BuviA.00107.b

Efflux transporter, RND family, MFP subunit

CENTER ID: BuviA.00107.b
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.b.A2.GE32426 Mature protein( BuviA.00107.b ) 24 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00107.b.A2.PS01325 Mature protein( BuviA.00107.b ) 24 418

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.6968
RefSeq: YP_001120549.1
UniProt: A4JHG1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVERVPYRL LTVATAAVFL AACGKKESAP PPQTPEVGVV TVQPQAVPIY TELPGRTSAF LVAQVRARVD GIVLRREFVE GSDVKAGQRL YKIDPAPYLA QLNSAKATLA KAQANLVTQN ALVARYKVLV AANAVSKQDY DNAVATQGQA AADVAAGKAA VETAQINLGY TDVVSPISGR VGISQVTPGA YVQASQATLM STVQQLDPVY VDLTQSSLEG LKLRQDVQSG RLKTSGPGAA KVSLILEDGK TYPVPGKLQF SDVTVDQTTG SVTIRAVFPN PNRVLLPGMF VRARIEEGVN ENAFLVPQIG VTHDQKGQAI AMVVNASNKV EPRPLKTTGM NGQNWIVEGG LNAGDRVIVQ GGDKARPGTT VKTVAAQLPA ADAASGAAAS AAPAAAGSGA AAASGAAPAS AAAASSAQ
NT Sequence
ATGCGCGTCG AACGGGTTCC ATACCGCTTA CTCACTGTCG CGACGGCCGC CGTTTTCCTG GCCGCGTGCG GGAAAAAAGA ATCGGCACCG CCGCCGCAAA CGCCGGAAGT CGGCGTCGTC ACCGTCCAAC CGCAAGCCGT ACCGATCTAC ACCGAACTGC CGGGCCGCAC CAGTGCGTTC CTCGTCGCGC AGGTCCGCGC GCGGGTCGAC GGCATCGTGC TGCGCCGTGA ATTCGTCGAA GGCAGCGACG TCAAGGCCGG CCAGCGCCTC TACAAGATCG ATCCGGCGCC GTACCTCGCG CAACTGAACA GCGCGAAGGC GACGCTCGCG AAGGCGCAGG CGAACCTCGT CACGCAGAAC GCGCTGGTCG CGCGCTACAA GGTGCTAGTG GCCGCCAACG CGGTCAGCAA GCAGGACTAC GACAACGCGG TGGCCACGCA GGGCCAGGCC GCGGCCGACG TCGCAGCCGG CAAGGCAGCG GTCGAAACCG CGCAGATCAA CCTCGGCTAT ACGGACGTCG TGTCGCCGAT CAGCGGCCGC GTCGGCATCT CGCAGGTGAC GCCGGGCGCT TACGTGCAGG CGAGCCAGGC GACGCTGATG TCGACGGTGC AGCAGCTCGA TCCGGTCTAC GTCGACCTGA CGCAATCGAG CCTTGAGGGG CTGAAGCTGC GCCAGGACGT GCAGAGCGGC CGCCTGAAGA CGAGCGGCCC CGGCGCGGCG AAGGTGTCGC TGATCCTCGA GGACGGCAAG ACCTACCCGG TGCCCGGCAA GCTGCAGTTC TCCGACGTGA CGGTCGACCA GACGACCGGC TCGGTCACGA TCCGCGCGGT GTTCCCGAAC CCGAACCGCG TGCTGCTGCC GGGCATGTTC GTGCGCGCCC GCATCGAGGA AGGCGTGAAC GAGAACGCGT TCCTGGTGCC GCAGATCGGC GTCACGCACG ACCAGAAGGG CCAGGCGATC GCGATGGTGG TGAACGCGAG CAACAAGGTC GAGCCGCGTC CGCTGAAGAC GACCGGCATG AACGGTCAGA ACTGGATCGT CGAAGGCGGG CTGAACGCGG GCGACCGCGT GATCGTCCAG GGCGGCGACA AGGCGCGTCC GGGCACGACG GTGAAGACGG TCGCCGCGCA GCTGCCGGCA GCCGATGCCG CATCGGGTGC AGCCGCATCC GCCGCGCCGG CCGCCGCCGG TTCCGGTGCC GCAGCCGCGT CGGGCGCCGC GCCGGCCAGC GCCGCCGCTG CGTCGAGCGC GCAA
Details for BuviA.00107.b.A2.GE32426
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGKKESAPPP QTPEVGVVTV QPQAVPIYTE LPGRTSAFLV AQVRARVDGI VLRREFVEGS DVKAGQRLYK IDPAPYLAQL NSAKATLAKA QANLVTQNAL VARYKVLVAA NAVSKQDYDN AVATQGQAAA DVAAGKAAVE TAQINLGYTD VVSPISGRVG ISQVTPGAYV QASQATLMST VQQLDPVYVD LTQSSLEGLK LRQDVQSGRL KTSGPGAAKV SLILEDGKTY PVPGKLQFSD VTVDQTTGSV TIRAVFPNPN RVLLPGMFVR ARIEEGVNEN AFLVPQIGVT HDQKGQAIAM VVNASNKVEP RPLKTTGMNG QNWIVEGGLN AGDRVIVQGG DKARPGTTVK TVAAQLPAAD AASGAAASAA PAAAGSGAAA ASGAAPASAA AASSA
Validated NT Sequence
tgcgcgctcg acgcagcggc ggcgctggcc ggcgcggcgc ccgacgcggc tgcggcaccg gaaccggcgg cggccggcgc ggcggatgcg gctgcacccg atgcggcatc ggctgccggc agctgcgcgg cgaccgtctt caccgtcgtg cccggacgcg ccttgtcgcc gccctggacg atcacgcggt cgcccgcgtt cagcccgcct tcgacgatcc agttctgacc gttcatgccg gtcgtcttca gcggacgcgg ctcgaccttg ttgctcgcgt tcaccaccat cgcgatcgcc tggcccttct ggtcgtgcgt gacgccgatc tgcggcacca ggaacgcgtt ctcgttcacg ccttcctcga tgcgggcgcg cacgaacatg cccggcagca gcacgcggtt cgggttcggg aacaccgcgc ggatcgtgac cgagccggtc gtctggtcga ccgtcacgtc ggagaactgc agcttgccgg gcaccgggta ggtcttgccg tcctcgagga tcagcgacac cttcgccgcg ccggggccgc tcgtcttcag gcggccgctc tgcacgtcct ggcgcagctt cagcccctca aggctcgatt gcgtcaggtc gacgtagacc ggatcgagct gctgcaccgt cgacatcagc gtcgcctggc tcgcctgcac gtaagcgccc ggcgtcacct gcgagatgcc gacgcggccg ctgatcggcg acacgacgtc cgtatagccg aggttgatct gcgcggtttc gaccgctgcc ttgccggctg cgacgtcggc cgcggcctgg ccctgcgtgg ccaccgcgtt gtcgtagtcc tgcttgctga ccgcgttggc ggccactagc accttgtagc gcgcgaccag cgcgttctgc gtgacgaggt tcgcctgcgc cttcgcgagc gtcgccttcg cgctgttcag ttgcgcgagg tacggcgccg gatcgatctt gtagaggcgc tggccggcct tgacgtcgct gccttcgacg aattcacggc gcagcacgat gccgtcgacc cgcgcgcgga cctgcgcgac gaggaacgca ctggtgcggc ccggcagttc ggtgtagatc ggtacggctt gcggttggac ggtgacgacg ccgacttccg gcgtttgcgg cggcggtgcc gattcttttt tccccatcga accaggaccc tgggtctgag cttccagggt ac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGKKESAPP PQTPEVGVVT VQPQAVPIYT ELPGRTSAFL VAQVRARVDG IVLRREFVEG SDVKAGQRLY KIDPAPYLAQ LNSAKATLAK AQANLVTQNA LVARYKVLVA ANAVSKQDYD NAVATQGQAA ADVAAGKAAV ETAQINLGYT DVVSPISGRV GISQVTPGAY VQASQATLMS TVQQLDPVYV DLTQSSLEGL KLRQDVQSGR LKTSGPGAAK VSLILEDGKT YPVPGKLQFS DVTVDQTTGS VTIRAVFPNP NRVLLPGMFV RARIEEGVNE NAFLVPQIGV THDQKGQAIA MVVNASNKVE PRPLKTTGMN GQNWIVEGGL NAGDRVIVQG GDKARPGTTV KTVAAQLPAA DAASGAAASA APAAAGSGAA AASGAAPASA AAASSAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggaaaaaa gaatcggcac cgccgccgca aacgccggaa gtcggcgtcg tcaccgtcca accgcaagcc gtaccgatct acaccgaact gccgggccgc accagtgcgt tcctcgtcgc gcaggtccgc gcgcgggtcg acggcatcgt gctgcgccgt gaattcgtcg aaggcagcga cgtcaaggcc ggccagcgcc tctacaagat cgatccggcg ccgtacctcg cgcaactgaa cagcgcgaag gcgacgctcg cgaaggcgca ggcgaacctc gtcacgcaga acgcgctggt cgcgcgctac aaggtgctag tggccgccaa cgcggtcagc aagcaggact acgacaacgc ggtggccacg cagggccagg ccgcggccga cgtcgcagcc ggcaaggcag cggtcgaaac cgcgcagatc aacctcggct atacggacgt cgtgtcgccg atcagcggcc gcgtcggcat ctcgcaggtg acgccgggcg cttacgtgca ggcgagccag gcgacgctga tgtcgacggt gcagcagctc gatccggtct acgtcgacct gacgcaatcg agccttgagg ggctgaagct gcgccaggac gtgcagagcg gccgcctgaa gacgagcggc cccggcgcgg cgaaggtgtc gctgatcctc gaggacggca agacctaccc ggtgcccggc aagctgcagt tctccgacgt gacggtcgac cagacgaccg gctcggtcac gatccgcgcg gtgttcccga acccgaaccg cgtgctgctg ccgggcatgt tcgtgcgcgc ccgcatcgag gaaggcgtga acgagaacgc gttcctggtg ccgcagatcg gcgtcacgca cgaccagaag ggccaggcga tcgcgatggt ggtgaacgcg agcaacaagg tcgagccgcg tccgctgaag acgaccggca tgaacggtca gaactggatc gtcgaaggcg ggctgaacgc gggcgaccgc gtgatcgtcc agggcggcga caaggcgcgt ccgggcacga cggtgaagac ggtcgccgcg cagctgccgg cagccgatgc cgcatcgggt gcagccgcat ccgccgcgcc ggccgccgcc ggttccggtg ccgcagccgc gtcgggcgcc gcgccggcca gcgccgccgc tgcgtcgagc gcgcaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuviA.00107.b.A2.PS01325
PURIFICATION DATe: 12/29/2011
CONCENTRATION: 39.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MGKKESAPPP QTPEVGVVTV QPQAVPIYTE LPGRTSAFLV AQVRARVDGI VLRREFVEGS DVKAGQRLYK IDPAPYLAQL NSAKATLAKA QANLVTQNAL VARYKVLVAA NAVSKQDYDN AVATQGQAAA DVAAGKAAVE TAQINLGYTD VVSPISGRVG ISQVTPGAYV QASQATLMST VQQLDPVYVD LTQSSLEGLK LRQDVQSGRL KTSGPGAAKV SLILEDGKTY PVPGKLQFSD VTVDQTTGSV TIRAVFPNPN RVLLPGMFVR ARIEEGVNEN AFLVPQIGVT HDQKGQAIAM VVNASNKVEP RPLKTTGMNG QNWIVEGGLN AGDRVIVQGG DKARPGTTVK TVAAQLPAAD AASGAAASAA PAAAGSGAAA ASGAAPASAA AASSA
Validated NT Sequence
tgcgcgctcg acgcagcggc ggcgctggcc ggcgcggcgc ccgacgcggc tgcggcaccg gaaccggcgg cggccggcgc ggcggatgcg gctgcacccg atgcggcatc ggctgccggc agctgcgcgg cgaccgtctt caccgtcgtg cccggacgcg ccttgtcgcc gccctggacg atcacgcggt cgcccgcgtt cagcccgcct tcgacgatcc agttctgacc gttcatgccg gtcgtcttca gcggacgcgg ctcgaccttg ttgctcgcgt tcaccaccat cgcgatcgcc tggcccttct ggtcgtgcgt gacgccgatc tgcggcacca ggaacgcgtt ctcgttcacg ccttcctcga tgcgggcgcg cacgaacatg cccggcagca gcacgcggtt cgggttcggg aacaccgcgc ggatcgtgac cgagccggtc gtctggtcga ccgtcacgtc ggagaactgc agcttgccgg gcaccgggta ggtcttgccg tcctcgagga tcagcgacac cttcgccgcg ccggggccgc tcgtcttcag gcggccgctc tgcacgtcct ggcgcagctt cagcccctca aggctcgatt gcgtcaggtc gacgtagacc ggatcgagct gctgcaccgt cgacatcagc gtcgcctggc tcgcctgcac gtaagcgccc ggcgtcacct gcgagatgcc gacgcggccg ctgatcggcg acacgacgtc cgtatagccg aggttgatct gcgcggtttc gaccgctgcc ttgccggctg cgacgtcggc cgcggcctgg ccctgcgtgg ccaccgcgtt gtcgtagtcc tgcttgctga ccgcgttggc ggccactagc accttgtagc gcgcgaccag cgcgttctgc gtgacgaggt tcgcctgcgc cttcgcgagc gtcgccttcg cgctgttcag ttgcgcgagg tacggcgccg gatcgatctt gtagaggcgc tggccggcct tgacgtcgct gccttcgacg aattcacggc gcagcacgat gccgtcgacc cgcgcgcgga cctgcgcgac gaggaacgca ctggtgcggc ccggcagttc ggtgtagatc ggtacggctt gcggttggac ggtgacgacg ccgacttccg gcgtttgcgg cggcggtgcc gattcttttt tccccatcga accaggaccc tgggtctgag cttccagggt ac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGKKESAPP PQTPEVGVVT VQPQAVPIYT ELPGRTSAFL VAQVRARVDG IVLRREFVEG SDVKAGQRLY KIDPAPYLAQ LNSAKATLAK AQANLVTQNA LVARYKVLVA ANAVSKQDYD NAVATQGQAA ADVAAGKAAV ETAQINLGYT DVVSPISGRV GISQVTPGAY VQASQATLMS TVQQLDPVYV DLTQSSLEGL KLRQDVQSGR LKTSGPGAAK VSLILEDGKT YPVPGKLQFS DVTVDQTTGS VTIRAVFPNP NRVLLPGMFV RARIEEGVNE NAFLVPQIGV THDQKGQAIA MVVNASNKVE PRPLKTTGMN GQNWIVEGGL NAGDRVIVQG GDKARPGTTV KTVAAQLPAA DAASGAAASA APAAAGSGAA AASGAAPASA AAASSAQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGAAAAAA GAATCGGCAC CGCCGCCGCA AACGCCGGAA GTCGGCGTCG TCACCGTCCA ACCGCAAGCC GTACCGATCT ACACCGAACT GCCGGGCCGC ACCAGTGCGT TCCTCGTCGC GCAGGTCCGC GCGCGGGTCG ACGGCATCGT GCTGCGCCGT GAATTCGTCG AAGGCAGCGA CGTCAAGGCC GGCCAGCGCC TCTACAAGAT CGATCCGGCG CCGTACCTCG CGCAACTGAA CAGCGCGAAG GCGACGCTCG CGAAGGCGCA GGCGAACCTC GTCACGCAGA ACGCGCTGGT CGCGCGCTAC AAGGTGCTAG TGGCCGCCAA CGCGGTCAGC AAGCAGGACT ACGACAACGC GGTGGCCACG CAGGGCCAGG CCGCGGCCGA CGTCGCAGCC GGCAAGGCAG CGGTCGAAAC CGCGCAGATC AACCTCGGCT ATACGGACGT CGTGTCGCCG ATCAGCGGCC GCGTCGGCAT CTCGCAGGTG ACGCCGGGCG CTTACGTGCA GGCGAGCCAG GCGACGCTGA TGTCGACGGT GCAGCAGCTC GATCCGGTCT ACGTCGACCT GACGCAATCG AGCCTTGAGG GGCTGAAGCT GCGCCAGGAC GTGCAGAGCG GCCGCCTGAA GACGAGCGGC CCCGGCGCGG CGAAGGTGTC GCTGATCCTC GAGGACGGCA AGACCTACCC GGTGCCCGGC AAGCTGCAGT TCTCCGACGT GACGGTCGAC CAGACGACCG GCTCGGTCAC GATCCGCGCG GTGTTCCCGA ACCCGAACCG CGTGCTGCTG CCGGGCATGT TCGTGCGCGC CCGCATCGAG GAAGGCGTGA ACGAGAACGC GTTCCTGGTG CCGCAGATCG GCGTCACGCA CGACCAGAAG GGCCAGGCGA TCGCGATGGT GGTGAACGCG AGCAACAAGG TCGAGCCGCG TCCGCTGAAG ACGACCGGCA TGAACGGTCA GAACTGGATC GTCGAAGGCG GGCTGAACGC GGGCGACCGC GTGATCGTCC AGGGCGGCGA CAAGGCGCGT CCGGGCACGA CGGTGAAGAC GGTCGCCGCG CAGCTGCCGG CAGCCGATGC CGCATCGGGT GCAGCCGCAT CCGCCGCGCC GGCCGCCGCC GGTTCCGGTG CCGCAGCCGC GTCGGGCGCC GCGCCGGCCA GCGCCGCCGC TGCGTCGAGC GCGCAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA