BuviA.00107.c

Efflux transporter, RND family, MFP subunit

CENTER ID: BuviA.00107.c
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.c.A2.GE32427 Mature protein( BuviA.00107.c ) 34 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.c.A2.PW32347 Mature protein( BuviA.00107.c ) 34 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.2282
RefSeq: YP_001116032.1
UniProt: A4JJW4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAILRTTRSR IATAAIATLA VVGLGTFGAM RVNANAPDKA AAPLPEVDVA TVVPQTVTDW QSYSGRLEAV EKVDVRPQVS GTIVAVNFKD GALVKKGDVL FVIDPRPYQA ETDRAAAQLA AAQARNGYAQ SDWQRAQRLI GDNAIAKRDY DEKQNAAREA SANLKAAEAA LETARINLGY TRITAPVSGR VSRAEITVGN VVSAGAAATP LTTLVSVSPI YASFDADEQT YLQYINGARD GRKVPVELGL ANETGYSRSG VIDSVDNRLD TSSGTIRVRA RFDNADGTLV PGLYARVKVG GSAPHPALLV DDAAINTDQD KKFVFVVDQQ GRVSYREVQQ GMQHGNRRVI VSGLAAGDRV IVNGTQRVRP GEQVKPHMVP MTGGDDPSAP LADAAKPAAP AKAES
NT Sequence
ATGGCCATCC TACGCACCAC CCGCTCCCGA ATCGCCACCG CGGCGATCGC GACGCTCGCC GTCGTCGGTC TCGGCACGTT CGGCGCGATG CGCGTGAACG CGAACGCGCC CGACAAGGCC GCAGCGCCGC TGCCCGAAGT GGACGTCGCG ACCGTCGTGC CGCAAACCGT CACCGATTGG CAAAGCTACT CGGGCCGCCT CGAGGCGGTC GAGAAGGTCG ACGTGCGTCC GCAGGTGTCG GGCACCATCG TCGCGGTGAA CTTCAAGGAC GGCGCGCTCG TGAAGAAGGG CGACGTGCTG TTCGTGATCG ACCCGCGCCC GTACCAGGCC GAGACCGATC GCGCCGCCGC GCAACTCGCG GCCGCGCAGG CGCGCAACGG CTACGCGCAG AGCGACTGGC AGCGCGCGCA GCGGCTGATC GGCGACAACG CGATCGCGAA GCGCGACTAC GACGAGAAGC AGAACGCGGC GCGCGAGGCG AGCGCGAACC TGAAGGCGGC CGAAGCCGCG CTCGAAACGG CGCGCATCAA CCTCGGCTAT ACGCGCATCA CCGCGCCCGT GTCGGGCCGC GTGTCGCGCG CCGAGATCAC CGTGGGCAAC GTCGTGTCGG CGGGCGCCGC GGCGACGCCG CTGACGACGC TCGTATCGGT GTCGCCGATC TACGCGTCGT TCGACGCGGA CGAACAGACC TACCTGCAAT ACATCAACGG CGCGCGCGAC GGCCGCAAGG TGCCGGTCGA GCTCGGCCTC GCGAACGAAA CCGGCTATTC ACGCAGCGGC GTGATCGACT CGGTCGACAA CCGGCTCGAC ACGTCGTCCG GCACGATCCG CGTGCGCGCG CGCTTCGACA ACGCTGACGG CACGCTGGTG CCGGGCCTCT ACGCGCGCGT GAAGGTCGGC GGCAGCGCGC CGCACCCGGC ATTGCTCGTC GACGATGCGG CGATCAACAC CGACCAGGAC AAGAAGTTCG TGTTCGTCGT CGACCAGCAG GGCCGCGTGT CGTACCGCGA AGTGCAGCAG GGCATGCAGC ACGGCAACCG GCGCGTGATC GTGAGCGGGC TTGCCGCCGG CGATCGCGTG ATCGTGAACG GCACGCAGCG CGTGCGCCCG GGCGAGCAGG TCAAGCCGCA CATGGTGCCG ATGACGGGCG GCGACGATCC GTCCGCGCCG CTCGCGGACG CCGCGAAGCC GGCCGCACCG GCGAAGGCGG AATCG
Details for BuviA.00107.c.A2.GE32427
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 41kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MANAPDKAAA PLPEVDVATV VPQTVTDWQS YSGRLEAVEK VDVRPQVSGT IVAVNFKDGA LVKKGDVLFV IDPRPYQAET DRAAAQLAAA QARNGYAQSD WQRAQRLIGD NAIAKRDYDE KQNAAREASA NLKAAEAALE TARINLGYTR ITAPVSGRVS RAEITVGNVV SAGAAATPLT TLVSVSPIYA SFDADEQTYL QYINGARDGR KVPVELGLAN ETGYSRSGVI DSVDNRLDTS SGTIRVRARF DNADGTLVPG LYARVKVGGS APHPALLVDD AAINTDQDKK FVFVVDQQGR VSYREVQQGM QHGNRRVIVS GLAAGDRVIV NGTQRVRPGE QVKPHMVPMT GGDDPSAPLA DAXKPAAPAK A
Validated NT Sequence
ccgccttcgc cggtgcggcc ggcttcncgg cgtccgcgag cggcgcggac ggatcgtcgc cgcccgtcat cggcaccatg tgcggcttga cctgctcgcc cgggcgcacg cgctgcgtgc cgttcacgat cacgcgatcg ccggcggcaa gcccgctcac gatcacgcgc cggttgccgt gctgcatgcc ctgctgcact tcgcggtacg acacgcggcc ctgctggtcg acgacgaaca cgaacttctt gtcctggtcg gtgttgatcg ccgcatcgtc gacgagcaat gccgggtgcg gcgcgctgcc gccgaccttc acgcgcgcgt agaggcccgg caccagcgtg ccgtcagcgt tgtcgaagcg cgcgcgcacg cggatcgtgc cggacgacgt gtcgagccgg ttgtcgaccg agtcgatcac gccgctgcgt gaatagccgg tttcgttcgc gaggccgagc tcgaccggca ccttgcggcc gtcgcgcgcg ccgttgatgt attgcaggta ggtctgttcg tccgcgtcga acgacgcgta gatcggcgac accgatacga gcgtcgtcag cggcgtcgcc gcggcgcccg ccgacacgac gttgcccacg gtgatctcgg cgcgcgacac gcggcccgac acgggcgcgg tgatgcgcgt atagccgagg ttgatgcgcg ccgtttcgag cgcggcttcg gccgccttca ggttcgcgct cgcctcgcgc gccgcgttct gcttctcgtc gtagtcgcgc ttcgcgatcg cgttgtcgcc gatcagccgc tgcgcgcgct gccagtcgct ctgcgcgtag ccgttgcgcg cctgcgcggc cgcgagttgc gcggcggcgc gatcggtctc ggcctggtac gggcgcgggt cgatcacgaa cagcacgtcg cccttcttca cgagcgcgcc gtccttgaag ttcaccgcga cgatggtgcc cgacacctgc ggacgcacgt cgaccttctc gaccgcctcg aggcggcccg agtagctttg ccaatcggtg acggtttgcg gcacgacggt cgcgacgtcc acttcgggca gcggcgctgc ggccttgtcg ggcgcgttcg ccatcgaacc aggaccctgg gtctgagctt ccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANAPDKAA APLPEVDVAT VVPQTVTDWQ SYSGRLEAVE KVDVRPQVSG TIVAVNFKDG ALVKKGDVLF VIDPRPYQAE TDRAAAQLAA AQARNGYAQS DWQRAQRLIG DNAIAKRDYD EKQNAAREAS ANLKAAEAAL ETARINLGYT RITAPVSGRV SRAEITVGNV VSAGAAATPL TTLVSVSPIY ASFDADEQTY LQYINGARDG RKVPVELGLA NETGYSRSGV IDSVDNRLDT SSGTIRVRAR FDNADGTLVP GLYARVKVGG SAPHPALLVD DAAINTDQDK KFVFVVDQQG RVSYREVQQG MQHGNRRVIV SGLAAGDRVI VNGTQRVRPG EQVKPHMVPM TGGDDPSAPL ADAAKPAAPA KAES
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaacgcg cccgacaagg ccgcagcgcc gctgcccgaa gtggacgtcg cgaccgtcgt gccgcaaacc gtcaccgatt ggcaaagcta ctcgggccgc ctcgaggcgg tcgagaaggt cgacgtgcgt ccgcaggtgt cgggcaccat cgtcgcggtg aacttcaagg acggcgcgct cgtgaagaag ggcgacgtgc tgttcgtgat cgacccgcgc ccgtaccagg ccgagaccga tcgcgccgcc gcgcaactcg cggccgcgca ggcgcgcaac ggctacgcgc agagcgactg gcagcgcgcg cagcggctga tcggcgacaa cgcgatcgcg aagcgcgact acgacgagaa gcagaacgcg gcgcgcgagg cgagcgcgaa cctgaaggcg gccgaagccg cgctcgaaac ggcgcgcatc aacctcggct atacgcgcat caccgcgccc gtgtcgggcc gcgtgtcgcg cgccgagatc accgtgggca acgtcgtgtc ggcgggcgcc gcggcgacgc cgctgacgac gctcgtatcg gtgtcgccga tctacgcgtc gttcgacgcg gacgaacaga cctacctgca atacatcaac ggcgcgcgcg acggccgcaa ggtgccggtc gagctcggcc tcgcgaacga aaccggctat tcacgcagcg gcgtgatcga ctcggtcgac aaccggctcg acacgtcgtc cggcacgatc cgcgtgcgcg cgcgcttcga caacgctgac ggcacgctgg tgccgggcct ctacgcgcgc gtgaaggtcg gcggcagcgc gccgcacccg gcattgctcg tcgacgatgc ggcgatcaac accgaccagg acaagaagtt cgtgttcgtc gtcgaccagc agggccgcgt gtcgtaccgc gaagtgcagc agggcatgca gcacggcaac cggcgcgtga tcgtgagcgg gcttgccgcc ggcgatcgcg tgatcgtgaa cggcacgcag cgcgtgcgcc cgggcgagca ggtcaagccg cacatggtgc cgatgacggg cggcgacgat ccgtccgcgc cgctcgcgga cgccgcgaag ccggccgcac cggcgaaggc ggaatcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuviA.00107.c.A2.PW32347
PURIFICATION DATe: 12/16/2011
CONCENTRATION: 31.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MANAPDKAAA PLPEVDVATV VPQTVTDWQS YSGRLEAVEK VDVRPQVSGT IVAVNFKDGA LVKKGDVLFV IDPRPYQAET DRAAAQLAAA QARNGYAQSD WQRAQRLIGD NAIAKRDYDE KQNAAREASA NLKAAEAALE TARINLGYTR ITAPVSGRVS RAEITVGNVV SAGAAATPLT TLVSVSPIYA SFDADEQTYL QYINGARDGR KVPVELGLAN ETGYSRSGVI DSVDNRLDTS SGTIRVRARF DNADGTLVPG LYARVKVGGS APHPALLVDD AAINTDQDKK FVFVVDQQGR VSYREVQQGM QHGNRRVIVS GLAAGDRVIV NGTQRVRPGE QVKPHMVPMT GGDDPSAPLA DAXKPAAPAK A
Validated NT Sequence
ccgccttcgc cggtgcggcc ggcttcncgg cgtccgcgag cggcgcggac ggatcgtcgc cgcccgtcat cggcaccatg tgcggcttga cctgctcgcc cgggcgcacg cgctgcgtgc cgttcacgat cacgcgatcg ccggcggcaa gcccgctcac gatcacgcgc cggttgccgt gctgcatgcc ctgctgcact tcgcggtacg acacgcggcc ctgctggtcg acgacgaaca cgaacttctt gtcctggtcg gtgttgatcg ccgcatcgtc gacgagcaat gccgggtgcg gcgcgctgcc gccgaccttc acgcgcgcgt agaggcccgg caccagcgtg ccgtcagcgt tgtcgaagcg cgcgcgcacg cggatcgtgc cggacgacgt gtcgagccgg ttgtcgaccg agtcgatcac gccgctgcgt gaatagccgg tttcgttcgc gaggccgagc tcgaccggca ccttgcggcc gtcgcgcgcg ccgttgatgt attgcaggta ggtctgttcg tccgcgtcga acgacgcgta gatcggcgac accgatacga gcgtcgtcag cggcgtcgcc gcggcgcccg ccgacacgac gttgcccacg gtgatctcgg cgcgcgacac gcggcccgac acgggcgcgg tgatgcgcgt atagccgagg ttgatgcgcg ccgtttcgag cgcggcttcg gccgccttca ggttcgcgct cgcctcgcgc gccgcgttct gcttctcgtc gtagtcgcgc ttcgcgatcg cgttgtcgcc gatcagccgc tgcgcgcgct gccagtcgct ctgcgcgtag ccgttgcgcg cctgcgcggc cgcgagttgc gcggcggcgc gatcggtctc ggcctggtac gggcgcgggt cgatcacgaa cagcacgtcg cccttcttca cgagcgcgcc gtccttgaag ttcaccgcga cgatggtgcc cgacacctgc ggacgcacgt cgaccttctc gaccgcctcg aggcggcccg agtagctttg ccaatcggtg acggtttgcg gcacgacggt cgcgacgtcc acttcgggca gcggcgctgc ggccttgtcg ggcgcgttcg ccatcgaacc aggaccctgg gtctgagctt ccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANAPDKAA APLPEVDVAT VVPQTVTDWQ SYSGRLEAVE KVDVRPQVSG TIVAVNFKDG ALVKKGDVLF VIDPRPYQAE TDRAAAQLAA AQARNGYAQS DWQRAQRLIG DNAIAKRDYD EKQNAAREAS ANLKAAEAAL ETARINLGYT RITAPVSGRV SRAEITVGNV VSAGAAATPL TTLVSVSPIY ASFDADEQTY LQYINGARDG RKVPVELGLA NETGYSRSGV IDSVDNRLDT SSGTIRVRAR FDNADGTLVP GLYARVKVGG SAPHPALLVD DAAINTDQDK KFVFVVDQQG RVSYREVQQG MQHGNRRVIV SGLAAGDRVI VNGTQRVRPG EQVKPHMVPM TGGDDPSAPL ADAAKPAAPA KAES
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGAACGCG CCCGACAAGG CCGCAGCGCC GCTGCCCGAA GTGGACGTCG CGACCGTCGT GCCGCAAACC GTCACCGATT GGCAAAGCTA CTCGGGCCGC CTCGAGGCGG TCGAGAAGGT CGACGTGCGT CCGCAGGTGT CGGGCACCAT CGTCGCGGTG AACTTCAAGG ACGGCGCGCT CGTGAAGAAG GGCGACGTGC TGTTCGTGAT CGACCCGCGC CCGTACCAGG CCGAGACCGA TCGCGCCGCC GCGCAACTCG CGGCCGCGCA GGCGCGCAAC GGCTACGCGC AGAGCGACTG GCAGCGCGCG CAGCGGCTGA TCGGCGACAA CGCGATCGCG AAGCGCGACT ACGACGAGAA GCAGAACGCG GCGCGCGAGG CGAGCGCGAA CCTGAAGGCG GCCGAAGCCG CGCTCGAAAC GGCGCGCATC AACCTCGGCT ATACGCGCAT CACCGCGCCC GTGTCGGGCC GCGTGTCGCG CGCCGAGATC ACCGTGGGCA ACGTCGTGTC GGCGGGCGCC GCGGCGACGC CGCTGACGAC GCTCGTATCG GTGTCGCCGA TCTACGCGTC GTTCGACGCG GACGAACAGA CCTACCTGCA ATACATCAAC GGCGCGCGCG ACGGCCGCAA GGTGCCGGTC GAGCTCGGCC TCGCGAACGA AACCGGCTAT TCACGCAGCG GCGTGATCGA CTCGGTCGAC AACCGGCTCG ACACGTCGTC CGGCACGATC CGCGTGCGCG CGCGCTTCGA CAACGCTGAC GGCACGCTGG TGCCGGGCCT CTACGCGCGC GTGAAGGTCG GCGGCAGCGC GCCGCACCCG GCATTGCTCG TCGACGATGC GGCGATCAAC ACCGACCAGG ACAAGAAGTT CGTGTTCGTC GTCGACCAGC AGGGCCGCGT GTCGTACCGC GAAGTGCAGC AGGGCATGCA GCACGGCAAC CGGCGCGTGA TCGTGAGCGG GCTTGCCGCC GGCGATCGCG TGATCGTGAA CGGCACGCAG CGCGTGCGCC CGGGCGAGCA GGTCAAGCCG CACATGGTGC CGATGACGGG CGGCGACGAT CCGTCCGCGC CGCTCGCGGA CGCCGCGAAG CCGGCCGCAC CGGCGAAGGC GGAATCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA