SSGCID: Target Page

BuviA.00013.a

Holliday junction ATP-dependent DNA helicase ruvB

CENTER ID:	BuviA.00013.a
ORGANISM:	Burkholderia vietnamiensis G4
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuviA.00013.a.B1.GE38075	Full length( BuviA.00013.a )		1	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|269482.11.peg.4858
RefSeq:	YP_001118500.1
UniProt:	A4JBL2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIETDKLAAE RIIAATPASS HEEVFERALR PRQLDDYVGQ EKVRGQLEIF IEAAKRRSEP LDHVLLFGPP GLGKTTLAHI IAREMGVNLR QTSGPVLERA GDLAALLTNL EANDVLFIDE IHRLSPVVEE ILYPALEDYQ IDIMIGEGPA ARSVKLDLQP FTLVGATTRA GMLTNPLRDR FGIVARLEFY DAEQLSRIVR RSAALLNAQI DPNGALEIAK RSRGTPRIAN RLLRRVRDYA EVKADGQITA AVADAALAML DVDPVGFDLM DRKLLEAILH KFDGGPVGID NLAAAIGEER DTIEDVLEPY LIQQGFLQRT PRGRVATLLT YRHFGLSAPA AGSAEGSMWN TPDGA

NT Sequence

ATGATTGAAA CCGACAAACT CGCCGCCGAG CGGATCATCG CCGCCACGCC CGCCTCGTCG CACGAGGAGG TGTTCGAACG CGCGCTGCGG CCGCGCCAGC TCGACGATTA CGTCGGCCAG GAGAAGGTGC GCGGTCAGCT CGAGATCTTC ATCGAGGCCG CGAAACGCCG CTCCGAGCCG CTCGACCACG TGCTGCTGTT CGGCCCGCCG GGCCTCGGCA AGACCACGCT CGCGCACATC ATCGCGCGCG AAATGGGCGT GAATCTGCGC CAGACGTCGG GCCCGGTGCT CGAGCGCGCC GGCGACCTCG CCGCGCTGCT GACCAATCTC GAAGCGAACG ACGTGCTGTT CATCGACGAG ATCCACCGGT TGTCGCCGGT CGTCGAGGAA ATCCTGTATC CGGCGCTCGA GGATTACCAG ATCGACATCA TGATCGGCGA AGGTCCGGCC GCGCGCAGCG TGAAGCTCGA TCTGCAGCCG TTCACGCTGG TGGGCGCAAC GACGCGCGCG GGGATGCTCA CCAATCCGCT GCGCGATCGC TTCGGGATCG TCGCGCGGCT GGAGTTCTAC GACGCCGAGC AGCTGTCGCG CATCGTGCGG CGCTCGGCGG CACTGCTGAA CGCGCAGATC GACCCGAACG GCGCGCTGGA AATCGCGAAG CGCTCGCGCG GCACGCCGCG GATCGCGAAC CGGTTGCTGC GCCGCGTGCG CGACTACGCG GAAGTGAAGG CCGACGGACA GATCACCGCG GCCGTCGCCG ATGCCGCGCT CGCGATGCTC GACGTCGATC CGGTCGGCTT CGACCTGATG GACCGCAAGC TGCTCGAAGC CATCCTGCAC AAGTTCGACG GCGGCCCGGT CGGCATCGAC AACCTCGCGG CCGCGATCGG CGAGGAGCGC GACACGATCG AGGACGTGCT CGAACCGTAC CTGATCCAGC AAGGCTTCCT GCAGCGCACG CCGCGCGGGC GCGTCGCGAC GCTGCTCACC TACCGCCATT TCGGGCTGTC CGCGCCGGCG GCCGGCAGTG CGGAAGGCAG CATGTGGAAC ACGCCCGACG GCGCG

Details for BuviA.00013.a.B1.GE38075

HARVESTED ON:	5/13/2014
SEQUENCED ON:	5/6/2014
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

atcttactca cttacgcgcc gtcgggcgtg ttccacatgc tgccttccgc actgccggcc gccggcgcgg acagcccgaa atggcggtag gtgagcagcg tcgcgacgcg cccgcgcggc gtgcgctgca ggaagccttg ctggatcagg tacggttcga gcacgtcctc gatcgtgtcg cgctcctcgc cgatcgcggc cgcgaggttg tcgatgccga ccgggccgcc gtcgaacttg tgcaggatgg cttcgagcag cttgcggtcc atcaggtcga agccgaccgg atcgacgtcg agcatcgcga gcgcggcatc ggcgacggcc gcggtgatct gtccgtcggc cttcacttcc gcgtagtcgc gcacgcggcg cagcaaccgg ttcgcgatcc gcggcgtgcc gcgcgagcgc ttcgcgattt ccagcgcgcc gttcgggtcg atctgcgcgt tcagcagtgc cgccgagcgc cgcacgatgc gcgacagctg ctcggcgtcg tagaactcca gccgcgcgac gatcccgaag cgatcgcgca gcggattggt gagcatcccc gcgcgcgtcg ttgcgcccac cagcgtgaac ggctgcagat cgagcttcac gctgcgcgcg gccggacctt cgccgatcat gatgtcgatc tggtaatcct cgagcgccgg atacaggatt tcctcgacga ccggcgacaa ccggtggatc tcgtcgatga acagcacgtc gttcgcttcg agattggtca gcagcgcggc gaggtcgccg gcgcgctcga gcaccgggcc cgacgtctgg cgcagattca cgcccatttc gcgcgcgatg atgtgcgcga gcgtggtctt gccgaggccc ggcgggccga acagcagcac gtggtcgagc ggctcggagc ggcgtttcgc ggcctcgatg aagatctcga gctgaccgcg caccttctcc tggccgacgt aatcgtcgag ctggcgcggc cgcagcgcgc gttcgaacac ctcctcgtgc gacgaggcgg gcgtggcggc gatgatccgc tcggcggcga gtttgtcggt ttcaatcata tggtggtg

Expected Protein Sequence

MAHHHHHHMI ETDKLAAERI IAATPASSHE EVFERALRPR QLDDYVGQEK VRGQLEIFIE AAKRRSEPLD HVLLFGPPGL GKTTLAHIIA REMGVNLRQT SGPVLERAGD LAALLTNLEA NDVLFIDEIH RLSPVVEEIL YPALEDYQID IMIGEGPAAR SVKLDLQPFT LVGATTRAGM LTNPLRDRFG IVARLEFYDA EQLSRIVRRS AALLNAQIDP NGALEIAKRS RGTPRIANRL LRRVRDYAEV KADGQITAAV ADAALAMLDV DPVGFDLMDR KLLEAILHKF DGGPVGIDNL AAAIGEERDT IEDVLEPYLI QQGFLQRTPR GRVATLLTYR HFGLSAPAAG SAEGSMWNTP DGA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg aaaccgacaa actcgccgcc gagcggatca tcgccgccac gcccgcctcg tcgcacgagg aggtgttcga acgcgcgctg cggccgcgcc agctcgacga ttacgtcggc caggagaagg tgcgcggtca gctcgagatc ttcatcgagg ccgcgaaacg ccgctccgag ccgctcgacc acgtgctgct gttcggcccg ccgggcctcg gcaagaccac gctcgcgcac atcatcgcgc gcgaaatggg cgtgaatctg cgccagacgt cgggcccggt gctcgagcgc gccggcgacc tcgccgcgct gctgaccaat ctcgaagcga acgacgtgct gttcatcgac gagatccacc ggttgtcgcc ggtcgtcgag gaaatcctgt atccggcgct cgaggattac cagatcgaca tcatgatcgg cgaaggtccg gccgcgcgca gcgtgaagct cgatctgcag ccgttcacgc tggtgggcgc aacgacgcgc gcggggatgc tcaccaatcc gctgcgcgat cgcttcggga tcgtcgcgcg gctggagttc tacgacgccg agcagctgtc gcgcatcgtg cggcgctcgg cggcactgct gaacgcgcag atcgacccga acggcgcgct ggaaatcgcg aagcgctcgc gcggcacgcc gcggatcgcg aaccggttgc tgcgccgcgt gcgcgactac gcggaagtga aggccgacgg acagatcacc gcggccgtcg ccgatgccgc gctcgcgatg ctcgacgtcg atccggtcgg cttcgacctg atggaccgca agctgctcga agccatcctg cacaagttcg acggcggccc ggtcggcatc gacaacctcg cggccgcgat cggcgaggag cgcgacacga tcgaggacgt gctcgaaccg tacctgatcc agcaaggctt cctgcagcgc acgccgcgcg ggcgcgtcgc gacgctgctc acctaccgcc atttcgggct gtccgcgccg gcggccggca gtgcggaagg cagcatgtgg aacacgcccg acggcgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat