SSGCID: Target Page

BuviA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID:	BuviA.00102.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00102.a.A1.GE32424	Full length( BuviA.00102.a )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6421
RefSeq:	YP_001120024.1
UniProt:	A4JFY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLCDFEVGL DQPFFLIAGT CVVESEQMTI DTAGRLKEIC AKLNVPFIYK SSYDKANRSS GKSFRGLGMD EGLRILGEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH PNPAEAKSDG PNAVPLHRMG ALLETLVTLD QAVKRNPLLE NDFN

NT Sequence

ATGAAGCTGT GCGATTTCGA GGTCGGCCTC GACCAGCCCT TCTTCCTGAT CGCGGGTACC TGCGTCGTCG AATCGGAGCA GATGACGATC GACACCGCGG GCCGCCTGAA GGAAATCTGC GCGAAGCTGA ACGTTCCGTT CATCTACAAG TCGTCCTACG ACAAGGCGAA CCGCAGCTCG GGCAAGTCGT TTCGCGGCCT CGGAATGGAC GAGGGGCTGC GCATCCTCGG CGAGGTGAAG CGCCAGCTCG GCCTGCCGGT GCTGACCGAC GTGCATTCGA TCGACGAGAT CGAGCAGGTC GCGTCGGTCG TCGACGTGCT GCAGACGCCC GCGTTCCTGT GCCGCCAGAC CGACTTCATC CACGCGTGCG CGCGCTCGGG CAAGCCGGTC AACATCAAGA AAGGCCAGTT CCTCGCGCCG CACGACATGA AGAACGTGAT CGACAAGGCG CGCGACGCCG CACGCGAAGC CGGTCTGTCG GAAGACCGCT TCATGGCGTG CGAGCGCGGC GTCTCGTTCG GCTACAACAA CCTCGTGTCC GACATGCGCT CGCTCGCGAT CATGCGCGAA ACGAATGCGC CGGTCGTGTT CGACGCGACG CACTCGGTGC AGCTGCCGGG CGGGCAGGGC ACGAGTTCGG GCGGTCAGCG CGAATTCGTG CCGGTGCTCG CGCGCGCCGC GGTCGCGACG GGTGTCGCCG GCCTGTTCAT GGAAACGCAT CCGAATCCGG CCGAAGCGAA GTCCGACGGC CCGAACGCGG TGCCGCTGCA CCGGATGGGC GCGCTGCTCG AGACGCTCGT CACGCTCGAC CAGGCGGTGA AGCGTAATCC GCTGCTGGAA AACGATTTCA AC

Details for BuviA.00102.a.A1.GE32424

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKLCDFEVG LDQPFFLIAG TCVVESEQMT IDTAGRLKEI CAKLNVPFIY KSSYDKANRS SGKSFRGLGM DEGLRILGEV KRQLGLPVLT DVHSIDEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK ARDAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETNAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA TGVAGLFMET HPNPAEAKSD XPNAVPLHRM GALLETLVTL DQAVKRNPLL ENDFNX

Validated NT Sequence

ttttntttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaagc tgtgcgattt cgaggtcggc ctcgaccagc ccttcttcct gatcgcgggt acctgcgtcg tcgaatcgga gcagatgacg atcgacaccg cgggccgcct gaaggaaatc tgcgcgaagc tgaacgttcc gttcatctac aagtcgtcct acgacaaggc gaaccgcagc tcgggcaagt cgtttcgcgg cctcggaatg gacgaggggc tgcgcatcct cggcgaggtg aagcgccagc tcggcctgcc ggtgctgacc gacgtgcatt cgatcgacga gatcgagcag gtcgcgtcgg tcgtcgacgt gctgcagacg cccgcgttcc tgtgccgcca gaccgacttc atccacgcgt gcgcgcgctc gggcaagccg gtcaacatca agaaaggcca gttcctcgcg ccgcacgaca tgaagaacgt gatcgacaag gcgcgcgacg ccgcacgcga agccggtctg tcggaagacc gcttcatggc gtgcgagcgc ggcgtctcgt tcggctacaa caacctcgtg tccgacatgc gctcgctcgc gatcatgcgc gaaacgaatg cgccggtcgt gttcgacgcg acgcactcgg tgcagctgcc gggcgggcag ggcacgagtt cgggcggtca gcgcgaattc gtgccggtgc tcgcgcgcgc cgcggtcgcg acgggtgtcg ccggcctgtt catggaaacg catccgaatc cggccgaagc gaagtccgac ngcccgaacg cggtgccgct gcaccggatg ggcgcgctgc tcgagacgct cgtcacgctc gaccaggcgg tgaagcgtaa tccgctgctg gaaaacgatt tcaactanta aacagcacga acnagttctg cagccaagct tctcnaggan cnnnctgcta acaaagcccg aaangaagct gantngtgct gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctgtgc gatttcgagg tcggcctcga ccagcccttc ttcctgatcg cgggtacctg cgtcgtcgaa tcggagcaga tgacgatcga caccgcgggc cgcctgaagg aaatctgcgc gaagctgaac gttccgttca tctacaagtc gtcctacgac aaggcgaacc gcagctcggg caagtcgttt cgcggcctcg gaatggacga ggggctgcgc atcctcggcg aggtgaagcg ccagctcggc ctgccggtgc tgaccgacgt gcattcgatc gacgagatcg agcaggtcgc gtcggtcgtc gacgtgctgc agacgcccgc gttcctgtgc cgccagaccg acttcatcca cgcgtgcgcg cgctcgggca agccggtcaa catcaagaaa ggccagttcc tcgcgccgca cgacatgaag aacgtgatcg acaaggcgcg cgacgccgca cgcgaagccg gtctgtcgga agaccgcttc atggcgtgcg agcgcggcgt ctcgttcggc tacaacaacc tcgtgtccga catgcgctcg ctcgcgatca tgcgcgaaac gaatgcgccg gtcgtgttcg acgcgacgca ctcggtgcag ctgccgggcg ggcagggcac gagttcgggc ggtcagcgcg aattcgtgcc ggtgctcgcg cgcgccgcgg tcgcgacggg tgtcgccggc ctgttcatgg aaacgcatcc gaatccggcc gaagcgaagt ccgacggccc gaacgcggtg ccgctgcacc ggatgggcgc gctgctcgag acgctcgtca cgctcgacca ggcggtgaag cgtaatccgc tgctggaaaa cgatttcaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa