SSGCID: Target Page

BuviA.00020.x

Aldehyde dehydrogenase

CENTER ID:	BuviA.00020.x
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00020.x.B1.GE39407	Full length( BuviA.00020.x )		1	473

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.1620
RefSeq:	YP_001115421.1
UniProt:	A4JSB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRPSRPPELA ASFERQRSAY ARDPAPPLAI RLSRLDRLRA LLDTHEAALV QAIDADFGGR SAHETRLAEL FVVHSGLRHA RAHLRQWMRE RRVPTAAHFR PGYNRIAPQP RGVVGVMAPW NYPLQLALGP AVAALAAGNR VLVKPSDLTP RLSALLESLV ADAFDVDELA VVTGDVDTAK AFARLPFDHL FFTGSTAVGR DVAQAAAANL TPVTLELGGK SPAIVDPSCD LAHAAARIAF GKLLNAGQTC IAPDYLLVPA GTAETVAARV ADAIAHHYPT LAANPDYTSI IAATPRDRLA ALVDDARAGG ARVREVNPAG ERFAAGSRKF APTLVLDARD TMRVMREEIF GPVLPIVEYA DLDDAIDRVN RGPRPLALYW FGRDAARRDR VLRETVSGGV TVNDCLWHLA QEHQPFGGVG ASGIGAYHGK WGFDTFSHHK PVFHQARRSG TALFHPPYGK TFDLLLRVLT RFA

NT Sequence

ATGCGACCGA GTCGCCCACC CGAGCTCGCC GCCAGCTTCG AACGCCAGCG CTCCGCCTAT GCGCGCGATC CCGCGCCACC GCTCGCGATC CGCCTGTCGC GGCTCGACCG TCTGCGCGCG CTGCTCGATA CGCATGAAGC CGCGCTCGTG CAGGCGATCG ACGCCGATTT CGGCGGACGC TCCGCGCACG AGACGCGGCT CGCCGAACTG TTCGTGGTCC ATAGCGGCCT GCGCCATGCG CGCGCGCACC TGCGGCAGTG GATGCGCGAG CGCCGCGTGC CGACCGCCGC GCATTTCCGG CCGGGCTACA ACCGGATCGC GCCGCAACCG CGCGGGGTCG TCGGCGTGAT GGCGCCGTGG AACTATCCGC TGCAGCTCGC GCTCGGGCCG GCGGTCGCCG CGCTCGCCGC CGGCAACCGC GTGCTGGTCA AGCCGTCCGA CCTGACGCCG CGGCTGTCGG CCCTGCTCGA ATCGCTCGTC GCCGACGCGT TCGATGTGGA CGAACTCGCC GTCGTCACCG GCGACGTCGA CACCGCGAAG GCGTTCGCGC GCCTGCCGTT CGACCATCTG TTCTTCACCG GCTCGACCGC GGTCGGGCGC GACGTCGCGC AGGCCGCGGC CGCGAACCTG ACGCCGGTCA CGCTGGAACT GGGCGGCAAG TCGCCGGCGA TCGTCGACCC GTCGTGCGAT CTGGCGCACG CGGCCGCGCG CATCGCGTTC GGCAAGCTGC TCAACGCCGG CCAGACCTGC ATCGCGCCCG ACTATCTGCT GGTGCCGGCC GGCACCGCCG AGACCGTCGC GGCACGCGTG GCGGACGCGA TCGCGCATCA CTACCCGACG CTCGCCGCCA ATCCCGACTA CACGTCGATC ATCGCCGCCA CGCCTCGCGA CCGCCTCGCG GCGCTCGTCG ACGACGCGCG CGCCGGCGGC GCACGCGTGC GCGAAGTGAA CCCGGCAGGC GAACGCTTCG CTGCCGGCTC GCGCAAGTTC GCGCCGACCC TCGTGCTGGA CGCGCGCGAC ACGATGCGCG TGATGCGCGA GGAGATCTTC GGCCCGGTGC TGCCGATCGT CGAATACGCG GATCTCGACG ATGCGATCGA CCGCGTGAAC CGCGGCCCGC GCCCGCTCGC GCTGTACTGG TTCGGCCGCG ACGCCGCGCG GCGCGATCGC GTGCTGCGCG AGACGGTGTC GGGCGGCGTC ACGGTGAACG ACTGCCTGTG GCATCTCGCG CAGGAGCACC AGCCGTTCGG CGGCGTCGGC GCGAGCGGCA TCGGCGCCTA TCACGGCAAA TGGGGCTTCG ATACGTTCAG CCACCACAAG CCCGTGTTCC ATCAGGCGCG CCGCAGCGGC ACCGCGCTGT TCCATCCGCC GTACGGCAAG ACCTTCGATC TGCTGCTGCG CGTGCTGACG CGCTTCGCG

Details for BuviA.00020.x.B1.GE39407

HARVESTED ON:	8/5/2015
SEQUENCED ON:	8/13/2015
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR PSRPPELAAS FERQRSAYAR DPAPPLAIRL SRLDRLRALL DTHEAALVQA IDADFGGRSA HETRLAELFV VHSGLRHARA HLRQWMRERR VPTAAHFRPG YNRIAPQPRG VVGVMAPWNY PLQLALGPAV AALAAGNRVL VKPSDLTPRL SALLESLVAD AFDVDELAVV TGDVDTAKAF ARLPFDHLFF TGSTAVGRDV AQAAAANLTP VTLELGGKSP AIVDPSCDLA HAAARIAFGK LLNAGQTCIA PDYLLVPAGT AETVAARVAD AIAHHYPTLA ANPDYTSIIA ATPRDRLAAL VDDARAGGAR VREVNPAGER FAAGSRKFAP TLVLDARDTM RVMREEIFGP VLPIVEYADL DDAIDRVNRG PRPLALYWFG RDAARRDRVL RETVSGGVTV NDCLWHLAQE HQPFGGVGAS GIGAYHGKWG FDTFSHHKPV FHQARRSGTA LFHPPYGKTF DL

Validated NT Sequence

gcagatcgaa ggtcttgccg tacggcggat ggaacagcgc ggtgccgctg cggcgcgcct gatggaacac gggcttgtgg tggctgaacg tatcgaagcc ccatttgccg tgataggcgc cgatgccgct cgcgccgacg ccgccgaacg gctggtgctc ctgcgcgaga tgccacaggc agtcgttcac cgtgacgccg cccgacaccg tctcgcgcag cacgcgatcg cgccgcgcgg cgtcgcggcc gaaccagtac agcgcgagcg ggcgcgggcc gcggttcacg cggtcgatcg catcgtcgag atccgcgtat tcgacgatcg gcagcaccgg gccgaagatc tcctcgcgca tcacgcgcat cgtgtcgcgc gcgtccagca cgagggtcgg cgcgaacttg cgcgagccgg cagcgaagcg ttcgcctgcc gggttcactt cgcgcacgcg tgcgccgccg gcgcgcgcgt cgtcgacgag cgccgcgagg cggtcgcgag gcgtggcggc gatgatcgac gtgtagtcgg gattggcggc gagcgtcggg tagtgatgcg cgatcgcgtc cgccacgcgt gccgcgacgg tctcggcggt gccggccggc accagcagat agtcgggcgc gatgcaggtc tggccggcgt tgagcagctt gccgaacgcg atgcgcgcgg ccgcgtgcgc cagatcgcac gacgggtcga cgatcgccgg cgacttgccg cccagttcca gcgtgaccgg cgtcaggttc gcggccgcgg cctgcgcgac gtcgcgcccg accgcggtcg agccggtgaa gaacagatgg tcgaacggca ggcgcgcgaa cgccttcgcg gtgtcgacgt cgccggtgac gacggcgagt tcgtccacat cgaacgcgtc ggcgacgagc gattcgagca gggccgacag ccgcggcgtc aggtcggacg gcttgaccag cacgcggttg ccggcggcga gcgcggcgac cgccggcccg agcgcgagct gcagcggata gttccacggc gccatcacgc cgacgacccc gcgcggttgc ggcgcgatcc ggttgtagcc cggccggaaa tgcgcggcgg tcggcacgcg gcgctcgcgc atccactgcc gcaggtgcgc gcgcgcatgg cgcaggccgc tatggaccac gaacagttcg gcgagccgcg tctcgtgcgc ggagcgtccg ccgaaatcgg cgtcgatcgc ctgcacgagc gcggcttcat gcgtatcgag cagcgcgcgc agacggtcga gccgcgacag gcggatcgcg agcggtggcg cgggatcgcg cgcataggcg gagcgctggc gttcgaagct ggcggcgagc tcgggtgggc gactcggtcg catatggtgg tggtggtggt gagccatggt atat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgac cgagtcgccc acccgagctc gccgccagct tcgaacgcca gcgctccgcc tatgcgcgcg atcccgcgcc accgctcgcg atccgcctgt cgcggctcga ccgtctgcgc gcgctgctcg atacgcatga agccgcgctc gtgcaggcga tcgacgccga tttcggcgga cgctccgcgc acgagacgcg gctcgccgaa ctgttcgtgg tccatagcgg cctgcgccat gcgcgcgcgc acctgcggca gtggatgcgc gagcgccgcg tgccgaccgc cgcgcatttc cggccgggct acaaccggat cgcgccgcaa ccgcgcgggg tcgtcggcgt gatggcgccg tggaactatc cgctgcagct cgcgctcggg ccggcggtcg ccgcgctcgc cgccggcaac cgcgtgctgg tcaagccgtc cgacctgacg ccgcggctgt cggccctgct cgaatcgctc gtcgccgacg cgttcgatgt ggacgaactc gccgtcgtca ccggcgacgt cgacaccgcg aaggcgttcg cgcgcctgcc gttcgaccat ctgttcttca ccggctcgac cgcggtcggg cgcgacgtcg cgcaggccgc ggccgcgaac ctgacgccgg tcacgctgga actgggcggc aagtcgccgg cgatcgtcga cccgtcgtgc gatctggcgc acgcggccgc gcgcatcgcg ttcggcaagc tgctcaacgc cggccagacc tgcatcgcgc ccgactatct gctggtgccg gccggcaccg ccgagaccgt cgcggcacgc gtggcggacg cgatcgcgca tcactacccg acgctcgccg ccaatcccga ctacacgtcg atcatcgccg ccacgcctcg cgaccgcctc gcggcgctcg tcgacgacgc gcgcgccggc ggcgcacgcg tgcgcgaagt gaacccggca ggcgaacgct tcgctgccgg ctcgcgcaag ttcgcgccga ccctcgtgct ggacgcgcgc gacacgatgc gcgtgatgcg cgaggagatc ttcggcccgg tgctgccgat cgtcgaatac gcggatctcg acgatgcgat cgaccgcgtg aaccgcggcc cgcgcccgct cgcgctgtac tggttcggcc gcgacgccgc gcggcgcgat cgcgtgctgc gcgagacggt gtcgggcggc gtcacggtga acgactgcct gtggcatctc gcgcaggagc accagccgtt cggcggcgtc ggcgcgagcg gcatcggcgc ctatcacggc aaatggggct tcgatacgtt cagccaccac aagcccgtgt tccatcaggc gcgccgcagc ggcaccgcgc tgttccatcc gccgtacggc aagaccttcg atctgctgct gcgcgtgctg acgcgcttcg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat