BuviA.00104.a
Beta-lactamase
| CENTER ID: | BuviA.00104.a |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00104.a.B2.GE39146 | Mature protein( BuviA.00104.a ) | 28 | 291 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00104.a.B2.PS02487 | Mature protein( BuviA.00104.a ) | 28 | 291 |
Structures
5HW3
DEPOSITED: 1/29/2016
DETERMINATION: XRay
CLONE: BuviA.00104.a.B2.GE39146
PROTEIN: BuviA.00104.a.B2.PS02487
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_143180 |
| PATRIC ID: | fig|269482.11.peg.3461 |
| RefSeq: | YP_001117160.1 |
| UniProt: | A4JN42 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPHSLLRRKL LIGLPLLAGT CMAPALRAAE ESPLAEIERR SGGRLGVFAI DTGSGRTLGH RADERFLMCS TFKGLLAAQI LARVDSGSER LDRLVHYTEK DLIFTSPVTK ANVAQGAMSI EALCRAVLVE SDNTAAILLM RSAGGPAALT RFVRGLGDTV TRSDRYEPDS NRYHGVLDTT TPKAIAATAQ RLLLGDVLSA GSRARLERGM TDCKPGLNRI RAALPAGWLA ADRPGTSVDR ETNDYALVRP PGRAPLLVAV YYDAPGVSMD AREAVLREAG SAFVQWATNA G
NT Sequence
GTGCCCCACT CGCTCCTGCG CAGAAAACTA CTGATCGGAT TACCGCTGCT CGCCGGCACG TGCATGGCTC CCGCGCTGCG CGCAGCCGAA GAATCGCCGC TTGCCGAAAT CGAGCGCAGA AGCGGCGGCC GCCTCGGCGT GTTCGCGATC GATACCGGCT CGGGACGCAC GCTCGGCCAT CGCGCCGACG AACGTTTCCT GATGTGCAGC ACGTTCAAGG GACTGCTCGC CGCGCAGATC CTGGCACGCG TCGACAGCGG CTCGGAGCGG CTAGATCGCC TCGTCCACTA CACGGAAAAG GACCTGATTT TCACGTCGCC GGTGACGAAG GCCAACGTCG CTCAGGGTGC GATGTCGATC GAGGCGCTCT GCCGCGCGGT TTTGGTGGAG AGCGACAACA CGGCCGCGAT CCTGCTGATG CGCAGCGCCG GCGGCCCTGC GGCGCTGACG CGCTTCGTTC GCGGCCTCGG CGATACCGTG ACGCGCTCCG ACCGCTACGA GCCCGACTCG AACCGCTATC ACGGCGTGCT CGACACCACC ACGCCGAAGG CCATCGCCGC CACGGCGCAG CGCCTGCTGC TCGGCGACGT GCTCAGCGCC GGCTCGCGCG CGCGACTGGA GCGCGGCATG ACCGACTGCA AGCCGGGGCT CAACCGGATT CGCGCCGCGT TGCCCGCCGG TTGGCTAGCC GCCGACCGGC CCGGCACCAG CGTCGACCGC GAGACGAACG ACTACGCGCT AGTCCGTCCG CCCGGGCGCG CGCCGTTGCT CGTCGCCGTC TACTACGACG CGCCGGGTGT CAGCATGGAC GCACGCGAGG CCGTGTTGCG CGAGGCCGGC TCGGCATTCG TGCAGTGGGC GACGAACGCG GGA
Details for BuviA.00104.a.B2.GE39146
| HARVESTED ON: | 5/6/2015 |
| SEQUENCED ON: | 5/6/2015 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA AEESPLAEIE RRSGGRLGVF AIDTGSGRTL GHRADERFLM CSTFKGLLAA QILARVDSGS ERLDRLVHYT EKDLIFTSPV TKANVAQGAM SIEALCRAVL VESDNTAAIL LMRSAGGPAA LTRFVRGLGD TVTRSDRYEP DSNRYHGVLD TTTPKAIAAT AQRLLLGDVL SAGSRARLER GMTDCKPGLN RIRAALPAGW LAADRPGTSV DRETNDYALV RPPGRAPLLV AVYYDAPGVS MDAREAVLRE AGSAFVQWAT NAG
Validated NT Sequence
atcttactca cttatcccgc gttcgtcgcc cactgcacga atgccgagcc ggcctcgcgc aacacggcct cgcgtgcgtc catgctgaca cccggcgcgt cgtagtagac ggcgacgagc aacggcgcgc gcccgggcgg acggactagc gcgtagtcgt tcgtctcgcg gtcgacgctg gtgccgggcc ggtcggcggc tagccaaccg gcgggcaacg cggcgcgaat ccggttgagc cccggcttgc agtcggtcat gccgcgctcc agtcgcgcgc gcgagccggc gctgagcacg tcgccgagca gcaggcgctg cgccgtggcg gcgatggcct tcggcgtggt ggtgtcgagc acgccgtgat agcggttcga gtcgggctcg tagcggtcgg agcgcgtcac ggtatcgccg aggccgcgaa cgaagcgcgt cagcgccgca gggccgccgg cgctgcgcat cagcaggatc gcggccgtgt tgtcgctctc caccaaaacc gcgcggcaga gcgcctcgat cgacatcgca ccctgagcga cgttggcctt cgtcaccggc gacgtgaaaa tcaggtcctt ttccgtgtag tggacgaggc gatctagccg ctccgagccg ctgtcgacgc gtgccaggat ctgcgcggcg agcagtccct tgaacgtgct gcacatcagg aaacgttcgt cggcgcgatg gccgagcgtg cgtcccgagc cggtatcgat cgcgaacacg ccgaggcggc cgccgcttct gcgctcgatt tcggcaagcg gcgattcttc ggctgccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHAA EESPLAEIER RSGGRLGVFA IDTGSGRTLG HRADERFLMC STFKGLLAAQ ILARVDSGSE RLDRLVHYTE KDLIFTSPVT KANVAQGAMS IEALCRAVLV ESDNTAAILL MRSAGGPAAL TRFVRGLGDT VTRSDRYEPD SNRYHGVLDT TTPKAIAATA QRLLLGDVLS AGSRARLERG MTDCKPGLNR IRAALPAGWL AADRPGTSVD RETNDYALVR PPGRAPLLVA VYYDAPGVSM DAREAVLREA GSAFVQWATN AG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcagccg aagaatcgcc gcttgccgaa atcgagcgca gaagcggcgg ccgcctcggc gtgttcgcga tcgataccgg ctcgggacgc acgctcggcc atcgcgccga cgaacgtttc ctgatgtgca gcacgttcaa gggactgctc gccgcgcaga tcctggcacg cgtcgacagc ggctcggagc ggctagatcg cctcgtccac tacacggaaa aggacctgat tttcacgtcg ccggtgacga aggccaacgt cgctcagggt gcgatgtcga tcgaggcgct ctgccgcgcg gttttggtgg agagcgacaa cacggccgcg atcctgctga tgcgcagcgc cggcggccct gcggcgctga cgcgcttcgt tcgcggcctc ggcgataccg tgacgcgctc cgaccgctac gagcccgact cgaaccgcta tcacggcgtg ctcgacacca ccacgccgaa ggccatcgcc gccacggcgc agcgcctgct gctcggcgac gtgctcagcg ccggctcgcg cgcgcgactg gagcgcggca tgaccgactg caagccgggg ctcaaccgga ttcgcgccgc gttgcccgcc ggttggctag ccgccgaccg gcccggcacc agcgtcgacc gcgagacgaa cgactacgcg ctagtccgtc cgcccgggcg cgcgccgttg ctcgtcgccg tctactacga cgcgccgggt gtcagcatgg acgcacgcga ggccgtgttg cgcgaggccg gctcggcatt cgtgcagtgg gcgacgaacg cgggatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuviA.00104.a.B2.PS02487
| PURIFICATION DATe: | 9/2/2015 |
| CONCENTRATION: | 55.63mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA AEESPLAEIE RRSGGRLGVF AIDTGSGRTL GHRADERFLM CSTFKGLLAA QILARVDSGS ERLDRLVHYT EKDLIFTSPV TKANVAQGAM SIEALCRAVL VESDNTAAIL LMRSAGGPAA LTRFVRGLGD TVTRSDRYEP DSNRYHGVLD TTTPKAIAAT AQRLLLGDVL SAGSRARLER GMTDCKPGLN RIRAALPAGW LAADRPGTSV DRETNDYALV RPPGRAPLLV AVYYDAPGVS MDAREAVLRE AGSAFVQWAT NAG
Validated NT Sequence
atcttactca cttatcccgc gttcgtcgcc cactgcacga atgccgagcc ggcctcgcgc aacacggcct cgcgtgcgtc catgctgaca cccggcgcgt cgtagtagac ggcgacgagc aacggcgcgc gcccgggcgg acggactagc gcgtagtcgt tcgtctcgcg gtcgacgctg gtgccgggcc ggtcggcggc tagccaaccg gcgggcaacg cggcgcgaat ccggttgagc cccggcttgc agtcggtcat gccgcgctcc agtcgcgcgc gcgagccggc gctgagcacg tcgccgagca gcaggcgctg cgccgtggcg gcgatggcct tcggcgtggt ggtgtcgagc acgccgtgat agcggttcga gtcgggctcg tagcggtcgg agcgcgtcac ggtatcgccg aggccgcgaa cgaagcgcgt cagcgccgca gggccgccgg cgctgcgcat cagcaggatc gcggccgtgt tgtcgctctc caccaaaacc gcgcggcaga gcgcctcgat cgacatcgca ccctgagcga cgttggcctt cgtcaccggc gacgtgaaaa tcaggtcctt ttccgtgtag tggacgaggc gatctagccg ctccgagccg ctgtcgacgc gtgccaggat ctgcgcggcg agcagtccct tgaacgtgct gcacatcagg aaacgttcgt cggcgcgatg gccgagcgtg cgtcccgagc cggtatcgat cgcgaacacg ccgaggcggc cgccgcttct gcgctcgatt tcggcaagcg gcgattcttc ggctgccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHAA EESPLAEIER RSGGRLGVFA IDTGSGRTLG HRADERFLMC STFKGLLAAQ ILARVDSGSE RLDRLVHYTE KDLIFTSPVT KANVAQGAMS IEALCRAVLV ESDNTAAILL MRSAGGPAAL TRFVRGLGDT VTRSDRYEPD SNRYHGVLDT TTPKAIAATA QRLLLGDVLS AGSRARLERG MTDCKPGLNR IRAALPAGWL AADRPGTSVD RETNDYALVR PPGRAPLLVA VYYDAPGVSM DAREAVLREA GSAFVQWATN AG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGCCG AAGAATCGCC GCTTGCCGAA ATCGAGCGCA GAAGCGGCGG CCGCCTCGGC GTGTTCGCGA TCGATACCGG CTCGGGACGC ACGCTCGGCC ATCGCGCCGA CGAACGTTTC CTGATGTGCA GCACGTTCAA GGGACTGCTC GCCGCGCAGA TCCTGGCACG CGTCGACAGC GGCTCGGAGC GGCTAGATCG CCTCGTCCAC TACACGGAAA AGGACCTGAT TTTCACGTCG CCGGTGACGA AGGCCAACGT CGCTCAGGGT GCGATGTCGA TCGAGGCGCT CTGCCGCGCG GTTTTGGTGG AGAGCGACAA CACGGCCGCG ATCCTGCTGA TGCGCAGCGC CGGCGGCCCT GCGGCGCTGA CGCGCTTCGT TCGCGGCCTC GGCGATACCG TGACGCGCTC CGACCGCTAC GAGCCCGACT CGAACCGCTA TCACGGCGTG CTCGACACCA CCACGCCGAA GGCCATCGCC GCCACGGCGC AGCGCCTGCT GCTCGGCGAC GTGCTCAGCG CCGGCTCGCG CGCGCGACTG GAGCGCGGCA TGACCGACTG CAAGCCGGGG CTCAACCGGA TTCGCGCCGC GTTGCCCGCC GGTTGGCTAG CCGCCGACCG GCCCGGCACC AGCGTCGACC GCGAGACGAA CGACTACGCG CTAGTCCGTC CGCCCGGGCG CGCGCCGTTG CTCGTCGCCG TCTACTACGA CGCGCCGGGT GTCAGCATGG ACGCACGCGA GGCCGTGTTG CGCGAGGCCG GCTCGGCATT CGTGCAGTGG GCGACGAACG CGGGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT