BuviA.00081.b

Aminotransferase, class V

CENTER ID: BuviA.00081.b
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00081.b.B1.GE35354 Full length( BuviA.00081.b ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00081.b.B1.PW35978 Full length( BuviA.00081.b ) 1 384

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.1387
RefSeq: YP_001115182.1
UniProt: A4JRM4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIYLDHNATT APAPAAIDAM LAVLTDVWAN ASSQHGAGQQ AKRTLAAARA TIAGALGCKA REVVFTSGAT EANHLAVLGL GASATASAVR RRLVFGATEH AAQLKLAHAL AAQGRPVDFI AVRPDGVLDL DSAARVIGPD VALVSVMAAN NETGVLMPIA DVKALAQAAG ALLHVDATQL VGKLPFDFAA LGADAVSLSA HKLHGPKGIG ALLVRDGVPL AAQFPGSQER HRRGGTENLP AIVGFAAALE RLADVLADAA RIAALRDAFE AGLRAALPGV HVYGASAPRL PGTSCLRVGR LDADTVLQRC EQIGVCASSG SACSSGGSEP SHVLSAMGVP RDEALGAVRF SLGAATTARD IDYLLAALPP LLKPLLAEAA VQPT
NT Sequence
ATGATCTATC TCGACCACAA CGCGACGACG GCGCCCGCGC CGGCGGCGAT CGACGCGATG CTCGCCGTGC TGACCGACGT GTGGGCCAAC GCGTCGTCGC AGCATGGCGC CGGACAGCAG GCGAAGCGCA CGCTGGCCGC CGCGCGCGCG ACGATCGCCG GCGCGCTCGG TTGCAAGGCC AGGGAAGTGG TCTTCACGAG CGGCGCGACC GAGGCGAATC ATCTCGCGGT GCTCGGGCTC GGGGCGTCGG CCACGGCCTC GGCCGTGCGG CGCAGGCTCG TCTTCGGCGC GACGGAGCAT GCGGCGCAGC TGAAGCTCGC GCACGCGCTC GCCGCCCAGG GCCGTCCGGT CGATTTCATC GCGGTGCGGC CGGACGGCGT GCTCGATCTC GACAGCGCCG CGCGCGTGAT CGGCCCGGAC GTCGCGCTGG TGTCGGTGAT GGCGGCGAAC AACGAGACCG GCGTGCTGAT GCCGATCGCC GACGTGAAGG CGCTCGCGCA GGCGGCGGGC GCGCTGCTGC ACGTCGACGC GACGCAGCTG GTCGGCAAGC TGCCGTTCGA TTTCGCGGCG CTCGGCGCCG ACGCGGTGTC GCTGTCCGCG CACAAGCTGC ACGGGCCGAA AGGGATCGGC GCGCTGCTCG TGCGCGACGG CGTGCCGCTC GCGGCGCAGT TTCCCGGCAG CCAGGAGCGG CACCGGCGCG GCGGCACCGA AAACCTGCCC GCGATCGTCG GCTTCGCCGC GGCGCTCGAG CGGCTGGCCG ATGTGCTCGC CGACGCCGCA CGGATCGCCG CGCTGCGCGA CGCGTTCGAG GCCGGCCTGC GCGCGGCGCT GCCCGGCGTG CACGTGTACG GCGCGAGCGC GCCGCGCTTG CCCGGCACGA GCTGCCTGCG CGTCGGCCGA CTGGACGCCG ACACCGTCTT GCAGCGCTGC GAGCAGATCG GCGTGTGCGC GTCGTCAGGC TCGGCGTGCT CGTCCGGCGG CAGCGAGCCG TCGCACGTGC TGAGCGCGAT GGGCGTGCCG CGCGACGAGG CGCTCGGCGC CGTGCGCTTC TCGCTCGGCG CGGCCACCAC CGCGCGCGAC ATCGATTACC TGCTCGCCGC GCTGCCTCCG TTGTTGAAGC CGTTGCTGGC CGAAGCGGCC GTGCAGCCGA CC
Details for BuviA.00081.b.B1.GE35354
HARVESTED ON: 5/16/2012
SEQUENCED ON: 5/15/2012
EXPECTED MW: 39kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMI YLDHNATTAP APAAIDAMLA VLTDVWANAS SQHGAGQXAK RTLAAARATI AXALGCKARE VVFTSGATEA NHLAVLGLGA SATASAVRRR LVFGATXHAA QLKLAHALAA QGRPVDFIAV RPDGVLDLDS AARVIGPDVA LVSVMAANNE TGVLMPIADV KXLXXXAGAL LHVDATXLVG KLPXDFAALG AEXXSLXAXX XXXXXXXXX
Validated NT Sequence
gnnnnntttn nnaaggagan ataccatggc tcaccaccac caccaccata tgatctatct cgaccacaac gcgacgacgg cgcccgcgcc ggcggcgatc gacgcgatgc tcgccgtgct gaccgacgtg tgggccaacg cgtcgtcgca gcatggcgcc ggacagcang cgaagcgcac gctggccgcc gcgcgcgcga cgatcgccng ngcnctcggt tgcaaggcca gggaagtggt cttcacgagc ggcgcgaccg aggcgaatca tctcgcggtg ctcgggctcg gggcgtcggc cacggcctcg gccgtgcggc gcaggctcgt cttcggcgcg acggancatg cggcgcagct gaagctcgcg cacgcgctcg ccgcccaggg ccgtccggtc gatttcatcg cggtgcggcc ggacggcgtg ctcgatctcg acagcgccgc gcgcgtgatc ggnccggacg tcgcgctggt gtcggtgatg gcggcgaaca acgagaccgg cgtgctgatg ccnatcgccg acgtgaaggn gctcgnncan gnggcgggcg cgctgctgca cgtcgacgcg acgcanctgg tcggnaagct gccgntcgat ttcgcggcgc tcggcgccga gnncttntcg ctnnnngcgn ncannnncnn nngnnnncnn nnnnnnnnnn ntg
Expected Protein Sequence
MAHHHHHHMI YLDHNATTAP APAAIDAMLA VLTDVWANAS SQHGAGQQAK RTLAAARATI AGALGCKARE VVFTSGATEA NHLAVLGLGA SATASAVRRR LVFGATEHAA QLKLAHALAA QGRPVDFIAV RPDGVLDLDS AARVIGPDVA LVSVMAANNE TGVLMPIADV KALAQAAGAL LHVDATQLVG KLPFDFAALG ADAVSLSAHK LHGPKGIGAL LVRDGVPLAA QFPGSQERHR RGGTENLPAI VGFAAALERL ADVLADAARI AALRDAFEAG LRAALPGVHV YGASAPRLPG TSCLRVGRLD ADTVLQRCEQ IGVCASSGSA CSSGGSEPSH VLSAMGVPRD EALGAVRFSL GAATTARDID YLLAALPPLL KPLLAEAAVQ PT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatct atctcgacca caacgcgacg acggcgcccg cgccggcggc gatcgacgcg atgctcgccg tgctgaccga cgtgtgggcc aacgcgtcgt cgcagcatgg cgccggacag caggcgaagc gcacgctggc cgccgcgcgc gcgacgatcg ccggcgcgct cggttgcaag gccagggaag tggtcttcac gagcggcgcg accgaggcga atcatctcgc ggtgctcggg ctcggggcgt cggccacggc ctcggccgtg cggcgcaggc tcgtcttcgg cgcgacggag catgcggcgc agctgaagct cgcgcacgcg ctcgccgccc agggccgtcc ggtcgatttc atcgcggtgc ggccggacgg cgtgctcgat ctcgacagcg ccgcgcgcgt gatcggcccg gacgtcgcgc tggtgtcggt gatggcggcg aacaacgaga ccggcgtgct gatgccgatc gccgacgtga aggcgctcgc gcaggcggcg ggcgcgctgc tgcacgtcga cgcgacgcag ctggtcggca agctgccgtt cgatttcgcg gcgctcggcg ccgacgcggt gtcgctgtcc gcgcacaagc tgcacgggcc gaaagggatc ggcgcgctgc tcgtgcgcga cggcgtgccg ctcgcggcgc agtttcccgg cagccaggag cggcaccggc gcggcggcac cgaaaacctg cccgcgatcg tcggcttcgc cgcggcgctc gagcggctgg ccgatgtgct cgccgacgcc gcacggatcg ccgcgctgcg cgacgcgttc gaggccggcc tgcgcgcggc gctgcccggc gtgcacgtgt acggcgcgag cgcgccgcgc ttgcccggca cgagctgcct gcgcgtcggc cgactggacg ccgacaccgt cttgcagcgc tgcgagcaga tcggcgtgtg cgcgtcgtca ggctcggcgt gctcgtccgg cggcagcgag ccgtcgcacg tgctgagcgc gatgggcgtg ccgcgcgacg aggcgctcgg cgccgtgcgc ttctcgctcg gcgcggccac caccgcgcgc gacatcgatt acctgctcgc cgcgctgcct ccgttgttga agccgttgct ggccgaagcg gccgtgcagc cgacctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuviA.00081.b.B1.PW35978
PURIFICATION DATe: 8/20/2012
CONCENTRATION: 33mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 89
PERCENT COVERAGE: 55
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI YLDHNATTAP APAAIDAMLA VLTDVWANAS SQHGAGQXAK RTLAAARATI AXALGCKARE VVFTSGATEA NHLAVLGLGA SATASAVRRR LVFGATXHAA QLKLAHALAA QGRPVDFIAV RPDGVLDLDS AARVIGPDVA LVSVMAANNE TGVLMPIADV KXLXXXAGAL LHVDATXLVG KLPXDFAALG AEXXSLXAXX XXXXXXXXX
Validated NT Sequence
gnnnnntttn nnaaggagan ataccatggc tcaccaccac caccaccata tgatctatct cgaccacaac gcgacgacgg cgcccgcgcc ggcggcgatc gacgcgatgc tcgccgtgct gaccgacgtg tgggccaacg cgtcgtcgca gcatggcgcc ggacagcang cgaagcgcac gctggccgcc gcgcgcgcga cgatcgccng ngcnctcggt tgcaaggcca gggaagtggt cttcacgagc ggcgcgaccg aggcgaatca tctcgcggtg ctcgggctcg gggcgtcggc cacggcctcg gccgtgcggc gcaggctcgt cttcggcgcg acggancatg cggcgcagct gaagctcgcg cacgcgctcg ccgcccaggg ccgtccggtc gatttcatcg cggtgcggcc ggacggcgtg ctcgatctcg acagcgccgc gcgcgtgatc ggnccggacg tcgcgctggt gtcggtgatg gcggcgaaca acgagaccgg cgtgctgatg ccnatcgccg acgtgaaggn gctcgnncan gnggcgggcg cgctgctgca cgtcgacgcg acgcanctgg tcggnaagct gccgntcgat ttcgcggcgc tcggcgccga gnncttntcg ctnnnngcgn ncannnncnn nngnnnncnn nnnnnnnnnn ntg
Expressed Protein Sequence
MAHHHHHHMI YLDHNATTAP APAAIDAMLA VLTDVWANAS SQHGAGQQAK RTLAAARATI AGALGCKARE VVFTSGATEA NHLAVLGLGA SATASAVRRR LVFGATEHAA QLKLAHALAA QGRPVDFIAV RPDGVLDLDS AARVIGPDVA LVSVMAANNE TGVLMPIADV KALAQAAGAL LHVDATQLVG KLPFDFAALG ADAVSLSAHK LHGPKGIGAL LVRDGVPLAA QFPGSQERHR RGGTENLPAI VGFAAALERL ADVLADAARI AALRDAFEAG LRAALPGVHV YGASAPRLPG TSCLRVGRLD ADTVLQRCEQ IGVCASSGSA CSSGGSEPSH VLSAMGVPRD EALGAVRFSL GAATTARDID YLLAALPPLL KPLLAEAAVQ PT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCT ATCTCGACCA CAACGCGACG ACGGCGCCCG CGCCGGCGGC GATCGACGCG ATGCTCGCCG TGCTGACCGA CGTGTGGGCC AACGCGTCGT CGCAGCATGG CGCCGGACAG CAGGCGAAGC GCACGCTGGC CGCCGCGCGC GCGACGATCG CCGGCGCGCT CGGTTGCAAG GCCAGGGAAG TGGTCTTCAC GAGCGGCGCG ACCGAGGCGA ATCATCTCGC GGTGCTCGGG CTCGGGGCGT CGGCCACGGC CTCGGCCGTG CGGCGCAGGC TCGTCTTCGG CGCGACGGAG CATGCGGCGC AGCTGAAGCT CGCGCACGCG CTCGCCGCCC AGGGCCGTCC GGTCGATTTC ATCGCGGTGC GGCCGGACGG CGTGCTCGAT CTCGACAGCG CCGCGCGCGT GATCGGCCCG GACGTCGCGC TGGTGTCGGT GATGGCGGCG AACAACGAGA CCGGCGTGCT GATGCCGATC GCCGACGTGA AGGCGCTCGC GCAGGCGGCG GGCGCGCTGC TGCACGTCGA CGCGACGCAG CTGGTCGGCA AGCTGCCGTT CGATTTCGCG GCGCTCGGCG CCGACGCGGT GTCGCTGTCC GCGCACAAGC TGCACGGGCC GAAAGGGATC GGCGCGCTGC TCGTGCGCGA CGGCGTGCCG CTCGCGGCGC AGTTTCCCGG CAGCCAGGAG CGGCACCGGC GCGGCGGCAC CGAAAACCTG CCCGCGATCG TCGGCTTCGC CGCGGCGCTC GAGCGGCTGG CCGATGTGCT CGCCGACGCC GCACGGATCG CCGCGCTGCG CGACGCGTTC GAGGCCGGCC TGCGCGCGGC GCTGCCCGGC GTGCACGTGT ACGGCGCGAG CGCGCCGCGC TTGCCCGGCA CGAGCTGCCT GCGCGTCGGC CGACTGGACG CCGACACCGT CTTGCAGCGC TGCGAGCAGA TCGGCGTGTG CGCGTCGTCA GGCTCGGCGT GCTCGTCCGG CGGCAGCGAG CCGTCGCACG TGCTGAGCGC GATGGGCGTG CCGCGCGACG AGGCGCTCGG CGCCGTGCGC TTCTCGCTCG GCGCGGCCAC CACCGCGCGC GACATCGATT ACCTGCTCGC CGCGCTGCCT CCGTTGTTGA AGCCGTTGCT GGCCGAAGCG GCCGTGCAGC CGACCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT