SSGCID: Target Page

BuviA.00090.b

3-deoxy-D-arabinoheptulosonate-7-phosphate synthase

CENTER ID:	BuviA.00090.b
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00090.b.B1.GE38096	Full length( BuviA.00090.b )		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.2438
RefSeq:	YP_001116176.1
UniProt:	A4JKA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHNLDNPSHD REVGSADATQ DTTRIDDVRI GAVRPLISPA LLQDELPVPA GTQTLVEDTR RAIGDILHGR DDRLLVVVGP CSIHDHDQAL EYARRLKAAA DALQDDLLIT MRVYFEKPRT TVGWKGYIND PRLDGSFRIN EGLRAARQLL LDINALGLPA STEFLDLLSP QYIADLIAWG AIGARTTESQ SHRQLASGLS CPIGFKNGTD GGVQVAADAI VAARASHAFM GMTKMGMAAI FETRGNDDAH VILRGGKNGP NYDAEHVEAS CAVLRKSGLR EQVMVDCSHA NSNKSHERQI DVAQDLARQL SQGEQRIIGV MVESHLEAGR QDLKPGVPLK YGVSITDACL SWSQTEPVLD VLAQAVRQRR GASRNA

NT Sequence

ATGCATAACC TCGACAATCC CTCCCACGAT CGCGAGGTAG GCAGCGCCGA CGCGACGCAA GACACGACCC GCATCGACGA CGTCCGCATC GGCGCCGTGC GCCCGCTGAT CTCGCCCGCG CTGCTGCAGG ACGAGCTGCC GGTGCCGGCC GGCACGCAGA CGCTCGTCGA GGACACGCGG CGCGCGATCG GCGACATCCT GCACGGCCGC GACGACCGGC TGCTGGTCGT CGTCGGCCCG TGCTCGATCC ACGACCACGA CCAGGCGCTC GAGTACGCGC GCCGCCTGAA GGCCGCCGCC GACGCGCTGC AGGACGATCT GCTGATCACG ATGCGCGTGT ACTTCGAGAA GCCGCGCACG ACGGTCGGCT GGAAGGGCTA CATCAACGAT CCGCGTCTGG ACGGCAGCTT CCGCATCAAC GAAGGGCTGC GCGCGGCACG ACAGCTGCTG CTCGACATCA ACGCGCTCGG CCTGCCGGCG TCGACCGAAT TCCTCGACCT GCTGAGCCCG CAGTACATCG CGGATCTGAT CGCGTGGGGC GCCATCGGCG CGCGCACCAC CGAGAGCCAG AGCCATCGTC AGCTTGCATC GGGCCTGAGC TGCCCGATCG GCTTCAAGAA CGGTACCGAC GGCGGCGTGC AGGTCGCGGC CGATGCGATC GTCGCCGCGC GCGCGAGCCA CGCGTTCATG GGAATGACGA AGATGGGCAT GGCCGCGATC TTCGAGACGC GCGGCAACGA CGACGCGCAC GTGATCCTGC GCGGCGGCAA GAATGGTCCG AACTACGACG CCGAGCACGT CGAAGCGAGC TGCGCGGTGC TGCGCAAGAG CGGGTTGCGC GAGCAGGTGA TGGTCGATTG CTCGCATGCG AATTCGAACA AGTCGCACGA GCGGCAGATC GACGTCGCGC AGGATCTCGC GCGCCAGCTG TCGCAGGGCG AGCAGCGCAT CATCGGCGTG ATGGTGGAGA GCCATCTCGA GGCCGGCCGT CAGGATCTGA AGCCGGGCGT GCCGCTGAAG TACGGCGTGT CGATCACCGA TGCATGTCTG AGCTGGTCGC AGACGGAGCC GGTGCTCGAC GTGCTCGCGC AAGCCGTGCG GCAGCGCCGC GGCGCGTCGC GCAACGCG

Details for BuviA.00090.b.B1.GE38096

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	88

Validated AA Sequence

MAHHHHHHMH NLDNPSHDRE VGSADATQDT TRIDDVRIGA VRPLISPALL QDELPVPAGT QTLVEDTRRA IGDILHGRDD RLLVVVGPCS IHDHDQALEY ARRLKAAADA LQDDLLITMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRAARQLLLD INALGLPAST EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQVAADAIVA ARASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKNGPNY DAEHVEASCA VLRKSGLREQ VMVDCSHANS NKSHERQIDV AQDLRAQLSQ GEQRIIGXMV XSHSRGRPSG X

Validated NT Sequence

tntnaanttt nnnnnaaagg agatatacca tggctcacca ccaccaccac catatgcata acctcgacaa tccctcccac gatcgcgagg taggcagcgc cgacgcgacg caagacacga cccgcatcga cgacgtccgc atcggcgccg tgcgcccgct gatctcgccc gcgctgctgc aggacgagct gccggtgccg gccggcacgc agacgctcgt cgaggacacg cggcgcgcga tcggcgacat cctgcacggc cgcgacgacc ggctgctggt cgtcgtcggc ccgtgctcga tccacgacca cgaccaggcg ctcgagtacg cgcgccgcct gaaggccgcc gccgacgcgc tgcaggacga tctgctgatc acgatgcgcg tgtacttcga gaagccgcgc acgacggtcg gctggaaggg ctacatcaac gatccgcgtc tggacggcag cttccgcatc aacgaagggc tgcgcgcggc acgacagctg ctgctcgaca tcaacgcgct cggcctgccg gcgtcgaccg aattcctcga cctgctgagc ccgcagtaca tcgcggatct gatcgcgtgg ggcgccatcg gcgcgcgcac caccgagagc cagagccatc gtcagcttgc atcgggcctg agctgcccga tcggcttcaa gaacggtacc gacggcggcg tgcaggtcgc ggccgatgcg atcgtcgccg cgcgcgcgag ccacgcgttc atgggaatga cgaagatggg catggccgcg atcttcgaga cgcgcggcaa cgacgacgcg cacgtgatcc tgcgcggcgg caagaatggt ccgaactacg acgccgagca cgtcgaagcg agctgcgcgg tgctgcgcaa gagcgggttg cgcgagcagg tgatggtcga ttgctcgcat gcgaattcga acaagtcgca cgagcggcag atcgacgtcg cgcaggatct tcgcgcgcag ctgtcgcaag gcgagcagcg catcatcggc ntgatggtgg anagccattc tcgaggccgg ccgtcaggan cctgaagccn gcntgcnctg aagtacggcg nngtcgatca cgatncatgt ctgannggtc nnnnngganc cgntnntnna cntgntcgnn cagccnnncg gcnnnccnng nnnntcccnc anncnnnann naataaaatn ggaccgntgc tnnannnncc nanngna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata acctcgacaa tccctcccac gatcgcgagg taggcagcgc cgacgcgacg caagacacga cccgcatcga cgacgtccgc atcggcgccg tgcgcccgct gatctcgccc gcgctgctgc aggacgagct gccggtgccg gccggcacgc agacgctcgt cgaggacacg cggcgcgcga tcggcgacat cctgcacggc cgcgacgacc ggctgctggt cgtcgtcggc ccgtgctcga tccacgacca cgaccaggcg ctcgagtacg cgcgccgcct gaaggccgcc gccgacgcgc tgcaggacga tctgctgatc acgatgcgcg tgtacttcga gaagccgcgc acgacggtcg gctggaaggg ctacatcaac gatccgcgtc tggacggcag cttccgcatc aacgaagggc tgcgcgcggc acgacagctg ctgctcgaca tcaacgcgct cggcctgccg gcgtcgaccg aattcctcga cctgctgagc ccgcagtaca tcgcggatct gatcgcgtgg ggcgccatcg gcgcgcgcac caccgagagc cagagccatc gtcagcttgc atcgggcctg agctgcccga tcggcttcaa gaacggtacc gacggcggcg tgcaggtcgc ggccgatgcg atcgtcgccg cgcgcgcgag ccacgcgttc atgggaatga cgaagatggg catggccgcg atcttcgaga cgcgcggcaa cgacgacgcg cacgtgatcc tgcgcggcgg caagaatggt ccgaactacg acgccgagca cgtcgaagcg agctgcgcgg tgctgcgcaa gagcgggttg cgcgagcagg tgatggtcga ttgctcgcat gcgaattcga acaagtcgca cgagcggcag atcgacgtcg cgcaggatct cgcgcgccag ctgtcgcagg gcgagcagcg catcatcggc gtgatggtgg agagccatct cgaggccggc cgtcaggatc tgaagccggg cgtgccgctg aagtacggcg tgtcgatcac cgatgcatgt ctgagctggt cgcagacgga gccggtgctc gacgtgctcg cgcaagccgt gcggcagcgc cgcggcgcgt cgcgcaacgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat