SSGCID: Target Page

BuviA.00057.n

ABC transporter related

CENTER ID:	BuviA.00057.n
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00057.n.B1.GE35981	Full length( BuviA.00057.n )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.3067
RefSeq:	YP_001116768.1
UniProt:	A4JM00

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDNKIRLSV KGVNKRFGGL QALLDVGLQI EEGTIYGLIG PNGAGKTTFF NVITGLYTPD SGEFKLDGTP YTPSAVYQVA KAGIARTFQN IRLFGGMTAL ENVMVGRHVR TKHGLVGAVF QTPAERGEER EIKERALELL EYVGVAQYAD YTSRNLSYGH QRRLEIARAL ATDPKLLALD EPAAGMNATE KVELTKLLDK IRADGKTILL IEHDVKLVMG LCDEMTVLDY GKVIAEGLPQ DVQKDPKVIE AYLGAGGH

NT Sequence

ATGAGCGACA ACAAAATCCG TCTGTCCGTA AAAGGCGTGA ACAAGCGCTT TGGTGGCTTG CAGGCGCTGT TGGACGTGGG CCTTCAGATC GAAGAAGGCA CGATTTATGG TCTGATCGGC CCGAACGGCG CCGGCAAGAC CACGTTCTTC AACGTGATTA CGGGGCTGTA CACGCCCGAC TCGGGCGAAT TCAAGCTCGA CGGCACGCCG TACACGCCGA GTGCGGTGTA TCAGGTGGCG AAGGCGGGCA TCGCGCGCAC GTTCCAGAAC ATTCGTCTCT TCGGCGGTAT GACGGCGCTG GAGAACGTGA TGGTCGGACG TCACGTTCGC ACGAAGCACG GTCTGGTCGG CGCGGTGTTC CAGACGCCCG CCGAACGCGG CGAAGAGCGC GAGATCAAGG AGCGAGCGCT GGAACTGCTC GAATACGTCG GCGTGGCGCA GTACGCGGAC TACACGTCGC GCAACCTGTC ATACGGTCAC CAGCGGCGTC TGGAGATCGC ACGTGCTCTA GCGACCGATC CGAAGCTGCT CGCGCTCGAC GAACCGGCTG CCGGCATGAA TGCGACGGAG AAGGTCGAAC TGACGAAGCT GCTCGACAAG ATTCGCGCGG ACGGCAAGAC GATTCTGCTG ATCGAGCACG ACGTGAAACT GGTGATGGGC CTGTGTGACG AGATGACAGT GCTCGACTAT GGCAAGGTGA TTGCCGAGGG ATTGCCGCAG GACGTCCAGA AGGACCCGAA GGTGATCGAG GCTTATCTGG GTGCGGGGGG CCAT

Details for BuviA.00057.n.B1.GE35981

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS DNKIRLSVKG VNKRFGGLQA LLDVGLQIEE GTIYGLIGPN GAGKTTFFNV ITGLYTPDSG EFKLDGTPYT PSAVYQVAKA GIARTFQNIR LFGGMTALEN VMVGRHVRTK HGLVGAVFQT PAERGEEREI KERALELLEY VGVAQYADYT SRNLSYGHQR RLEIARALAT DPKLLALDEP AAGMNATEKV ELTKLLDKIR ADGKTILLIE HDVKLVMGLC DEMTVLDYGK VIAEGLPXDV QKDPKVIEAY LGAGGH

Validated NT Sequence

ttgtttnnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgagcga caacaaaatc cgtctgtccg taaaaggcgt gaacaagcgc tttggtggct tgcaggcgct gttggacgtg ggccttcaga tcgaagaagg cacgatttat ggtctgatcg gcccgaacgg cgccggcaag accacgttct tcaacgtgat tacggggctg tacacgcccg actcgggcga attcaagctc gacggcacgc cgtacacgcc gagtgcggtg tatcaggtgg cgaaggcggg catcgcgcgc acgttccaga acattcgtct cttcggcggt atgacggcgc tggagaacgt gatggtcgga cgtcacgttc gcacgaagca cggtctggtc ggcgcggtgt tccagacgcc cgccgaacgc ggcgaagagc gcgagatcaa ggagcgagcg ctggaactgc tcgaatacgt cggcgtggcg cagtacgcgg actacacgtc gcgcaacctg tcatacggtc accagcggcg tctggagatc gcacgtgctc tagcgaccga tccgaagctg ctcgcgctcg acgaaccggc tgccggcatg aatgcgacgg agaaggtcga actgacgaag ctgctcgaca agattcgcgc ggacggcaag acgattctgc tgatcgagca cgacgtgaaa ctggtgatgg gcctgtgtga cgagatgaca gtgctcgact atggcaaggt gattgccgag ggattgccgc angacgtcca gaaggacccg aaggtgatcg aggcttatct gggtgcgggg ggccattaag tgagtaanat aggatccggn tnnnnnnncc ccnnnnnnan nnnnnnnann ntnnctcnnc nngntnnnnn nnnnnnnnnn ntanntnnnn na

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acaacaaaat ccgtctgtcc gtaaaaggcg tgaacaagcg ctttggtggc ttgcaggcgc tgttggacgt gggccttcag atcgaagaag gcacgattta tggtctgatc ggcccgaacg gcgccggcaa gaccacgttc ttcaacgtga ttacggggct gtacacgccc gactcgggcg aattcaagct cgacggcacg ccgtacacgc cgagtgcggt gtatcaggtg gcgaaggcgg gcatcgcgcg cacgttccag aacattcgtc tcttcggcgg tatgacggcg ctggagaacg tgatggtcgg acgtcacgtt cgcacgaagc acggtctggt cggcgcggtg ttccagacgc ccgccgaacg cggcgaagag cgcgagatca aggagcgagc gctggaactg ctcgaatacg tcggcgtggc gcagtacgcg gactacacgt cgcgcaacct gtcatacggt caccagcggc gtctggagat cgcacgtgct ctagcgaccg atccgaagct gctcgcgctc gacgaaccgg ctgccggcat gaatgcgacg gagaaggtcg aactgacgaa gctgctcgac aagattcgcg cggacggcaa gacgattctg ctgatcgagc acgacgtgaa actggtgatg ggcctgtgtg acgagatgac agtgctcgac tatggcaagg tgattgccga gggattgccg caggacgtcc agaaggaccc gaaggtgatc gaggcttatc tgggtgcggg gggccattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at