SSGCID: Target Page

BuviA.00090.a

3-deoxy-D-arabinoheptulosonate-7-phosphate synthase

CENTER ID:	BuviA.00090.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00090.a.B1.GE38095	Full length( BuviA.00090.a )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.4908
RefSeq:	YP_001118546.1
UniProt:	A4JBQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPPHNTDDVR IRELKELTPP AHLIREFACS EAASELIYES RRSMHRILHG MDDRLIVVIG PCSIHDTKAA LEYAGRLVKE RERFKGELEI VMRVYFEKPR TTVGWKGLIN DPHLDNSFKI NEGLRTAREL LLQINEMGLP AATEYLDMIS PQYIADLISW GAIGARTTES QVHRELASGL SCPVGFKNGT DGNVKIAVDA IKAASQPHHF LSVTKGGHSA IVSTAGNEDC HVILRGGKTP NYDADSVNAA CADIGKAGLA ARLMIDASHA NSSKKHENQI PVCADIGRQV AAGDERIVGV MIESHLVEGR QDLKEGCALT YGQSITDACI AWDDSVKVLE GLAEAVKARR VARGSGN

NT Sequence

ATGCCCCCGC ACAACACCGA CGACGTCCGC ATCCGCGAAC TCAAGGAACT GACGCCGCCC GCCCACCTGA TCCGCGAATT CGCATGCTCC GAGGCCGCGT CCGAGCTGAT CTACGAGTCG CGCCGTTCGA TGCACCGGAT CCTGCACGGG ATGGACGACC GGCTGATCGT CGTGATCGGG CCGTGCTCGA TCCACGACAC GAAGGCGGCG CTCGAATATG CGGGGCGGCT CGTGAAGGAG CGGGAGCGCT TCAAGGGCGA GCTGGAAATC GTGATGCGCG TGTACTTCGA AAAGCCGCGC ACGACGGTCG GCTGGAAGGG GCTGATCAAC GATCCGCATC TCGACAACAG CTTCAAGATC AACGAAGGGC TGCGCACGGC GCGCGAGCTG CTGCTGCAGA TCAACGAGAT GGGGCTGCCG GCCGCGACCG AATACCTCGA CATGATCAGC CCGCAGTACA TCGCCGACCT GATCTCGTGG GGCGCGATCG GCGCGCGCAC GACCGAGTCG CAGGTGCATC GCGAACTCGC GTCGGGGCTG TCGTGCCCGG TCGGCTTCAA GAACGGCACC GACGGCAACG TGAAGATCGC CGTCGACGCG ATCAAGGCCG CGTCGCAGCC GCACCATTTC CTGTCGGTGA CCAAGGGCGG CCATTCGGCG ATCGTGTCGA CGGCCGGCAA CGAAGACTGT CACGTGATCC TGCGCGGCGG CAAGACGCCG AACTACGACG CGGACAGCGT GAACGCCGCC TGCGCGGACA TCGGCAAGGC CGGCCTCGCC GCGCGCCTGA TGATCGACGC GAGCCATGCG AACAGCTCGA AGAAGCACGA GAACCAGATC CCGGTGTGCG CGGACATCGG CCGTCAGGTC GCCGCCGGCG ACGAGCGGAT CGTCGGCGTG ATGATCGAGT CGCACCTCGT CGAGGGGCGC CAGGACCTGA AGGAAGGCTG CGCGCTGACC TACGGCCAGA GCATCACCGA CGCATGCATC GCGTGGGACG ACAGCGTGAA GGTGCTCGAA GGGCTCGCGG AAGCGGTGAA GGCGCGCCGC GTCGCACGCG GCAGCGGCAA C

Details for BuviA.00090.a.B1.GE38095

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYESRR SMHRILHGMD DRLIVVIGPC SIHDTKAALE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAA TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKTPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDERIVGVMI ESXLVEGRQD LKEGCALTYG QSITDACIAW DDSXKVLEXS XKR

Validated NT Sequence

tnnnnnnttt tntttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgcccccg cacaacaccg acgacgtccg catccgcgaa ctcaaggaac tgacgccgcc cgcccatctg atccgcgaat tcgcatgctc cgaggccgcg tccgagctga tctacgagtc gcgccgttcg atgcaccgga tcctgcacgg gatggacgac cggctgatcg tcgtgatcgg gccgtgctcg atccacgaca cgaaggcggc gctcgaatat gcggggcggc tcgtgaagga gcgggagcgc ttcaagggcg agctggaaat cgtgatgcgc gtgtacttcg aaaagccgcg cacgacggtc ggctggaagg ggctgatcaa cgatccgcat ctcgacaaca gcttcaagat caacgaaggg ctgcgcacgg cgcgcgagct gctgctgcag atcaacgaga tggggctgcc ggccgcgacc gaatacctcg acatgatcag cccgcagtac atcgccgacc tgatctcgtg gggcgcgatc ggcgcgcgca cgaccgagtc gcaggtgcat cgcgaactcg cgtcggggct gtcgtgcccg gtcggcttca agaacggcac cgacggcaac gtgaagatcg ccgtcgacgc gatcaaggcc gcgtcgcagc cgcaccattt cctgtcggtg accaagggcg gccattcggc gatcgtgtcg acggccggca acgaagactg tcacgtgatc ctgcgcggcg gcaagacgcc gaactacgac gcggacagcg tgaacgccgc ctgcgcggac atcggcaagg ccggcctcgc cgcgcgcctg atgatcgacg cgagccatgc gaacagctcg aagaagcacg agaaccagat cccggtgtgc gcggacatcg gccgtcaggt cgccgccggc gacgagcgga tcgtcggcgt gatgatcgag tcgcanctcg tcgaggggcg ccaggacctg aaggaaggct gcgcgctgac ctacggccag agcatcaccg acgcatgcat cgcgtgggac gacagcntga aggtgctcga annctcgcng aagcggtgaa ggcncgcncg tcnnacncgg cagcngcact aagtgagtaa gagatccg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc cgcacaacac cgacgacgtc cgcatccgcg aactcaagga actgacgccg cccgcccacc tgatccgcga attcgcatgc tccgaggccg cgtccgagct gatctacgag tcgcgccgtt cgatgcaccg gatcctgcac gggatggacg accggctgat cgtcgtgatc gggccgtgct cgatccacga cacgaaggcg gcgctcgaat atgcggggcg gctcgtgaag gagcgggagc gcttcaaggg cgagctggaa atcgtgatgc gcgtgtactt cgaaaagccg cgcacgacgg tcggctggaa ggggctgatc aacgatccgc atctcgacaa cagcttcaag atcaacgaag ggctgcgcac ggcgcgcgag ctgctgctgc agatcaacga gatggggctg ccggccgcga ccgaatacct cgacatgatc agcccgcagt acatcgccga cctgatctcg tggggcgcga tcggcgcgcg cacgaccgag tcgcaggtgc atcgcgaact cgcgtcgggg ctgtcgtgcc cggtcggctt caagaacggc accgacggca acgtgaagat cgccgtcgac gcgatcaagg ccgcgtcgca gccgcaccat ttcctgtcgg tgaccaaggg cggccattcg gcgatcgtgt cgacggccgg caacgaagac tgtcacgtga tcctgcgcgg cggcaagacg ccgaactacg acgcggacag cgtgaacgcc gcctgcgcgg acatcggcaa ggccggcctc gccgcgcgcc tgatgatcga cgcgagccat gcgaacagct cgaagaagca cgagaaccag atcccggtgt gcgcggacat cggccgtcag gtcgccgccg gcgacgagcg gatcgtcggc gtgatgatcg agtcgcacct cgtcgagggg cgccaggacc tgaaggaagg ctgcgcgctg acctacggcc agagcatcac cgacgcatgc atcgcgtggg acgacagcgt gaaggtgctc gaagggctcg cggaagcggt gaaggcgcgc cgcgtcgcac gcggcagcgg caactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat