SSGCID: Target Page

BuviA.00081.a

Aminotransferase, class V

CENTER ID:	BuviA.00081.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00081.a.B1.GE35355	Full length( BuviA.00081.a )		1	494

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00081.a.B1.PS01587	Full length( BuviA.00081.a )		1	494

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.2678
RefSeq:	YP_001116395.1
UniProt:	A4JKX7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDTTPLHYL DYAATTPADP RVIEAMMACL GADGIFGNPA SSSHAAGRLA KQKVEHARAQ VAALIGADAD EIVWTSGATE SNNLALKGYA ETATGKRHLI TSRLEHKAIL DTTANLSKHG MTVTYLSPNA DGEITADAVA AALSADTGLV SLMLVNNELG TLTDIGAISR IVHAAGALLH VDAAQALGKT PIDVRALGID LLSMSAHKVY GPKGIGALFV RRDIADRIAP QIHGGGHERG LRSGTLATHQ IVGMGVACEL AADKLEREAA RIAALSARVK DALLALGDIT HNAAAARRIP HTMSFTVDVP GFFPFMLGDA LAVSSTSACN STSGAPSHVL TAIGLDAAAA SRTVRISFGR FTTEQDAEFA IACFARALEQ CRAAAATGFT ASRQITPAEL TAIRHAGFRA VICNRPDGES ADQPAFDEIA AAARELGLDA RYLPVERDGI DDAQVDAFGA LVDALPKPVL AYCRSGSRSA LLWNRLTERR AITV

NT Sequence

ATGAGCGATA CCACCCCGCT GCACTACCTC GACTACGCCG CCACCACGCC CGCCGATCCG CGCGTGATCG AAGCGATGAT GGCCTGCCTC GGCGCCGACG GCATCTTCGG CAACCCGGCA TCGAGCTCGC ACGCCGCCGG CCGGCTGGCG AAGCAGAAGG TCGAGCACGC ACGCGCCCAG GTCGCGGCGC TGATCGGCGC GGACGCCGAC GAGATCGTCT GGACCTCGGG CGCGACCGAA TCGAACAACC TCGCGCTGAA GGGCTACGCG GAAACCGCGA CCGGCAAGCG CCACCTGATC ACGAGCCGCC TCGAACACAA GGCGATCCTC GACACGACGG CGAACCTGTC GAAGCACGGA ATGACGGTCA CCTATCTGTC GCCGAACGCC GACGGCGAAA TCACCGCCGA CGCCGTAGCC GCGGCACTCA GCGCCGATAC GGGGCTCGTG TCGCTGATGC TCGTCAACAA CGAGCTGGGC ACGCTGACCG ACATCGGCGC GATCTCGCGC ATCGTCCACG CGGCCGGCGC GCTGTTGCAC GTCGACGCCG CGCAGGCGCT CGGCAAGACG CCGATCGACG TGCGCGCGCT CGGTATCGAC CTGCTGTCGA TGTCCGCGCA CAAGGTATAT GGCCCGAAAG GGATCGGCGC GCTGTTCGTC CGCCGCGACA TCGCGGATCG CATCGCGCCG CAGATCCACG GCGGCGGACA CGAGCGCGGC CTGCGTTCGG GCACGCTCGC GACGCATCAG ATCGTCGGGA TGGGCGTCGC GTGTGAATTG GCCGCCGACA AGCTCGAGCG CGAAGCCGCA CGGATCGCGG CGCTCAGCGC ACGCGTGAAG GACGCGCTGC TCGCGCTCGG CGACATCACG CACAACGCGG CAGCGGCGCG CCGCATTCCG CACACGATGA GCTTCACCGT CGACGTGCCG GGCTTCTTCC CGTTCATGCT CGGCGATGCG CTCGCGGTGT CGTCGACGTC CGCGTGCAAC TCGACGAGCG GTGCGCCGTC GCACGTGCTG ACCGCGATCG GCCTCGATGC CGCCGCCGCG AGCCGCACGG TGCGCATCAG CTTCGGCCGC TTCACGACGG AACAGGACGC CGAGTTCGCG ATCGCGTGTT TCGCTCGCGC GCTCGAACAA TGCCGGGCCG CGGCCGCGAC CGGCTTCACC GCGTCGCGGC AGATCACGCC GGCCGAGCTG ACGGCGATTC GTCATGCGGG CTTCCGTGCG GTGATCTGCA ACCGGCCGGA CGGCGAAAGC GCCGACCAGC CGGCATTCGA CGAGATCGCC GCCGCGGCGC GTGAACTCGG GCTCGACGCG CGCTATCTGC CGGTCGAGCG CGACGGGATC GACGATGCGC AGGTCGACGC GTTCGGCGCG CTGGTCGATG CGCTGCCGAA GCCGGTGCTC GCGTATTGTC GAAGCGGCAG CCGCTCCGCG CTGCTGTGGA ACCGGCTGAC CGAGCGCCGC GCGATCACGG TT

Details for BuviA.00081.a.B1.GE35355

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Validated AA Sequence

MMACLGADGI FGNPASSSHA AGRLAKQKVE HARAQVAALI GADADEIVWT SGATESNNLA LKGYAETATG KRHLITSRLE HKAILDTTAN LSKHGMTVTY LSPNADGEIT ADAVAAALSA DTGLVSLMLV NNELGTLTDI GAISRIVHAA GALLHVDAAQ ALGKTPIDVR ALGIDLLSMS AHKVYGPKGI GALFVRRDIA DRIAPQIHGG GHERGLRSGT LATHQIVGMG VACELAADKL EREAARIAAL SARVKDALLA LGDITHNAAA ARRIPHTMSF TVDVPGFFPF MLGDALAVSS TSACNSTSGA PSHVLTAIGL DAAAASRTVR ISFGRFTTEQ DAEFAIACFA RALEQCRAAA ATGFTASRQI TPAELTAIRH AGFRAVICNR PDGESADQPA FDEIAAAARE LGLDARYLPV ERDGIDDAQV DAFGALVDAL PKPVLAYCRS GSRSALLWNR

Validated NT Sequence

agccggttcc acagcagcgc ggagcggctg ccgcttcgac aatacgcgag caccggcttc ggcagcgcat cgaccagcgc gccgaacgcg tcgacctgcg catcgtcgat cccgtcgcgc tcgaccggca gatagcgcgc gtcgagcccg agttcacgcg ccgcggcggc gatctcgtcg aatgccggct ggtcggcgct ttcgccgtcc ggccggttgc agatcaccgc acggaagccc gcatgacgaa tcgccgtcag ctcggccggc gtgatctgcc gcgacgcggt gaagccggtc gcggccgcgg cccggcattg ttcgagcgcg cgagcgaaac acgcgatcgc gaactcggcg tcctgttccg tcgtgaagcg gccgaagctg atgcgcaccg tgcggctcgc ggcggcggca tcgaggccga tcgcggtcag cacgtgcgac ggcgcaccgc tcgtcgagtt gcacgcggac gtcgacgaca ccgcgagcgc atcgccgagc atgaacggga agaagcccgg cacgtcgacg gtgaagctca tcgtgtgcgg aatgcggcgc gccgctgccg cgttgtgcgt gatgtcgccg agcgcgagca gcgcgtcctt cacgcgtgcg ctgagcgccg cgatccgtgc ggcttcgcgc tcgagcttgt cggcggccaa ttcacacgcg acgcccatcc cgacgatctg atgcgtcgcg agcgtgcccg aacgcaggcc gcgctcgtgt ccgccgccgt ggatctgcgg cgcgatgcga tccgcgatgt cgcggcggac gaacagcgcg ccgatccctt tcgggccata taccttgtgc gcggacatcg acagcaggtc gataccgagc gcgcgcacgt cgatcggcgt cttgccgagc gcctgcgcgg cgtcgacgtg caacagcgcg ccggccgcgt ggacgatgcg cgagatcgcg ccgatgtcgg tcagcgtgcc cagctcgttg ttgacgagca tcagcgacac gagccccgta tcggcgctga gtgccgcggc tacggcgtcg gcggtgattt cgccgtcggc gttcggcgac agataggtga ccgtcattcc gtgcttcgac aggttcgccg tcgtgtcgag gatcgccttg tgttcgaggc ggctcgtgat caggtggcgc ttgccggtcg cggtttccgc gtagcccttc agcgcgaggt tgttcgattc ggtcgcgccc gaggtccaga cgatctcgtc ggcgtccgcg ccgatcagcg ccgcgacctg ggcgcgtgcg tgctcgacct tctgcttcgc cagccggccg gcggcgtgcg agctcgatgc cgggttgccg aagatgccgt cggcgccgag gcaggccatc atcgcttcga tcacgcgcgg atcggcgggc gtggtggcgg cgtagtcgag gtagtgcagc ggggtggtat cg

Expected Protein Sequence

MAHHHHHHMS DTTPLHYLDY AATTPADPRV IEAMMACLGA DGIFGNPASS SHAAGRLAKQ KVEHARAQVA ALIGADADEI VWTSGATESN NLALKGYAET ATGKRHLITS RLEHKAILDT TANLSKHGMT VTYLSPNADG EITADAVAAA LSADTGLVSL MLVNNELGTL TDIGAISRIV HAAGALLHVD AAQALGKTPI DVRALGIDLL SMSAHKVYGP KGIGALFVRR DIADRIAPQI HGGGHERGLR SGTLATHQIV GMGVACELAA DKLEREAARI AALSARVKDA LLALGDITHN AAAARRIPHT MSFTVDVPGF FPFMLGDALA VSSTSACNST SGAPSHVLTA IGLDAAAASR TVRISFGRFT TEQDAEFAIA CFARALEQCR AAAATGFTAS RQITPAELTA IRHAGFRAVI CNRPDGESAD QPAFDEIAAA ARELGLDARY LPVERDGIDD AQVDAFGALV DALPKPVLAY CRSGSRSALL WNRLTERRAI TV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ataccacccc gctgcactac ctcgactacg ccgccaccac gcccgccgat ccgcgcgtga tcgaagcgat gatggcctgc ctcggcgccg acggcatctt cggcaacccg gcatcgagct cgcacgccgc cggccggctg gcgaagcaga aggtcgagca cgcacgcgcc caggtcgcgg cgctgatcgg cgcggacgcc gacgagatcg tctggacctc gggcgcgacc gaatcgaaca acctcgcgct gaagggctac gcggaaaccg cgaccggcaa gcgccacctg atcacgagcc gcctcgaaca caaggcgatc ctcgacacga cggcgaacct gtcgaagcac ggaatgacgg tcacctatct gtcgccgaac gccgacggcg aaatcaccgc cgacgccgta gccgcggcac tcagcgccga tacggggctc gtgtcgctga tgctcgtcaa caacgagctg ggcacgctga ccgacatcgg cgcgatctcg cgcatcgtcc acgcggccgg cgcgctgttg cacgtcgacg ccgcgcaggc gctcggcaag acgccgatcg acgtgcgcgc gctcggtatc gacctgctgt cgatgtccgc gcacaaggta tatggcccga aagggatcgg cgcgctgttc gtccgccgcg acatcgcgga tcgcatcgcg ccgcagatcc acggcggcgg acacgagcgc ggcctgcgtt cgggcacgct cgcgacgcat cagatcgtcg ggatgggcgt cgcgtgtgaa ttggccgccg acaagctcga gcgcgaagcc gcacggatcg cggcgctcag cgcacgcgtg aaggacgcgc tgctcgcgct cggcgacatc acgcacaacg cggcagcggc gcgccgcatt ccgcacacga tgagcttcac cgtcgacgtg ccgggcttct tcccgttcat gctcggcgat gcgctcgcgg tgtcgtcgac gtccgcgtgc aactcgacga gcggtgcgcc gtcgcacgtg ctgaccgcga tcggcctcga tgccgccgcc gcgagccgca cggtgcgcat cagcttcggc cgcttcacga cggaacagga cgccgagttc gcgatcgcgt gtttcgctcg cgcgctcgaa caatgccggg ccgcggccgc gaccggcttc accgcgtcgc ggcagatcac gccggccgag ctgacggcga ttcgtcatgc gggcttccgt gcggtgatct gcaaccggcc ggacggcgaa agcgccgacc agccggcatt cgacgagatc gccgccgcgg cgcgtgaact cgggctcgac gcgcgctatc tgccggtcga gcgcgacggg atcgacgatg cgcaggtcga cgcgttcggc gcgctggtcg atgcgctgcc gaagccggtg ctcgcgtatt gtcgaagcgg cagccgctcc gcgctgctgt ggaaccggct gaccgagcgc cgcgcgatca cggtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuviA.00081.a.B1.PS01587

PURIFICATION DATe:	8/7/2012
CONCENTRATION:	56.04mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ATACCACCCC GCTGCACTAC CTCGACTACG CCGCCACCAC GCCCGCCGAT CCGCGCGTGA TCGAAGCGAT GATGGCCTGC CTCGGCGCCG ACGGCATCTT CGGCAACCCG GCATCGAGCT CGCACGCCGC CGGCCGGCTG GCGAAGCAGA AGGTCGAGCA CGCACGCGCC CAGGTCGCGG CGCTGATCGG CGCGGACGCC GACGAGATCG TCTGGACCTC GGGCGCGACC GAATCGAACA ACCTCGCGCT GAAGGGCTAC GCGGAAACCG CGACCGGCAA GCGCCACCTG ATCACGAGCC GCCTCGAACA CAAGGCGATC CTCGACACGA CGGCGAACCT GTCGAAGCAC GGAATGACGG TCACCTATCT GTCGCCGAAC GCCGACGGCG AAATCACCGC CGACGCCGTA GCCGCGGCAC TCAGCGCCGA TACGGGGCTC GTGTCGCTGA TGCTCGTCAA CAACGAGCTG GGCACGCTGA CCGACATCGG CGCGATCTCG CGCATCGTCC ACGCGGCCGG CGCGCTGTTG CACGTCGACG CCGCGCAGGC GCTCGGCAAG ACGCCGATCG ACGTGCGCGC GCTCGGTATC GACCTGCTGT CGATGTCCGC GCACAAGGTA TATGGCCCGA AAGGGATCGG CGCGCTGTTC GTCCGCCGCG ACATCGCGGA TCGCATCGCG CCGCAGATCC ACGGCGGCGG ACACGAGCGC GGCCTGCGTT CGGGCACGCT CGCGACGCAT CAGATCGTCG GGATGGGCGT CGCGTGTGAA TTGGCCGCCG ACAAGCTCGA GCGCGAAGCC GCACGGATCG CGGCGCTCAG CGCACGCGTG AAGGACGCGC TGCTCGCGCT CGGCGACATC ACGCACAACG CGGCAGCGGC GCGCCGCATT CCGCACACGA TGAGCTTCAC CGTCGACGTG CCGGGCTTCT TCCCGTTCAT GCTCGGCGAT GCGCTCGCGG TGTCGTCGAC GTCCGCGTGC AACTCGACGA GCGGTGCGCC GTCGCACGTG CTGACCGCGA TCGGCCTCGA TGCCGCCGCC GCGAGCCGCA CGGTGCGCAT CAGCTTCGGC CGCTTCACGA CGGAACAGGA CGCCGAGTTC GCGATCGCGT GTTTCGCTCG CGCGCTCGAA CAATGCCGGG CCGCGGCCGC GACCGGCTTC ACCGCGTCGC GGCAGATCAC GCCGGCCGAG CTGACGGCGA TTCGTCATGC GGGCTTCCGT GCGGTGATCT GCAACCGGCC GGACGGCGAA AGCGCCGACC AGCCGGCATT CGACGAGATC GCCGCCGCGG CGCGTGAACT CGGGCTCGAC GCGCGCTATC TGCCGGTCGA GCGCGACGGG ATCGACGATG CGCAGGTCGA CGCGTTCGGC GCGCTGGTCG ATGCGCTGCC GAAGCCGGTG CTCGCGTATT GTCGAAGCGG CAGCCGCTCC GCGCTGCTGT GGAACCGGCT GACCGAGCGC CGCGCGATCA CGGTTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT