SSGCID: Target Page

BuviA.00057.o

ABC transporter related

CENTER ID:	BuviA.00057.o
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00057.o.B1.GE35982	Full length( BuviA.00057.o )		1	238

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.3068
RefSeq:	YP_001116769.1
UniProt:	A4JM01

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MATPMLKIKG LHVNYGGIQA VKGVDLEIQK GELVTLIGAN GAGKTTTMKA ITGLKPYSAG DIEYMGQSIK DVPAHELLKR GLAMVPEGRG IFARMSIVEN MQMGAYLRSD TDGIKKDVER MFGFFPRLKE RATQLAGTLS GGEQQMLAMA RAVISKPKLL LLDEPSMGLS PIMVEKIFEV VREISKEGIT LMLVEQNARL ALQAADRGYV MDSGAVTMSG DAKQMLDDPE VRAAYLGE

NT Sequence

ATGGCTACGC CAATGCTGAA AATCAAAGGC CTTCACGTCA ACTATGGCGG CATTCAGGCC GTCAAGGGTG TCGACCTCGA GATCCAGAAG GGGGAACTGG TCACGCTGAT TGGCGCCAAT GGCGCGGGCA AGACGACGAC GATGAAGGCG ATTACAGGTC TGAAGCCGTA TTCGGCGGGC GACATCGAAT ACATGGGCCA GTCGATTAAG GACGTACCGG CGCATGAGTT GCTGAAGCGT GGTCTCGCGA TGGTGCCGGA AGGGCGCGGT ATTTTTGCGC GGATGTCGAT TGTTGAGAAC ATGCAGATGG GTGCTTATCT GCGTAGCGAC ACCGATGGCA TCAAGAAGGA TGTCGAGCGG ATGTTCGGGT TCTTTCCGCG GCTGAAGGAA CGTGCTACGC AGCTCGCGGG CACGCTTTCG GGCGGCGAAC AGCAGATGTT GGCGATGGCG CGTGCCGTGA TTTCCAAGCC GAAGCTGCTG CTGCTCGACG AGCCGTCGAT GGGCCTGTCA CCGATCATGG TCGAGAAGAT CTTCGAAGTG GTGCGCGAGA TTTCCAAGGA AGGCATCACG CTGATGCTGG TCGAGCAGAA CGCGCGTCTC GCGTTGCAAG CTGCCGATCG CGGCTACGTG ATGGACTCGG GCGCGGTCAC GATGTCGGGT GATGCGAAGC AGATGCTTGA CGATCCGGAA GTGCGGGCTG CTTATCTGGG TGAA

Details for BuviA.00057.o.B1.GE35982

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHMAT PMLKIKGLHV NYGGIQAVKG VDLEIQKGEL VTLIGANGAG KTTTMKAITG LKPYSAGDIE YMGQSIKDVP AHELLKRGLA MVPEGRGIFA RMSIVENMQM GAYLRSDTDG IKKDVERMFG FFPRLKERAT QLAGTLSGGE QQMLAMARAV ISKPKLLLLD EPSMGLSPIM VEKIFEVVRE ISKEGITLML VEQNARLALQ AADRGYVMDS GAVTMSGDAK QXLDDPEVRA AYLGE

Validated NT Sequence

attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca tatggctacg ccaatgctga aaatcaaagg ccttcacgtc aactatggcg gcattcaggc cgtcaagggt gtcgacctcg agatccagaa gggggaactg gtcacgctga ttggcgccaa tggcgcgggc aagacgacga cgatgaaggc gattacaggt ctgaagccgt attcggcggg cgacatcgaa tacatgggcc agtcgattaa ggacgtaccg gcgcatgagt tgctgaagcg tggtctcgcg atggtgccgg aagggcgcgg tatttttgcg cggatgtcga ttgttgagaa catgcagatg ggtgcttatc tgcgtagcga caccgatggc atcaagaagg atgtcgagcg gatgttcggg ttctttccgc ggctgaagga acgtgctacg cagctcgcgg gcacgctttc gggcggcgaa cagcagatgt tggcgatggc gcgtgccgtg atttccaagc cgaagctgct gctgctcgac gagccgtcga tgggcctgtc accgatcatg gtcgagaaga tcttcgaagt ggtgcgcgag atttccaagg aaggcatcac gctgatgctg gtcgagcaga acgcgcgtct cgcgttgcaa gctgccgatc gcggctacgt gatggactcg ggcgcggtca cgatgtcggg tgatgcgaag cagangcttg acgatccgga agtgcgggct gcttatctgg gtgaataagt gagtaagata ggatccggct gctacannnc cccgaaangn nnnnnnaaga cnnnnnnnnn nnnnnnnnnn ngngnnaagn annnnnnnnn tcnnntcgnn nntcnngann nnnnnngnnn gnntccgnan anagcnacnn nnntnctgnt nncnacagtc nnnntcgnnn nanaantacn gnnnnntncn gnnnnngacn cnnnntnnnn nnnntnggnn ccggtncntn gggnccnngn nnnnnnntnt tcnncccngn nnnnnanngg g

Expected Protein Sequence

MAHHHHHHMA TPMLKIKGLH VNYGGIQAVK GVDLEIQKGE LVTLIGANGA GKTTTMKAIT GLKPYSAGDI EYMGQSIKDV PAHELLKRGL AMVPEGRGIF ARMSIVENMQ MGAYLRSDTD GIKKDVERMF GFFPRLKERA TQLAGTLSGG EQQMLAMARA VISKPKLLLL DEPSMGLSPI MVEKIFEVVR EISKEGITLM LVEQNARLAL QAADRGYVMD SGAVTMSGDA KQMLDDPEVR AAYLGE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta cgccaatgct gaaaatcaaa ggccttcacg tcaactatgg cggcattcag gccgtcaagg gtgtcgacct cgagatccag aagggggaac tggtcacgct gattggcgcc aatggcgcgg gcaagacgac gacgatgaag gcgattacag gtctgaagcc gtattcggcg ggcgacatcg aatacatggg ccagtcgatt aaggacgtac cggcgcatga gttgctgaag cgtggtctcg cgatggtgcc ggaagggcgc ggtatttttg cgcggatgtc gattgttgag aacatgcaga tgggtgctta tctgcgtagc gacaccgatg gcatcaagaa ggatgtcgag cggatgttcg ggttctttcc gcggctgaag gaacgtgcta cgcagctcgc gggcacgctt tcgggcggcg aacagcagat gttggcgatg gcgcgtgccg tgatttccaa gccgaagctg ctgctgctcg acgagccgtc gatgggcctg tcaccgatca tggtcgagaa gatcttcgaa gtggtgcgcg agatttccaa ggaaggcatc acgctgatgc tggtcgagca gaacgcgcgt ctcgcgttgc aagctgccga tcgcggctac gtgatggact cgggcgcggt cacgatgtcg ggtgatgcga agcagatgct tgacgatccg gaagtgcggg ctgcttatct gggtgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at