BuviA.00046.c

Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

CENTER ID: BuviA.00046.c
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00046.c.B1.GE38084 Full length( BuviA.00046.c ) 1 468
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3726
RefSeq: YP_001117415.1
UniProt: A4JNU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLVVVSNR IADPRKAAAG GLAVAVKDSL QESGGVWFGW SGRLRSEGER PAQGDDVQIQ NVGGIQLATI DLDPQDYDAY YLGYSNNVLW PVFHYRLDLA QFDRRFADGY RRVNQLFARK LSTLLRPDDT IWVHDYQLIP LAAELRAMGC TNPIGFFLHI PMPPPPIMAA IPEHEWLMRA LFAYDLIGFQ TESDLLHFEH YVEAEAGAAR LPDGRLRAFG RTLSAGAFPI GINVDEFAAL AENRDGIDMF ERMRDEYSRR QLLVGVDRLD YTKGLPQRVH AFRQLLEQYP ENRDRATLIQ IAAPSREDLG AYDDLRREMD SLCGAINGDY GELEWMPMRY IHRTVARKRL PGLYRASRVA LVTPLRDGMN LVAKEFLAAQ DEADPGVLVL SRFAGAAEQL KPALLVNPYD TQGTAQAIQR ALTMPLDERR QRHNALMAIV RKTDVHWWRT RFLDALTEAA EVAAAAAS
NT Sequence
GTGAGCCGAC TCGTCGTCGT ATCCAATCGC ATTGCAGATC CCCGTAAAGC CGCCGCCGGC GGCCTTGCCG TAGCCGTGAA AGACAGCCTG CAGGAATCGG GCGGCGTCTG GTTCGGCTGG AGCGGCAGGC TGCGCAGCGA GGGCGAGCGA CCCGCGCAGG GCGACGACGT GCAGATCCAG AACGTCGGCG GGATCCAGCT CGCGACGATC GATCTCGATC CGCAGGATTA CGACGCGTAC TATCTCGGCT ACTCGAACAA CGTGCTATGG CCGGTGTTCC ACTACCGGCT CGATCTCGCG CAGTTCGACC GCCGCTTCGC CGACGGCTAC CGACGCGTGA ACCAGCTGTT CGCGCGCAAG CTCAGCACGC TGCTGCGGCC GGACGACACC ATCTGGGTTC ACGACTATCA ACTGATTCCG CTCGCCGCCG AACTGCGCGC GATGGGCTGC ACGAATCCGA TCGGCTTCTT CCTGCATATT CCGATGCCGC CGCCGCCGAT CATGGCCGCG ATTCCGGAGC ACGAATGGCT GATGCGCGCG CTGTTTGCGT ACGACCTCAT CGGCTTTCAG ACCGAATCCG ATCTGCTGCA CTTCGAGCAC TACGTCGAGG CCGAGGCCGG CGCCGCGCGT CTGCCCGACG GTCGCCTGCG TGCGTTCGGC CGCACGCTGT CGGCCGGCGC GTTTCCGATC GGCATCAACG TCGACGAATT CGCGGCGCTG GCGGAGAATC GCGACGGCAT CGACATGTTC GAGCGGATGC GCGATGAATA TTCGCGCCGT CAATTGCTCG TCGGCGTCGA TCGTCTCGAT TACACGAAGG GCCTGCCGCA ACGCGTGCAT GCGTTCCGTC AGTTGCTCGA ACAGTATCCG GAGAATCGCG ACCGCGCGAC GCTGATCCAG ATCGCCGCGC CGAGCCGCGA GGATCTCGGC GCCTACGACG ACCTGCGGCG CGAGATGGAC AGTCTGTGCG GTGCGATCAA CGGCGACTAC GGCGAGCTCG AGTGGATGCC AATGCGCTAC ATTCATCGGA CCGTTGCGCG CAAGCGCTTG CCCGGCCTCT ATCGCGCAAG CCGTGTCGCG CTCGTTACGC CGCTGCGAGA CGGGATGAAC CTGGTCGCGA AGGAGTTTCT TGCCGCGCAG GACGAGGCTG ATCCCGGCGT GCTCGTGCTG TCGCGCTTCG CCGGCGCAGC CGAGCAACTG AAGCCCGCGC TGCTCGTCAA TCCGTACGAC ACGCAGGGCA CCGCGCAGGC GATTCAACGC GCACTCACGA TGCCGCTCGA CGAGCGCCGC CAGCGCCACA ACGCGCTGAT GGCGATCGTG CGCAAGACCG ACGTCCACTG GTGGCGCACG CGCTTTCTCG ATGCGCTGAC CGAAGCGGCC GAAGTTGCGG CGGCGGCTGC GTCC
Details for BuviA.00046.c.B1.GE38084
HARVESTED ON: 5/13/2014
SEQUENCED ON: 5/6/2014
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RLVVVSNRIA DPRKAAAGGL AVAVKDSLQE SGGVWFGWSG RLRSEGERPA QGDDVQIQNV GGIQLATIDL DPQDYDAYYL GYSNNVLWPV FHYRLDLAQF DRRFADGYRR VNQLFARKLS TLLRPDDTIW VHDYQLIPLA AELRAMGCTN PIGFFLHIPM PPPPIMAAIP EHEWLMRALF AYDLIGFQTE SDLLHFEHYV EAEAGAARLP DGRLRAFGRT LSAGAFPIGI NVDEFAALAE NRDGIDMFER MRDEYSRRQL LVGVDRLDYT KGLPQRVHAF RQLLEQYPEN RDRATLIQIA APSREDLGAY DDLRREMDSL CGAINGDYGE LEWMPMRYIH RTVARKRLPG LYRASRVALV TPLRDGMNLV AKEFLAAQDE ADPGVLVLSR FAGAAEQLKP ALLVNPYDTQ GTAQAIQRAL TMPLDERRQR HNALMAIVRK TDVHWWRTRF LDALTEAAEV AAAAAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagcc gactcgtcgt cgtatccaat cgcattgcag atccccgtaa agccgccgcc ggcggccttg ccgtagccgt gaaagacagc ctgcaggaat cgggcggcgt ctggttcggc tggagcggca ggctgcgcag cgagggcgag cgacccgcgc agggcgacga cgtgcagatc cagaacgtcg gcgggatcca gctcgcgacg atcgatctcg atccgcagga ttacgacgcg tactatctcg gctactcgaa caacgtgcta tggccggtgt tccactaccg gctcgatctc gcgcagttcg accgccgctt cgccgacggc taccgacgcg tgaaccagct gttcgcgcgc aagctcagca cgctgctgcg gccggacgac accatctggg ttcacgacta tcaactgatt ccgctcgccg ccgaactgcg cgcgatgggc tgcacgaatc cgatcggctt cttcctgcat attccgatgc cgccgccgcc gatcatggcc gcgattccgg agcacgaatg gctgatgcgc gcgctgtttg cgtacgacct catcggcttt cagaccgaat ccgatctgct gcacttcgag cactacgtcg aggccgaggc cggcgccgcg cgtctgcccg acggtcgcct gcgtgcgttc ggccgcacgc tgtcggccgg cgcgtttccg atcggcatca acgtcgacga attcgcggcg ctggcggaga atcgcgacgg catcgacatg ttcgagcgga tgcgcgatga atattcgcgc cgtcaattgc tcgtcggcgt cgatcgtctc gattacacga agggcctgcc gcaacgcgtg catgcgttcc gtcagttgct cgaacagtat ccggagaatc gcgaccgcgc gacgctgatc cagatcgccg cgccgagccg cgaggatctc ggcgcctacg acgacctgcg gcgcgagatg gacagtctgt gcggtgcgat caacggcgac tacggcgagc tcgagtggat gccaatgcgc tacattcatc ggaccgttgc gcgcaagcgc ttgcccggcc tctatcgcgc aagccgtgtc gcgctcgtta cgccgctgcg agacgggatg aacctggtcg cgaaggagtt tcttgccgcg caggacgagg ctgatcccgg cgtgctcgtg ctgtcgcgct tcgccggcgc agccgagcaa ctgaagcccg cgctgctcgt caatccgtac gacacgcagg gcaccgcgca ggcgattcaa cgcgcactca cgatgccgct cgacgagcgc cgccagcgcc acaacgcgct gatggcgatc gtgcgcaaga ccgacgtcca ctggtggcgc acgcgctttc tcgatgcgct gaccgaagcg gccgaagttg cggcggcggc tgcgtcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at