BuviA.00088.a

Ornithine carbamoyltransferase

CENTER ID: BuviA.00088.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00088.a.A1.GE32423 Full length( BuviA.00088.a ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00088.a.A1.PS01329 Full length( BuviA.00088.a ) 1 309
BuviA.00088.a.A1.PS01330 Full length( BuviA.00088.a ) 1 309

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.6902
RefSeq: YP_001120473.1
UniProt: A4JH85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDVITRFAQN SRVPVINGLT NEYHPCQVLA DIFTYYEHRG PIAGKTVAWV GDANNMLYTW IEAAQILGFK LRLSTPPGYA LDMKLVSPDS APFYEVFDDP NEACKGADLV TTDVWTSMGF EAENEARKQA FADWCVDEEM MGYANADALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRLKH
NT Sequence
ATGACCGCCA AAACCATTCG TCACTACCTG CAGTTCAAGG ATTTCTCGCT GGAAGACTAC GAGTACGTGC TCGAACGCAC GGGTATCCTG AAGCGCAAGT TCAAGAACTA CGAGACCTAC CATCCGCTGC ACGATCGCAC GCTCGCGATG ATCTTCGAGA AGAGCTCGAC GCGCACGCGG CTGTCGTTCG AAGCCGGCAT CTTCCAGCTC GGCGGCCACG CGGTGTTCAT GAGCACGCGC GACACGCAGC TCGGCCGCGG CGAGCCGGTC GAGGATTCCG CGCAGGTGAT CTCGCGGATG GTCGACATCA TCATGATCCG CACGTTCGAG CAGGACGTGA TCACGCGCTT CGCGCAAAAC TCGCGCGTGC CGGTGATCAA CGGCCTGACC AACGAATACC ATCCGTGTCA GGTCCTCGCC GACATCTTCA CGTACTACGA ACACCGCGGC CCGATCGCCG GCAAGACGGT CGCGTGGGTC GGCGATGCGA ACAACATGCT GTACACGTGG ATCGAAGCCG CGCAGATTCT CGGCTTCAAG CTGCGCCTGT CGACGCCGCC GGGCTATGCG CTCGACATGA AGCTCGTGTC GCCCGACAGC GCGCCGTTCT ACGAAGTGTT CGACGATCCG AACGAAGCGT GCAAGGGCGC CGATCTCGTG ACGACCGACG TGTGGACGAG CATGGGCTTC GAAGCGGAGA ACGAAGCGCG CAAGCAGGCG TTCGCCGACT GGTGCGTCGA CGAGGAAATG ATGGGCTATG CGAACGCGGA CGCGCTGTTC ATGCACTGCC TGCCCGCGCA CCGCGGCGAG GAAGTGACGG CCGGCGTGAT CGACGGGCCG CAGAGCGTGG TGTGGGACGA GGCGGAAAAC CGCCTGCACG TGCAGAAGGC GCTGATGGAG TTCCTGCTGC TGGGCCGCCT CAAGCAC
Details for BuviA.00088.a.A1.GE32423
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGYANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRL
Validated NT Sequence
tgaggcggcc cagcagcagg aactccatca gcgccttctg cacgtgcagg cggttttccg cctcgtccca caccacgctc tgcggcccgt cgatcacgcc ggccgtcact tcctcgccgc ggtgcgcggg caggcagtgc atgaacagcg cgtccgcgtt cgcatagccc atcatttcct cgtcgacgca ccagtcggcg aacgcctgct tgcgcgcttc gttctccgct tcgaagccca tgctcgtcca cacgtcggtc gtcacgagat cggcgccctt gcacgcttcg ttcggatcgt cgaacacttc gtagaacggc gcgctgtcgg gcgacacgag cttcatgtcg agcgcatagc ccggcggcgt cgacaggcgc agcttgaagc cgagaatctg cgcggcttcg atccacgtgt acagcatgtt gttcgcatcg ccgacccacg cgaccgtctt gccggcgatc gggccgcggt gttcgtagta cgtgaagatg tcggcgagga cctgacacgg atggtattcg ttggtcaggc cgttgatcac cggcacgcgc gagttttgcg cgaagcgcgt gatcacgtcc tgctcgaacg tgcggatcat gatgatgtcg accatccgcg agatcacctg cgcggaatcc tcgaccggct cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac cgcgtggccg ccgagctgga agatgccggc ttcgaacgac agccgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcgatc gtgcagcgga tggtaggtct cgtagttctt gaacttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGYANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLKH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgccaaa accattcgtc actacctgca gttcaaggat ttctcgctgg aagactacga gtacgtgctc gaacgcacgg gtatcctgaa gcgcaagttc aagaactacg agacctacca tccgctgcac gatcgcacgc tcgcgatgat cttcgagaag agctcgacgc gcacgcggct gtcgttcgaa gccggcatct tccagctcgg cggccacgcg gtgttcatga gcacgcgcga cacgcagctc ggccgcggcg agccggtcga ggattccgcg caggtgatct cgcggatggt cgacatcatc atgatccgca cgttcgagca ggacgtgatc acgcgcttcg cgcaaaactc gcgcgtgccg gtgatcaacg gcctgaccaa cgaataccat ccgtgtcagg tcctcgccga catcttcacg tactacgaac accgcggccc gatcgccggc aagacggtcg cgtgggtcgg cgatgcgaac aacatgctgt acacgtggat cgaagccgcg cagattctcg gcttcaagct gcgcctgtcg acgccgccgg gctatgcgct cgacatgaag ctcgtgtcgc ccgacagcgc gccgttctac gaagtgttcg acgatccgaa cgaagcgtgc aagggcgccg atctcgtgac gaccgacgtg tggacgagca tgggcttcga agcggagaac gaagcgcgca agcaggcgtt cgccgactgg tgcgtcgacg aggaaatgat gggctatgcg aacgcggacg cgctgttcat gcactgcctg cccgcgcacc gcggcgagga agtgacggcc ggcgtgatcg acgggccgca gagcgtggtg tgggacgagg cggaaaaccg cctgcacgtg cagaaggcgc tgatggagtt cctgctgctg ggccgcctca agcactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuviA.00088.a.A1.PS01329
PURIFICATION DATe: 1/6/2012
CONCENTRATION: 81.02mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGYANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRL
Validated NT Sequence
tgaggcggcc cagcagcagg aactccatca gcgccttctg cacgtgcagg cggttttccg cctcgtccca caccacgctc tgcggcccgt cgatcacgcc ggccgtcact tcctcgccgc ggtgcgcggg caggcagtgc atgaacagcg cgtccgcgtt cgcatagccc atcatttcct cgtcgacgca ccagtcggcg aacgcctgct tgcgcgcttc gttctccgct tcgaagccca tgctcgtcca cacgtcggtc gtcacgagat cggcgccctt gcacgcttcg ttcggatcgt cgaacacttc gtagaacggc gcgctgtcgg gcgacacgag cttcatgtcg agcgcatagc ccggcggcgt cgacaggcgc agcttgaagc cgagaatctg cgcggcttcg atccacgtgt acagcatgtt gttcgcatcg ccgacccacg cgaccgtctt gccggcgatc gggccgcggt gttcgtagta cgtgaagatg tcggcgagga cctgacacgg atggtattcg ttggtcaggc cgttgatcac cggcacgcgc gagttttgcg cgaagcgcgt gatcacgtcc tgctcgaacg tgcggatcat gatgatgtcg accatccgcg agatcacctg cgcggaatcc tcgaccggct cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac cgcgtggccg ccgagctgga agatgccggc ttcgaacgac agccgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcgatc gtgcagcgga tggtaggtct cgtagttctt gaacttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGYANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLKH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCCAAA ACCATTCGTC ACTACCTGCA GTTCAAGGAT TTCTCGCTGG AAGACTACGA GTACGTGCTC GAACGCACGG GTATCCTGAA GCGCAAGTTC AAGAACTACG AGACCTACCA TCCGCTGCAC GATCGCACGC TCGCGATGAT CTTCGAGAAG AGCTCGACGC GCACGCGGCT GTCGTTCGAA GCCGGCATCT TCCAGCTCGG CGGCCACGCG GTGTTCATGA GCACGCGCGA CACGCAGCTC GGCCGCGGCG AGCCGGTCGA GGATTCCGCG CAGGTGATCT CGCGGATGGT CGACATCATC ATGATCCGCA CGTTCGAGCA GGACGTGATC ACGCGCTTCG CGCAAAACTC GCGCGTGCCG GTGATCAACG GCCTGACCAA CGAATACCAT CCGTGTCAGG TCCTCGCCGA CATCTTCACG TACTACGAAC ACCGCGGCCC GATCGCCGGC AAGACGGTCG CGTGGGTCGG CGATGCGAAC AACATGCTGT ACACGTGGAT CGAAGCCGCG CAGATTCTCG GCTTCAAGCT GCGCCTGTCG ACGCCGCCGG GCTATGCGCT CGACATGAAG CTCGTGTCGC CCGACAGCGC GCCGTTCTAC GAAGTGTTCG ACGATCCGAA CGAAGCGTGC AAGGGCGCCG ATCTCGTGAC GACCGACGTG TGGACGAGCA TGGGCTTCGA AGCGGAGAAC GAAGCGCGCA AGCAGGCGTT CGCCGACTGG TGCGTCGACG AGGAAATGAT GGGCTATGCG AACGCGGACG CGCTGTTCAT GCACTGCCTG CCCGCGCACC GCGGCGAGGA AGTGACGGCC GGCGTGATCG ACGGGCCGCA GAGCGTGGTG TGGGACGAGG CGGAAAACCG CCTGCACGTG CAGAAGGCGC TGATGGAGTT CCTGCTGCTG GGCCGCCTCA AGCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BuviA.00088.a.A1.PS01330
PURIFICATION DATe: 1/6/2012
CONCENTRATION: 78.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGYANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRL
Validated NT Sequence
tgaggcggcc cagcagcagg aactccatca gcgccttctg cacgtgcagg cggttttccg cctcgtccca caccacgctc tgcggcccgt cgatcacgcc ggccgtcact tcctcgccgc ggtgcgcggg caggcagtgc atgaacagcg cgtccgcgtt cgcatagccc atcatttcct cgtcgacgca ccagtcggcg aacgcctgct tgcgcgcttc gttctccgct tcgaagccca tgctcgtcca cacgtcggtc gtcacgagat cggcgccctt gcacgcttcg ttcggatcgt cgaacacttc gtagaacggc gcgctgtcgg gcgacacgag cttcatgtcg agcgcatagc ccggcggcgt cgacaggcgc agcttgaagc cgagaatctg cgcggcttcg atccacgtgt acagcatgtt gttcgcatcg ccgacccacg cgaccgtctt gccggcgatc gggccgcggt gttcgtagta cgtgaagatg tcggcgagga cctgacacgg atggtattcg ttggtcaggc cgttgatcac cggcacgcgc gagttttgcg cgaagcgcgt gatcacgtcc tgctcgaacg tgcggatcat gatgatgtcg accatccgcg agatcacctg cgcggaatcc tcgaccggct cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac cgcgtggccg ccgagctgga agatgccggc ttcgaacgac agccgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcgatc gtgcagcgga tggtaggtct cgtagttctt gaacttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNEYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGYANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLKH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCCAAA ACCATTCGTC ACTACCTGCA GTTCAAGGAT TTCTCGCTGG AAGACTACGA GTACGTGCTC GAACGCACGG GTATCCTGAA GCGCAAGTTC AAGAACTACG AGACCTACCA TCCGCTGCAC GATCGCACGC TCGCGATGAT CTTCGAGAAG AGCTCGACGC GCACGCGGCT GTCGTTCGAA GCCGGCATCT TCCAGCTCGG CGGCCACGCG GTGTTCATGA GCACGCGCGA CACGCAGCTC GGCCGCGGCG AGCCGGTCGA GGATTCCGCG CAGGTGATCT CGCGGATGGT CGACATCATC ATGATCCGCA CGTTCGAGCA GGACGTGATC ACGCGCTTCG CGCAAAACTC GCGCGTGCCG GTGATCAACG GCCTGACCAA CGAATACCAT CCGTGTCAGG TCCTCGCCGA CATCTTCACG TACTACGAAC ACCGCGGCCC GATCGCCGGC AAGACGGTCG CGTGGGTCGG CGATGCGAAC AACATGCTGT ACACGTGGAT CGAAGCCGCG CAGATTCTCG GCTTCAAGCT GCGCCTGTCG ACGCCGCCGG GCTATGCGCT CGACATGAAG CTCGTGTCGC CCGACAGCGC GCCGTTCTAC GAAGTGTTCG ACGATCCGAA CGAAGCGTGC AAGGGCGCCG ATCTCGTGAC GACCGACGTG TGGACGAGCA TGGGCTTCGA AGCGGAGAAC GAAGCGCGCA AGCAGGCGTT CGCCGACTGG TGCGTCGACG AGGAAATGAT GGGCTATGCG AACGCGGACG CGCTGTTCAT GCACTGCCTG CCCGCGCACC GCGGCGAGGA AGTGACGGCC GGCGTGATCG ACGGGCCGCA GAGCGTGGTG TGGGACGAGG CGGAAAACCG CCTGCACGTG CAGAAGGCGC TGATGGAGTT CCTGCTGCTG GGCCGCCTCA AGCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA