BuviA.00107.d

Efflux transporter, RND family, MFP subunit

CENTER ID: BuviA.00107.d
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.d.A2.GE34870 Mature protein( BuviA.00107.d ) 49 427
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.d.A2.PW35485 Mature protein( BuviA.00107.d ) 49 427

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3484
RefSeq: YP_001117182.1
UniProt: A4JN64

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTPILPNHA HRRMTCRPRQ LFAALSILAI VGTAGCNRQA GGPPGGAAAQ TAQVGVLTLA PQRIVETTEL SGRLSSQKVS DVRPQVSGII LKRLFVEGGD VKAGQVLYQI DPATYRAAYD QARGTLENAR ATLADAKTKA DRYTELVKIN AVSKQDYDDA VAAVHQDAAT VTADAAALES ARVNLEYTRV RAPISGRIGA STVTEGALVT SAQTTALATI QAFDQMYLDV TRPSTEWLKL RKAFASGRLK KTGSDSAAVT LVMEDGTEYA HAGTLQFSGV TVDATTGSVT LRSTFPNPER ELLPGMFVRA RLEEGENDDA ILVPQMAVTR ASDGKASVYV VGAAGNAQQV QVTADNAFRD QWIVTSGLHA GDRVIVSGLQ SVHAGAPVKV AADTPQTQGN AVSAASAASA ATDTSAAGTA SPVSAQH
NT Sequence
ATGAAAACGC CAATCCTCCC CAACCACGCG CACCGCCGCA TGACGTGCAG GCCGCGCCAG CTGTTTGCCG CCCTCTCGAT ACTGGCGATA GTCGGCACCG CCGGCTGCAA CAGGCAAGCA GGCGGGCCGC CCGGCGGCGC CGCCGCGCAG ACCGCGCAGG TGGGCGTGCT CACGCTCGCG CCGCAGCGCA TCGTCGAAAC CACCGAACTC TCTGGCCGCC TTTCCTCGCA GAAGGTCTCC GACGTCCGAC CGCAGGTGAG CGGCATCATC CTCAAGCGCC TGTTCGTGGA AGGCGGTGAC GTAAAGGCCG GCCAGGTGCT CTACCAGATC GATCCGGCCA CCTACCGGGC GGCCTACGAC CAGGCGCGCG GCACGCTCGA AAACGCCCGC GCCACGTTGG CCGATGCGAA GACGAAAGCG GACCGCTACA CGGAACTCGT GAAGATCAAC GCCGTGAGCA AGCAGGACTA CGACGACGCC GTCGCCGCCG TCCACCAGGA CGCCGCCACC GTGACGGCCG ACGCGGCCGC GCTCGAATCG GCGCGCGTGA ACCTCGAATA CACGCGCGTG CGGGCGCCCA TTTCAGGACG CATCGGCGCC TCCACCGTGA CCGAAGGCGC GCTCGTCACG TCCGCCCAGA CCACTGCGCT CGCCACCATC CAGGCGTTCG ATCAGATGTA TCTCGACGTC ACGCGTCCCA GCACGGAGTG GCTGAAGCTG CGCAAGGCCT TCGCTTCGGG GCGCCTCAAG AAGACGGGCA GCGACAGCGC CGCCGTCACG CTCGTGATGG AGGACGGCAC CGAATACGCC CACGCCGGCA CGCTGCAGTT CTCCGGCGTG ACGGTCGATG CCACGACGGG CTCGGTCACG CTGCGCAGTA CGTTCCCGAA CCCCGAACGC GAATTGCTGC CCGGCATGTT CGTGCGCGCC CGCCTGGAAG AAGGCGAGAA CGACGACGCG ATACTCGTGC CGCAGATGGC CGTGACGCGC GCGTCGGACG GCAAGGCGAG CGTCTACGTG GTGGGTGCGG CCGGCAATGC GCAGCAGGTG CAGGTGACGG CCGACAACGC ATTCCGCGAC CAGTGGATCG TGACCTCGGG CCTGCACGCC GGCGACCGCG TGATCGTGAG CGGCCTGCAG AGCGTGCATG CGGGCGCGCC GGTCAAGGTG GCCGCCGATA CGCCGCAGAC GCAGGGGAAC GCGGTATCTG CGGCATCTGC GGCGTCCGCT GCCACCGACA CATCCGCAGC CGGCACGGCC TCGCCCGTGT CGGCGCAACA C
Details for BuviA.00107.d.A2.GE34870
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 42kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTAQVGV LTLAPQRIVE TTELSGRLSS QKVSDVRPQV SGIILKRLFV EGGDVKAGQV LYQIDPATYR AAYDQARGTL ENARATLADA KTKADRYTEL VKINAVSKQD YDDAVAAVHQ DAATVTADAA ALESARVNLE YTRVRAPISG RIGASTVTEG ALVTSAQTTA LATIQAFDQM YLDVTRPSTE WLKLRKAFAS GRLKKTGSDS AAVTLVMEDG TEYAHAGTLQ FSGVTVDATT GSVTLRSTFP NPERELLPGM FVRARLEEGE NDDAILVPQM AVTRASDGKA SVYVVGAAGN AQQVQVTADN AFRDQWIVTS GLHAGDRVIV SGLQSVHAGA PVKVAADTPQ TQGNAVSAAS AASAATDTSA AGTASPVS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgc agaccgctca ggtgggcgtg ctcacgctcg cgccgcagcg catcgtcgaa accaccgaac tctctggccg cctttcctcg cagaaggtct ccgacgtccg accgcaggtg agcggcatca tcctcaagcg cctgttcgtg gaaggcggtg acgtaaaggc cggccaggtg ctctaccaga tcgatccggc cacctaccgg gcggcctacg accaggcgcg cggcacgctc gaaaacgccc gcgccacgtt ggccgatgcg aagacgaaag cggaccgcta cacggaactc gtgaagatca acgccgtgag caagcaggac tacgacgacg ccgtcgccgc cgtccaccag gacgccgcca ccgtgacggc cgacgcggcc gcgctcgaat cggcgcgcgt gaacctcgaa tacacgcgcg tgcgggcgcc catttcagga cgcatcggcg cctccaccgt gaccgaaggc gcgctcgtca cgtccgccca gaccactgcg ctcgccacca tccaggcgtt cgatcagatg tatctcgacg tcacgcgtcc cagcacggag tggctgaagc tgcgcaaggc cttcgcttcg gggcgcctca agaagacggg cagcgacagc gccgccgtca cgctcgtgat ggaggacggc accgaatacg cccacgccgg cacgctgcag ttctccggcg tgacggtcga tgccacgacg ggctcggtca cgctgcgcag tacgttcccg aaccccgaac gcgaattgct gcccggcatg ttcgtgcgcg cccgcctgga agaaggcgag aacgacgacg cgatactcgt gccgcagatg gccgtgacgc gcgcgtcgga cggcaaggcg agcgtctacg tggtgggtgc ggccggcaat gcgcagcagg tgcaggtgac ggccgacaac gcattccgcg accagtggat cgtgacctcg ggcctgcacg ccggcgaccg cgtgatcgtg agcggcctgc agagcgtgca tgcgggcgcg ccggtcaagg tggccgccga tacgccgcag acgcagggga acgcggtatc tgcggcatct gcggcgtccg ctgccaccga cacatccgca gccggcacgg cctcgcccgt gtcggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTAQVGV LTLAPQRIVE TTELSGRLSS QKVSDVRPQV SGIILKRLFV EGGDVKAGQV LYQIDPATYR AAYDQARGTL ENARATLADA KTKADRYTEL VKINAVSKQD YDDAVAAVHQ DAATVTADAA ALESARVNLE YTRVRAPISG RIGASTVTEG ALVTSAQTTA LATIQAFDQM YLDVTRPSTE WLKLRKAFAS GRLKKTGSDS AAVTLVMEDG TEYAHAGTLQ FSGVTVDATT GSVTLRSTFP NPERELLPGM FVRARLEEGE NDDAILVPQM AVTRASDGKA SVYVVGAAGN AQQVQVTADN AFRDQWIVTS GLHAGDRVIV SGLQSVHAGA PVKVAADTPQ TQGNAVSAAS AASAATDTSA AGTASPVSAQ H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcagacc gcgcaggtgg gcgtgctcac gctcgcgccg cagcgcatcg tcgaaaccac cgaactctct ggccgccttt cctcgcagaa ggtctccgac gtccgaccgc aggtgagcgg catcatcctc aagcgcctgt tcgtggaagg cggtgacgta aaggccggcc aggtgctcta ccagatcgat ccggccacct accgggcggc ctacgaccag gcgcgcggca cgctcgaaaa cgcccgcgcc acgttggccg atgcgaagac gaaagcggac cgctacacgg aactcgtgaa gatcaacgcc gtgagcaagc aggactacga cgacgccgtc gccgccgtcc accaggacgc cgccaccgtg acggccgacg cggccgcgct cgaatcggcg cgcgtgaacc tcgaatacac gcgcgtgcgg gcgcccattt caggacgcat cggcgcctcc accgtgaccg aaggcgcgct cgtcacgtcc gcccagacca ctgcgctcgc caccatccag gcgttcgatc agatgtatct cgacgtcacg cgtcccagca cggagtggct gaagctgcgc aaggccttcg cttcggggcg cctcaagaag acgggcagcg acagcgccgc cgtcacgctc gtgatggagg acggcaccga atacgcccac gccggcacgc tgcagttctc cggcgtgacg gtcgatgcca cgacgggctc ggtcacgctg cgcagtacgt tcccgaaccc cgaacgcgaa ttgctgcccg gcatgttcgt gcgcgcccgc ctggaagaag gcgagaacga cgacgcgata ctcgtgccgc agatggccgt gacgcgcgcg tcggacggca aggcgagcgt ctacgtggtg ggtgcggccg gcaatgcgca gcaggtgcag gtgacggccg acaacgcatt ccgcgaccag tggatcgtga cctcgggcct gcacgccggc gaccgcgtga tcgtgagcgg cctgcagagc gtgcatgcgg gcgcgccggt caaggtggcc gccgatacgc cgcagacgca ggggaacgcg gtatctgcgg catctgcggc gtccgctgcc accgacacat ccgcagccgg cacggcctcg cccgtgtcgg cgcaacacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuviA.00107.d.A2.PW35485
PURIFICATION DATe: 6/15/2012
CONCENTRATION: 29.03mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTAQVGV LTLAPQRIVE TTELSGRLSS QKVSDVRPQV SGIILKRLFV EGGDVKAGQV LYQIDPATYR AAYDQARGTL ENARATLADA KTKADRYTEL VKINAVSKQD YDDAVAAVHQ DAATVTADAA ALESARVNLE YTRVRAPISG RIGASTVTEG ALVTSAQTTA LATIQAFDQM YLDVTRPSTE WLKLRKAFAS GRLKKTGSDS AAVTLVMEDG TEYAHAGTLQ FSGVTVDATT GSVTLRSTFP NPERELLPGM FVRARLEEGE NDDAILVPQM AVTRASDGKA SVYVVGAAGN AQQVQVTADN AFRDQWIVTS GLHAGDRVIV SGLQSVHAGA PVKVAADTPQ TQGNAVSAAS AASAATDTSA AGTASPVS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgc agaccgctca ggtgggcgtg ctcacgctcg cgccgcagcg catcgtcgaa accaccgaac tctctggccg cctttcctcg cagaaggtct ccgacgtccg accgcaggtg agcggcatca tcctcaagcg cctgttcgtg gaaggcggtg acgtaaaggc cggccaggtg ctctaccaga tcgatccggc cacctaccgg gcggcctacg accaggcgcg cggcacgctc gaaaacgccc gcgccacgtt ggccgatgcg aagacgaaag cggaccgcta cacggaactc gtgaagatca acgccgtgag caagcaggac tacgacgacg ccgtcgccgc cgtccaccag gacgccgcca ccgtgacggc cgacgcggcc gcgctcgaat cggcgcgcgt gaacctcgaa tacacgcgcg tgcgggcgcc catttcagga cgcatcggcg cctccaccgt gaccgaaggc gcgctcgtca cgtccgccca gaccactgcg ctcgccacca tccaggcgtt cgatcagatg tatctcgacg tcacgcgtcc cagcacggag tggctgaagc tgcgcaaggc cttcgcttcg gggcgcctca agaagacggg cagcgacagc gccgccgtca cgctcgtgat ggaggacggc accgaatacg cccacgccgg cacgctgcag ttctccggcg tgacggtcga tgccacgacg ggctcggtca cgctgcgcag tacgttcccg aaccccgaac gcgaattgct gcccggcatg ttcgtgcgcg cccgcctgga agaaggcgag aacgacgacg cgatactcgt gccgcagatg gccgtgacgc gcgcgtcgga cggcaaggcg agcgtctacg tggtgggtgc ggccggcaat gcgcagcagg tgcaggtgac ggccgacaac gcattccgcg accagtggat cgtgacctcg ggcctgcacg ccggcgaccg cgtgatcgtg agcggcctgc agagcgtgca tgcgggcgcg ccggtcaagg tggccgccga tacgccgcag acgcagggga acgcggtatc tgcggcatct gcggcgtccg ctgccaccga cacatccgca gccggcacgg cctcgcccgt gtcggcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQTAQVGV LTLAPQRIVE TTELSGRLSS QKVSDVRPQV SGIILKRLFV EGGDVKAGQV LYQIDPATYR AAYDQARGTL ENARATLADA KTKADRYTEL VKINAVSKQD YDDAVAAVHQ DAATVTADAA ALESARVNLE YTRVRAPISG RIGASTVTEG ALVTSAQTTA LATIQAFDQM YLDVTRPSTE WLKLRKAFAS GRLKKTGSDS AAVTLVMEDG TEYAHAGTLQ FSGVTVDATT GSVTLRSTFP NPERELLPGM FVRARLEEGE NDDAILVPQM AVTRASDGKA SVYVVGAAGN AQQVQVTADN AFRDQWIVTS GLHAGDRVIV SGLQSVHAGA PVKVAADTPQ TQGNAVSAAS AASAATDTSA AGTASPVSAQ H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCAGACC GCGCAGGTGG GCGTGCTCAC GCTCGCGCCG CAGCGCATCG TCGAAACCAC CGAACTCTCT GGCCGCCTTT CCTCGCAGAA GGTCTCCGAC GTCCGACCGC AGGTGAGCGG CATCATCCTC AAGCGCCTGT TCGTGGAAGG CGGTGACGTA AAGGCCGGCC AGGTGCTCTA CCAGATCGAT CCGGCCACCT ACCGGGCGGC CTACGACCAG GCGCGCGGCA CGCTCGAAAA CGCCCGCGCC ACGTTGGCCG ATGCGAAGAC GAAAGCGGAC CGCTACACGG AACTCGTGAA GATCAACGCC GTGAGCAAGC AGGACTACGA CGACGCCGTC GCCGCCGTCC ACCAGGACGC CGCCACCGTG ACGGCCGACG CGGCCGCGCT CGAATCGGCG CGCGTGAACC TCGAATACAC GCGCGTGCGG GCGCCCATTT CAGGACGCAT CGGCGCCTCC ACCGTGACCG AAGGCGCGCT CGTCACGTCC GCCCAGACCA CTGCGCTCGC CACCATCCAG GCGTTCGATC AGATGTATCT CGACGTCACG CGTCCCAGCA CGGAGTGGCT GAAGCTGCGC AAGGCCTTCG CTTCGGGGCG CCTCAAGAAG ACGGGCAGCG ACAGCGCCGC CGTCACGCTC GTGATGGAGG ACGGCACCGA ATACGCCCAC GCCGGCACGC TGCAGTTCTC CGGCGTGACG GTCGATGCCA CGACGGGCTC GGTCACGCTG CGCAGTACGT TCCCGAACCC CGAACGCGAA TTGCTGCCCG GCATGTTCGT GCGCGCCCGC CTGGAAGAAG GCGAGAACGA CGACGCGATA CTCGTGCCGC AGATGGCCGT GACGCGCGCG TCGGACGGCA AGGCGAGCGT CTACGTGGTG GGTGCGGCCG GCAATGCGCA GCAGGTGCAG GTGACGGCCG ACAACGCATT CCGCGACCAG TGGATCGTGA CCTCGGGCCT GCACGCCGGC GACCGCGTGA TCGTGAGCGG CCTGCAGAGC GTGCATGCGG GCGCGCCGGT CAAGGTGGCC GCCGATACGC CGCAGACGCA GGGGAACGCG GTATCTGCGG CATCTGCGGC GTCCGCTGCC ACCGACACAT CCGCAGCCGG CACGGCCTCG CCCGTGTCGG CGCAACACTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A