BuviA.00061.c
UDP-N-acetylglucosamine 2-epimerase
| CENTER ID: | BuviA.00061.c |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00061.c.B1.GE38090 | Full length( BuviA.00061.c ) | 1 | 405 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5DLD
DEPOSITED: 9/4/2015
DETERMINATION: XRay
CLONE: BuviA.00061.c.B1.GE38090
PROTEIN: BuviA.00061.c.B1.PS02389
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131791 |
| PATRIC ID: | fig|269482.11.peg.2881 |
| RefSeq: | YP_001116587.1 |
| UniProt: | A4JLG9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKILLVFGT RPEAIKMAPL VRALKARADV DAKVCVTAQH RQMLDQVLSL FDIEPDYDLD LMRQGQTLSD VTTGILHAIG AVFDDLRPDI VLVHGDTTTT LAVSLAAFYR YLPIGHVEAG LRSGDIWSPW PEELNRRVTD AVSSWHFAPT GQARDNLLSE GVPVGSVVMT GNTVIDALHD VKHMLERDDA LSREVAARFP FLDPTARMVL ITGHRRESFG EPFRNFCEAL RTLAHRYRDA QFVYPLHLNP NVREPALALL GGEANIYLIE PQEYLAFVYL MSRSHFIITD SGGIQEEGPA LGKPVLVTRD TTERPEAIRA GTARLVGTDP ELIVREASRL FDSASAYDEM ARASNPYGDG HASERIVHAL MHTPASSANT TSFSMGAAEL PLNALTLGLQ ALRSP
NT Sequence
ATGAAGAAGA TCTTGCTTGT ATTCGGAACC CGGCCGGAAG CCATCAAGAT GGCGCCGCTC GTTCGCGCGC TGAAGGCGCG CGCGGATGTC GACGCGAAGG TGTGCGTCAC CGCACAACAT CGGCAGATGC TGGATCAGGT GCTGTCGTTG TTCGACATCG AGCCCGACTA CGATCTCGAT CTGATGCGTC AGGGCCAGAC GCTCAGCGAC GTGACGACCG GTATCCTGCA TGCGATCGGC GCGGTGTTCG ACGACCTGCG CCCGGACATC GTCCTCGTGC ATGGCGACAC GACCACCACG CTGGCCGTGA GCCTCGCGGC GTTCTATCGC TATCTGCCGA TCGGGCACGT GGAAGCGGGC CTGCGCAGCG GCGACATCTG GTCGCCGTGG CCGGAGGAGC TGAACCGTCG CGTGACGGAT GCGGTGTCGT CATGGCACTT CGCGCCGACC GGCCAGGCGC GCGACAACCT GTTGAGCGAA GGCGTGCCGG TCGGCTCGGT GGTGATGACG GGCAACACGG TGATCGACGC GCTGCACGAC GTCAAGCACA TGCTCGAACG CGACGACGCG CTCAGCCGCG AGGTCGCCGC GCGCTTTCCG TTCCTCGACC CGACGGCCCG CATGGTGCTG ATCACCGGCC ATCGCCGCGA GAGTTTCGGC GAGCCGTTCC GGAATTTCTG CGAGGCACTG CGCACGCTCG CGCACCGCTA TCGCGACGCG CAGTTCGTCT ACCCGCTGCA CCTGAATCCG AACGTGCGCG AGCCGGCGCT CGCGCTGCTC GGCGGCGAGG CCAACATCTA CCTGATCGAA CCGCAGGAAT ATCTCGCGTT CGTGTATCTG ATGTCGCGTT CGCACTTCAT CATCACGGAC TCGGGCGGCA TCCAGGAGGA AGGGCCGGCA CTCGGCAAGC CCGTGCTCGT CACGCGCGAT ACGACCGAGC GCCCCGAAGC GATCCGGGCC GGCACCGCGC GGCTCGTCGG CACGGACCCG GAGCTCATCG TGCGGGAAGC GTCGCGGCTG TTCGACAGCG CGAGCGCCTA CGACGAGATG GCGCGTGCGA GCAACCCATA CGGCGACGGA CATGCGAGCG AGCGGATCGT CCATGCGCTG ATGCATACGC CGGCGTCGAG CGCGAATACG ACGAGCTTCT CGATGGGCGC GGCCGAACTG CCGCTCAACG CGCTCACGCT CGGATTGCAG GCGTTGCGCT CGCCC
Details for BuviA.00061.c.B1.GE38090
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 41kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 69 |
Validated AA Sequence
MAHHHHHHMK KILLVFGTRP EAIKMAPLVR ALKARADVDA KVCVTAQHRQ MLDQVLSLFD IEPDYDLDLM RQGQTLSDVT TGILHAIGAV FDDLRPDIVL VHGDTTTTLA VSLAAFYRYL PIGHVEAGLR SGDIWSPWPE ELNRRVTDAV SSWHFAPTGQ ARDNLLSEGV PVGSVVMTGN TVIDALHDVK HMLERDDALS REVAARFPFL DPTARMVLIT GHRRESFGEP FRNFCEALRT LAHRYRDAQF VYPLHLNPNV REPALALLGG EANIYLIEPQ NISRSCI
Validated NT Sequence
tttntnanct ttttnanaag gagatatacc atggctcacc accaccacca ccatatgaag aagatcttgc ttgtattcgg aacccggccg gaagccatca agatggcgcc gctcgttcgc gcgctgaagg cgcgcgcgga tgtcgacgcg aaggtgtgcg tcaccgcaca acatcggcag atgctggatc aggtgctgtc gttgttcgac atcgagcccg actacgatct cgatctgatg cgtcagggcc agacgctcag cgacgtgacg accggtatcc tgcatgcgat cggcgcggtg ttcgacgacc tgcgcccgga catcgtcctc gtgcatggcg acacgaccac cacgctggcc gtgagcctcg cggcgttcta tcgctatctg ccgatcgggc acgtggaagc gggcctgcgc agcggcgaca tctggtcgcc gtggccggag gagctgaacc gtcgcgtgac ggatgcggtg tcgtcatggc acttcgcgcc gaccggccag gcgcgcgaca acctgttgag cgaaggcgtg ccggtcggct cggtggtgat gacgggcaac acggtgatcg acgcgctgca cgacgtcaag cacatgctcg aacgcgacga cgcgctcagc cgcgaggtcg ccgcgcgctt tccgttcctc gacccgacgg cccgcatggt gctgatcacc ggccatcgcc gcgagagttt cggcgagccg ttccggaatt tctgcgaggc actgcgcacg ctcgcgcacc gctatcgcga cgcgcagttc gtctacccgc tgcacctgaa tccgaacgtg cgcgagccgg cgctcgcgct gctcggcggc gaggccaaca tctacctgat cgaaccgcag aatatctcgc gttcgtgtat ctgatgtcgc gttcgcactt catcatcacg gactcgggcg gcatccagga ggaaggncgg nactcggcag cccgtgctcg tcacgcgcga tacgaccgag cgccccgaag cgatccgggc cggcaccnnn nngctcgtcg gcacggaccc gganctcatc gtgcggancg tcgcngcttn tnnnnnnnnn gagcncctac gacnngatgg cncnntggnn agccacccat acggnnacng acatgntcgt cntgnnntga tgcntnnncn gnnngagcnn nnnnnnnngn tntcnngggn cgggcnnnnn ncnntnangg nnnacnnnnn nntgcnngnn nnggcnnnnc ctnnnntnat annaantgnn nnggngnnng ntnnnnnna
Expected Protein Sequence
MAHHHHHHMK KILLVFGTRP EAIKMAPLVR ALKARADVDA KVCVTAQHRQ MLDQVLSLFD IEPDYDLDLM RQGQTLSDVT TGILHAIGAV FDDLRPDIVL VHGDTTTTLA VSLAAFYRYL PIGHVEAGLR SGDIWSPWPE ELNRRVTDAV SSWHFAPTGQ ARDNLLSEGV PVGSVVMTGN TVIDALHDVK HMLERDDALS REVAARFPFL DPTARMVLIT GHRRESFGEP FRNFCEALRT LAHRYRDAQF VYPLHLNPNV REPALALLGG EANIYLIEPQ EYLAFVYLMS RSHFIITDSG GIQEEGPALG KPVLVTRDTT ERPEAIRAGT ARLVGTDPEL IVREASRLFD SASAYDEMAR ASNPYGDGHA SERIVHALMH TPASSANTTS FSMGAAELPL NALTLGLQAL RSP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga agatcttgct tgtattcgga acccggccgg aagccatcaa gatggcgccg ctcgttcgcg cgctgaaggc gcgcgcggat gtcgacgcga aggtgtgcgt caccgcacaa catcggcaga tgctggatca ggtgctgtcg ttgttcgaca tcgagcccga ctacgatctc gatctgatgc gtcagggcca gacgctcagc gacgtgacga ccggtatcct gcatgcgatc ggcgcggtgt tcgacgacct gcgcccggac atcgtcctcg tgcatggcga cacgaccacc acgctggccg tgagcctcgc ggcgttctat cgctatctgc cgatcgggca cgtggaagcg ggcctgcgca gcggcgacat ctggtcgccg tggccggagg agctgaaccg tcgcgtgacg gatgcggtgt cgtcatggca cttcgcgccg accggccagg cgcgcgacaa cctgttgagc gaaggcgtgc cggtcggctc ggtggtgatg acgggcaaca cggtgatcga cgcgctgcac gacgtcaagc acatgctcga acgcgacgac gcgctcagcc gcgaggtcgc cgcgcgcttt ccgttcctcg acccgacggc ccgcatggtg ctgatcaccg gccatcgccg cgagagtttc ggcgagccgt tccggaattt ctgcgaggca ctgcgcacgc tcgcgcaccg ctatcgcgac gcgcagttcg tctacccgct gcacctgaat ccgaacgtgc gcgagccggc gctcgcgctg ctcggcggcg aggccaacat ctacctgatc gaaccgcagg aatatctcgc gttcgtgtat ctgatgtcgc gttcgcactt catcatcacg gactcgggcg gcatccagga ggaagggccg gcactcggca agcccgtgct cgtcacgcgc gatacgaccg agcgccccga agcgatccgg gccggcaccg cgcggctcgt cggcacggac ccggagctca tcgtgcggga agcgtcgcgg ctgttcgaca gcgcgagcgc ctacgacgag atggcgcgtg cgagcaaccc atacggcgac ggacatgcga gcgagcggat cgtccatgcg ctgatgcata cgccggcgtc gagcgcgaat acgacgagct tctcgatggg cgcggccgaa ctgccgctca acgcgctcac gctcggattg caggcgttgc gctcgccctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat