVFGVGAGPA ISSTKSATGH LLGAAGAIEA AFSILALRDG VLPGTLNLEH PDPAADGLDL IGPAARHVPV EIALSNGFGF GGVNASVLFR RYPSDRSAEA H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcttcga gtggtggtaa ccggtattgg cattgtgtct ccactgggtt gtgggaagga attggtgtgg cagcgcctga tcggcggggg ctccgggctt cgccgcctcg gcgacgatat cgccggtgag ctgtccgcca aggtcggcgg gaccgtccag gacgtggccg aagatccgga aggcggcttc gatcccgagc gctcggtgcc gcacaaggaa ctgcggaaga tggaccgctt catccagatg gcgatggtcg ccgcggacga ggcgctcgcc gaagcaggat gggcgccgga agcggagcag cagcgcgagc ggaccgctac cgtcgtggcc tcgggcatcg gcggatttcc cggcctggcg gaggcggtga gaatcggcga gacgcgcggc gtgcggcgct tgtcgccgtt caccattccg ttcttcctgt ccaacctggc agccggccag atttcgatca agcaccggtt ccgcgggccg ctcggctgtc ccgtgacggc ctgtgccgcg agtgtgcagg cgatcggcga tgcgatgcgc atgatcagga ccggtgaggc cgatgtcgtc ctcgccggcg gcgcagaagc ggcattcgac aaggtcagcc tcgggggctt cgcggcggct cgcgcgctgt cgaccggttt cagcgaggag ccggtgcggg cgtcgcgtcc gttcgatcgc gatcgtgacg gattcgtcat gggcgagggc gccgccatgg tggtggtcga gtcgctggat catgccctgg cgcgcggcgc ccggccgatc gcggaaatca tcggctacgg caccacggcc gacgcttatc acatgaccgc cggacccgac gacggatccg gcgcgatgcg ggcgatgaag ctggccctgc ggatgggcga cgtcgctccg gagcaggtcg actacgtcaa cgcccatgcg acgtcgacgc cggtcggcga cgccggcgag atcgaggcgc tgaagacggt gttcggtgtg ggcgccggcc cggcgatttc gtcgaccaag tcggcaaccg gacatctgct cggagcggcg ggcgccatcg aagcggcgtt ctccattctg gctctgcgcg atggcgtgct gcccggcacg ctgaatcttg agcaccccga cccggcagcc gacgggctgg atctgatcgg gcctgccgcg cggcacgtgc cggtcgagat cgcgctgtcc aacgggttcg ggttcggagg cgtgaacgcg tcggtgctgt tcaggcgata tccgtcggat cggtccgccg aggcgcatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuviA.00113.b.A1.PS01317
| PURIFICATION DATe: | 12/28/2011 |
| CONCENTRATION: | 43mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTLRVVVTG IGIVSPLGCG KELVWQRLIG GGSGLRRLGD DIAGELSAKV GGTVQDVAED PEGGFDPERS VPHKELRKMD RFIQMAMVAA DEALAEAGWA PEAEQQRERT ATVVASGIGG FPGLAEAVRI GETRGVRRLS PFTIPFFLSN LAAGQISIKH RFRGPLGCPV TACAASVQAI GDAMRMIRTG EADVVLAGGA EAAFDKVSLG GFAAARALST GFSEEPVRAS RPFDRDRDGF VMGEGAAMVV VESLDHALAR GARPIAEIIG YGTTADAYHM TAGPDDGSGA MRAMKLALRM GDVAPEQVDY VNAHATSTPV GDAGEIEALK TVFGVGAGPA ISSTKSATGH LLGAAGAIEA AFSILALRDG VLPGTLNLEH PDPAADGLDL IGPAARHVPV EIALSNGFGF GGVNASVLFR RYPSDRSAEA H
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacgc ttcgagtggt ggtaaccggt attggcattg tgtctccact gggttgtggg aaggaattgg tgtggcagcg cctgatcggc gggggctccg ggcttcgccg cctcggcgac gatatcgccg gtgagctgtc cgccaaggtc ggcgggaccg tccaggacgt ggccgaagat ccggaaggcg gcttcgatcc cgagcgctcg gtgccgcaca aggaactgcg gaagatggac cgcttcatcc agatggcgat ggtcgccgcg gacgaggcgc tcgccgaagc aggatgggcg ccggaagcgg agcagcagcg cgagcggacc gctaccgtcg tggcctcggg catcggcgga tttcccggcc tggcggaggc ggtgagaatc ggcgagacgc gcggcgtgcg gcgcttgtcg ccgttcacca ttccgttctt cctgtccaac ctggcagccg gccagatttc gatcaagcac cggttccgcg ggccgctcgg ctgtcccgtg acggcctgtg ccgcgagtgt gcaggcgatc ggcgatgcga tgcgcatgat caggaccggt gaggccgatg tcgtcctcgc cggcggcgca gaagcggcat tcgacaaggt cagcctcggg ggcttcgcgg cggctcgcgc gctgtcgacc ggtttcagcg aggagccggt gcgggcgtcg cgtccgttcg atcgcgatcg tgacggattc gtcatgggcg agggcgccgc catggtggtg gtcgagtcgc tggatcatgc cctggcgcgc ggcgcccggc cgatcgcgga aatcatcggc tacggcacca cggccgacgc ttatcacatg accgccggac ccgacgacgg atccggcgcg atgcgggcga tgaagctggc cctgcggatg ggcgacgtcg ctccggagca ggtcgactac gtcaacgccc atgcgacgtc gacgccggtc ggcgacgccg gcgagatcga ggcgctgaag acggtgttcg gtgtgggcgc cggcccggcg atttcgtcga ccaagtcggc aaccggacat ctgctcggag cggcgggcgc catcgaagcg gcgttctcca ttctggctct gcgcgatggc gtgctgcccg gcacgctgaa tcttgagcac cccgacccgg cagccgacgg gctggatctg atcgggcctg ccgcgcggca cgtgccggtc gagatcgcgc tgtccaacgg gttcgggttc ggaggcgtga acgcgtcggt gctgttcagg cgatatccgt cggatcggtc cgccgaggcg cattaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLRVVVTG IGIVSPLGCG KELVWQRLIG GGSGLRRLGD DIAGELSAKV GGTVQDVAED PEGGFDPERS VPHKELRKMD RFIQMAMVAA DEALAEAGWA PEAEQQRERT ATVVASGIGG FPGLAEAVRI GETRGVRRLS PFTIPFFLSN LAAGQISIKH RFRGPLGCPV TACAASVQAI GDAMRMIRTG EADVVLAGGA EAAFDKVSLG GFAAARALST GFSEEPVRAS RPFDRDRDGF VMGEGAAMVV VESLDHALAR GARPIAEIIG YGTTADAYHM TAGPDDGSGA MRAMKLALRM GDVAPEQVDY VNAHATSTPV GDAGEIEALK TVFGVGAGPA ISSTKSATGH LLGAAGAIEA AFSILALRDG VLPGTLNLEH PDPAADGLDL IGPAARHVPV EIALSNGFGF GGVNASVLFR RYPSDRSAEA H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCTTCGA GTGGTGGTAA CCGGTATTGG CATTGTGTCT CCACTGGGTT GTGGGAAGGA ATTGGTGTGG CAGCGCCTGA TCGGCGGGGG CTCCGGGCTT CGCCGCCTCG GCGACGATAT CGCCGGTGAG CTGTCCGCCA AGGTCGGCGG GACCGTCCAG GACGTGGCCG AAGATCCGGA AGGCGGCTTC GATCCCGAGC GCTCGGTGCC GCACAAGGAA CTGCGGAAGA TGGACCGCTT CATCCAGATG GCGATGGTCG CCGCGGACGA GGCGCTCGCC GAAGCAGGAT GGGCGCCGGA AGCGGAGCAG CAGCGCGAGC GGACCGCTAC CGTCGTGGCC TCGGGCATCG GCGGATTTCC CGGCCTGGCG GAGGCGGTGA GAATCGGCGA GACGCGCGGC GTGCGGCGCT TGTCGCCGTT CACCATTCCG TTCTTCCTGT CCAACCTGGC AGCCGGCCAG ATTTCGATCA AGCACCGGTT CCGCGGGCCG CTCGGCTGTC CCGTGACGGC CTGTGCCGCG AGTGTGCAGG CGATCGGCGA TGCGATGCGC ATGATCAGGA CCGGTGAGGC CGATGTCGTC CTCGCCGGCG GCGCAGAAGC GGCATTCGAC AAGGTCAGCC TCGGGGGCTT CGCGGCGGCT CGCGCGCTGT CGACCGGTTT CAGCGAGGAG CCGGTGCGGG CGTCGCGTCC GTTCGATCGC GATCGTGACG GATTCGTCAT GGGCGAGGGC GCCGCCATGG TGGTGGTCGA GTCGCTGGAT CATGCCCTGG CGCGCGGCGC CCGGCCGATC GCGGAAATCA TCGGCTACGG CACCACGGCC GACGCTTATC ACATGACCGC CGGACCCGAC GACGGATCCG GCGCGATGCG GGCGATGAAG CTGGCCCTGC GGATGGGCGA CGTCGCTCCG GAGCAGGTCG ACTACGTCAA CGCCCATGCG ACGTCGACGC CGGTCGGCGA CGCCGGCGAG ATCGAGGCGC TGAAGACGGT GTTCGGTGTG GGCGCCGGCC CGGCGATTTC GTCGACCAAG TCGGCAACCG GACATCTGCT CGGAGCGGCG GGCGCCATCG AAGCGGCGTT CTCCATTCTG GCTCTGCGCG ATGGCGTGCT GCCCGGCACG CTGAATCTTG AGCACCCCGA CCCGGCAGCC GACGGGCTGG ATCTGATCGG GCCTGCCGCG CGGCACGTGC CGGTCGAGAT CGCGCTGTCC AACGGGTTCG GGTTCGGAGG CGTGAACGCG TCGGTGCTGT TCAGGCGATA TCCGTCGGAT CGGTCCGCCG AGGCGCATTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A