SSGCID: Target Page

BuviA.00027.b

Acyl-CoA dehydrogenase domain protein

CENTER ID:	BuviA.00027.b
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00027.b.B1.GE39409	Full length( BuviA.00027.b )		1	385

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.7239
RefSeq:	YP_001120800.1
UniProt:	A4JI62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFQHTEDRR MLADTLNRFI AEQYAFPVRD RIARSPQGCD RAMWRRFAEL GAVGALFPEA DGGFGGAGFD IAVVFECLGR GLVVEPFLGA LLAGRALLLG GGGGAQRERL AQLIEGRACA AFAHDEPSSH YELTTVRTRA ERSGDGWVLT GAKGVVDQAA QASFFVVSAR TAGRDDDEHG IGLFVVPADA PGVSWRDYRK IDGGRAAEVR FERVALRGDA ALVDTHDAAA GDDGQGAALL ERVLGYGLLA LSAEALGAMD VAKEATLDYL RTRRQFGMPI GSFQALQHRM ADLLLEVEQA RSAVINAAAQ LDAPRTVRER ALAAAKYSIG RIGTLVAEES IQLHGGIGMT WELPLSHYAK RLVMIDHQLG DEDHHLARYI ALSKR

NT Sequence

ATGGATTTTC AGCACACGGA AGACCGGCGG ATGCTGGCGG ACACGTTGAA CCGCTTCATC GCCGAACAGT ATGCGTTCCC GGTGCGCGAC CGCATCGCGC GGTCGCCGCA AGGGTGCGAC CGCGCGATGT GGCGGCGCTT CGCCGAACTG GGCGCGGTCG GCGCGCTGTT TCCGGAGGCG GACGGCGGCT TCGGCGGCGC GGGTTTCGAT ATCGCGGTGG TGTTCGAATG CCTCGGCCGC GGGCTCGTCG TCGAGCCGTT TCTCGGCGCG CTGCTCGCGG GCCGCGCGCT GCTGCTGGGC GGTGGCGGCG GCGCGCAGCG CGAACGGCTC GCGCAGTTGA TCGAGGGCCG CGCGTGCGCC GCGTTCGCGC ACGACGAACC GAGCTCGCAC TACGAACTGA CGACCGTGCG CACGCGCGCC GAGCGCAGCG GCGACGGCTG GGTGCTGACC GGTGCGAAGG GCGTCGTCGA CCAGGCCGCG CAGGCGTCGT TCTTCGTCGT CAGCGCGCGC ACGGCCGGCC GCGACGACGA CGAGCACGGC ATCGGCCTGT TCGTCGTGCC GGCCGATGCG CCTGGCGTGT CGTGGCGCGA CTACCGGAAG ATCGACGGCG GCCGCGCGGC CGAGGTGCGC TTCGAACGCG TCGCGCTGCG CGGCGATGCG GCGCTGGTCG ACACGCACGA CGCGGCCGCC GGCGACGACG GGCAGGGCGC CGCGCTGCTC GAACGCGTGC TCGGCTACGG GCTGCTCGCG CTGTCGGCTG AGGCGCTCGG CGCGATGGAC GTCGCGAAGG AGGCGACGCT CGACTATCTG CGCACGCGCC GGCAGTTCGG GATGCCGATC GGCAGCTTCC AGGCGCTGCA GCACCGGATG GCCGACCTGC TGCTCGAAGT CGAGCAGGCG CGCTCGGCGG TGATCAACGC GGCCGCGCAG CTCGACGCGC CGCGCACGGT GCGCGAACGC GCGCTCGCGG CGGCCAAGTA CAGCATCGGC CGGATCGGCA CGCTGGTCGC GGAGGAGAGC ATCCAGCTGC ACGGCGGGAT CGGGATGACG TGGGAGCTGC CGCTGTCGCA TTACGCGAAG CGGCTGGTGA TGATCGATCA CCAGCTCGGC GACGAGGACC ATCATCTGGC GCGCTACATC GCGCTGTCGA AACGA

Details for BuviA.00027.b.B1.GE39409

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD FQHTEDRRML ADTLNRFIAE QYAFPVRDRI ARSPQGCDRA MWRRFAELGA VGALFPEADG GFGGAGFDIA VVFECLGRGL VVEPFLGALL AGRALLLGGG GGAQRERLAQ LIEGRACAAF AHDEPSSHYE LTTVRTRAER SGDGWVLTGA KGVVDQAAQA SFFVVSARTA GRDDDEHGIG LFVVPADAPG VSWRDYRKID GGRAAEVRFE RVALRGDAAL VDTHDAAAGD DGQGAALLER VLGYGLLALS AEALGAMDVA KEATLDYLRT RRQFGMPIGS FQALQHRMAD LLLEVEQARS AVINAAAQLD APRTVRERAL AAAKYSIGRI GTLVAEESIQ LHGGIGMTWE LPLSHYAKRL VMIDHQLGDE DHH

Validated NT Sequence

agatgatggt cctcgtcgcc gagctggtga tcgatcatca ccagccgctt cgcgtaatgc gacagcggca gctcccacgt catcccgatc ccgccgtgca gctggatgct ctcctccgcg accagcgtgc cgatccggcc gatgctgtac ttggccgccg cgagcgcgcg ttcgcgcacc gtgcgcggcg cgtcgagctg cgcggccgcg ttgatcaccg ccgagcgcgc ctgctcgact tcgagcagca ggtcggccat ccggtgctgc agcgcctgga agctgccgat cggcatcccg aactgccggc gcgtgcgcag atagtcgagc gtcgcctcct tcgcgacgtc catcgcgccg agcgcctcag ccgacagcgc gagcagcccg tagccgagca cgcgttcgag cagcgcggcg ccctgcccgt cgtcgccggc ggccgcgtcg tgcgtgtcga ccagcgccgc atcgccgcgc agcgcgacgc gttcgaagcg cacctcggcc gcgcggccgc cgtcgatctt ccggtagtcg cgccacgaca cgccaggcgc atcggccggc acgacgaaca ggccgatgcc gtgctcgtcg tcgtcgcggc cggccgtgcg cgcgctgacg acgaagaacg acgcctgcgc ggcctggtcg acgacgccct tcgcaccggt cagcacccag ccgtcgccgc tgcgctcggc gcgcgtgcgc acggtcgtca gttcgtagtg cgagctcggt tcgtcgtgcg cgaacgcggc gcacgcgcgg ccctcgatca actgcgcgag ccgttcgcgc tgcgcgccgc cgccaccgcc cagcagcagc gcgcggcccg cgagcagcgc gccgagaaac ggctcgacga cgagcccgcg gccgaggcat tcgaacacca ccgcgatatc gaaacccgcg ccgccgaagc cgccgtccgc ctccggaaac agcgcgccga ccgcgcccag ttcggcgaag cgccgccaca tcgcgcggtc gcacccttgc ggcgaccgcg cgatgcggtc gcgcaccggg aacgcatact gttcggcgat gaagcggttc aacgtgtccg ccagcatccg ccggtcttcc gtgtgctgaa aatccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt ttcagcacac ggaagaccgg cggatgctgg cggacacgtt gaaccgcttc atcgccgaac agtatgcgtt cccggtgcgc gaccgcatcg cgcggtcgcc gcaagggtgc gaccgcgcga tgtggcggcg cttcgccgaa ctgggcgcgg tcggcgcgct gtttccggag gcggacggcg gcttcggcgg cgcgggtttc gatatcgcgg tggtgttcga atgcctcggc cgcgggctcg tcgtcgagcc gtttctcggc gcgctgctcg cgggccgcgc gctgctgctg ggcggtggcg gcggcgcgca gcgcgaacgg ctcgcgcagt tgatcgaggg ccgcgcgtgc gccgcgttcg cgcacgacga accgagctcg cactacgaac tgacgaccgt gcgcacgcgc gccgagcgca gcggcgacgg ctgggtgctg accggtgcga agggcgtcgt cgaccaggcc gcgcaggcgt cgttcttcgt cgtcagcgcg cgcacggccg gccgcgacga cgacgagcac ggcatcggcc tgttcgtcgt gccggccgat gcgcctggcg tgtcgtggcg cgactaccgg aagatcgacg gcggccgcgc ggccgaggtg cgcttcgaac gcgtcgcgct gcgcggcgat gcggcgctgg tcgacacgca cgacgcggcc gccggcgacg acgggcaggg cgccgcgctg ctcgaacgcg tgctcggcta cgggctgctc gcgctgtcgg ctgaggcgct cggcgcgatg gacgtcgcga aggaggcgac gctcgactat ctgcgcacgc gccggcagtt cgggatgccg atcggcagct tccaggcgct gcagcaccgg atggccgacc tgctgctcga agtcgagcag gcgcgctcgg cggtgatcaa cgcggccgcg cagctcgacg cgccgcgcac ggtgcgcgaa cgcgcgctcg cggcggccaa gtacagcatc ggccggatcg gcacgctggt cgcggaggag agcatccagc tgcacggcgg gatcgggatg acgtgggagc tgccgctgtc gcattacgcg aagcggctgg tgatgatcga tcaccagctc ggcgacgagg accatcatct ggcgcgctac atcgcgctgt cgaaacgatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat