BuviA.00024.a

Taurine dioxygenase

CENTER ID: BuviA.00024.a
ORGANISM: Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuviA.00024.a.B1.GE38077 Full length( BuviA.00024.a ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|269482.11.peg.3699
RefSeq: YP_001117391.1
UniProt: A4JNS3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTPAIHAAT AARVGHQPAP SFEAAAQRFD IIPLDAPVGA EVVGIDLSQP LDAAAFARIH RAHLDHHVLV FRDQRITPEQ HIAFSRRFGP LQIHVLHQFA LAGHPEVLIV SNIVENGKPI GLGDAGHFWH SDLSYKEKPS LGSLLHAQEL PADGGDTLFA NMHLAWDTLP AHLRRAVEGR RAEHTYLARY AELQARNRWR PVLSAEQLAQ VEAVVHPIVR THPETGRKAL FVSEHFTTRI VDLPADESRA LLDELFAHSV RDEHLYRHRW RDHDLVFWDN RSLMHLAAGT PDHLRRKLYR TTIEGDAPF
NT Sequence
GTGACGACTC CCGCCATCCA CGCCGCAACA GCGGCGCGCG TCGGCCATCA GCCTGCACCG TCGTTCGAGG CCGCCGCGCA GCGGTTCGAC ATCATCCCGC TCGATGCGCC GGTCGGCGCA GAAGTCGTCG GGATCGACCT GTCGCAACCG CTGGACGCGG CCGCTTTCGC ACGCATCCAT CGCGCGCATC TCGACCATCA CGTGCTGGTG TTTCGCGACC AGCGCATCAC GCCGGAACAG CACATCGCGT TCAGCCGCCG CTTCGGCCCG CTGCAGATCC ACGTGCTGCA CCAATTCGCG CTGGCCGGGC ATCCGGAAGT GCTGATCGTG TCGAACATCG TCGAGAACGG CAAGCCGATC GGGCTCGGCG ACGCCGGCCA TTTCTGGCAC TCCGATCTGT CGTACAAGGA AAAACCGAGC CTCGGCTCGC TGCTGCATGC GCAGGAACTG CCGGCCGACG GCGGCGACAC GCTGTTCGCC AACATGCATC TCGCATGGGA TACGCTGCCG GCGCACCTGC GTCGCGCGGT CGAAGGGCGG CGCGCCGAAC ATACGTATCT CGCGCGCTAT GCGGAGCTGC AGGCACGCAA CCGGTGGCGG CCCGTGCTGT CGGCCGAGCA GCTTGCGCAA GTCGAAGCGG TGGTGCACCC GATCGTGCGC ACGCATCCGG AGACGGGCCG CAAGGCGCTG TTCGTGAGCG AGCATTTCAC GACACGCATC GTCGACCTGC CCGCCGACGA AAGCCGCGCA CTGCTCGACG AACTGTTCGC GCACAGCGTG CGGGACGAGC ACCTGTATCG CCATCGGTGG CGCGATCACG ACCTCGTGTT CTGGGACAAC CGCTCGCTGA TGCATCTCGC GGCCGGCACG CCGGACCATC TGCGCCGCAA GCTGTACCGC ACGACGATCG AAGGCGACGC GCCGTTC
Details for BuviA.00024.a.B1.GE38077
HARVESTED ON: 5/13/2014
SEQUENCED ON: 5/6/2014
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TTPAIHAATA ARVGHQPAPS FEAAAQRFDI IPLDAPVGAE VVGIDLSQPL DAAAFARIHR AHLDHHVLVF RDQRITPEQH IAFSRRFGPL QIHVLHQFAL AGHPEVLIVS NIVENGKPIG LGDAGHFWHS DLSYKEKPSL GSLLHAQELP ADGGDTLFAN MHLAWDTLPA HLRRAVEGRR AEHTYLARYA ELQARNRWRP VLSAEQLAQV EAVVHPIVRT HPETGRKALF VSEHFTTRIV DLPADESRAL LDELFAHSVR DEHLYRHRWR DHDLVFWDNR SLMHLAAGTP DHLRRKLYRT TIEGXAPF
Validated NT Sequence
ttcctctana ntaattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgacgact cccgccatcc acgccgcaac agcggcgcgc gtcggccatc agcctgcacc gtcgttcgag gccgccgcgc agcggttcga catcatcccg ctcgatgcgc cggtcggcgc agaagtcgtc gggatcgacc tgtcgcaacc gctggacgcg gccgctttcg cacgcatcca tcgcgcgcat ctcgaccatc acgtgctggt gtttcgcgac cagcgcatca cgccggaaca gcacatcgcg ttcagccgcc gcttcggccc gctgcagatc cacgtgctgc accaattcgc gctggccggg catccggaag tgctgatcgt gtcgaacatc gtcgagaacg gcaagccgat cgggctcggc gacgccggcc atttctggca ctccgatctg tcgtacaagg aaaaaccgag cctcggctcg ctgctgcatg cgcaggaact gccggccgac ggcggcgaca cgctgttcgc caacatgcat ctcgcatggg atacgctgcc ggcgcacctg cgtcgcgcgg tcgaagggcg gcgcgccgaa catacgtatc tcgcgcgcta tgcggagctg caggcacgca accggtggcg gcccgtgctg tcggccgagc agcttgcgca agtcgaagcg gtggtgcacc cgatcgtgcg cacgcatccg gagacgggcc gcaaggcgct gttcgtgagc gagcatttca cgacacgcat cgtcgacctg cccgccgacg aaagccgcgc actgctcgac gaactgttcg cgcacagcgt gcgggacgag cacctgtatc gccatcggtg gcgcgatcac gacctcgtgt tctgggacaa ccgctcgctg atgcatctcg cggccggcac gccggaccat ctgcgccgca agctgtaccg cacgacgatc gaaggnnacg cgccgttcta agtgagtaga taggatccgg ctgctannna nncccccaag aga
Expected Protein Sequence
MAHHHHHHMT TPAIHAATAA RVGHQPAPSF EAAAQRFDII PLDAPVGAEV VGIDLSQPLD AAAFARIHRA HLDHHVLVFR DQRITPEQHI AFSRRFGPLQ IHVLHQFALA GHPEVLIVSN IVENGKPIGL GDAGHFWHSD LSYKEKPSLG SLLHAQELPA DGGDTLFANM HLAWDTLPAH LRRAVEGRRA EHTYLARYAE LQARNRWRPV LSAEQLAQVE AVVHPIVRTH PETGRKALFV SEHFTTRIVD LPADESRALL DELFAHSVRD EHLYRHRWRD HDLVFWDNRS LMHLAAGTPD HLRRKLYRTT IEGDAPF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacga ctcccgccat ccacgccgca acagcggcgc gcgtcggcca tcagcctgca ccgtcgttcg aggccgccgc gcagcggttc gacatcatcc cgctcgatgc gccggtcggc gcagaagtcg tcgggatcga cctgtcgcaa ccgctggacg cggccgcttt cgcacgcatc catcgcgcgc atctcgacca tcacgtgctg gtgtttcgcg accagcgcat cacgccggaa cagcacatcg cgttcagccg ccgcttcggc ccgctgcaga tccacgtgct gcaccaattc gcgctggccg ggcatccgga agtgctgatc gtgtcgaaca tcgtcgagaa cggcaagccg atcgggctcg gcgacgccgg ccatttctgg cactccgatc tgtcgtacaa ggaaaaaccg agcctcggct cgctgctgca tgcgcaggaa ctgccggccg acggcggcga cacgctgttc gccaacatgc atctcgcatg ggatacgctg ccggcgcacc tgcgtcgcgc ggtcgaaggg cggcgcgccg aacatacgta tctcgcgcgc tatgcggagc tgcaggcacg caaccggtgg cggcccgtgc tgtcggccga gcagcttgcg caagtcgaag cggtggtgca cccgatcgtg cgcacgcatc cggagacggg ccgcaaggcg ctgttcgtga gcgagcattt cacgacacgc atcgtcgacc tgcccgccga cgaaagccgc gcactgctcg acgaactgtt cgcgcacagc gtgcgggacg agcacctgta tcgccatcgg tggcgcgatc acgacctcgt gttctgggac aaccgctcgc tgatgcatct cgcggccggc acgccggacc atctgcgccg caagctgtac cgcacgacga tcgaaggcga cgcgccgttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat