SSGCID: Target Page

BuviA.00057.t

ABC transporter related

CENTER ID:	BuviA.00057.t
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00057.t.B1.GE35361	Full length( BuviA.00057.t )		1	247

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.222
RefSeq:	YP_001109828.1
UniProt:	A4JUU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSNTDFLLAI EDLTVSFDGF KAIDSLNLYL ERGELRVVIG PNGAGKTTLL DLICGKTKET AGSIKFRNEE ITRLAEFRRV RQGIGRKFQT PSIYENLSVA QNLEVSFPRG RSVFGALAYR EDTEMADRVR DVANEIGLAA TLDMEAGLLS HGQKQWLEIG MLLMQDPELL MLDEPIAGMS VRERELTAEL LDRICRNRSM IVIEHDMAFV ERIAHKVTVM HQGKILAEGP MAQVQADPRV IDVYLGH

NT Sequence

ATGAGCAATA CCGACTTTCT GCTCGCGATC GAAGACCTGA CCGTGTCGTT CGACGGCTTC AAGGCGATCG ATTCGCTCAA TCTCTATCTC GAACGCGGCG AGCTCCGGGT GGTGATCGGC CCGAACGGCG CGGGCAAGAC CACGCTGCTC GATCTCATCT GCGGCAAAAC GAAGGAAACG GCGGGCAGCA TCAAGTTCCG CAACGAGGAA ATCACGCGTC TCGCGGAATT CCGTCGCGTG CGTCAAGGAA TTGGCCGCAA GTTCCAGACG CCGTCGATTT ACGAAAACCT GAGTGTGGCG CAGAACCTCG AAGTGTCGTT TCCACGCGGA CGCAGCGTGT TTGGCGCGCT CGCCTATCGA GAGGATACCG AAATGGCCGA TCGCGTGCGC GACGTGGCCA ATGAAATCGG CCTCGCCGCC ACGCTCGATA TGGAAGCCGG GTTGCTTTCG CACGGCCAGA AACAGTGGCT CGAAATCGGC ATGCTGCTCA TGCAGGATCC CGAACTGCTG ATGCTAGACG AACCGATCGC GGGCATGAGC GTGCGCGAGC GCGAACTGAC GGCCGAGCTG CTCGACCGCA TCTGCCGCAA TCGCTCGATG ATCGTGATCG AACACGACAT GGCTTTCGTG GAGCGCATCG CGCACAAGGT CACGGTCATG CATCAGGGAA AGATTCTCGC GGAAGGCCCG ATGGCGCAGG TGCAAGCCGA CCCGCGCGTG ATCGACGTCT ACCTGGGCCA C

Details for BuviA.00057.t.B1.GE35361

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS NTDFLLAIED LTVSFDGFKA IDSLNLYLER GELRVVIGPN GAGKTTLLDL ICGKTKETAG SIKFRNEEIT RLAEFRRVRQ GIGRKFQTPS IYENLSVAQN LEVSFPRGRS VFGALAYRED TEMADRVRDV ANEIGLAATL DMEAGLLSHG QKQWLEIGML LMQDPELLML DEPIAGMSVR ERELTAELLD RICRNRSMIV IEHDMAFVER IAHKVTVMHQ GKILAEGPMA QVQADPRVID VYLGH

Validated NT Sequence

attttnttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgagc aataccgact ttctgctcgc gatcgaagac ctgaccgtgt cgttcgacgg cttcaaggcg atcgattcgc tcaatctcta tctcgaacgc ggcgagctcc gggtggtgat cggcccgaac ggcgcgggca agaccacgct gctcgatctc atctgcggca aaacgaagga aacggcgggc agcatcaagt tccgcaacga ggaaatcacg cgtctcgcgg aattccgtcg cgtgcgtcaa ggaattggcc gcaagttcca gacgccgtcg atttacgaaa acctgagtgt ggcgcagaac ctcgaagtgt cgtttccacg cggacgcagc gtgtttggcg cgctcgccta tcgagaggat accgaaatgg ccgatcgcgt gcgcgacgtg gccaatgaaa tcggcctcgc cgccacgctc gatatggaag ccgggttgct ttcgcacggc cagaaacagt ggctcgaaat cggcatgctg ctcatgcagg atcccgaact gctgatgcta gacgaaccga tcgcgggcat gagcgtgcgc gagcgcgaac tgacggccga gctgctcgac cgcatctgcc gcaatcgctc gatgatcgtg atcgaacacg acatggcttt cgtggagcgc atcgcgcaca aggtcacggt catgcatcag ggaaagattc tcgcggaagg cccgatggcg caggtgcaag ccgacccgcg cgtgatcgac gtctacctgg gccactaagt gagtaagata ggatccggct gcta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ataccgactt tctgctcgcg atcgaagacc tgaccgtgtc gttcgacggc ttcaaggcga tcgattcgct caatctctat ctcgaacgcg gcgagctccg ggtggtgatc ggcccgaacg gcgcgggcaa gaccacgctg ctcgatctca tctgcggcaa aacgaaggaa acggcgggca gcatcaagtt ccgcaacgag gaaatcacgc gtctcgcgga attccgtcgc gtgcgtcaag gaattggccg caagttccag acgccgtcga tttacgaaaa cctgagtgtg gcgcagaacc tcgaagtgtc gtttccacgc ggacgcagcg tgtttggcgc gctcgcctat cgagaggata ccgaaatggc cgatcgcgtg cgcgacgtgg ccaatgaaat cggcctcgcc gccacgctcg atatggaagc cgggttgctt tcgcacggcc agaaacagtg gctcgaaatc ggcatgctgc tcatgcagga tcccgaactg ctgatgctag acgaaccgat cgcgggcatg agcgtgcgcg agcgcgaact gacggccgag ctgctcgacc gcatctgccg caatcgctcg atgatcgtga tcgaacacga catggctttc gtggagcgca tcgcgcacaa ggtcacggtc atgcatcagg gaaagattct cgcggaaggc ccgatggcgc aggtgcaagc cgacccgcgc gtgatcgacg tctacctggg ccactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat