SSGCID: Target Page

BuviA.00057.e

ABC transporter related

CENTER ID:	BuviA.00057.e
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00057.e.B1.GE35335	Full length( BuviA.00057.e )		1	285

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.5058
RefSeq:	YP_001118685.1
UniProt:	A4JC47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNGTALYQFT PPAEDELLAV SGTASMGRVM PAGIDTSHGT ILYLEDIEVS FDGFRALNKL SLAIDAGELR CVIGPNGAGK TTMMDVITGK TRPDAGKVFL GQTIDLARMS EPEIARAGIG RKFQKPTVFE QHPVWENLEL AMQTDKGWLA SLRARLDRAA QAKIEDTLTL IGLESDAYRA AGELSHGQKQ RLEIGMLLMQ RPALLLLDEP AAGMTDHETM ELAKLLNTLR GTCSMMVVEH DMEFVAALAG DTGRVTVMAE GAVLAEGTLD QVKRDDAVIE SYLGR

NT Sequence

ATGAACGGCA CAGCGCTCTA CCAGTTCACG CCGCCAGCCG AAGACGAGCT GCTCGCGGTC AGCGGCACCG CGTCGATGGG CCGCGTGATG CCCGCCGGCA TCGACACGTC GCACGGCACG ATCCTGTATC TCGAGGACAT CGAGGTGAGC TTCGACGGCT TTCGCGCGCT GAACAAGCTG TCGCTCGCGA TCGACGCGGG CGAGCTGCGC TGCGTGATCG GCCCGAACGG CGCGGGCAAG ACGACGATGA TGGACGTCAT CACCGGCAAG ACGCGCCCCG ATGCGGGCAA GGTGTTTCTC GGCCAGACCA TCGACCTCGC GCGGATGAGC GAACCCGAGA TCGCGCGCGC CGGGATCGGC CGCAAGTTTC AGAAGCCGAC CGTGTTCGAG CAGCATCCGG TGTGGGAAAA CCTCGAACTC GCGATGCAGA CCGACAAGGG CTGGCTCGCG TCGCTGCGCG CGCGGCTCGA CCGCGCGGCG CAGGCGAAGA TCGAGGACAC GCTCACGCTG ATCGGCCTCG AAAGCGATGC GTATCGCGCG GCCGGCGAAC TGTCGCACGG CCAGAAGCAG CGGCTCGAGA TCGGCATGCT GCTGATGCAG CGCCCCGCGC TGCTGCTGCT CGACGAGCCC GCGGCCGGGA TGACCGACCA CGAGACGATG GAGCTCGCGA AGCTGCTGAA CACGCTGCGC GGCACCTGCT CGATGATGGT CGTCGAGCAC GACATGGAGT TCGTCGCCGC GCTCGCGGGC GACACGGGCC GCGTGACGGT GATGGCCGAA GGCGCGGTGC TCGCGGAAGG CACGCTCGAC CAGGTCAAGC GCGACGACGC GGTGATCGAG TCATACCTCG GACGA

Details for BuviA.00057.e.B1.GE35335

HARVESTED ON:	5/16/2012
SEQUENCED ON:	5/15/2012
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN GTALYQFTPP AEDELLAVSG TASMGRVMPA GIDTSHGTIL YLEDIEVSFD GFRALNKLSL AIDAGELRCV IGPNGAGKTT MMDVITGKTR PDAGKVFLGQ TIDLARMSEP EIARAGIGRK FQKPTVFEQH PVWENLELAM QTDKGWLASL RARLDRAAQA KIEDTLTLIG LESDAYRAAG ELSHGQKQRL EIGMLLMQRP ALLLLDEPAA GMTDHETMEL AKLLNTLRGT CSMMVVEHDM EFVAALAGDT GRVTVMAEGA VLAEGTLDQV KRDDAVIESY LGR

Validated NT Sequence

ttttgtnnnn nttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg gcacagcgct ctaccagttc acgccgccag ccgaagacga gctgctcgcg gtcagcggca ccgcgtcgat gggccgcgtg atgcccgccg gcatcgacac gtcgcacggc acgatcctgt atctcgagga catcgaggtg agcttcgacg gctttcgcgc gctgaacaag ctgtcgctcg cgatcgacgc gggcgagctg cgctgcgtga tcggcccgaa cggcgcgggc aagacgacga tgatggacgt catcaccggc aagacgcgcc ccgatgcggg caaggtgttt ctcggccaga ccatcgacct cgcgcggatg agcgaacccg agatcgcgcg cgccgggatc ggccgcaagt ttcagaagcc gaccgtgttc gagcagcatc cggtgtggga aaacctcgaa ctcgcgatgc agaccgacaa gggctggctc gcgtcgctgc gcgcgcggct cgaccgcgcg gcgcaggcga agatcgagga cacgctcacg ctgatcggcc tcgaaagcga tgcgtatcgc gcggccggcg aactgtcgca cggccagaag cagcggctcg agatcggcat gctgctgatg cagcgccccg cgctgctgct gctcgacgag cccgcggccg ggatgaccga ccacgagacg atggagctcg cgaagctgct gaacacgctg cgcggcacct gctcgatgat ggtcgtcgag cacgacatgg agttcgtcgc cgcgctcgcg ggcgacacgg gccgcgtgac ggtgatggcc gaaggcgcgg tgctcgcgga aggnacgctc gaccaggtca agcgcgacga cgcggtgatc gagtcntacc tcggacgata agtgagtaan anaggatccg nnnnnnnnnn nncccnnnnn nnnnnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg gcacagcgct ctaccagttc acgccgccag ccgaagacga gctgctcgcg gtcagcggca ccgcgtcgat gggccgcgtg atgcccgccg gcatcgacac gtcgcacggc acgatcctgt atctcgagga catcgaggtg agcttcgacg gctttcgcgc gctgaacaag ctgtcgctcg cgatcgacgc gggcgagctg cgctgcgtga tcggcccgaa cggcgcgggc aagacgacga tgatggacgt catcaccggc aagacgcgcc ccgatgcggg caaggtgttt ctcggccaga ccatcgacct cgcgcggatg agcgaacccg agatcgcgcg cgccgggatc ggccgcaagt ttcagaagcc gaccgtgttc gagcagcatc cggtgtggga aaacctcgaa ctcgcgatgc agaccgacaa gggctggctc gcgtcgctgc gcgcgcggct cgaccgcgcg gcgcaggcga agatcgagga cacgctcacg ctgatcggcc tcgaaagcga tgcgtatcgc gcggccggcg aactgtcgca cggccagaag cagcggctcg agatcggcat gctgctgatg cagcgccccg cgctgctgct gctcgacgag cccgcggccg ggatgaccga ccacgagacg atggagctcg cgaagctgct gaacacgctg cgcggcacct gctcgatgat ggtcgtcgag cacgacatgg agttcgtcgc cgcgctcgcg ggcgacacgg gccgcgtgac ggtgatggcc gaaggcgcgg tgctcgcgga aggcacgctc gaccaggtca agcgcgacga cgcggtgatc gagtcatacc tcggacgatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat