SSGCID: Target Page

BuviA.00027.a

Acyl-CoA dehydrogenase domain protein

CENTER ID:	BuviA.00027.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00027.a.B1.GE39408	Full length( BuviA.00027.a )		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.6557
RefSeq:	YP_001120151.1
UniProt:	A4JGB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLNYSPADD AFRADVRAWL EANLPHALRA KVLDHKRLNR DDFASWHRIL GERGWSAPAW PVEYGGPGWN ATQRHIWEDE CARIGAPIVL PFGVSMVAPV LMKYGSEAQK RHYLPRILDG TDWWCQGYSE PGSGSDLASL RTRAERDGDH YVVNGQKTWT TLGQYADMMF CLVRTDSSAK KQEGISFLLI DMKTPGITVR PIITLDEDHE VNEVFFEDVK VPVENLVGDE NRGWTYAKYL LGHERTGIAR VGASKRELAF LKRVASNQRK NGKPLLADPV FAAKVAALEV ELMALEVTVL RVVSRETSGK GPGPEASMLK IKGTEVQQAL TELMFEAIGP LAAPFDIPFL DGEREHSVAG DDDAAPLAAY YFNYRKTSIY GGSNEIQKNI IAQMILGL

NT Sequence

ATGGATCTGA ATTACTCCCC CGCCGACGAC GCATTCCGCG CCGACGTTCG CGCCTGGCTC GAGGCGAATC TGCCTCATGC ACTGCGCGCC AAGGTACTCG ACCATAAACG GCTGAACCGC GACGATTTCG CAAGCTGGCA CCGCATTCTC GGCGAGCGCG GCTGGTCCGC GCCCGCGTGG CCGGTCGAGT ACGGCGGTCC GGGCTGGAAC GCGACGCAGC GGCACATCTG GGAAGATGAG TGCGCCCGCA TCGGCGCGCC GATCGTGCTG CCGTTCGGCG TGTCGATGGT CGCGCCGGTG CTGATGAAAT ACGGCAGCGA AGCGCAGAAG CGCCACTACC TGCCGCGCAT CCTCGACGGC ACCGACTGGT GGTGCCAGGG CTATTCGGAG CCGGGTTCCG GTTCGGATCT CGCCTCGCTG CGCACGCGCG CCGAACGCGA CGGCGACCAC TACGTGGTGA ATGGCCAGAA GACCTGGACG ACGCTCGGCC AATATGCGGA CATGATGTTC TGCCTCGTGC GCACCGATTC GTCGGCGAAG AAGCAGGAAG GCATCTCGTT CCTGCTGATC GACATGAAGA CGCCGGGCAT CACGGTGCGC CCGATCATCA CGCTCGACGA GGACCACGAA GTCAACGAGG TGTTCTTCGA GGACGTGAAG GTGCCGGTCG AGAATCTCGT CGGCGACGAG AACCGCGGCT GGACCTATGC CAAATATCTG CTGGGGCACG AGCGCACCGG CATCGCGCGC GTCGGCGCGT CGAAGCGCGA GCTGGCCTTC CTGAAACGCG TCGCATCGAA CCAGCGCAAG AACGGCAAGC CGTTGCTCGC CGATCCCGTG TTCGCCGCGA AGGTCGCGGC GCTGGAGGTC GAGCTGATGG CGCTCGAGGT GACGGTGCTG CGGGTCGTGA GCCGTGAGAC GAGCGGCAAG GGGCCGGGTC CCGAGGCGTC GATGCTGAAG ATCAAGGGCA CCGAGGTCCA GCAGGCGCTC ACCGAGCTGA TGTTCGAAGC GATCGGGCCG CTCGCCGCGC CGTTCGACAT CCCGTTCCTC GACGGCGAGC GCGAGCACAG CGTCGCGGGC GACGACGACG CGGCGCCGCT CGCCGCGTAC TACTTCAATT ACCGGAAGAC GTCGATCTAC GGCGGTTCGA ACGAGATTCA GAAGAACATC ATCGCGCAGA TGATTCTGGG GCTG

Details for BuviA.00027.a.B1.GE39408

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD LNYSPADDAF RADVRAWLEA NLPHALRAKV LDHKRLNRDD FASWHRILGE RGWSAPAWPV EYGGPGWNAT QRHIWEDECA RIGAPIVLPF GVSMVAPVLM KYGSEAQKRH YLPRILDGTD WWCQGYSEPG SGSDLASLRT RAERDGDHYV VNGQKTWTTL GQYADMMFCL VRTDSSAKKQ EGISFLLIDM KTPGITVRPI ITLDEDHEVN EVFFEDVKVP VENLVGDENR GWTYAKYLLG HERTGIARVG ASKRELAFLK RVASNQRKNG KPLLADPVFA AKVAALEVEL MALEVTVLRV VSRETSGKGP GPEASMLKIK GTEVQQALTE LMFEAIGPLA APFDIPFLDG EREHSVAGDD DAAPLAAYYF NYRKTSIYGG SNEIQKN

Validated NT Sequence

gatgttcttc tgaatctcgt tcgaaccgcc gtagatcgac gtcttccggt aattgaagta gtacgcggcg agcggcgccg cgtcgtcgtc gcccgcgacg ctgtgctcgc gctcgccgtc gaggaacggg atgtcgaacg gcgcggcgag cggcccgatc gcttcgaaca tcagctcggt gagcgcctgc tggacctcgg tgcccttgat cttcagcatc gacgcctcgg gacccggccc cttgccgctc gtctcacggc tcacgacccg cagcaccgtc acctcgagcg ccatcagctc gacctccagc gccgcgacct tcgcggcgaa cacgggatcg gcgagcaacg gcttgccgtt cttgcgctgg ttcgatgcga cgcgtttcag gaaggccagc tcgcgcttcg acgcgccgac gcgcgcgatg ccggtgcgct cgtgccccag cagatatttg gcataggtcc agccgcggtt ctcgtcgccg acgagattct cgaccggcac cttcacgtcc tcgaagaaca cctcgttgac ttcgtggtcc tcgtcgagcg tgatgatcgg gcgcaccgtg atgcccggcg tcttcatgtc gatcagcagg aacgagatgc cttcctgctt cttcgccgac gaatcggtgc gcacgaggca gaacatcatg tccgcatatt ggccgagcgt cgtccaggtc ttctggccat tcaccacgta gtggtcgccg tcgcgttcgg cgcgcgtgcg cagcgaggcg agatccgaac cggaacccgg ctccgaatag ccctggcacc accagtcggt gccgtcgagg atgcgcggca ggtagtggcg cttctgcgct tcgctgccgt atttcatcag caccggcgcg accatcgaca cgccgaacgg cagcacgatc ggcgcgccga tgcgggcgca ctcatcttcc cagatgtgcc gctgcgtcgc gttccagccc ggaccgccgt actcgaccgg ccacgcgggc gcggaccagc cgcgctcgcc gagaatgcgg tgccagcttg cgaaatcgtc gcggttcagc cgtttatggt cgagtacctt ggcgcgcagt gcatgaggca gattcgcctc gagccaggcg cgaacgtcgg cgcggaatgc gtcgtcggcg ggggagtaat tcagatccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tgaattactc ccccgccgac gacgcattcc gcgccgacgt tcgcgcctgg ctcgaggcga atctgcctca tgcactgcgc gccaaggtac tcgaccataa acggctgaac cgcgacgatt tcgcaagctg gcaccgcatt ctcggcgagc gcggctggtc cgcgcccgcg tggccggtcg agtacggcgg tccgggctgg aacgcgacgc agcggcacat ctgggaagat gagtgcgccc gcatcggcgc gccgatcgtg ctgccgttcg gcgtgtcgat ggtcgcgccg gtgctgatga aatacggcag cgaagcgcag aagcgccact acctgccgcg catcctcgac ggcaccgact ggtggtgcca gggctattcg gagccgggtt ccggttcgga tctcgcctcg ctgcgcacgc gcgccgaacg cgacggcgac cactacgtgg tgaatggcca gaagacctgg acgacgctcg gccaatatgc ggacatgatg ttctgcctcg tgcgcaccga ttcgtcggcg aagaagcagg aaggcatctc gttcctgctg atcgacatga agacgccggg catcacggtg cgcccgatca tcacgctcga cgaggaccac gaagtcaacg aggtgttctt cgaggacgtg aaggtgccgg tcgagaatct cgtcggcgac gagaaccgcg gctggaccta tgccaaatat ctgctggggc acgagcgcac cggcatcgcg cgcgtcggcg cgtcgaagcg cgagctggcc ttcctgaaac gcgtcgcatc gaaccagcgc aagaacggca agccgttgct cgccgatccc gtgttcgccg cgaaggtcgc ggcgctggag gtcgagctga tggcgctcga ggtgacggtg ctgcgggtcg tgagccgtga gacgagcggc aaggggccgg gtcccgaggc gtcgatgctg aagatcaagg gcaccgaggt ccagcaggcg ctcaccgagc tgatgttcga agcgatcggg ccgctcgccg cgccgttcga catcccgttc ctcgacggcg agcgcgagca cagcgtcgcg ggcgacgacg acgcggcgcc gctcgccgcg tactacttca attaccggaa gacgtcgatc tacggcggtt cgaacgagat tcagaagaac atcatcgcgc agatgattct ggggctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at