BuviA.00020.o
Aldehyde dehydrogenase
| CENTER ID: | BuviA.00020.o |
| ORGANISM: | Burkholderia vietnamiensis G4 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00020.o.B1.GE39405 | Full length( BuviA.00020.o ) | 1 | 477 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuviA.00020.o.B1.PS37818 | Full length( BuviA.00020.o ) | 1 | 477 |
Structures
5KF0
DEPOSITED: 6/10/2016
DETERMINATION: XRay
CLONE: BuviA.00020.o.B1.GE39405
PROTEIN: BuviA.00020.o.B1.PS37818
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|269482.11.peg.2910 |
| RefSeq: | YP_001116615.1 |
| UniProt: | A4JLJ7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKDTYPYYL ANEAVYANTD LEVTDKFSGK VATRVALADA QAIDAAIGAA VDAVKPMREL PAYRRQAVLD HCVARFRERF DELAEALCIE AGKPINDSRG EVTRLIDTFR VASEEAVRID GEVLNLEISA RAQGYTGYTR RVPIGPCSFI SPFNFPLNLA AHKVAPALAA GCPFVLKPAS RTPVGALIIA EVLAETNLPK GAFSVLPAHR DGADLFTTDE RFRLLSFTGS PAVGWALKEK AGKKKVVLEL GGNAAAIVDA DQFEQLDYVV DRLAFGAYYQ SGQSCIGVQR ILVHASLYDT LRDKLVAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI IAGGKVDGAM FEATLLEDVG REQDLYRKEA FGPVAILEKF DRFDDALARV NDSDFGLQAG VFTDSLTHAQ RAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGREGIR YAIEDMTEPR LMVVRRR
NT Sequence
ATGCTAAAGG ATACCTATCC GTACTATCTG GCCAACGAGG CCGTCTACGC GAATACCGAT CTGGAAGTGA CGGACAAGTT CAGCGGCAAG GTCGCGACGC GCGTCGCGCT GGCCGACGCG CAGGCGATCG ACGCGGCCAT CGGCGCGGCA GTCGACGCCG TCAAGCCGAT GCGCGAGCTG CCGGCGTACC GGCGGCAGGC CGTGCTCGAT CACTGCGTTG CACGCTTTCG CGAGCGTTTC GACGAGCTGG CCGAGGCGCT GTGCATCGAG GCCGGCAAGC CGATCAACGA TTCGAGAGGC GAGGTGACGC GGCTGATCGA TACGTTCCGC GTCGCGTCCG AGGAGGCGGT GCGCATCGAC GGCGAAGTGC TCAACCTCGA GATCTCGGCG CGCGCGCAGG GCTACACCGG CTATACGCGG CGCGTGCCGA TCGGCCCGTG CTCGTTCATC TCGCCGTTCA ACTTCCCGCT GAACCTCGCC GCGCACAAGG TCGCGCCCGC GCTCGCCGCG GGCTGCCCGT TCGTGCTCAA ACCGGCGAGC CGCACGCCGG TCGGCGCGCT GATCATCGCC GAGGTGCTCG CGGAAACCAA TCTGCCGAAG GGCGCGTTCT CGGTGCTGCC CGCGCATCGC GACGGCGCCG ACCTGTTCAC CACCGACGAG CGTTTCAGGT TGCTGTCGTT CACCGGCTCG CCGGCCGTCG GCTGGGCGCT GAAGGAGAAG GCCGGCAAGA AGAAGGTCGT GCTGGAGCTC GGCGGCAACG CGGCGGCCAT CGTCGACGCG GACCAGTTCG AGCAGCTCGA CTACGTGGTC GACCGGCTCG CGTTCGGCGC GTATTACCAG TCGGGCCAGA GCTGCATCGG CGTGCAGCGG ATCCTCGTGC ATGCGAGCCT GTACGACACG TTGCGCGACA AGCTGGTCGC GAAGACGCGC TCGCTGAAGA TGGGCGATCC GAAGGATCCA TCGACGTTCG TCGGCCCGAT GATCTCCGAA TCGGAGTCGC GCCGCCTGTC CGGCTGGATG GACGCGGCCG TCGCGGCGGG TGCGAAGATC ATCGCGGGCG GCAAGGTCGA CGGCGCGATG TTCGAGGCGA CGCTGCTCGA AGACGTCGGC CGCGAGCAGG ACCTGTACCG CAAGGAGGCG TTCGGCCCGG TCGCGATCCT CGAGAAGTTC GATCGTTTCG ACGATGCGCT CGCGCGCGTG AACGACAGCG ACTTCGGCTT GCAGGCCGGC GTGTTTACCG ATTCGCTCAC GCACGCGCAG CGCGCGTGGG ACGAGCTCGA GGTGGGGGGC GTCGTGATCA ACGACGTGCC GTCGTTCCGC GTCGACAACA TGCCGTACGG CGGCGTGAAG GATTCCGGCC TCGGCCGCGA AGGGATCCGC TACGCGATCG AGGACATGAC CGAGCCGCGT CTGATGGTCG TTCGGCGCCG A
Details for BuviA.00020.o.B1.GE39405
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHHH MLKDTYPYYL ANEAVYANTD LEVTDKFSGK VATRVALADA QAIDAAIGAA VDAVKPMREL PAYRRQAVLD HCVARFRERF DELAEALCIE AGKPINDSRG EVTRLIDTFR VASEEAVRID GEVLNLEISA RAQGYTGYTR RVPIGPCSFI SPFNFPLNLA AHKVAPALAA GCPFVLKPAS RTPVGALIIA EVLAETNLPK GAFSVLPAHR DGADLFTTDE RFRLLSFTGS PAVGWALKEK AGKKKVVLEL GGNAAAIVDA DQFEQLDYVV DRLAFGAYYQ SGQSCIGVQR ILVHASLYDT LRDKLVAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI IAGGKVDGAM FEATLLEDVG REQDLYRKEA FGPVAILEKF DRFDDALARV NDSDFGLQAG VFTDSLTHAQ RAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGXXGIR YAIEDMT
Validated NT Sequence
cggtcatgtc ctcgatcgcg tagcggatcc cnnnnnnncc gaggccggaa tccttcacgc cgccgtacgg catgttgtcg acgcggaacg acggcacgtc gttgatcacg acgcccccca cctcgagctc gtcccacgcg cgctgcgcgt gcgtgagcga atcggtaaac acgccggcct gcaagccgaa gtcgctgtcg ttcacgcgcg cgagcgcatc gtcgaaacga tcgaacttct cgaggatcgc gaccgggccg aacgcctcct tgcggtacag gtcctgctcg cggccgacgt cttcgagcag cgtcgcctcg aacatcgcgc cgtcgacctt gccgcccgcg atgatcttcg cacccgccgc gacggccgcg tccatccagc cggacaggcg gcgcgactcc gattcggaga tcatcgggcc gacgaacgtc gatggatcct tcggatcgcc catcttcagc gagcgcgtct tcgcgaccag cttgtcgcgc aacgtgtcgt acaggctcgc atgcacgagg atccgctgca cgccgatgca gctctggccc gactggtaat acgcgccgaa cgcgagccgg tcgaccacgt agtcgagctg ctcgaactgg tccgcgtcga cgatggccgc cgcgttgccg ccgagctcca gcacgacctt cttcttgccg gccttctcct tcagcgccca gccgacggcc ggcgagccgg tgaacgacag caacctgaaa cgctcgtcgg tggtgaacag gtcggcgccg tcgcgatgcg cgggcagcac cgagaacgcg cccttcggca gattggtttc cgcgagcacc tcggcgatga tcagcgcgcc gaccggcgtg cggctcgccg gtttgagcac gaacgggcag cccgcggcga gcgcgggcgc gaccttgtgc gcggcgaggt tcagcgggaa gttgaacggc gagatgaacg agcacgggcc gatcggcacg cgccgcgtat agccggtgta gccctgcgcg cgcgccgaga tctcgaggtt gagcacttcg ccgtcgatgc gcaccgcctc ctcggacgcg acgcggaacg tatcgatcag ccgcgtcacc tcgcctctcg aatcgttgat cggcttgccg gcctcgatgc acagcgcctc ggccagctcg tcgaaacgct cgcgaaagcg tgcaacgcag tgatcgagca cggcctgccg ccggtacgcc ggcagctcgc gcatcggctt gacggcgtcg actgccgcgc cgatggccgc gtcgatcgcc tgcgcgtcgg ccagcgcgac gcgcgtcgcg accttgccgc tgaacttgtc cgtcacttcc agatcggtat tcgcgtagac ggcctcgttg gccagatagt acggataggt atcctttagc atatggtggt ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHML KDTYPYYLAN EAVYANTDLE VTDKFSGKVA TRVALADAQA IDAAIGAAVD AVKPMRELPA YRRQAVLDHC VARFRERFDE LAEALCIEAG KPINDSRGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH KVAPALAAGC PFVLKPASRT PVGALIIAEV LAETNLPKGA FSVLPAHRDG ADLFTTDERF RLLSFTGSPA VGWALKEKAG KKKVVLELGG NAAAIVDADQ FEQLDYVVDR LAFGAYYQSG QSCIGVQRIL VHASLYDTLR DKLVAKTRSL KMGDPKDPST FVGPMISESE SRRLSGWMDA AVAAGAKIIA GGKVDGAMFE ATLLEDVGRE QDLYRKEAFG PVAILEKFDR FDDALARVND SDFGLQAGVF TDSLTHAQRA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLM VVRRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctaa aggataccta tccgtactat ctggccaacg aggccgtcta cgcgaatacc gatctggaag tgacggacaa gttcagcggc aaggtcgcga cgcgcgtcgc gctggccgac gcgcaggcga tcgacgcggc catcggcgcg gcagtcgacg ccgtcaagcc gatgcgcgag ctgccggcgt accggcggca ggccgtgctc gatcactgcg ttgcacgctt tcgcgagcgt ttcgacgagc tggccgaggc gctgtgcatc gaggccggca agccgatcaa cgattcgaga ggcgaggtga cgcggctgat cgatacgttc cgcgtcgcgt ccgaggaggc ggtgcgcatc gacggcgaag tgctcaacct cgagatctcg gcgcgcgcgc agggctacac cggctatacg cggcgcgtgc cgatcggccc gtgctcgttc atctcgccgt tcaacttccc gctgaacctc gccgcgcaca aggtcgcgcc cgcgctcgcc gcgggctgcc cgttcgtgct caaaccggcg agccgcacgc cggtcggcgc gctgatcatc gccgaggtgc tcgcggaaac caatctgccg aagggcgcgt tctcggtgct gcccgcgcat cgcgacggcg ccgacctgtt caccaccgac gagcgtttca ggttgctgtc gttcaccggc tcgccggccg tcggctgggc gctgaaggag aaggccggca agaagaaggt cgtgctggag ctcggcggca acgcggcggc catcgtcgac gcggaccagt tcgagcagct cgactacgtg gtcgaccggc tcgcgttcgg cgcgtattac cagtcgggcc agagctgcat cggcgtgcag cggatcctcg tgcatgcgag cctgtacgac acgttgcgcg acaagctggt cgcgaagacg cgctcgctga agatgggcga tccgaaggat ccatcgacgt tcgtcggccc gatgatctcc gaatcggagt cgcgccgcct gtccggctgg atggacgcgg ccgtcgcggc gggtgcgaag atcatcgcgg gcggcaaggt cgacggcgcg atgttcgagg cgacgctgct cgaagacgtc ggccgcgagc aggacctgta ccgcaaggag gcgttcggcc cggtcgcgat cctcgagaag ttcgatcgtt tcgacgatgc gctcgcgcgc gtgaacgaca gcgacttcgg cttgcaggcc ggcgtgttta ccgattcgct cacgcacgcg cagcgcgcgt gggacgagct cgaggtgggg ggcgtcgtga tcaacgacgt gccgtcgttc cgcgtcgaca acatgccgta cggcggcgtg aaggattccg gcctcggccg cgaagggatc cgctacgcga tcgaggacat gaccgagccg cgtctgatgg tcgttcggcg ccgatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuviA.00020.o.B1.PS37818
| PURIFICATION DATe: | 1/19/2016 |
| CONCENTRATION: | 37.1mg/ml |
| OBSERVED MW: | 53kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHH MLKDTYPYYL ANEAVYANTD LEVTDKFSGK VATRVALADA QAIDAAIGAA VDAVKPMREL PAYRRQAVLD HCVARFRERF DELAEALCIE AGKPINDSRG EVTRLIDTFR VASEEAVRID GEVLNLEISA RAQGYTGYTR RVPIGPCSFI SPFNFPLNLA AHKVAPALAA GCPFVLKPAS RTPVGALIIA EVLAETNLPK GAFSVLPAHR DGADLFTTDE RFRLLSFTGS PAVGWALKEK AGKKKVVLEL GGNAAAIVDA DQFEQLDYVV DRLAFGAYYQ SGQSCIGVQR ILVHASLYDT LRDKLVAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI IAGGKVDGAM FEATLLEDVG REQDLYRKEA FGPVAILEKF DRFDDALARV NDSDFGLQAG VFTDSLTHAQ RAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGXXGIR YAIEDMT
Validated NT Sequence
cggtcatgtc ctcgatcgcg tagcggatcc cnnnnnnncc gaggccggaa tccttcacgc cgccgtacgg catgttgtcg acgcggaacg acggcacgtc gttgatcacg acgcccccca cctcgagctc gtcccacgcg cgctgcgcgt gcgtgagcga atcggtaaac acgccggcct gcaagccgaa gtcgctgtcg ttcacgcgcg cgagcgcatc gtcgaaacga tcgaacttct cgaggatcgc gaccgggccg aacgcctcct tgcggtacag gtcctgctcg cggccgacgt cttcgagcag cgtcgcctcg aacatcgcgc cgtcgacctt gccgcccgcg atgatcttcg cacccgccgc gacggccgcg tccatccagc cggacaggcg gcgcgactcc gattcggaga tcatcgggcc gacgaacgtc gatggatcct tcggatcgcc catcttcagc gagcgcgtct tcgcgaccag cttgtcgcgc aacgtgtcgt acaggctcgc atgcacgagg atccgctgca cgccgatgca gctctggccc gactggtaat acgcgccgaa cgcgagccgg tcgaccacgt agtcgagctg ctcgaactgg tccgcgtcga cgatggccgc cgcgttgccg ccgagctcca gcacgacctt cttcttgccg gccttctcct tcagcgccca gccgacggcc ggcgagccgg tgaacgacag caacctgaaa cgctcgtcgg tggtgaacag gtcggcgccg tcgcgatgcg cgggcagcac cgagaacgcg cccttcggca gattggtttc cgcgagcacc tcggcgatga tcagcgcgcc gaccggcgtg cggctcgccg gtttgagcac gaacgggcag cccgcggcga gcgcgggcgc gaccttgtgc gcggcgaggt tcagcgggaa gttgaacggc gagatgaacg agcacgggcc gatcggcacg cgccgcgtat agccggtgta gccctgcgcg cgcgccgaga tctcgaggtt gagcacttcg ccgtcgatgc gcaccgcctc ctcggacgcg acgcggaacg tatcgatcag ccgcgtcacc tcgcctctcg aatcgttgat cggcttgccg gcctcgatgc acagcgcctc ggccagctcg tcgaaacgct cgcgaaagcg tgcaacgcag tgatcgagca cggcctgccg ccggtacgcc ggcagctcgc gcatcggctt gacggcgtcg actgccgcgc cgatggccgc gtcgatcgcc tgcgcgtcgg ccagcgcgac gcgcgtcgcg accttgccgc tgaacttgtc cgtcacttcc agatcggtat tcgcgtagac ggcctcgttg gccagatagt acggataggt atcctttagc atatggtggt ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHML KDTYPYYLAN EAVYANTDLE VTDKFSGKVA TRVALADAQA IDAAIGAAVD AVKPMRELPA YRRQAVLDHC VARFRERFDE LAEALCIEAG KPINDSRGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH KVAPALAAGC PFVLKPASRT PVGALIIAEV LAETNLPKGA FSVLPAHRDG ADLFTTDERF RLLSFTGSPA VGWALKEKAG KKKVVLELGG NAAAIVDADQ FEQLDYVVDR LAFGAYYQSG QSCIGVQRIL VHASLYDTLR DKLVAKTRSL KMGDPKDPST FVGPMISESE SRRLSGWMDA AVAAGAKIIA GGKVDGAMFE ATLLEDVGRE QDLYRKEAFG PVAILEKFDR FDDALARVND SDFGLQAGVF TDSLTHAQRA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLM VVRRR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTAA AGGATACCTA TCCGTACTAT CTGGCCAACG AGGCCGTCTA CGCGAATACC GATCTGGAAG TGACGGACAA GTTCAGCGGC AAGGTCGCGA CGCGCGTCGC GCTGGCCGAC GCGCAGGCGA TCGACGCGGC CATCGGCGCG GCAGTCGACG CCGTCAAGCC GATGCGCGAG CTGCCGGCGT ACCGGCGGCA GGCCGTGCTC GATCACTGCG TTGCACGCTT TCGCGAGCGT TTCGACGAGC TGGCCGAGGC GCTGTGCATC GAGGCCGGCA AGCCGATCAA CGATTCGAGA GGCGAGGTGA CGCGGCTGAT CGATACGTTC CGCGTCGCGT CCGAGGAGGC GGTGCGCATC GACGGCGAAG TGCTCAACCT CGAGATCTCG GCGCGCGCGC AGGGCTACAC CGGCTATACG CGGCGCGTGC CGATCGGCCC GTGCTCGTTC ATCTCGCCGT TCAACTTCCC GCTGAACCTC GCCGCGCACA AGGTCGCGCC CGCGCTCGCC GCGGGCTGCC CGTTCGTGCT CAAACCGGCG AGCCGCACGC CGGTCGGCGC GCTGATCATC GCCGAGGTGC TCGCGGAAAC CAATCTGCCG AAGGGCGCGT TCTCGGTGCT GCCCGCGCAT CGCGACGGCG CCGACCTGTT CACCACCGAC GAGCGTTTCA GGTTGCTGTC GTTCACCGGC TCGCCGGCCG TCGGCTGGGC GCTGAAGGAG AAGGCCGGCA AGAAGAAGGT CGTGCTGGAG CTCGGCGGCA ACGCGGCGGC CATCGTCGAC GCGGACCAGT TCGAGCAGCT CGACTACGTG GTCGACCGGC TCGCGTTCGG CGCGTATTAC CAGTCGGGCC AGAGCTGCAT CGGCGTGCAG CGGATCCTCG TGCATGCGAG CCTGTACGAC ACGTTGCGCG ACAAGCTGGT CGCGAAGACG CGCTCGCTGA AGATGGGCGA TCCGAAGGAT CCATCGACGT TCGTCGGCCC GATGATCTCC GAATCGGAGT CGCGCCGCCT GTCCGGCTGG ATGGACGCGG CCGTCGCGGC GGGTGCGAAG ATCATCGCGG GCGGCAAGGT CGACGGCGCG ATGTTCGAGG CGACGCTGCT CGAAGACGTC GGCCGCGAGC AGGACCTGTA CCGCAAGGAG GCGTTCGGCC CGGTCGCGAT CCTCGAGAAG TTCGATCGTT TCGACGATGC GCTCGCGCGC GTGAACGACA GCGACTTCGG CTTGCAGGCC GGCGTGTTTA CCGATTCGCT CACGCACGCG CAGCGCGCGT GGGACGAGCT CGAGGTGGGG GGCGTCGTGA TCAACGACGT GCCGTCGTTC CGCGTCGACA ACATGCCGTA CGGCGGCGTG AAGGATTCCG GCCTCGGCCG CGAAGGGATC CGCTACGCGA TCGAGGACAT GACCGAGCCG CGTCTGATGG TCGTTCGGCG CCGATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT