SSGCID: Target Page

BuviA.00068.a

Phenylalanine 4-hydroxylase

CENTER ID:	BuviA.00068.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00068.a.B1.GE38092	Full length( BuviA.00068.a )		1	327

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.4293
RefSeq:	YP_001117946.1
UniProt:	A4JA08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENRRHLCAH HARAPLYTRM VIHPSMQSVT MSTVVTAKLQ EQFDAGLETR ADFTIDQPLE RYGQVDHAVW QQLYARQAAL LRGRACDAFI GGLGKIRLPA DRVPSFADVN RQLKPATGWE IVAVPGLVPD RVFFEHLAHR RFPVTWWMRR PDQLDYLQEP DCFHDLFGHV PLLIEPVFAD YMQAYGRTAL AVADDDAALA RLARLYWYTV EFGLIRDPRG TNGLSIYGAG IVSSKGESLY SVESAAPNRL GFDLERVMRT KYRIDTFQKT YFVIDDFAQL FALADVDARA LAERLAALPE LSAGTVLDTD VVLQRGTGEG WPDDDDA

NT Sequence

ATGGAGAATC GACGCCATCT TTGCGCCCAT CATGCGCGCG CCCCGCTCTA CACTCGCATG GTCATTCACC CAAGCATGCA GAGCGTCACC ATGTCCACCG TCGTCACCGC GAAACTGCAG GAGCAGTTCG ACGCGGGCCT CGAAACCCGT GCCGATTTCA CCATCGACCA GCCGCTCGAA CGCTACGGCC AGGTCGATCA CGCGGTATGG CAGCAGCTCT ATGCGCGCCA GGCGGCGCTG CTGCGCGGGC GCGCATGCGA CGCGTTCATC GGCGGGCTCG GGAAGATCCG CTTACCGGCC GACCGCGTGC CGTCGTTCGC GGACGTGAAC CGCCAGCTGA AGCCGGCCAC CGGCTGGGAG ATCGTCGCGG TGCCGGGCTT GGTGCCCGAT CGCGTGTTCT TCGAGCATCT CGCGCACCGG CGCTTTCCGG TCACCTGGTG GATGCGCCGC CCCGACCAGC TCGACTATCT GCAGGAGCCC GACTGCTTCC ACGACCTGTT CGGCCACGTG CCGCTGCTGA TCGAGCCGGT GTTCGCCGAC TACATGCAGG CCTACGGGCG TACCGCGCTC GCGGTCGCGG ACGACGACGC GGCGCTCGCA CGGCTCGCGC GTCTCTATTG GTATACGGTG GAGTTCGGGC TGATCCGCGA TCCGCGCGGC ACCAACGGGC TGTCGATCTA CGGCGCGGGA ATCGTGTCGA GCAAGGGCGA GAGCCTCTAC AGCGTCGAGA GCGCGGCGCC GAACCGGCTC GGCTTCGACC TGGAGCGCGT GATGCGCACG AAGTACCGGA TCGACACGTT CCAGAAGACC TACTTCGTGA TCGACGACTT CGCGCAGCTG TTCGCGCTGG CCGACGTCGA CGCGCGTGCG CTGGCCGAGC GGCTCGCCGC GCTGCCCGAG CTGTCCGCCG GCACGGTGCT CGATACCGAC GTCGTGCTTC AGCGCGGCAC CGGCGAAGGC TGGCCCGACG ACGACGACGC A

Details for BuviA.00068.a.B1.GE38092

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME NRRHLCAHHA RAPLYTRMVI HPSMQSVTMS TVVTAKLQEQ FDAGLETRAD FTIDQPLERY GQVDHAVWQQ LYARQAALLR GRACDAFIGG LGKIRLPADR VPSFADVNRQ LKPATGWEIV AVPGLVPDRV FFEHLAHRRF PVTWWMRRPD QLDYLQEPDC FHDLFGHVPL LIEPVFADYM QAYGRTALAV ADDDAALARL ARLYWYTVEF GLIRDPRGTN GLSIYGAGIV SSKGESLYSV ESAAPNRLGF DLERVMRTKY RIDTFQKTYF VIDDFAQLFA LADVDARALA ERLAALPELS AGTVLDTDVV LQRGTGEXGP TTTTHK

Validated NT Sequence

cnnnnannat ttnngtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggag aatcgacgcc atctttgcgc ccatcatgcg cgcgccccgc tctacactcg catggtcatt cacccaagca tgcagagcgt caccatgtcc accgtcgtca ccgcgaaact gcaggagcag ttcgacgcgg gcctcgaaac ccgtgccgat ttcaccatcg accagccgct cgaacgctac ggccaggtcg atcacgcggt atggcagcag ctctatgcgc gccaggcggc gctgctgcgc gggcgcgcat gcgacgcgtt catcggcggg ctcgggaaga tccgcttacc ggccgaccgc gtgccgtcgt tcgcggacgt gaaccgccag ctgaagccgg ccaccggctg ggagatcgtc gcggtgccgg gcttggtgcc cgatcgcgtg ttcttcgagc atctcgcgca ccggcgcttt ccggtcacct ggtggatgcg ccgccccgac cagctcgact atctgcagga gcccgactgc ttccacgacc tgttcggcca cgtgccgctg ctgatcgagc cggtgttcgc cgactacatg caggcctacg ggcgtaccgc gctcgcggtc gcggacgacg acgcggcgct cgcacggctc gcgcgtctct attggtatac ggtggagttc gggctgatcc gcgatccgcg cggcaccaac gggctgtcga tctacggcgc gggaatcgtg tcgagcaagg gcgagagcct ctacagcgtc gagagcgcgg cgccgaaccg gctcggcttc gacctggagc gcgtgatgcg cacgaagtac cggatcgaca cgttccagaa gacctacttc gtgatcgacg acttcgcgca gctgttcgcg ctggccgacg tcgacgcgcg tgcgctggcc gagcggctcg ccgcgctgcc cgagctgtcc gccggcacgg tgctcgatac cgacgtcgtg cttcagcgcg gcaccggcga anctggcccg acgacgacga cgcataagtg agtaagatag gatccgntgt acaaaaancc cgcaanna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaga atcgacgcca tctttgcgcc catcatgcgc gcgccccgct ctacactcgc atggtcattc acccaagcat gcagagcgtc accatgtcca ccgtcgtcac cgcgaaactg caggagcagt tcgacgcggg cctcgaaacc cgtgccgatt tcaccatcga ccagccgctc gaacgctacg gccaggtcga tcacgcggta tggcagcagc tctatgcgcg ccaggcggcg ctgctgcgcg ggcgcgcatg cgacgcgttc atcggcgggc tcgggaagat ccgcttaccg gccgaccgcg tgccgtcgtt cgcggacgtg aaccgccagc tgaagccggc caccggctgg gagatcgtcg cggtgccggg cttggtgccc gatcgcgtgt tcttcgagca tctcgcgcac cggcgctttc cggtcacctg gtggatgcgc cgccccgacc agctcgacta tctgcaggag cccgactgct tccacgacct gttcggccac gtgccgctgc tgatcgagcc ggtgttcgcc gactacatgc aggcctacgg gcgtaccgcg ctcgcggtcg cggacgacga cgcggcgctc gcacggctcg cgcgtctcta ttggtatacg gtggagttcg ggctgatccg cgatccgcgc ggcaccaacg ggctgtcgat ctacggcgcg ggaatcgtgt cgagcaaggg cgagagcctc tacagcgtcg agagcgcggc gccgaaccgg ctcggcttcg acctggagcg cgtgatgcgc acgaagtacc ggatcgacac gttccagaag acctacttcg tgatcgacga cttcgcgcag ctgttcgcgc tggccgacgt cgacgcgcgt gcgctggccg agcggctcgc cgcgctgccc gagctgtccg ccggcacggt gctcgatacc gacgtcgtgc ttcagcgcgg caccggcgaa ggctggcccg acgacgacga cgcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat