BuviA.00107.e

Efflux transporter, RND family, MFP subunit

CENTER ID: BuviA.00107.e
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.e.A2.GE32429 Mature protein( BuviA.00107.e ) 29 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4153
RefSeq: YP_001117813.1
UniProt: A4JPZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRSVFLAAG ALVVLSGALF LWRTERVSAA DRPASPPAPL PVETIVVKPQ ADAPTLEAVG SLEAVRQVTI APEVAGRVVD IRFVAGSRVS AGQTLVQLYD APEQAKLAGL QAKADYTRRQ FDRAVQLVPN GAEPKATFDA RRYELDAARA DIRETRAVIT QKLIRAPFSG VIGLRRVNLG QYLNPGDPIA TLTDLGVLYA NFTVPQKELS RVSLGQTVRV TTDAYPGRTF EGHVTAIEPQ VGDETRNITV QATVTNTGQL LRPGMYIDAH LALPGRDAVI SVPDTAVQTS QAGDTVVVVE QRDGRGVGVA RVRQVRAGAR ERGRIVIEDG LRAGDVVVTT GQLRVAPGAK VQALADAGTR RETVR
NT Sequence
GTGAGACGCT CTGTGTTCCT TGCCGCAGGC GCGTTGGTCG TCCTGTCCGG CGCATTGTTC CTTTGGCGCA CGGAACGTGT ATCGGCGGCC GATCGGCCTG CGTCGCCACC GGCGCCGTTG CCGGTCGAAA CCATCGTCGT GAAGCCGCAG GCCGATGCGC CGACGCTCGA GGCGGTCGGC TCGCTCGAAG CGGTGCGGCA GGTGACCATC GCGCCGGAAG TCGCCGGCCG CGTCGTCGAC ATCCGGTTCG TCGCGGGCTC GCGCGTGAGC GCCGGGCAAA CGCTCGTGCA ACTGTACGAC GCACCCGAGC AGGCGAAGCT CGCCGGCTTG CAGGCGAAGG CCGACTACAC GCGCCGGCAG TTCGACCGCG CCGTGCAACT CGTGCCGAAC GGCGCCGAGC CGAAGGCGAC ATTCGACGCA CGCCGCTACG AGCTCGACGC CGCGCGCGCC GATATCCGCG AGACTCGAGC GGTCATCACG CAAAAGCTGA TACGCGCGCC GTTTTCCGGC GTGATCGGCT TGCGCCGCGT GAACCTGGGG CAGTACCTGA ACCCGGGCGA TCCGATCGCG ACGCTGACGG ATCTCGGCGT GTTGTACGCG AACTTCACGG TGCCGCAGAA GGAGTTGAGC CGCGTGTCGC TCGGCCAGAC GGTGCGTGTG ACGACGGATG CGTATCCGGG CCGGACCTTC GAAGGCCACG TGACGGCGAT CGAGCCGCAG GTCGGCGACG AGACCCGCAA CATCACCGTG CAGGCCACGG TGACCAATAC CGGCCAGTTG CTGCGCCCCG GCATGTATAT CGACGCGCAT CTGGCGCTGC CCGGCCGCGA CGCGGTGATC AGCGTGCCGG ATACCGCCGT GCAGACCTCC CAGGCGGGCG ACACCGTGGT GGTGGTCGAG CAGCGCGACG GGCGCGGCGT CGGCGTTGCG CGCGTGCGCC AGGTGCGTGC CGGCGCGCGC GAGCGTGGCC GCATCGTCAT CGAGGACGGG CTGCGCGCCG GCGACGTGGT CGTGACGACC GGGCAGTTGC GCGTCGCACC GGGCGCGAAG GTGCAGGCGC TCGCCGATGC GGGCACACGA CGGGAGACGG TGCGA
Details for BuviA.00107.e.A2.GE32429
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 38kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAADRPASPP APLPVETIVV KPQADAPTLE AVGSLEAVRQ VTIAPEVAGR VVDIRFVAGS RVSAGQTLVQ LYDAPEQAKL AGLQAKADYT RRQFDRAVQL VPNGAEPKAT FDARRYELDA ARADIRETRA VITQKLIRAP FSGVIGLRRV NLGQYLNPGD PIATLTDLGV LYANFTVPQK ELSRVSLGQT VRVTTDAYPG RTFEGHVTAI EPQVGDETRN ITVQATVTNT GQLLRPGMYI DAHLALPGRD AVISVPDTAV QTSQAGDTVV VVEQRDGRGV GVARVRQVRA GARERGRIVI EDGLRAGDVV VTTGQLRVAP GAKVQALADA GTRRETVR
Validated NT Sequence
ttattatcgc accgtctccc gtcgtgtgcc cgcatcggcg agcgcctgca ccttcgcgcc cggtgcgacg cgcaactgcc cggtcgtcac gaccacgtcg ccggcgcgca gcccgtcctc gatgacgatg cggccacgct cgcgcgcgcc ggcacgcacc tggcgcacgc gcgcaacgcc gacgccgcgc ccgtcgcgct gctcgaccac caccacggtg tcgcccgcct gggaggtctg cacggcggta tccggcacgc tgatcaccgc gtcgcggccg ggcagcgcca gatgcgcgtc gatatacatg ccggggcgca gcaactggcc ggtattggtc accgtggcct gcacggtgat gttgcgggtc tcgtcgccga cctgcggctc gatcgccgtc acgtggcctt cgaaggtccg gcccggatac gcatccgtcg tcacacgcac cgtctggccg agcgacacgc ggctcaactc cttctgcggc accgtgaagt tcgcgtacaa cacgccgaga tccgtcagcg tcgcgatcgg atcgcccggg ttcaggtact gccccaggtt cacgcggcgc aagccgatca cgccggaaaa cggcgcgcgt atcagctttt gcgtgatgac cgctcgagtc tcgcggatat cggcgcgcgc ggcgtcgagc tcgtagcggc gtgcgtcgaa tgtcgccttc ggctcggcgc cgttcggcac gagttgcacg gcgcggtcga actgccggcg cgtgtagtcg gccttcgcct gcaagccggc gagcttcgcc tgctcgggtg cgtcgtacag ttgcacgagc gtttgcccgg cgctcacgcg cgagcccgcg acgaaccgga tgtcgacgac gcggccggcg acttccggcg cgatggtcac ctgccgcacc gcttcgagcg agccgaccgc ctcgagcgtc ggcgcatcgg cctgcggctt cacgacgatg gtttcgaccg gcaacggcgc cggtggcgac gcaggccgat cggccgccat cgaaccagga ccctgggtct gagcttccag ggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAADRPASP PAPLPVETIV VKPQADAPTL EAVGSLEAVR QVTIAPEVAG RVVDIRFVAG SRVSAGQTLV QLYDAPEQAK LAGLQAKADY TRRQFDRAVQ LVPNGAEPKA TFDARRYELD AARADIRETR AVITQKLIRA PFSGVIGLRR VNLGQYLNPG DPIATLTDLG VLYANFTVPQ KELSRVSLGQ TVRVTTDAYP GRTFEGHVTA IEPQVGDETR NITVQATVTN TGQLLRPGMY IDAHLALPGR DAVISVPDTA VQTSQAGDTV VVVEQRDGRG VGVARVRQVR AGARERGRIV IEDGLRAGDV VVTTGQLRVA PGAKVQALAD AGTRRETVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccgat cggcctgcgt cgccaccggc gccgttgccg gtcgaaacca tcgtcgtgaa gccgcaggcc gatgcgccga cgctcgaggc ggtcggctcg ctcgaagcgg tgcggcaggt gaccatcgcg ccggaagtcg ccggccgcgt cgtcgacatc cggttcgtcg cgggctcgcg cgtgagcgcc gggcaaacgc tcgtgcaact gtacgacgca cccgagcagg cgaagctcgc cggcttgcag gcgaaggccg actacacgcg ccggcagttc gaccgcgccg tgcaactcgt gccgaacggc gccgagccga aggcgacatt cgacgcacgc cgctacgagc tcgacgccgc gcgcgccgat atccgcgaga ctcgagcggt catcacgcaa aagctgatac gcgcgccgtt ttccggcgtg atcggcttgc gccgcgtgaa cctggggcag tacctgaacc cgggcgatcc gatcgcgacg ctgacggatc tcggcgtgtt gtacgcgaac ttcacggtgc cgcagaagga gttgagccgc gtgtcgctcg gccagacggt gcgtgtgacg acggatgcgt atccgggccg gaccttcgaa ggccacgtga cggcgatcga gccgcaggtc ggcgacgaga cccgcaacat caccgtgcag gccacggtga ccaataccgg ccagttgctg cgccccggca tgtatatcga cgcgcatctg gcgctgcccg gccgcgacgc ggtgatcagc gtgccggata ccgccgtgca gacctcccag gcgggcgaca ccgtggtggt ggtcgagcag cgcgacgggc gcggcgtcgg cgttgcgcgc gtgcgccagg tgcgtgccgg cgcgcgcgag cgtggccgca tcgtcatcga ggacgggctg cgcgccggcg acgtggtcgt gacgaccggg cagttgcgcg tcgcaccggg cgcgaaggtg caggcgctcg ccgatgcggg cacacgacgg gagacggtgc gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa