BuviA.00104.b
Beta-lactamase
| CENTER ID: | BuviA.00104.b |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00104.b.B2.GE39147 | Mature protein( BuviA.00104.b ) | 25 | 299 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00104.b.B2.PS02438 | Mature protein( BuviA.00104.b ) | 25 | 299 |
Structures
5HX9
DEPOSITED: 1/29/2016
DETERMINATION: XRay
CLONE: BuviA.00104.b.B2.GE39147
PROTEIN: BuviA.00104.b.B2.PS02438
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_143180 |
| PATRIC ID: | fig|269482.11.peg.3867 |
| RefSeq: | YP_001117541.1 |
| UniProt: | A4JP73 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDHSPTRRSL LLAAVSAPFV AACTSAPIAD QGRAHVARSE LAALDKASNG RLGVAALDTS NGTRIAHHAR ERFPLCGTYA VVAAAAILAR ASLDASLLPR RILYRRYEVV AGSPVTESHV DTGMTIAQLC AAMLQSGDKG AGNLLMNVLG GPQAVTAFAH ESGDTVFRLD RWEPELNRAA PGDERDTSTP VAMVDTLQRL LLGDTLQQAQ RAQLTDWMTA GAPDATGIAA GVPPGSRVAA KRGTGGYGTT TEVAVVWPPS RAPIVLAVSF TQAQADAAAR ADVVASAARI ATGALTATA
NT Sequence
ATGGACCATT CTCCGACTCG TCGTTCGCTG TTGCTCGCCG CCGTGTCCGC GCCGTTCGTC GCCGCGTGCA CGTCGGCTCC CATTGCCGAT CAGGGGCGCG CGCATGTTGC CCGCAGCGAG CTCGCCGCAC TCGATAAGGC GTCGAACGGC CGGCTTGGCG TCGCCGCGCT CGACACGTCG AACGGCACGC GCATCGCGCA TCATGCGCGC GAGCGCTTCC CACTGTGCGG CACCTATGCG GTCGTCGCGG CCGCCGCGAT CCTCGCGCGT GCGTCGCTCG ATGCATCGCT GCTGCCGCGT CGCATCCTGT ATCGCCGTTA CGAAGTCGTG GCCGGCTCGC CGGTGACGGA GAGCCACGTC GACACGGGCA TGACGATCGC GCAACTCTGC GCCGCAATGC TGCAGTCGGG TGACAAGGGC GCGGGGAACC TGCTGATGAA CGTGCTCGGC GGCCCGCAGG CCGTCACGGC GTTCGCGCAC GAAAGCGGCG ACACCGTGTT CCGGCTCGAT CGCTGGGAGC CCGAGCTGAA CCGTGCCGCG CCCGGCGACG AGCGCGACAC GTCGACGCCG GTTGCGATGG TCGATACGCT GCAGCGGCTG CTGCTCGGCG ACACGCTGCA GCAAGCGCAG CGCGCGCAGC TCACCGACTG GATGACGGCC GGCGCGCCCG ACGCGACCGG CATCGCGGCC GGCGTGCCGC CGGGCTCGCG CGTGGCGGCC AAGCGCGGCA CCGGCGGTTA CGGCACGACG ACCGAGGTCG CGGTGGTGTG GCCGCCGTCG CGCGCGCCGA TCGTGCTCGC GGTGTCGTTC ACGCAAGCGC AGGCCGACGC CGCTGCGCGC GCGGACGTCG TCGCGTCGGC GGCGCGCATC GCGACCGGAG CGCTCACCGC CACGGCC
Details for BuviA.00104.b.B2.GE39147
| HARVESTED ON: | 5/6/2015 |
| SEQUENCED ON: | 5/6/2015 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 59 |
Validated AA Sequence
MTIAQLCAAM LQSGDKGAGN LLMNVLGGPQ AVTAFAHESG DTVFRLDRWE PELNRAAPGD ERDTSTPVAM VDTLQRLLLG DTLQQAQRAQ LTDWMTAGAP DATGIAAGVP PGSRVAAKRG TGGYGTTTEV AVVWPPSRAP IVLAVSFTQA QADAAARADV VASAARI
Validated NT Sequence
cncgatgcgc gccgccgacg cgacgacgtc cgcgcgcgca gcggcgtcgg cctgcgcttg cgtgaacgac accgcgagca cgatcggcgc gcgcgacggc ggccacacca ccgcgacctc ggtcgtcgtg ccgtaaccgc cggtgccgcg cttggccgcc acgcgcgagc ccggcggcac gccggccgcg atgccggtcg cgtcgggcgc gccggccgtc atccagtcgg tgagctgcgc gcgctgcgct tgctgcagcg tgtcgccgag cagcagccgc tgcagcgtat cgaccatcgc aaccggcgtc gacgtgtcgc gctcgtcgcc gggcgcggca cggttcagct cgggctccca gcgatcgagc cggaacacgg tgtcgccgct ttcgtgcgcg aacgccgtga cggcctgcgg gccgccgagc acgttcatca gcaggttccc cgcgcccttg tcacccgact gcagcattgc ggcgcagagt tgcgcgatcg tcatgcccgt gtcgacgtgg ctctccgtca ccggcgagcc ggccacgact tcgtaacggc gatacaggat gcgacgcggc agcagcgatg catcgagcga cnnnngcgcg aggatcgcgg cggccgcgac gaccgcatag gtgccgcaca gtgggaagcg ctcgcgcgca tgatgcgcga nnnnngtgcc gtnnnnnnnn nnnnnnncgg nnacgnnnng nnnnncntnn nnnnnnnnnn nnnntgcgnn nnncnnnnng cnnnnannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng g
Expected Protein Sequence
MAHHHHHHSA PIADQGRAHV ARSELAALDK ASNGRLGVAA LDTSNGTRIA HHARERFPLC GTYAVVAAAA ILARASLDAS LLPRRILYRR YEVVAGSPVT ESHVDTGMTI AQLCAAMLQS GDKGAGNLLM NVLGGPQAVT AFAHESGDTV FRLDRWEPEL NRAAPGDERD TSTPVAMVDT LQRLLLGDTL QQAQRAQLTD WMTAGAPDAT GIAAGVPPGS RVAAKRGTGG YGTTTEVAVV WPPSRAPIVL AVSFTQAQAD AAARADVVAS AARIATGALT ATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcggctc ccattgccga tcaggggcgc gcgcatgttg cccgcagcga gctcgccgca ctcgataagg cgtcgaacgg ccggcttggc gtcgccgcgc tcgacacgtc gaacggcacg cgcatcgcgc atcatgcgcg cgagcgcttc ccactgtgcg gcacctatgc ggtcgtcgcg gccgccgcga tcctcgcgcg tgcgtcgctc gatgcatcgc tgctgccgcg tcgcatcctg tatcgccgtt acgaagtcgt ggccggctcg ccggtgacgg agagccacgt cgacacgggc atgacgatcg cgcaactctg cgccgcaatg ctgcagtcgg gtgacaaggg cgcggggaac ctgctgatga acgtgctcgg cggcccgcag gccgtcacgg cgttcgcgca cgaaagcggc gacaccgtgt tccggctcga tcgctgggag cccgagctga accgtgccgc gcccggcgac gagcgcgaca cgtcgacgcc ggttgcgatg gtcgatacgc tgcagcggct gctgctcggc gacacgctgc agcaagcgca gcgcgcgcag ctcaccgact ggatgacggc cggcgcgccc gacgcgaccg gcatcgcggc cggcgtgccg ccgggctcgc gcgtggcggc caagcgcggc accggcggtt acggcacgac gaccgaggtc gcggtggtgt ggccgccgtc gcgcgcgccg atcgtgctcg cggtgtcgtt cacgcaagcg caggccgacg ccgctgcgcg cgcggacgtc gtcgcgtcgg cggcgcgcat cgcgaccgga gcgctcaccg ccacggcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuviA.00104.b.B2.PS02438
| PURIFICATION DATe: | 7/1/2015 |
| CONCENTRATION: | 8.39mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 59 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTIAQLCAAM LQSGDKGAGN LLMNVLGGPQ AVTAFAHESG DTVFRLDRWE PELNRAAPGD ERDTSTPVAM VDTLQRLLLG DTLQQAQRAQ LTDWMTAGAP DATGIAAGVP PGSRVAAKRG TGGYGTTTEV AVVWPPSRAP IVLAVSFTQA QADAAARADV VASAARI
Validated NT Sequence
cncgatgcgc gccgccgacg cgacgacgtc cgcgcgcgca gcggcgtcgg cctgcgcttg cgtgaacgac accgcgagca cgatcggcgc gcgcgacggc ggccacacca ccgcgacctc ggtcgtcgtg ccgtaaccgc cggtgccgcg cttggccgcc acgcgcgagc ccggcggcac gccggccgcg atgccggtcg cgtcgggcgc gccggccgtc atccagtcgg tgagctgcgc gcgctgcgct tgctgcagcg tgtcgccgag cagcagccgc tgcagcgtat cgaccatcgc aaccggcgtc gacgtgtcgc gctcgtcgcc gggcgcggca cggttcagct cgggctccca gcgatcgagc cggaacacgg tgtcgccgct ttcgtgcgcg aacgccgtga cggcctgcgg gccgccgagc acgttcatca gcaggttccc cgcgcccttg tcacccgact gcagcattgc ggcgcagagt tgcgcgatcg tcatgcccgt gtcgacgtgg ctctccgtca ccggcgagcc ggccacgact tcgtaacggc gatacaggat gcgacgcggc agcagcgatg catcgagcga cnnnngcgcg aggatcgcgg cggccgcgac gaccgcatag gtgccgcaca gtgggaagcg ctcgcgcgca tgatgcgcga nnnnngtgcc gtnnnnnnnn nnnnnnncgg nnacgnnnng nnnnncntnn nnnnnnnnnn nnnntgcgnn nnncnnnnng cnnnnannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng g
Expressed Protein Sequence
MAHHHHHHSA PIADQGRAHV ARSELAALDK ASNGRLGVAA LDTSNGTRIA HHARERFPLC GTYAVVAAAA ILARASLDAS LLPRRILYRR YEVVAGSPVT ESHVDTGMTI AQLCAAMLQS GDKGAGNLLM NVLGGPQAVT AFAHESGDTV FRLDRWEPEL NRAAPGDERD TSTPVAMVDT LQRLLLGDTL QQAQRAQLTD WMTAGAPDAT GIAAGVPPGS RVAAKRGTGG YGTTTEVAVV WPPSRAPIVL AVSFTQAQAD AAARADVVAS AARIATGALT ATA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCGGCTC CCATTGCCGA TCAGGGGCGC GCGCATGTTG CCCGCAGCGA GCTCGCCGCA CTCGATAAGG CGTCGAACGG CCGGCTTGGC GTCGCCGCGC TCGACACGTC GAACGGCACG CGCATCGCGC ATCATGCGCG CGAGCGCTTC CCACTGTGCG GCACCTATGC GGTCGTCGCG GCCGCCGCGA TCCTCGCGCG TGCGTCGCTC GATGCATCGC TGCTGCCGCG TCGCATCCTG TATCGCCGTT ACGAAGTCGT GGCCGGCTCG CCGGTGACGG AGAGCCACGT CGACACGGGC ATGACGATCG CGCAACTCTG CGCCGCAATG CTGCAGTCGG GTGACAAGGG CGCGGGGAAC CTGCTGATGA ACGTGCTCGG CGGCCCGCAG GCCGTCACGG CGTTCGCGCA CGAAAGCGGC GACACCGTGT TCCGGCTCGA TCGCTGGGAG CCCGAGCTGA ACCGTGCCGC GCCCGGCGAC GAGCGCGACA CGTCGACGCC GGTTGCGATG GTCGATACGC TGCAGCGGCT GCTGCTCGGC GACACGCTGC AGCAAGCGCA GCGCGCGCAG CTCACCGACT GGATGACGGC CGGCGCGCCC GACGCGACCG GCATCGCGGC CGGCGTGCCG CCGGGCTCGC GCGTGGCGGC CAAGCGCGGC ACCGGCGGTT ACGGCACGAC GACCGAGGTC GCGGTGGTGT GGCCGCCGTC GCGCGCGCCG ATCGTGCTCG CGGTGTCGTT CACGCAAGCG CAGGCCGACG CCGCTGCGCG CGCGGACGTC GTCGCGTCGG CGGCGCGCAT CGCGACCGGA GCGCTCACCG CCACGGCCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT