BuviA.00061.a

UDP-N-acetylglucosamine 2-epimerase

CENTER ID: BuviA.00061.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00061.a.B1.GE38088 Full length( BuviA.00061.a ) 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.5028
RefSeq: YP_001118657.1
UniProt: A4JC19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRTILCVVG TRPEAIKMAP VILRLRADPG FKVHVLATAQ HRDLLDQILT CFDIKPDVDL DIMRPNQGLA SLTSRLLDGI DAVLVDKRPD LVLGQGDTTT VMATALACFY RHIPFGHIEA GLRTRDLRNP FPEEMNRVFA GRLARLHFAP TASSRDNLLH ENIPSDQIWV TGNTVIDALH TVLRTHPSCG VEIDDTKRLI LVTAHRRENF GAPLERICHA IRQIVDANDD VEVLFPVHPN PHVQRTTREL LSHHARIRLC SPLDYVPFVA AMQRSYLILS DSGGVQEEAP ALGKPVLVLR DETERPEAVE AGVVKLVGTQ TDTIVDAVQL MLDNADEYSK VARGVSPYGD GKAAHRIFDV IRKQCV
NT Sequence
ATGTCTCGAA CCATACTGTG CGTCGTAGGC ACGCGACCGG AGGCCATCAA GATGGCGCCG GTCATCCTGC GTTTGCGAGC GGATCCGGGA TTCAAGGTGC ACGTGCTCGC GACCGCGCAA CATCGCGATC TGCTCGACCA GATCCTGACC TGTTTCGATA TCAAACCGGA TGTCGATCTC GACATCATGC GTCCGAATCA GGGGCTTGCC AGCCTGACGT CGCGTCTGCT TGACGGCATC GATGCCGTGC TCGTGGACAA GCGTCCCGAT CTCGTACTGG GACAAGGCGA CACCACGACG GTTATGGCCA CGGCGCTTGC ATGCTTCTAT CGGCATATAC CGTTCGGTCA TATTGAAGCG GGCCTGCGTA CCCGCGACCT CCGCAACCCG TTTCCTGAAG AGATGAACCG CGTTTTCGCG GGGCGCCTTG CAAGGCTGCA TTTCGCGCCG ACAGCGAGTT CGCGCGACAA CCTCTTGCAT GAAAACATCC CCAGCGACCA GATCTGGGTG ACCGGGAACA CCGTCATCGA CGCGCTTCAC ACTGTCTTGC GGACACATCC GTCCTGCGGC GTCGAGATCG ACGACACGAA ACGGCTGATT CTAGTCACCG CGCATCGCCG GGAGAATTTT GGCGCCCCGC TCGAACGGAT CTGCCATGCC ATTCGCCAGA TTGTCGACGC CAACGACGAC GTCGAGGTGC TGTTTCCCGT CCATCCCAAT CCGCACGTCC AGCGTACGAC GCGCGAACTG CTGTCGCACC ACGCCCGGAT CCGACTCTGC TCCCCGCTCG ACTATGTGCC GTTCGTCGCC GCCATGCAGC GCTCGTACCT CATCCTCAGC GACTCGGGTG GCGTGCAGGA AGAAGCGCCC GCTCTGGGCA AACCGGTCCT GGTGTTGCGC GACGAGACCG AGCGCCCGGA AGCTGTCGAA GCTGGTGTCG TCAAGCTCGT CGGCACTCAA ACCGATACGA TCGTCGATGC AGTCCAGCTG ATGCTCGACA ACGCCGACGA ATACAGCAAG GTTGCACGCG GCGTCTCGCC CTACGGTGAC GGAAAGGCGG CTCACCGGAT TTTCGACGTC ATCCGGAAAC AATGCGTG
Details for BuviA.00061.a.B1.GE38088
HARVESTED ON: 5/13/2014
SEQUENCED ON: 5/6/2014
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MAPVILRLRA DPGFKVHVLA TAQHRDLLDQ ILTCFDIKPD VDLDIMRPNQ GLASLTSRLL DGIDAVLVDK RPDLVLGQGD TTTVMATALA CFYRHIPFGH IEAGLRTRDL RNPFPEEMNR VFAGRLARLH FAPTASSRDN LLHENIPSDQ IWVTGNTVID ALHTVLRTHP SCGVEIDDTK RLILVTAHRR ENFGAPLERI CHAIRQIVDA NDDVEVLFPV HPNPHVQRTT RELLSHHARI RLCSPLDYVP FVAAMQRSYL ILSDSGGVQE EAPALGKPVL VLRDETERPE AVEAGVVKLV GTQTDTIVDA VQLMLDNADE YSKVARGVSP YGDGKAAHRI FDV
Validated NT Sequence
gatgacgtcg aaaatccggt gagccgcctt tccgtcaccg tagggcgaga cgccgcgtgc aaccttgctg tattcgtcgg cgttgtcgag catcagctgg actgcatcga cgatcgtatc ggtttgagtg ccgacgagct tgacgacacc agcttcgaca gcttccgggc gctcggtctc gtcgcgcaac accaggaccg gtttgcccag agcgggcgct tcttcctgca cgccacccga gtcgctgagg atgaggtacg agcgctgcat ggcggcgacg aacggcacat agtcgagcgg ggagcagagt cggatccggg cgtggtgcga cagcagttcg cgcgtcgtac gctggacgtg cggattggga tggacgggaa acagcacctc gacgtcgtcg ttggcgtcga caatctggcg aatggcatgg cagatccgtt cgagcggggc gccaaaattc tcccggcgat gcgcggtgac tagaatcagc cgtttcgtgt cgtcgatctc gacgccgcag gacggatgtg tccgcaagac agtgtgaagc gcgtcgatga cggtgttccc ggtcacccag atctggtcgc tggggatgtt ttcatgcaag aggttgtcgc gcgaactcgc tgtcggcgcg aaatgcagcc ttgcaaggcg ccccgcgaaa acgcggttca tctcttcagg aaacgggttg cggaggtcgc gggtacgcag gcccgcttca atatgaccga acggtatatg ccgatagaag catgcaagcg ccgtggccat aaccgtcgtg gtgtcgcctt gtcccagtac gagatcggga cgcttgtcca cgagcacggc atcgatgccg tcaagcagac gcgacgtcag gctggcaagc ccctgattcg gacgcatgat gtcgagatcg acatccggtt tgatatcgaa acaggtcagg atctggtcga gcagatcgcg atgttgcgcg gtcgcgagca cgtgcacctt gaatcccgga tccgctcgca aacgcaggat gaccggcgcc atcttgatgg cctccggtcg cgtgcctacg acgcacagta tgg
Expected Protein Sequence
MAHHHHHHMS RTILCVVGTR PEAIKMAPVI LRLRADPGFK VHVLATAQHR DLLDQILTCF DIKPDVDLDI MRPNQGLASL TSRLLDGIDA VLVDKRPDLV LGQGDTTTVM ATALACFYRH IPFGHIEAGL RTRDLRNPFP EEMNRVFAGR LARLHFAPTA SSRDNLLHEN IPSDQIWVTG NTVIDALHTV LRTHPSCGVE IDDTKRLILV TAHRRENFGA PLERICHAIR QIVDANDDVE VLFPVHPNPH VQRTTRELLS HHARIRLCSP LDYVPFVAAM QRSYLILSDS GGVQEEAPAL GKPVLVLRDE TERPEAVEAG VVKLVGTQTD TIVDAVQLML DNADEYSKVA RGVSPYGDGK AAHRIFDVIR KQCV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc gaaccatact gtgcgtcgta ggcacgcgac cggaggccat caagatggcg ccggtcatcc tgcgtttgcg agcggatccg ggattcaagg tgcacgtgct cgcgaccgcg caacatcgcg atctgctcga ccagatcctg acctgtttcg atatcaaacc ggatgtcgat ctcgacatca tgcgtccgaa tcaggggctt gccagcctga cgtcgcgtct gcttgacggc atcgatgccg tgctcgtgga caagcgtccc gatctcgtac tgggacaagg cgacaccacg acggttatgg ccacggcgct tgcatgcttc tatcggcata taccgttcgg tcatattgaa gcgggcctgc gtacccgcga cctccgcaac ccgtttcctg aagagatgaa ccgcgttttc gcggggcgcc ttgcaaggct gcatttcgcg ccgacagcga gttcgcgcga caacctcttg catgaaaaca tccccagcga ccagatctgg gtgaccggga acaccgtcat cgacgcgctt cacactgtct tgcggacaca tccgtcctgc ggcgtcgaga tcgacgacac gaaacggctg attctagtca ccgcgcatcg ccgggagaat tttggcgccc cgctcgaacg gatctgccat gccattcgcc agattgtcga cgccaacgac gacgtcgagg tgctgtttcc cgtccatccc aatccgcacg tccagcgtac gacgcgcgaa ctgctgtcgc accacgcccg gatccgactc tgctccccgc tcgactatgt gccgttcgtc gccgccatgc agcgctcgta cctcatcctc agcgactcgg gtggcgtgca ggaagaagcg cccgctctgg gcaaaccggt cctggtgttg cgcgacgaga ccgagcgccc ggaagctgtc gaagctggtg tcgtcaagct cgtcggcact caaaccgata cgatcgtcga tgcagtccag ctgatgctcg acaacgccga cgaatacagc aaggttgcac gcggcgtctc gccctacggt gacggaaagg cggctcaccg gattttcgac gtcatccgga aacaatgcgt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat