SSGCID: Target Page

BuviA.00057.q

ABC transporter related

CENTER ID:	BuviA.00057.q
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00057.q.B1.GE35358	Full length( BuviA.00057.q )		1	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.1173
RefSeq:	YP_001114959.1
UniProt:	A4JR01

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLHIEKLSAG YGDSAVLHRI DLEVGPNEVV GVLGCNGAGK TTLVKAIMGL LPCVSGNVVF QGQSITGLRT HAIARRGIGL VPQGRWIFPK LTVRENLIMG TQAAGGGAIL DEVFEYFPIL KSRLSQNGGT LSGGEQQVLA IARALCGRPK LLLLDEPSDG VQPNMVELIG EVIPTLCRRS QLAVLLVEQN LDLVLRSAQR CIVLKNGRLA HTGHVEPRSG AAGAHPLAQY LSPAAESATS SVSSPHSTHS NRTSTQ

NT Sequence

ATGTTGCACA TTGAAAAGCT ATCTGCGGGC TACGGCGACT CCGCGGTGCT GCACCGGATC GACCTCGAGG TTGGACCCAA CGAAGTGGTC GGCGTGCTTG GCTGCAATGG CGCGGGCAAG ACGACGCTGG TCAAGGCGAT CATGGGACTG CTGCCGTGCG TAAGTGGCAA CGTCGTGTTT CAGGGGCAGT CCATCACCGG CCTGCGTACC CATGCGATTG CCCGGCGCGG CATTGGACTG GTACCGCAGG GGCGCTGGAT TTTTCCGAAA CTGACTGTGC GCGAGAACCT CATCATGGGC ACGCAGGCCG CGGGCGGGGG GGCGATCCTT GATGAGGTGT TCGAGTATTT CCCTATTCTC AAGTCCAGAC TCTCGCAGAA CGGCGGCACG CTTTCGGGTG GCGAACAGCA GGTGTTGGCG ATCGCCCGTG CGCTGTGCGG CCGGCCGAAG CTGCTGTTGC TGGACGAACC GTCCGACGGT GTGCAGCCGA ATATGGTCGA ACTGATCGGC GAGGTTATTC CCACCCTGTG CCGCCGCTCG CAGCTCGCCG TGCTCCTGGT CGAGCAGAAC CTCGATCTCG TGCTGCGCAG CGCACAGCGC TGCATCGTGC TCAAGAACGG GCGACTTGCC CATACCGGCC ACGTCGAGCC GCGAAGCGGT GCCGCGGGGG CTCATCCGTT GGCGCAATAC CTGTCGCCGG CCGCCGAGTC TGCCACGTCT TCAGTCTCGT CACCACATTC AACGCATTCC AACCGGACTT CCACCCAA

Details for BuviA.00057.q.B1.GE35358

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML HIEKLSAGYG DSAVLHRIDL EVGPNEVVGV LGCNGAGKTT LVKAIMGLLP CVSGNVVFQG QSITGLRTHA IARRGIGLVP QGRWIFPKLT VRENLIMGTQ AAGGGAILDE VFEYFPILKS RLSQNGGTLS GGEQQVLAIA RALCGRPKLL LLDEPSDGVQ PNMVELIGEV IPTLCRRSQL AVLLVEQNLD LVLRSAQRCI VLKNGRLAHT GHVEPRSGAA GAHPLAQYLS PAAESATSSV SSPHSTHSXR TSTQ

Validated NT Sequence

aattttgtnn nnnttaagaa ggagatatac catggctcac caccaccacc accatatgtt gcacattgaa aagctatctg cgggctacgg cgactccgcg gtgctgcacc ggatcgacct cgaggttgga cccaacgaag tggtcggcgt gcttggctgc aatggcgcgg gcaagacgac gctggtcaag gcgatcatgg gactgctgcc gtgcgtaagt ggcaacgtcg tgtttcaggg gcagtccatc accggcctgc gtacccatgc gattgcccgg cgcggcattg gactggtacc gcaggggcgc tggatttttc cgaaactgac tgtgcgcgag aacctcatca tgggcacgca ggccgcgggc gggggggcga tccttgatga ggtgttcgag tatttcccta ttctcaagtc cagactctcg cagaacggcg gcacgctttc gggtggcgaa cagcaggtgt tggcgatcgc ccgtgcgctg tgcggccggc cgaagctgct gttgctggac gaaccgtccg acggtgtgca gccgaatatg gtcgaactga tcggcgaggt tattcccacc ctgtgccgcc gctcgcagct cgccgtgctc ctggtcgagc agaacctcga tctcgtgctg cgcagcgcac agcgctgcat cgtgctcaag aacgggcgac ttgcccatac cggccacgtc gagccgcgaa gcggtgccgc gggggctcat ccgttggcgc aatacctgtc gccggccgcc gagtctgcca cgtcttcagt ctcgtcacca cattcaacgc attccnaccg gacttccacc caataagtga gtaagatagg atccggctgc taacaannnc ccgaang

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc acattgaaaa gctatctgcg ggctacggcg actccgcggt gctgcaccgg atcgacctcg aggttggacc caacgaagtg gtcggcgtgc ttggctgcaa tggcgcgggc aagacgacgc tggtcaaggc gatcatggga ctgctgccgt gcgtaagtgg caacgtcgtg tttcaggggc agtccatcac cggcctgcgt acccatgcga ttgcccggcg cggcattgga ctggtaccgc aggggcgctg gatttttccg aaactgactg tgcgcgagaa cctcatcatg ggcacgcagg ccgcgggcgg gggggcgatc cttgatgagg tgttcgagta tttccctatt ctcaagtcca gactctcgca gaacggcggc acgctttcgg gtggcgaaca gcaggtgttg gcgatcgccc gtgcgctgtg cggccggccg aagctgctgt tgctggacga accgtccgac ggtgtgcagc cgaatatggt cgaactgatc ggcgaggtta ttcccaccct gtgccgccgc tcgcagctcg ccgtgctcct ggtcgagcag aacctcgatc tcgtgctgcg cagcgcacag cgctgcatcg tgctcaagaa cgggcgactt gcccataccg gccacgtcga gccgcgaagc ggtgccgcgg gggctcatcc gttggcgcaa tacctgtcgc cggccgccga gtctgccacg tcttcagtct cgtcaccaca ttcaacgcat tccaaccgga cttccaccca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat