BuviA.00048.a
Putative GAF sensor protein
| CENTER ID: | BuviA.00048.a |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00048.a.B1.GE38086 | Full length( BuviA.00048.a ) | 1 | 170 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5HL6
DEPOSITED: 1/14/2016
DETERMINATION: XRay
CLONE: BuviA.00048.a.B1.GE38086
PROTEIN: BuviA.00048.a.B1.PS02344
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|269482.11.peg.5302 |
| RefSeq: | YP_001118914.1 |
| UniProt: | A4JCS6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTLSTDPHA SKAELYATLA EQARSLVESE PDLIANAANF SALVYHSLDR LNWAGFYFFD GTELVVGPFQ GKPACVRIAL GKGVCGTAAQ TRQTQVVRDV HAFPGHIACD AASESEIVVP LVAADGTLIG VWDVDSPVAA RFDDEDRSGM EALCRVFVEH AWQKARDRAA
NT Sequence
ATGTTCACGC TGTCCACCGA CCCTCACGCC TCCAAGGCCG AACTGTATGC GACGCTCGCC GAGCAGGCCC GCTCGCTCGT CGAATCGGAG CCGGACCTGA TCGCAAACGC CGCGAATTTC TCCGCGCTCG TCTATCACTC GCTCGATCGT CTGAACTGGG CCGGCTTCTA TTTCTTCGAC GGGACGGAGC TGGTGGTCGG CCCGTTCCAG GGCAAGCCGG CCTGCGTGCG CATCGCGCTC GGCAAGGGCG TGTGCGGCAC GGCGGCGCAG ACGCGCCAAA CGCAGGTCGT GCGCGACGTG CATGCGTTCC CCGGCCATAT CGCGTGCGAT GCCGCGTCGG AGTCGGAAAT CGTCGTGCCG CTGGTAGCGG CAGACGGCAC GCTGATCGGC GTGTGGGACG TCGACAGCCC GGTCGCCGCA CGTTTCGACG ACGAAGACCG CAGCGGGATG GAAGCGCTGT GCCGCGTGTT CGTCGAACAC GCGTGGCAAA AGGCGCGCGA CCGAGCAGCG
Details for BuviA.00048.a.B1.GE38086
| HARVESTED ON: | 5/13/2014 |
| SEQUENCED ON: | 5/6/2014 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 19kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMF TLSTDPHASK AELYATLAEQ ARSLVESEPD LIANAANFSA LVYHSLDRLN WAGFYFFDGT ELVVGPFQGK PACVRIALGK GVCGTAAQTR QTQVVRDVHA FPGHIACDAA SESEIVVPLV AADGTLIGVW DVDSPVAARF DDEDRSGMEA LCRVFVEHAW QKARDRAA
Validated NT Sequence
ctnttnnnan nnnggagana taccatggct caccaccacc accaccatat gttcacgctg tccaccgacc ctcacgcctc caaggccgaa ctgtatgcga cgctcgccga gcaggcccgc tcgctcgtcg aatcggagcc ggacctgatc gcaaacgccg cgaatttctc cgcgctcgtc tatcactcgc tcgatcgtct gaactgggcc ggcttctatt tcttcgacgg gacggagctg gtggtcggcc cgttccaggg caagccggcc tgcgtgcgca tcgcgctcgg caagggcgtg tgcggcacgg cggcgcagac gcgccaaacg caggtcgtgc gcgacgtgca tgcgttcccc ggccatatcg cgtgcgatgc cgcgtcggag tcggaaatcg tcgtgccgct ggtagcggca gacggcacgc tgatcggcgt gtgggacgtc gacagcccgg tcgccgcacg tttcgacgac gaagaccgca gcgggatgga agcgctgtgc cgcgtgttcg tcgaacacgc gtggcaaaag gcgcgcgacc gagcagcgta agtgagtaag ataggatccg gctgctaaca agcccccgaa aga
Expected Protein Sequence
MAHHHHHHMF TLSTDPHASK AELYATLAEQ ARSLVESEPD LIANAANFSA LVYHSLDRLN WAGFYFFDGT ELVVGPFQGK PACVRIALGK GVCGTAAQTR QTQVVRDVHA FPGHIACDAA SESEIVVPLV AADGTLIGVW DVDSPVAARF DDEDRSGMEA LCRVFVEHAW QKARDRAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca cgctgtccac cgaccctcac gcctccaagg ccgaactgta tgcgacgctc gccgagcagg cccgctcgct cgtcgaatcg gagccggacc tgatcgcaaa cgccgcgaat ttctccgcgc tcgtctatca ctcgctcgat cgtctgaact gggccggctt ctatttcttc gacgggacgg agctggtggt cggcccgttc cagggcaagc cggcctgcgt gcgcatcgcg ctcggcaagg gcgtgtgcgg cacggcggcg cagacgcgcc aaacgcaggt cgtgcgcgac gtgcatgcgt tccccggcca tatcgcgtgc gatgccgcgt cggagtcgga aatcgtcgtg ccgctggtag cggcagacgg cacgctgatc ggcgtgtggg acgtcgacag cccggtcgcc gcacgtttcg acgacgaaga ccgcagcggg atggaagcgc tgtgccgcgt gttcgtcgaa cacgcgtggc aaaaggcgcg cgaccgagca gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat