BuviA.00077.a

Fumarylacetoacetate hydrolase

CENTER ID: BuviA.00077.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00077.a.B1.GE38093 Full length( BuviA.00077.a ) 1 434
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.4974
RefSeq: YP_001118605.1
UniProt: A4JBW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTRDWRAT LDPARKSWVD TANDPACDFP IQNLPFGIFS DAAQPARRAG VALGDRIVDL AALARAGLVT VPAGADVFAE PTLNAFIALG RDAWRSVRIQ LSELFARDAA RLRDDAALRA RVLVAQRDAT LHLPVDIPGY TDFYSSKEHA TNVGSMFRDP KNALLPNWSE MPIGYNGRAS SVVVSGTPVR RPNGQLKLPE QERPVFGACR KLDIELETGF IVGRGNALGE PIACEDAEAH IFGMVLLNDW SARDIQQWEY VPLGPFNSKG FATTISPWIV TLDALEPFRV AQPEQAPQPL PYLRHAGKHA FDIALEVTLR ADGAAEPTSI CRTNFRHMYW TMAQQLAHHT VAGCNTRVGD LMGSGTISGP TRDSFGSLLE LTWNGKEPLV LKGGGSRTFI EDGDELTLAG WCEGDGYRIG FGTCTGKILP ARSA
NT Sequence
ATGAGCGACA CCCGAGACTG GCGTGCGACG CTCGACCCGG CTCGCAAGAG CTGGGTCGAC ACGGCGAACG ATCCCGCCTG CGATTTCCCG ATCCAGAACC TGCCGTTCGG CATCTTCAGC GACGCGGCGC AGCCGGCGCG CCGCGCGGGC GTGGCGCTCG GCGACCGGAT CGTCGACCTC GCCGCGCTCG CGCGTGCGGG CCTCGTGACG GTGCCGGCCG GCGCCGACGT GTTCGCCGAG CCGACGCTCA ACGCGTTCAT CGCGCTCGGC CGCGATGCGT GGCGCAGCGT GCGCATTCAG CTGTCCGAGC TGTTCGCGCG CGACGCTGCG CGGCTCCGCG ACGACGCGGC GTTGCGTGCG CGCGTGCTCG TCGCGCAGCG CGACGCGACG CTGCATCTGC CGGTCGACAT TCCCGGCTAC ACCGATTTCT ATTCGTCGAA GGAGCACGCG ACCAACGTCG GCTCGATGTT CCGCGATCCG AAGAATGCGC TGCTGCCGAA CTGGTCGGAG ATGCCGATCG GCTACAACGG CCGCGCGTCA TCGGTGGTCG TGAGCGGCAC GCCGGTGCGG CGGCCGAACG GGCAGCTGAA GCTGCCCGAG CAGGAGCGTC CGGTGTTCGG CGCGTGCCGC AAGCTCGACA TCGAACTCGA GACGGGCTTC ATCGTCGGGC GGGGCAATGC GCTCGGCGAG CCGATCGCCT GCGAGGATGC CGAAGCGCAC ATCTTCGGGA TGGTGCTGTT GAACGACTGG AGCGCGCGCG ACATCCAGCA GTGGGAATAC GTGCCGCTCG GCCCATTCAA CTCGAAGGGC TTCGCGACGA CGATCTCGCC GTGGATCGTC ACGCTCGACG CGCTCGAACC GTTCCGCGTC GCGCAGCCCG AGCAGGCGCC GCAGCCGCTG CCGTATCTGC GGCACGCGGG CAAGCATGCG TTCGACATCG CGCTCGAGGT TACGCTGCGG GCGGACGGCG CCGCCGAGCC GACGTCGATC TGCCGCACCA ACTTCCGGCA CATGTACTGG ACGATGGCGC AGCAACTCGC GCATCACACG GTCGCGGGCT GCAATACGCG CGTGGGGGAC CTGATGGGTT CGGGCACGAT CAGCGGGCCG ACGCGCGACT CGTTCGGCAG CCTGCTCGAG CTGACGTGGA ACGGCAAGGA GCCGCTCGTG CTGAAGGGCG GCGGCAGCCG CACGTTCATC GAGGACGGCG ACGAGCTGAC GCTCGCGGGC TGGTGCGAGG GCGATGGCTA TCGCATCGGC TTCGGGACGT GTACGGGGAA GATCCTGCCG GCGCGTAGCG CG
Details for BuviA.00077.a.B1.GE38093
HARVESTED ON: 5/13/2014
SEQUENCED ON: 5/6/2014
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 63
Validated AA Sequence
MFRDPKNALL PNWSEMPIGY NGRASSVVVS GTPVRRPNGQ LKLPEQERPV FGACRKLDIE LETGFIVGRG NALGEPIACE DAEAHIFGMV LLNDWSARDI QQWEYVPLGP FNSKGFATTI SPWIVTLDAL EPFRVAQPEQ APQPLPYLRH AGKHAFDIAL EVTLRADGAA EPTSICRTNF RHMYWTMAQQ LAHHTVAGCN TRVGDLMGSG TISGPTRDSF GSLLELTWNG KEPLVLKGGG SRTFIEDGDE LTLAGWCEGD GYRIGFGTCT GKILPARSA
Validated NT Sequence
atcttactca cttacgcgct acgcgccggc aggatcttcc ccgtacacgt cccgaagccg atgcgatagc catcgccctc gcaccagccc gcgagcgtca gctcgtcgcc gtcctcgatg aacgtgcggc tgccgccgcc cttcagcacg agcggctcct tgccgttcca cgtcagctcg agcaggctgc cgaacgagtc gcgcgtcggc ccgctgatcg tgcccgaacc catcaggtcc cccacgcgcg tattgcagcc cgcgaccgtg tgatgcgcga gttgctgcgc catcgtccag tacatgtgcc ggaagttggt gcggcagatc gacgtcggct cggcggcgcc gtccgcccgc agcgtaacct cgagcgcgat gtcgaacgca tgcttgcccg cgtgccgcag atacggcagc ggctgcggcg cctgctcggg ctgcgcgacg cggaacggtt cgagcgcgtc gagcgtgacg atccacggcg agatcgtcgt cgcgaagccc ttcgagttga atgggccgag cggcacgtat tcccactgct ggatgtcgcg cgcgctccag tcgttcaaca gcaccatccc gaagatgtgc gcttcggcat cctcgcaggc gatcggctcg ccgagcgcat tgccccgccc gacgatgaag cccgtctcga gttcgatgtc gagcttgcgg cacgcgccga acaccggacg ctcctgctcg ggcagcttca gctgcccgtt cggccgccgc accggcgtgc cgctcacgac caccgatgac gcgcggccgt tgtagccgat cggcatctcc gaccagttcg gcagcagcgc attcttcgga tcgcggaaca tcgagccgac gttggtcgcg tgctccttcg acgaatagaa atcggtgtag ccgngaatgt cgaccggcag atgcagcgtc ncgtcgcgct gcgcnangag cacgcgcgca cgcnacgncg cgtcgtcgcg nnnncncgca ncntcgcnnn nnancagctc ggacanctna atgcgcacgc tgcgccncgc nnnnngnnnn nnnntnntnn nnnnnnnnng cntnnnntnn nngnncnnnn nnnnnnnnnn nnnnnnngnn nngannnccn nnnnnnnnnn cncgnnnagg tngncgatcn nntnnnngan cgccacgccc gcgnggcgcn ccggctgcgc cgcgtcgctg aagatgccga acggcaggtt ctggatcggg aaatcgcagg cgggatcgtt cgccgtgtcg acccagctct tgcgagccgg gtcgagcgtc gcacgccagt ctcgggtgtc gctc
Expected Protein Sequence
MAHHHHHHMS DTRDWRATLD PARKSWVDTA NDPACDFPIQ NLPFGIFSDA AQPARRAGVA LGDRIVDLAA LARAGLVTVP AGADVFAEPT LNAFIALGRD AWRSVRIQLS ELFARDAARL RDDAALRARV LVAQRDATLH LPVDIPGYTD FYSSKEHATN VGSMFRDPKN ALLPNWSEMP IGYNGRASSV VVSGTPVRRP NGQLKLPEQE RPVFGACRKL DIELETGFIV GRGNALGEPI ACEDAEAHIF GMVLLNDWSA RDIQQWEYVP LGPFNSKGFA TTISPWIVTL DALEPFRVAQ PEQAPQPLPY LRHAGKHAFD IALEVTLRAD GAAEPTSICR TNFRHMYWTM AQQLAHHTVA GCNTRVGDLM GSGTISGPTR DSFGSLLELT WNGKEPLVLK GGGSRTFIED GDELTLAGWC EGDGYRIGFG TCTGKILPAR SA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acacccgaga ctggcgtgcg acgctcgacc cggctcgcaa gagctgggtc gacacggcga acgatcccgc ctgcgatttc ccgatccaga acctgccgtt cggcatcttc agcgacgcgg cgcagccggc gcgccgcgcg ggcgtggcgc tcggcgaccg gatcgtcgac ctcgccgcgc tcgcgcgtgc gggcctcgtg acggtgccgg ccggcgccga cgtgttcgcc gagccgacgc tcaacgcgtt catcgcgctc ggccgcgatg cgtggcgcag cgtgcgcatt cagctgtccg agctgttcgc gcgcgacgct gcgcggctcc gcgacgacgc ggcgttgcgt gcgcgcgtgc tcgtcgcgca gcgcgacgcg acgctgcatc tgccggtcga cattcccggc tacaccgatt tctattcgtc gaaggagcac gcgaccaacg tcggctcgat gttccgcgat ccgaagaatg cgctgctgcc gaactggtcg gagatgccga tcggctacaa cggccgcgcg tcatcggtgg tcgtgagcgg cacgccggtg cggcggccga acgggcagct gaagctgccc gagcaggagc gtccggtgtt cggcgcgtgc cgcaagctcg acatcgaact cgagacgggc ttcatcgtcg ggcggggcaa tgcgctcggc gagccgatcg cctgcgagga tgccgaagcg cacatcttcg ggatggtgct gttgaacgac tggagcgcgc gcgacatcca gcagtgggaa tacgtgccgc tcggcccatt caactcgaag ggcttcgcga cgacgatctc gccgtggatc gtcacgctcg acgcgctcga accgttccgc gtcgcgcagc ccgagcaggc gccgcagccg ctgccgtatc tgcggcacgc gggcaagcat gcgttcgaca tcgcgctcga ggttacgctg cgggcggacg gcgccgccga gccgacgtcg atctgccgca ccaacttccg gcacatgtac tggacgatgg cgcagcaact cgcgcatcac acggtcgcgg gctgcaatac gcgcgtgggg gacctgatgg gttcgggcac gatcagcggg ccgacgcgcg actcgttcgg cagcctgctc gagctgacgt ggaacggcaa ggagccgctc gtgctgaagg gcggcggcag ccgcacgttc atcgaggacg gcgacgagct gacgctcgcg ggctggtgcg agggcgatgg ctatcgcatc ggcttcggga cgtgtacggg gaagatcctg ccggcgcgta gcgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat