SSGCID: Target Page

BuviA.00057.h

ABC transporter related

CENTER ID:	BuviA.00057.h
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00057.h.B1.GE35975	Full length( BuviA.00057.h )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6828
RefSeq:	YP_001120403.1
UniProt:	A4JH15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSDKQIRLSV QGVNKRFGGL QALSDVGLQI REGEIYGLIG PNGAGKTTFF NVITGLYTPD SGDFKLDGQN YTPTAVHQVA KAGIARTFQN IRLFGGMTAL ENVMVGRHVR TKHGLLGAVF QTPAERQEER EIKERAIELL DYVGVLQYAD YTSRNLSYGH QRRLEIARAL ATDPKLLALD EPAAGMNATE KVELTRLLDK IRSDGRTILL IEHDVKLVMG LCNRMTVLDY GKVIAEGLPQ DVQKDPKVIE AYLGAGVH

NT Sequence

ATGAGCGACA AGCAAATCCG ATTGTCCGTC CAGGGCGTGA ACAAGCGCTT CGGCGGCCTG CAGGCGCTGT CCGACGTCGG CCTCCAGATC AGGGAAGGCG AGATCTACGG GCTGATCGGC CCGAACGGCG CCGGCAAGAC CACGTTCTTC AACGTGATCA CCGGCCTCTA CACGCCGGAC TCCGGCGACT TCAAGCTGGA CGGCCAGAAC TACACGCCGA CGGCCGTGCA CCAGGTCGCG AAGGCCGGCA TCGCCCGTAC GTTCCAGAAC ATTCGCCTGT TCGGCGGGAT GACGGCGCTC GAGAACGTGA TGGTGGGCCG CCACGTGCGC ACCAAGCACG GCCTGCTCGG CGCGGTGTTC CAGACGCCGG CCGAGCGCCA GGAAGAGCGC GAGATCAAGG AGCGCGCGAT CGAGCTGCTC GATTACGTGG GCGTGCTGCA GTACGCGGAC TACACGTCGC GCAACCTGTC GTACGGCCAC CAGCGCCGTC TCGAGATCGC CCGTGCGCTC GCGACCGATC CGAAGCTGCT CGCGCTCGAC GAGCCGGCGG CCGGGATGAA CGCGACCGAG AAGGTCGAAC TCACGCGCCT GCTCGACAAG ATCCGCTCGG ATGGCCGCAC GATCCTGCTG ATCGAGCACG ACGTGAAGCT CGTGATGGGT CTGTGCAACC GGATGACGGT GCTCGATTAC GGCAAGGTGA TCGCCGAGGG TCTGCCGCAG GACGTGCAGA AGGATCCGAA GGTGATTGAG GCATATCTCG GCGCAGGGGT GCAC

Details for BuviA.00057.h.B1.GE35975

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS DKQIRLSVQG VNKRFGGLQA LSDVGLQIRE GEIYGLIGPN GAGKTTFFNV ITGLYTPDSG DFKLDGQNYT PTAVHQVAKA GIARTFQNIR LFGGMTALEN VMVGRHVRTK HGLLGAVFQT PAERQEEREI KERAIELLDY VGVLQYADYT SRNLSYGHQR RLEIARALAT DPKLLALDEP AAGMNATEKV ELTRLLDKIR SDGRTILLIE HDVKLVMGLC NRMTVLDYGK VIAEGLPXDV QKDPXXIEAY LGAGVH

Validated NT Sequence

attttgtttn ntttaagaag gagatatacc atggctcacc accaccacca ccatatgagc gacaagcaaa tccgattgtc cgtccagggc gtgaacaagc gcttcggcgg cctgcaggcg ctgtccgacg tcggcctcca gatcagggaa ggcgagatct acgggctgat cggcccgaac ggcgccggca agaccacgtt cttcaacgtg atcaccggcc tctacacgcc ggactccggc gacttcaagc tggacggcca gaactacacg ccgacggccg tgcaccaggt cgcgaaggcc ggcatcgccc gtacgttcca gaacattcgc ctgttcggcg ggatgacggc gctcgagaac gtgatggtgg gccgccacgt gcgcaccaag cacggcctgc tcggcgcggt gttccagacg ccggccgagc gccaggaaga gcgcgagatc aaggagcgcg cgatcgagct gctcgattac gtgggcgtgc tgcagtacgc ggactacacg tcgcgcaacc tgtcgtacgg ccaccagcgc cgtctcgaga tcgcccgtgc gctcgcgacc gatccgaagc tgctcgcgct cgacgagccg gcggccggga tgaacgcgac cgagaaggtc gaactcacgc gcctgctcga caagatccgc tcggatggcc gcacgatcct gctgatcgag cacgacgtga agctcgtgat gggtctgtgc aaccggatga cggtgctcga ttacggcaag gtgatcgccg agggtctgcc gcangacgtg cagaaggatc cgaannngat tgaggcatat ctcggcgcng gggtgcacta agtgagtaag ataggatccg gctg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acaagcaaat ccgattgtcc gtccagggcg tgaacaagcg cttcggcggc ctgcaggcgc tgtccgacgt cggcctccag atcagggaag gcgagatcta cgggctgatc ggcccgaacg gcgccggcaa gaccacgttc ttcaacgtga tcaccggcct ctacacgccg gactccggcg acttcaagct ggacggccag aactacacgc cgacggccgt gcaccaggtc gcgaaggccg gcatcgcccg tacgttccag aacattcgcc tgttcggcgg gatgacggcg ctcgagaacg tgatggtggg ccgccacgtg cgcaccaagc acggcctgct cggcgcggtg ttccagacgc cggccgagcg ccaggaagag cgcgagatca aggagcgcgc gatcgagctg ctcgattacg tgggcgtgct gcagtacgcg gactacacgt cgcgcaacct gtcgtacggc caccagcgcc gtctcgagat cgcccgtgcg ctcgcgaccg atccgaagct gctcgcgctc gacgagccgg cggccgggat gaacgcgacc gagaaggtcg aactcacgcg cctgctcgac aagatccgct cggatggccg cacgatcctg ctgatcgagc acgacgtgaa gctcgtgatg ggtctgtgca accggatgac ggtgctcgat tacggcaagg tgatcgccga gggtctgccg caggacgtgc agaaggatcc gaaggtgatt gaggcatatc tcggcgcagg ggtgcactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at